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Influence of efflux pump inhibitors on the multidrug resistance of Helicobacter pylori

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World Journal of Gastroenterology 2010年第10期16卷 1279-1284页

作者： Zhang, Zhan Liu, Zhi-Qiang zheng, peng-yuan Tang, Fu-Ai Yang, Ping-Chang zhengzhou Univ Affiliated Hosp 2 Dept Gastroenterol Zhengzhou 450014 Henan Province Peoples R China McMaster Univ Dept Pathol & Mol Med Hamilton ON L8N 4A6 Canada

AIM:To evaluate the effect of efflux pump inhibitors (EPIs) on multidrug resistance of Helicobacter pylori (H. pylori).METHODS: H. pylori strains were isolated and cultured on Brucella agar plates with 10% sheep's blood. The multidrug resistant (MDR) H. pylori were obtained with the inducer chloramphenicol by repeated doubling of the concentration until no colony was seen, then the susceptibilities of the MDR strains and their parents to 9 antibiotics were assessed with agar dilution tests. The present study included periods before and after the advent of the EPIs, carbonyl cyanide m-chlorophenyl hydrazone (CCCP), reserpine and pantoprazole), and the minimum inhibitory concentrations (MICs) were determined accordingly. In the same way, the effects of 5 proton pump inhibitors (PPIs), used in treatment of H. pylori infection, on MICs of antibiotics were ***: Four strains of MDR H. pylori were induced successfully, and the antibiotic susceptibilities of MDR strains were partly restored by CCCP and pantoprazole, but there was little effect of reserpine. Rabeprazole was the most effective of the 5 PPIs which could decrease the MICs of antibiotics for MDR H. pylori ***: In vitro, some EPIs can strengthen the activities of different antibiotics which are the putative substrates of the efflux pump system in H. pylori.

关键词： Multidrug efflux pump Helicobacter pylori Multidrug resistance Proton pump inhibitor Real-time polymerase chain reaction

Species differentiation and identification in the genus of Helicobacter

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World Journal of Gastroenterology 1999年第1期5卷 12-14页

作者： HUA Jie Song, zheng peng yuan and HO Bow

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关键词： Helicobacter genus species biological features biochemical tests identification differentiation

Baicalin Prevents Colon Cancer by Suppressing CDKN2A Protein Expression

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Chinese Journal of Integrative Medicine 2024年第11期30卷 1007-1017页

作者： LI Gang-gang CHU Xiu-feng XING Ya-min XUE Xia Ihtisham Bukhari LIANG Xin-feng XU Ji-xuan MI Yang zheng peng-yuan Henan Key Laboratory of Helicobacter pylori Microbiota and Gastrointestinal CancersMarshall Medical Research CenterFifth Affiliated Hospital of Zhengzhou UniversityZhengzhou 400015China

Objective:To observe the therapeutic effects and underlying mechanism of baicalin against colon ***:The effects of baicalin on the proliferation and growth of colon cancer cells MC38 and *** were observed and predicted potential molecular targets of baicalin for colon cancer therapy were studied by network ***,molecular docking and drug affinity responsive target stability(DARTS)analysis were performed to confirm the interaction between potential targets and ***,the mechanisms predicted by in silico analyses were experimentally verified in-vitro and ***:Baicalin significantly inhibited proliferation,invasion,migration,and induced apoptosis in MC38 and CT26 cells(all P<0.01).Additionally,baicalin caused cell cycle arrest at the S phase,while the G/G,phase was detected in the tiny portion of the *** network pharmacology analysis identified 6 therapeutic targets associated with baicalin,which potentially affect various pathways including 39 biological processes and 99 signaling *** addition,molecular docking and DARTS predicted the potential binding of baicalin with cyclin dependent kinase inhibitor 2A(CDKN2A),protein kinase B(AKT),caspase 3,and mitogen-activated protein kinase(MAPK).In vitro,the expressions of CDKN2A,MAPK,and p-AKT were suppressed by baicalin in MC38 and CT26 *** vivo,baicalin significantly reduced the tumor size and weight(all P<0.01)in the colon cancer mouse model via inactivating p-AKT,CDKN2A,cyclin dependent kinase 4,cyclin dependent kinase 2,interleukin-1,tumor necrosis factorα,and activating caspase 3 and mouse double minute 2 homolog signaling(all P<0.05).Conclusion:Baicalin suppressed the CDKN2A protein level to prevent colon cancer and could be used as a therapeutic target for colon cancer.

关键词： colon cancer baicalin CDKN2A network pharmacology cell cycle apoptosis

Helicobacter pylori acquisition of metronidazole resistance by natural transformation in vitro

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World Journal of Gastroenterology 1998年第5期4卷 22-24页

作者： HUA Jiesong 1, zheng peng yuan 1, Teo Keng Fong 1, Khin Mar Mar 2 and HO Bow 1

AIM To study whether Helicobacter pylori is naturally transformable. METHODS Transformation was performed in BHI broth supplemented with horse serum and yeast extract. Genomic DNA extracted from a metronidazole resistant H. pylori strain was added to H. pylori broth culture. The mixture was incubated at microaerophilic atmosphere. The DNA treated cells were plated on blood agar containing 8mg/L metronidazole to select for transformants. Sterile distilled water was used as a negative DNA control. The DNA profiles of transformants were compared with that of their parent strains by randomly amplified polymorphic DNA (RAPD) fingerprinting. RESULTS Transformation of H. pylori with DNA from a metronidazole resistant strain as a marker was demonstrated. Out of the 12 strains of H. pylori tested, 9 (75%) strains were found to be transformable. The transformation frequencies ranged from 3 4×10 -6 to 2 4×10 -4 . By RAPD, DNA fingerprints of the transformants and their parent strains showed no change in DNA profiles though transformants were all resistant to metronidazole as compared with their metronidazole sensitive parent strains. CONCLUSION Helicobacter pylori is naturally transformable which might be one of the ways that H. pylori develops resistance to metronidazole.

关键词： Helicobacter pylori metronidazole drug resistance, microbial transformation, bacterial

Correlation between the rock mass properties and maximum horizontal stress:A case study of overcoring stress measurements

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 39-48页

作者： peng Li Meifeng Cai Shengjun Miao yuan Li Yu Wang Key Laboratory of Ministry of Education for Efficient Mining and Safety of Metal Mines University of Science and Technology BeijingBeijing 100083China State Key Laboratory of Coal Resources and Safe Mining China University of Mining and TechnologyXuzhou 221116China

Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress *** investigate the correlation between the rock mass properties and maximum horizontal stress,the three-dimensional(3D)stress tensors at 89 measuring points determined using an improved overcoring technique in nine mines in China were adopted,a newly defined characteristic parameter C_(ERP)was proposed as an indicator for evaluating the structural properties of rock masses,and a fuzzy relation matrix was established using the information distribution *** results indicate that both the vertical stress and horizontal stress exhibit a good linear growth relationship with *** is no remarkable correlation between the elastic modulus,Poisson's ratio and depth,and the distribution of data points is scattered and ***,there is no obvious relationship between the rock quality designation(RQD)and *** maximum horizontal stress σ_(H) is a function of rock properties,showing a certain linear relationship with the C_(ERP)at the same *** addition,the overall change trend of σ_(H) determined by the established fuzzy identification method is to increase with the increase of C_(ERP).The fuzzy identification method also demonstrates a relatively detailed local relationship betweenσ_H and C_(ERP),and the predicted curve rises in a fluctuating way,which is in accord well with the measured stress data.

关键词： overcoring stress measurements elastic modulus Poisson's ratio rock quality designation maximum horizontal stress fuzzy identification

Serum IgG response to differentiated antigens of Helicobacter pylori

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World Journal of Gastroenterology 1998年第3期4卷 69-71页

作者： HUA Jie Song 1, KHIN Mar Mar 1, zheng peng yuan 1, YEOH Khay Guan 2, Ng Han Chong 1 and HO Bow 1

AIM To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera. METHODS Blood samples were collected from 278 patients with gastric diseases. A 3 day old culture of H. pylori on chocolate blood agar was used to provide spiral form. ‘Synchronous’ coccoids were cultured in (BHY) (brain heart infusion supplemented with 10% horse serum and 0 4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori . RESULTS Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non ulcer dyspepsia (NUD) (44%) and other diseases (45%) ( P <0 05). IgG antibodies against spiral and coccoid antigens were detected in 50 7% (141/278) and 49 6% (138/278) , respectively. CONCLUSION The spiral and coccoid forms of H. pylori coexist in patients infected with the bacterium.

关键词： Helicobacter pylori spiral form coccoid form ELISA Helicobacter pylori infection Helicobacter pylori antigens, bacterial IgG antigens, differentiation antigen antibody reactions stomach ulcer stomach neoplasms gastritis

Peripheral mitochondrial DNA as a neuroinflammatory biomarker for major depressive disorder

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Neural Regeneration Research 2025年第6期20卷 1541-1554页

作者： Jinmei Ye Cong Duan Jiaxin Han Jinrong Chen Ning Sun yuan Li Tifei yuan Daihui peng Division of Mood Disorder Shanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Department of Psychiatry First Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Shanghai Key Laboratory of Psychotic Disorders Brain Health InstituteNational Center for Mental DisordersShanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Co-Innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina

In the pathogenesis of major depressive disorder, chronic stress-related neuroinflammation hinders favorable prognosis and antidepressant response. Mitochondrial DNA may be an inflammatory trigger, after its release from stress-induced dysfunctional central nervous system mitochondria into peripheral circulation. This evidence supports the potential use of peripheral mitochondrial DNA as a neuroinflammatory biomarker for the diagnosis and treatment of major depressive disorder. Herein, we critically review the neuroinflammation theory in major depressive disorder, providing compelling evidence that mitochondrial DNA release acts as a critical biological substrate, and that it constitutes the neuroinflammatory disease pathway. After its release, mitochondrial DNA can be carried in the exosomes and transported to extracellular spaces in the central nervous system and peripheral circulation. Detectable exosomes render encaged mitochondrial DNA relatively stable. This mitochondrial DNA in peripheral circulation can thus be directly detected in clinical practice. These characteristics illustrate the potential for mitochondrial DNA to serve as an innovative clinical biomarker and molecular treatment target for major depressive disorder. This review also highlights the future potential value of clinical applications combining mitochondrial DNA with a panel of other biomarkers, to improve diagnostic precision in major depressive disorder.

关键词： biomarker cytokine exosomes inflammasome major depressive disorder microglia mitochondrial DNA mitochondrial dysfunction neuroinflammation Toll-like receptor

具有结构不确定性的时滞系统的闭环鲁棒预测控制器(英文)

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控制理论与应用 2013年第6期30卷 683-692页

作者：郑鹏远席裕庚李德伟上海交通大学自动化系上海200240 系统控制与信息处理教育部重点实验室上海200240 上海交通大学密西根学院上海200240

针对具有结构不确定性的时滞系统,设计了闭环鲁棒预测控制算法.该控制算法基于控制不变集方法,通过采用双模控制和闭环控制策略,增加了控制设计的自由度,进而扩大了系统的初始可行域并能获得较优的控制性能.仿真结果验证了该方法的有效性.

关键词：鲁棒预测控制时滞结构不确定性双模控制控制不变集线性矩阵不等式

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微生态调节剂在抗生素相关性腹泻中的应用

中国实用内科杂志 2016年第9期36卷 750-753页

作者：梅璐郑鹏远郑州大学第五附属医院消化内科河南郑州450052

由于抗生素在人群中的广泛应用,抗生素相关性腹泻(AAD)成为许多患者常见的问题。目前,微生态调节剂已经广泛应用于AAD、艰难梭菌感染相关腹泻等疾病的治疗中。然而,针对不同的临床情况,微生态调节剂应用亦有其特点。该文主要从微生态调... 详细信息

由于抗生素在人群中的广泛应用,抗生素相关性腹泻(AAD)成为许多患者常见的问题。目前,微生态调节剂已经广泛应用于AAD、艰难梭菌感染相关腹泻等疾病的治疗中。然而,针对不同的临床情况,微生态调节剂应用亦有其特点。该文主要从微生态调节剂的选取、临床适应证以及使用剂量等方面阐述微生态调节剂在AAD中的应用。

关键词：微生态调节剂抗生素相关性腹泻肠道菌群失调