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Expression and role of autophagy related protein p62 and LC3 in the retina in a rat model of acute ocular hypertension

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International Journal of Ophthalmology(English edition) 2020年第1期13卷 21-28页

作者： yu-yu Wu Bing-ru zheng Wan-Zhu Chen Mao-Sheng Guo Yi-Hong Huang Yan Zhang Department of Ophthalmology the Second Affiliated Hospital of Fujian Medical University

AIM: To investigate the expression and possible role of the autophagy related protein p62 and LC3 in the retina based on a rat model of acute ocular ***: Fifty rats were randomized into five groups: control group A, B, C, and D. Groups A to D all received normal saline perfusion into the anterior chamber with pressure of 80 mm Hg for one hour, and retina tissue was obtained at 6, 12, 24 and 48 h after perfusion respectively, to investigate the activation of autophagy following ischemiareperfusion. The distribution and semi-quantification of autophagy related protein p62 and LC3 in the retina were detected using immunohistochemistry technique. The expression level of these two proteins was evaluated using Western ***: The number of retinal ganglion cells(RGCs) decreased with increasing reperfusion time, and significant reduction in the retinal thickness was observed 48 h after perfusion. In normal adult rats, LC3 protein was mainly expressed in the ganglion cell layer(GCL), and p62 protein was expressed in the nerve fiber layer(NFL), GCL, inner plexiform layer(IPL), inner nuclear layer(INL) and outer plexiform layer(OPL). In comparison to the control group, the expression level of LC3-II was higher in all the experimental groups(P<0.05), with the peak expression at 12 h after reperfusion. Additionally, the expression level of p62 was higher in all the experimental groups than the control(P<0.05, except for group A), with the peak level occurred 24 h after reperfusion. CONCLUSION: Both p62 and LC3 show low level and uneven expression in the retina of normal adult rats. Acute ocular hypertension can lead to upregulation of LC3-II and p62 expression in the retina. Autophagy flux is damaged 12 h after reperfusion, potentially resulting in further loss of RGCs.

关键词： glaucoma acute ocular hypertension LC3 p62 autophagy

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Computer-assisted retention prediction system in reversed-phase HPLC for O-ethyl O-aryl N-isopropyl phosphoramidothioates

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Chinese Journal of Chemistry 1991年第5期9卷 435-440页

作者： WANG Qin-Sun GAO ru-yu YANG Hua-zheng YAN Bing-Wen National Laboratory of Elemento-Organic Chemistry Nankai UniversityTianjin 300071

A computer-assisted retention prediction system (RPS) of fifteen O-ethyl O-aryl N-iso- propyl phosphoramidothioates (1) in reversed-phase HPLC was investigated. The system is based on the use of four physicochemical parameters (hydrophobicity , electric effect σ, field effect F and steric effect ) which is closely related to the retention mechanism in reversed-phase HPLC. The system was evaluated by comparing the measured retention data with the predicted ones. The predicted values were consistent with the measured values within a relative error of 11.5%.

关键词： Computer-assisted retention prediction system in reversed-phase HPLC for O-ethyl O-aryl N-isopropyl phosphoramidothioates HPLC

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A Cache Replacement Policy Based on Multi-Factors for Named Data Networking

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Computers, Materials & Continua 2020年第10期65卷 321-336页

作者： Meiju yu ru Li yuwen Chen College of Computer Science Inner Mongolia UniversityHohhot020021China Department of Computer Systems Technology New York City College of Technology-The City University of New YorkBrooklynNY 11201USA

Named Data Networking(NDN)is one of the most excellent future Internet architectures and every router in NDN has the capacity of caching contents passing *** greatly reduces network traffic and improves the speed of content distribution and *** order to make full use of the limited caching space in routers,it is an urgent challenge to make an efficient cache replacement ***,the existing cache replacement policies only consider very few factors that affect the cache *** this paper,we present a cache replacement policy based on multi-factors for NDN(CRPM),in which the content with the least cache value is evicted from the caching *** fully analyzes multi-factors that affect the caching performance,puts forward the corresponding calculation methods,and utilize the multi-factors to measure the cache value of ***,a new cache value function is constructed,which makes the content with high value be stored in the router as long as possible,so as to ensure the efficient use of cache *** simulation results show that CPRM can effectively improve cache hit ratio,enhance cache resource utilization,reduce energy consumption and decrease hit distance of content acquisition.

关键词： Cache replacement policy named data networking content popularity freshness energy consumption

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Microbiological indication of municipal solid waste landfill non-stabilization

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Journal of Environmental Sciences 2004年第4期16卷 662-665页

作者： ZHOUQi-xing SYLVESTERrunyuzi yuJi-yu ZHANGQian-ru KeyLaboratoryofTerrestrialEcologicalProcess InstituteofAppliedEcologyChineseAcademyofSciencesShenyang110016China DepartmentofEnvironmentalEngineering ZhejiangUniversityHangzhou310029China TianzilingLandfillCentre Hangzhou310011China

Accidental collapse resulted from unstable factors is an important technological problem to be solved in sanitary landfill. Microbiological degradation of organic matters in landfilled solid waste are an important unstable factor. A landfill reactor was thus manufactured and installed to examine quantitative and population dynamics of microorganisms during degradation of landfilled solid waste. It was showed that unstable landfill can be reflected and indicated by microbiological features such as rapidly decreased growth amount of microorganisms, no detection of fungi and actinomyces, and changing the dominant population into methanogenic bacteria and Acinotobacter.

关键词： municipal solid waste sanitary landfill unstable factor microbiological indication

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Applications of genetic code expansion technology in eukaryotes

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Protein & Cell 2024年第5期15卷 331-363页

作者： Qiao-ru Guo yu J.Cao State Key Laboratory of Chemical Oncogenomic Guangdong Provincial Key Laboratory of Chemical GenomicsPeking University Shenzhen Graduate SchoolShenzhen 518055China Institute of Chemical Biology Shenzhen Bay LaboratoryShenzhen 518132China

Unnatural amino acids(UAAs)have gained significant attention in protein engineering and drug development owing to their ability to introduce new chemical functionalities to *** eukaryotes,genetic code expansion(GCE)enables the incorporation of UAAs and facilitates posttranscriptional modification(PTM),which is not feasible in prokaryotic *** is also a powerful tool for cell or animal imaging,the monitoring of protein interactions in target cells,drug development,and switch ***,there is keen interest in utilizing GCE in eukaryotic *** review provides an overview of the application of GCE in eukaryotic systems and discusses current challenges that need to be addressed.

关键词： genetic code expansion unnatural amino acid eukaryotes basic research therapeutic applications

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Microwave assisted atmospheric acid leaching of nickel from laterite ore

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Rare Metals 2010年第3期29卷 327-332页

作者： CHE Xiaokui SU Xiuzhu CHI ru'an yu Junxia College of Resources and Civil Engineering Northeastern University Shenyang 110004 China Division of Mineral Resources Metallurgy & Materials General Research Institute for Nonferrous Metals Beijing 100088 China Key Laboratory for Green Chemical Process of the Ministry of Education of China & Hubei Key Lab of Novel Reactor & Green Chemical Technology Wuhan Institute of Technology Wuhan 430073 China

The recovery of nickel from laterite ore with sulphuric acid under the effect of microwave irradiation was *** experimental results indicated that the extraction rate of nickel was influenced by reaction time,sulphuric acid concentration,and temperature,especially by microwave *** results obtained from the experiments of orthogonal arrays showed that the optimum conditions of sulphuric acid concentration,reaction time,microwave power,and temperature were 25 vol.%,1.5 h,600 W,and 90°C,*** the optimal conditions,the nickel recovery could reach approximately 90.8%,which was higher than that obtained by conventional water bath *** experiments showed that the leaching of nickel in a sulphuric acid medium was controlled by chemical reaction occurring on the surface of laterite *** apparent activation energy was 38.9 kJ/*** heating technology is efficient,clean,and easy to control and facilitate continuous processing of materials.

关键词： microwave sulphuric acid-leaching laterite ore nickel

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Complete genome sequence of Bacillus amyloliquefaciens YP6, a plant growth rhizobacterium efficiently degrading a wide range of organophosphorus pesticides

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Journal of Integrative Agriculture 2019年第11期18卷 2668-2672页

作者： MENG Di ZHAI Li-xin TIAN Qiao-peng GUAN zheng-bing CAI yu-jie LIAO Xiang-ru Key Laboratory of Industrial Biotechnology Ministry of Education School of Biotechnology Jiangnan University

Bacillus amyloliquefaciens YP6,a plant growth promoting rhizobacteria,is capable of efficiently degrading a wide range of organophosphorus pesticides(OPs).Here,we report the complete genome sequence of this bacterium with a genome size of 4009619 bp,4210 protein-coding genes and an average GC content of 45.9%.Based on the genome sequence,several genes previously described as being involved in solubilizing-phosphorus,OPs-degradation,indole-3-acetic acid(IAA)and siderophores ***,compared with the genomes of *** species,strain YP6 had larger genome size and the most protein-coding ***,the four categories of“cell envelope biogenesis,outer membrane(M),”“translation,ribosomal structure and biogenesis(J),”“transcription(K),”and“signal transduction mechanisms(T)”were *** differences may be related to extensive environmental adaptability of the genus *** results expand the application potential of strain YP6 for environmental bioremediation,provide gene resources involved in OPs degradation for biotechnology and gene engineering,and contribute to provide insights into the relationship between microorganism and living environment.

关键词： organophosphorus pesticides genome sequence plant growth promoting rhizobacteria Bacillus amyloliquefaciens

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Disulfide-stabilized single-chain antibody-targeted superantigen: Construction of a prokaryotic expression system and its functional analysis

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World Journal of Gastroenterology 2005年第31期11卷 4899-4903页

作者： Jian-Li Wang yu-Ling zheng ru Ma Bao-Li Wang Ai-Guang Guo Yong-Qiang Jiang Department of Life Science Northwest Sci-Tech University of Agriculture and ForestryNo. 22 Xi nong Road Yangling 712100 Shaanxi Province China Institute of Microbiology and Epidemiology Academy of Military Medical Sciences No. 20 Dongda Street Fengtai District Beijing 100071China

AIM: To construct the expression vector of B3 (scdsFv)-SEA (D227A) and to identify its binding and cytotoxic ability to B3 antigen positive carcinoma cell ***: This fusion protein was produced by a bacterial expression system in this study. It was expressed mainly in the inclusion body. The gene product was solubilized by guanidine hydrochloride, refolded by conventional dilution method, and purified using SP-sepharose cation ***: The expression vector B3 (scdsFv)-SEA-PETwas constructed, the expression product existed mainly in the inclusion body, the refolding product retained the binding ability of the single-chain antibody and had cytotoxic effect on HT-29 colon carcinoma cells. The stability assay showed that the resulting protein was stable at 37 ℃.CONCLUSION: This genetically engineered B3 (scdsFv)-SEA fusion protein has bifunction of tumor targeting and tumor cell killing and shows its promises as an effective reagent for tumor-targeted immunotherapy.

关键词： B3 monoclonal antibody Single-chain disulfide-stabilized Fv Superantigen

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Enhancing coercivity and thermal stability of(Nd,Y)-Fe-B sintered magnets through lamellar structure design

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Rare Metals 2024年第12期43卷 6571-6582页

作者： Xiao-Dong Fan yu-Hao Li Shuai Cao Guang-Fei Ding Shuai Guo Bo zheng Ren-Jie Chen A.ru Yan CISRI & NIMTE Joint Innovation Center for Rare Earth Permanent Magnets Ningbo Institute of Materials Technology and EngineeringChinese Academy of Sciences School of Materials Science and Chemical Engineering Ningbo University University of Chinese Academy of Sciences

The incorporation of the high-abundance rareearth element Y in(Nd,Y)-Fe-B sintered magnets offers an opportunity to reduce the cost of permanent magnetic materials,while promoting the balanced usage of rare-earth ***,the performance of(Nd,Y)-Fe-B magnets prepared using the conventional dual-main-phase(DMP) method undergoes significant degradation due to the strong diffusion ability of *** suppress the excessive diffusion of Y,this study presents a macroscopic lamellar magnet preparation ***,the micromagnetic simulations revealed that the multilayer magnets exhibited superior intrinsic performance compared to DMP ***,the multilayer magnets were prepared by alternately stacking the 0% Y(0Y) and 30% Y(30Y) magnetic *** observed magnetic properties demonstrated that the coerc)vity of the three-layer magnet was～0.23 T higher than that of the DMP magnet,leading to improved coercivity stability at high ***,the microstructural observations and elemental analyses indicated the presence of a～200-μm-thick interface layer at the contact site between the 0Y and 30Y magnetic ***,the proposed approach effectively suppressed the excessive diffusion of Y in(Nd,Y)-Fe-B magnets,thereby enhancing the magnetic performance of the sintered magnets.

关键词： Coercivity Lamellar structure design Nd–Y–Fe–B magnets Thermal stability

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Severe fundus lesions induced by ocular jellyfish stings: A case report

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World Journal of Clinical Cases 2020年第19期8卷 4544-4549页

作者： Xiang-yue zheng De-Jin Cheng Li-Hua Lian ru-Ting Zha ng Xiao-Yi yu Department of Ophthalmology First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510405Guangdong ProvinceChina Department of Nephrology First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510405Guangdong ProvinceChina

BACKGROUND Globally,although the jellyfish population has increased in recent years,ocular jellyfish stings remain an uncommon ophthalmic emergency,and have been rarely *** to a few previous reports,ocular jellyfish stings may cause anterior segment disorders,and most of these injuries were self-limited and spontaneously resolved within 24 to 48 *** SUMMARY A brother and sister both presented with severe fundus complications several years after ocular jellyfish stings and both had prolonged blurred *** our knowledge,such fundus lesions induced by jellyfish stings have not been reported *** The fundus status of patients following ocular jellyfish stings should be carefully monitored in cases of irreversible ocular damage.

关键词： Ocular jellyfish stings Fundus lesions Complications Prolonged blurred vision Case report

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