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Journal of environmental sciences 2009年第12期21卷 1667-1672页

作者： zheng weishuang, fei yingheng,{2., peking university, beijing 100871, china. [a] college of environmental {2. engineering Peking University Beijing 100871 China

Fungi and their symbionts can alleviate heavy metal stress by exuding soluble proteins and enzymes. This study examined the role of soluble protein and acid phosphatase （APase） exuded by Xerocomus chrysenteron, an ectomycorrhizal fungus, and the seedlings of its symbiont, Chinese pine （Pinus tabulaeformis）, under conditions of excessive Cu and Cd. The growth type showed that this poorly studied ectomycorrhizal fungus was capable of tolerating high concentrations of Cu, and may be useful in phytoremediation. X. chrysenteron grew well at 80 mg/L Cu, and the EC50 for Cd was 17.82.mg/L. X. chrysenteron also showed enhanced exudation of soluble protein in both isolated and inoculated cultivations under the influence of Cu and Cd. Soluble protein exudation, however, differed under Cu and Cd stress in isolates. In mediums containing Cu, soluble protein exudation increased with concentration, but in mediums containing Cd the content of soluble protein increased to a comparable level at all concentrations. This study demonstrated that soluble protein was related to heavy metal tolerance, although the different ions played different roles. While APase activity in exudates of fungi and seedlings decreased under Cu and Cd stress in comparison to the control, the APase activity in seedlings was maintained by inoculation. Thus, X. chrysenteron facilitated the ability of plant to maintain a normal nutrient uptake, and therefore to protect it from heavy metal toxicity.

关键词： soluble protein acid phosphatase exudate Cu, Cd Xerocomus chrysenteron

Enhanced organic degradation and microbial community cooperation by inoculating Bacillus licheniformis in low temperature composting

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Journal of environmental sciences 2024年第9期143卷 189-200页

作者： Jing Su Kaiyun Zhou Wenjie Chen Shaoqi Xu Ziwei Feng Yuan Chang Xiaoyan Ding yi zheng Xingling Tao Ake Zhang Yue Wang Ji Li Guochun Ding Yuquan Wei Nanjing Institute of environmental sciences Ministry of Ecology and EnvironmentNanjing 210042China college of Resources and environmental Science Beijing Key Laboratory of Biodiversity and Organic FarmingChina Agricultural UniversityBeijing 100193China Organic Recycling Institute(Suzhou)of china.Agricultural university Wuzhong DistrictSuzhou 215128China Fuyang Academy of Agricultural sciences Fuyang 236065China

Microbial activity and interaction are the important driving factors in the start-up phase of food waste composting at low *** aim of this study was to explore the effect of inoculating Bacillus licheniformis on the degradation of organic components and the potential microbe-driven mechanism from the aspects of organic matter degradation,enzyme activity,microbial community interaction,and microbial metabolic *** results showed that after inoculating ***,temperature increased to 47.8℃ on day2.and the degradation of readily degraded carbohydrates(RDC)increased by 31.2.,and the bioheat production increased by 16.5%.There was an obvious enhancement of extracellular enzymes activities after inoculation,especially amylase activity,which increased by 7.68 times on day *** inoculated *** colonized in composting as key genus in the start-up *** network analysis and Mantel test indicated that inoculation drove the cooperation between microbial network modules who were responsible for various organic components(RDC,lipid,protein,and lignocellulose)degradation in the start-up *** function prediction suggested that carbohydrate metabolisms including starch and sucrose metabolism,glycolysis/gluconeogenesis,pyruvate metabolism,etc.,were improved by increasing the abundance of related functional genes after *** conclusion,inoculating *** accelerated organic degradation by driving the cooperation between microbial network modules and enhancing microbial metabolism in the start-up phase of composting.

关键词： Microbial agent Organic matter degradation Microbial interaction Food waste Low temperature composting

西蒙得木HSP2.基因家族的进化、表达和功能分析

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生物技术通报 2024年第6期40卷 190-202页

作者：李博静郑腊梅吴乌云高飞周宜君中央民族大学生命与环境科学学院北京100081

【目的】研究西蒙得木HSP2.家族成员的结构和功能,为后续研究提供支撑。【方法】从结构分析、系统发育、表达模式等方面对西蒙得木HSP2.家族成员(ScHSP2.s)进行系统分析,并对ScHSP2.-17进行功能分析。【结果】西蒙得木基因组中包含35个H... 详细信息

【目的】研究西蒙得木HSP2.家族成员的结构和功能,为后续研究提供支撑。【方法】从结构分析、系统发育、表达模式等方面对西蒙得木HSP2.家族成员(ScHSP2.s)进行系统分析,并对ScHSP2.-17进行功能分析。【结果】西蒙得木基因组中包含35个HSP2.基因座位,其中8个基因涉及串联重复,5个涉及片段重复。系统发育分析表明,35个ScHSP2.聚为12.亚家族,其中核质亚家族数量最多。启动子分析表明,多数ScHSP2.家族成员启动子区域含有激素响应和非生物胁迫等相关顺式作用元件。转录组分析表明,ScHSP2.-35和ScHSP2.-17等部分热激蛋白基因在花和种子发育过程中表达量发生显著性改变,而ScHSP2.-17和ScHSP2.-2.等基因受高温诱导。亚细胞定位分析表明,ScHSP2.-17编码的蛋白定位于细胞核中,并且在大肠杆菌、酵母和烟草中过表达ScHSP2.-17能显著提高它们对高温胁迫的耐受性。【结论】部分ScHSP2.家族成员参与了西蒙得木生殖发育和非生物胁迫应答。

关键词：西蒙得木 HSP2. 亚细胞定位表达分析高温胁迫

Genome assembly of autotetraploid Actinidia arguta highlights adaptive evolution and enables dissection of important economic traits

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Plant Communications 2024年第6期5卷 173-192页

作者： Xue-Mei Lu Xiao-Fen Yu Guo-Qiang Li Ming-Hao Qu Huan Wang Chuang Liu Yu-Ping Man Xiao-Han Jiang Mu-Zi Li Jian Wang Qi-Qi Chen Rui Lei Cheng-Cheng Zhao Yun-Qiu Zhou zheng-Wang Jiang Zuo-Zhou Li Shang zheng Chang Dong Bai-Lin Wang Yan-Xiang Sun Hui-Qin Zhang Jie-Wei Li Quan-Hui Mo ying Zhang Xin Lou Hai-Xu Peng Ya-Ting yi He-Xin Wang Xiu-Jun Zhang yi-Bo Wang Dan Wang Li Li Qiong Zhang Wen-Xia Wang Yongbo Liu Lei Gao Jin-Hu Wu Yan-Chang Wang CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of SciencesWuhanHubeiChina Key Laboratory of Western china.s environmental Systems(Ministry of Education) College of Earth and Environmental SciencesLanzhou UniversityLanzhouGansuChina college of Life sciences University of Chinese Academy of SciencesBeijingChina Wuhan Frasergen Bioinformatics Co. LtdWuhanHubeiChina Institute of Soil and Fertilizer Anhui Academy of Agricultural SciencesHefeiChina college of Agricultural sciences Xichang UniversityXichangSichuanChina Department of Horticulture Heilongjiang Academy of Agricultural SciencesHarbinHeilongjiangChina college of Life sciences Langfang Normal UniversityLangfangHebeiChina Institute of Horticulture Zhejiang Academy of Agricultural SciencesHangzhouZhejiangChina Guangxi Institute of Botany Chinese Academy of SciencesGuilinGuangxiChina Xi’an Botanical Garden of Shaanxi Province Institute of Botany of Shaanxi ProvinceXi’anShaanxiChina Institute of Modern Agricultural Research Dalian UniversityDalianLiaoningChina Bioinformatics Center College of Plant Science and TechnologyBeijing University of AgricultureBeijingChina college of Agriculture Eastern Liaoning UniversityDandongLiaoningChina Dalian Institute of Chemical Physics Chinese Academy of SciencesDalianLiaoningChina State environmental Protection Key Laboratory of Regional Eco-process and Function Assessment Chinese Research Academy of Environmental SciencesBeijingChina Hubei Hongshan Laboratory WuhanHubeiChina The New Zealand Institute for Plant and Food Research Limited AucklandNew Zealand

Actinidia arguta,the most widely distributed Actinidia species and the second cultivated species in the genus,can be distinguished from the currently cultivated Actinidia chinensis on the basis of its small and smooth fruit,rapid softening,and excellent cold *** evolution of tetraploid Actinidia species and the genetic basis of their important agronomic traits are still ***,we generated a chromosome-scale genome assembly of an autotetraploid male *** *** genome assembly was 2.77 Gb in length with a contig N50 of 9.97 Mb and was anchored onto 116 *** and clustering of 101 geographically representative accessions showed that they could be divided into two geographic groups,Southern and Northern,which first diverged 12.9 million years *** underwent two prominent expansions and one demographic bottleneck from the midPleistocene climate transition to the late *** genomics studies using paleoclimate data enabled us to discern the evolution of the species’adaptation to different historical *** genes(AaCEL1,AaPME1,and AaDOF1)related to flesh softening were identified by multi-omics analysis,and their ability to accelerate flesh softening was verified through transient expression assays.A set of genes that characteristically regulate sexual dimorphism located on the sex chromosome(Chr3)or autosomal chromosomes showed biased expression during stamen or carpel *** chromosome-level assembly of the autotetraploid *** genome and the genes related to important agronomic traits will facilitate future functional genomics research and improvement of ***.

关键词： kiwiberry dioecism adaptive evolution paleoclimate GEA rapid softening

灌丛斑块和草本斑块碳交换对季节性降水增加的响应——基于地上净初级生产力和叶面积指数标准化的比较分析

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植物生态学报 2024年第8期48卷 1035-1049页

作者：张梦迪向官海文艺瑶王欢呼格吉勒白永飞王忠武郑淑霞内蒙古农业大学草原与资源环境学院呼和浩特010018 中国科学院植物研究所植被与环境变化国家重点实验室北京100093 赤峰学院农学院内蒙古赤峰024000 国家植物园北京100093 江西师范大学生命科学学院南昌330022 山东农业大学生命科学学院山东泰安271001 正蓝旗草原工作站锡林浩特027200

随着气候变化和人类活动干扰的加剧,干旱半干旱地区草地灌丛化现象普遍发生,严重影响了草地生态系统的碳汇功能。水分是内蒙古半干旱草原的主要限制因子,未来降水格局的变化对草原生态系统的碳交换具有重要影响。然而,目前关于降水变化... 详细信息

随着气候变化和人类活动干扰的加剧,干旱半干旱地区草地灌丛化现象普遍发生,严重影响了草地生态系统的碳汇功能。水分是内蒙古半干旱草原的主要限制因子,未来降水格局的变化对草原生态系统的碳交换具有重要影响。然而,目前关于降水变化对灌丛化草地生态系统,特别是异质斑块碳交换过程的影响研究较少,相关机制尚不清楚。为此,该研究利用内蒙古小叶锦鸡儿(Caragana microphylla)灌丛化草地季节性降水增加(冬季增雪、夏季增雨)实验平台,系统观测了灌丛斑块和草本斑块的碳交换参数,即净生态系统碳交换(NEE)、总生态系统生产力(GEP)、生态系统呼吸(ER),并结合基于地上净初级生产力(ANPP)和叶面积指数(LAI)标准化的参数比较分析,研究了季节性降水增加对灌丛化草地碳交换的影响以及异质斑块的响应差异。结果表明:1)夏季增雨显著提高了草本斑块|NEE|、GEP和ER值,而冬季增雪显著降低了草本斑块|NEE|ANPP、GEPANPP和ERANPP。夏季增雨显著增加了灌丛斑块GEP和ER值,但对NEE影响不明显,冬季增雪对灌丛斑块的碳交换过程有促进作用。总体而言,灌丛斑块的|NEE|、GEP、ER显著高于草本斑块。相较于湿润年份(2.2.年),干旱年份(2.2.年)的碳交换对降水增加的响应更为敏感。2.灌丛斑块碳交换(|NEE|、GEP和ER)与土壤水分含量、叶片生物量呈正相关关系,夏季增雨主要通过增加深层土壤(40-80 cm)水分含量、降低土壤温度来促进碳交换。草本斑块碳交换与浅层土壤(0-2. cm)水分含量、ANPP呈正相关关系,与土壤温度、根冠比呈负相关关系;夏季增雨主要通过增加浅层土壤水分含量、降低土壤温度促进碳交换,而冬季增雪则通过增加深层土壤水分含量和减少地下生物量来抑制草本斑块碳交换。3)基于ANPP标准化后的碳交换参数能更好地揭示灌丛斑块和草本斑块对降水变化的响应差异。该研究结果为准确评估气候变化下干旱半干旱草地生态系统的碳汇功能和固碳潜力提供了重要科学依据。

关键词：净生态系统碳交换(NEE) 总生态系统生产力(GEP) 生态系统呼吸(ER) 灌丛斑块草本斑块冬季增雪夏季增雨灌丛化

Assessing Landsat-8 and Sentinel-2.spectral-temporal features for mapping tree species of northern plantation forests in Heilongjiang Province,china

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Forest Ecosystems 2022年第3期9卷 344-356页

作者： Mengyu Wang yi zheng Chengquan {2. Ran Meng Yong Pang Wen Jia Jie Zhou Zehua {2. Linchuan Fang Feng Zhao Key Laboratory of Geographical Process Analysis&Simulation of Hubei Province/college of Urban and environmental {2. Central China Normal UniversityWuhan430079China Department of Geographical sciences University of MarylandCollege ParkMD20742USA Macro Agriculture Research Institute Interdisciplinary Sciences Research InstituteCollege of Resources and EnvironmentHuazhong Agricultural UniversityWuhan430070China Institute of Forest Resource Information Techniques Chinese Academy of ForestryBeijing100091China Key Laboratory of Forestry Remote Sensing and Information System National Forestry and Grassland AdministrationBeijing100091China

Background:Accurate mapping of tree species is highly desired in the management and research of plantation forests,whose ecosystem services are currently under ***-series multispectral satellite images,e.g.,from Landsat-8(L8)and Sentinel-2.S2.,have been proven useful in mapping general forest types,yet we do not know quantitatively how their spectral features(e.g.,red-edge)and temporal frequency of data acquisitions(e.g.,16-day vs.5-day)contribute to plantation forest mapping to the species ***,it is unclear to what extent the fusion of L8 and S2.will result in improvements in tree species mapping of northern plantation forests in ***:We designed three sets of classification experiments(i.e.,single-date,multi-date,and spectral-temporal)to evaluate the performances of L8 and S2.data for mapping keystone timber tree species in northern *** first used seven pairs of L8 and S2.images to evaluate the performances of L8 and S2.key spectral features for separating these tree species across key growing *** we extracted the spectral-temporal features from all available images of different temporal frequency of data acquisition(i.e.,L8 time series,S2.time series,and fusion of L8 and S2.to assess the contribution of image temporal frequency on the accuracy of tree species mapping in the study ***:1)S2.outperformed L8 images in all classification experiments,with or without the red edge bands(0.4%–3.4%and 0.2.–4.4%higher for overall accuracy and macro-F1,respectively);2.NDTI(the ratio of SWIR1 minus SWIR2.to SWIR1 plus SWIR2.and Tasseled Cap coefficients were most important features in all the classifications,and for time-series experiments,the spectral-temporal features of red band-related vegetation indices were most useful;3)increasing the temporal frequency of data acquisition can improve overall accuracy of tree species mapping for up to 3.2.(from 90.1%using single-date imagery to 93.3%using S2.time-series),yet similar ov

关键词： Tree species mapping Plantation forests Red-edge features Temporal frequency of data acquisition Fusion of Landsat-8 and Sentinel-2

Advances in interscale and interdisciplinary approaches to the South china.Sea

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Acta Oceanologica Sinica 2021年第11期40卷 196-199页

作者： Lingling Xie yi Guan Jianyu Hu Quanan zheng Laboratory of Coastal Ocean Variation and Disaster Prediction College of Ocean and MeteorologyGuangdong Ocean UniversityZhanjiang 524088China Key Laboratory of Climate Resources and Environments in Continent Shelf Sea and Deep OceanZhanjiang 524088China Key Laboratory of Space Ocean Remote Sensing and Applications Ministry of Natural ResourcesBeijing 100081China State Key Laboratory of Marine environmental Science College of Ocean and Earth SciencesXiamen UniversityXiamen 361005China Department of Atmospheric and Oceanic Science University of MarylandCollege Park 20742USA

The South china.Sea(SCS)connects the Pacific Ocean and the Indian Ocean,and acts as an important part in regional and global climate systems(e.g.,Qu et al.,2.09;Wang et al.,2.09).Multi-scale dynamic and biogeochemical processes in the SCS,comprising a hot spot in marginal sea studies,have attracted great attentions from researchers(e.g.,Chen et al.,2.2.;Hu et al.,2.2.).The South china.Sea Annual Meeting(SCSAM)2.2.,recently held on October 2.–2. in Zhanjiang,{2.focused on academic exchanges of the newly research results and progresses in the interdisciplinary multi-scale processes in the *** SCSAM 2.2. is the eighth international workshop of the series,which started in April 2.13(Zhu,2.13)and renamed as SCSAM in *** were 90 oral presentations and 57 posters in the meeting this year,which attracted attentions of more than 2.00 audiences both on line and on *** short article summaries the cutting-edge advances in interscale and interdisciplinary approaches to the SCS from the meeting presentations and the associated research.

关键词： South Indian marginal

Vegetable and Fruit Consumption among Chinese Adults and Associated Factors： A Nationally Representative Study of 170,847 Adults

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Biomedical and environmental sciences 2017年第12期30卷 863-874页

作者： LI yi Chong JIANG Bo ZHANG Mei huang {2. Jing DENG Qian ZHOU Mai Geng ZHAO Zhen Ping WANG You Fa WANG Li Min National Center for Chronic Non-communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100050 China peking university Clinical Research Institute Beijing 100191 China beijing Center for Disease Prevention and Control Beijing 100191 China Global Health Institute Health Science Center Xi 'an Jiaotong University Xi'an 710049 Shanxi China Fisher Institute of Health and Well-being Systems-Oriented Global Childhood Obesity Intervention Program Department of Nutrition and Health Sciences College of Health Ball State University Muncie IN 47306 USA

Objective This study examined vegetable and fruit （VF） consumption rate and its associated factors among Chinese adults. Methods Nationally representative data from the 2.13 china.Chronic Disease Surveillance survey were used. Dietary intake data, including VF consumption during the last 12.months, were collected. All analyses were weighted to obtain nationally representative estimates. Associations between VF consumption and other factors （e.g., meal frequency and physical activity） were examined through logistic regression analysis. Results The average fruit consumption was 102.3 g/day （95% CI： 97.0-107.6） and the average vegetable consumption was 350.6 g/day （95% CI： 339.3-361.8）. Over half （53.2., 95% CI： 50.9-55.4） of Chinese adults met the VF consumption of 400 g/day recommended by the World Health Organization （WHO）. Rural residents had a higher prevalence of low VF consumption rate than urban residents [49.2.% （95% CI： 46.2.-52.2.） vs. 44.0% （95% CI： 41.7%-46.3%） P 〈 0.01]. Old age （OR = 1.01, 95% CI： 1.00-1.01）, low educational level, low income, minority ethnicity （OR = 1.41, 95% CI： 1.15-1.74）, underweight （OR = 1.17, 95% CI： 1.03-1.33）, single marital status （OR = 1.2., 95% CI： 1.08-1.33）, low health literacy, irregular breakfast （OR = 1.2., 95% CI： 1.04-1.38） or lunch （OR = 1.58, 95% CI： 1.2.-1.99） habits, and no leisure-time physical activity were associated with low VF consumption. Conclusion Only half of Chinese adults met the VF consumption recommended by the WHO. Low socio-economic status, irregular diet, and poor health literacy were likely associated with low VF consumption. National efforts and programs are needed to promote VF consumption.

关键词： Fruit Vegetables china.Noncommunicable and Chronic Disease Surveillance survey china

利用含油培养基富集、分离和评估海洋产聚羟基脂肪酸酯细菌

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微生物学通报 2021年第12期48卷 4507-4520页

作者：郑维爽于盛洋翟生强黄艺北京大学深圳研究院海洋生物资源与环境研究所广东深圳518057 深港产学研基地(北京大学香港科技大学深圳研修院) 广东深圳518057 北京大学环境科学与工程学院北京100871

【背景】细菌可通过脂肪酸代谢途径耦合聚羟基脂肪酸酯(Polyhydroxyalkanoate,PHA)合成途径实现合成中长链PHA;其拉伸强度、玻璃化温度等加工特性均优于短链PHA,是未来工业化PHA材料的发展方向。【目标】为了获得新的可代谢油类的产PHA... 详细信息

【背景】细菌可通过脂肪酸代谢途径耦合聚羟基脂肪酸酯(Polyhydroxyalkanoate,PHA)合成途径实现合成中长链PHA;其拉伸强度、玻璃化温度等加工特性均优于短链PHA,是未来工业化PHA材料的发展方向。【目标】为了获得新的可代谢油类的产PHA细菌,使用3种分离策略从海洋沉积物中分离和鉴定细菌,并评估菌株产PHA的能力。【方法】采集深圳大鹏湾近海沉积物样品;样品通过直接分离,5周连续培养分离,或10周连续提高盐度(3.5%-12.5%)和含油量(1%-10%)富集培养分离,纯化得到菌株;通过16S rRNA基因相似性及系统进化分析鉴定细菌分类地位,利用PHA聚合酶(PhaC)基因鉴定细菌合成PHA的能力;通过测定基因组框架图,分析细菌的PhaC类型、代谢通路及系统进化关系;通过气相色谱分析细菌产PHA的含量及组成。【结果】从深圳大鹏湾近海底泥样品中分离得到96株细菌,phaC基因阳性率达38%,其中包含9个此前未通过产物证实可产PHA的属,包括尖球菌属(Acuticoccus)、海洋源菌属(Idiomarina)、盐芽孢杆菌属(Halobacillus)、微泡菌属(Microbulbifer)、海棍状菌属(Maritimibacter)、硝酸盐还原菌属(Nitratireductor)、公海橄榄菌属(Pelagibaca)、假海栖菌属(Pseudooceanicola)和海旋菌属(Thalassospira)。除了微泡菌属,其他8个属的细菌通过含油富集法分离获得,说明含油富集方法有利于发现新的产PHA细菌资源。此外,获得2.胞内PHA含量高的菌株。Nitratireductor aquimarinus SY-2.4在营养肉汤中培养可累积细胞干重35.0%的PHA;Roseibium aggregate SN13-2.以丙酮酸盐为碳源,可累积细胞干重19.9%的PHA。【结论】大鹏海域蕴藏着丰富的产PHA细菌资源,值得进一步挖掘研究。梯度增加含油量和盐度的富集方法有利于分离区系不同的产PHA细菌,适用于分离PHA资源。获得2.潜在PHA高产菌,未来将对菌株进行发酵优化研究。

关键词：近海沉积物聚羟基脂肪酸酯中度嗜盐菌含油富集 phaC基因

Biodegradation Mechanism and Technology of Polycyclic Aromatic Hydrocarbons

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科技视界 2016年第25期 99-100页

作者：刁硕 WANG Hong-qi zheng yi-nan HUA {2. 北京师范大学水科学研究院 college of Water sciences Beijing Normal University

Polycyclic aromatic hydrocarbons are a class of potentially hazardous chemicals of environmental and health *** are one of the most prevalent groups of contaminants found in *** of complex hydrocarbon usually requires the cooperation of more than single *** paper reviews the existing screening methods of PAH-degrading *** studied the mechanism and technical applications of the cometabolism in *** gives the suggestions and prospects in Biodegradable trend of PHAs.

关键词： PHAs 生物可分解性共同新陈代谢污染物