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The Crop Journal 2022年第2期10卷 368-374页

作者： Huanan Han Ziwen Wu ling zheng Jingyi Han Yi Zhang Jihu Li Shujuan Zhang Genying Li Changle Ma Pingping Wang College of Life Sciences Shandong Normal UniversityWenhua East Road 88Jinan 250014ShandongChina Crop Research Institute Shandong Academy of Agricultural SciencesJinan 250100ShandongChina

Base editing using CRISPR technologies is an invaluable tool for crop breeding. One of the major base editors, the adenine base editor(ABE), has been successfully used in both model plants and many ***, owing to limited editing efficiency, the ABE has been difficult to apply in polyploid crops such as allohexaploid bread wheat that often require simultaneous mutation of multiple alleles for fast breeding. We have designed a wheat high-efficiency ABE(Whie ABE), using the newly developed high-activity adenosine deaminase Tad A8 e. In vivo and in vitro analysis demonstrated the improved applicability of Tad A8 e over the commonly used Tad A7.10. Dinitroaniline is a widely used herbicide with high effectiveness and low toxicity to animals. However, wheat cultivars with tolerance to dinitroaniline are rare, limiting the application of dinitroaniline in wheat planting. Using A-to-G editing with Whie ABE, we found that a Met-to-Thr mutation in wheat tubulin alleles located on chromosomes 1 A, 1 B, 1 D, 4 A, and 4 D increased the resistance of wheat to dinitroaniline, revealing a dosage effect of edited tubulins in resistance. The Whie ABE promises to be a valuable editing tool for accelerating crop improvement and developing herbicide-resistant wheat germplasm.

关键词： CRISPR Base editing Dinitroaniline Herbicide-resistant Wheat breeding

Alcohol Dehydrogenase Activity in Cultured Cells from Different Tobacco (Nicotiana tabacum L.) Varieties

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Wuhan University Journal of Natural Sciences 1999年第4期4卷 495-497页

作者： zheng ling, YANG Yue sheng ,zheng Ying dong College of Biotechnology, South China Agricultural University, Guangzhou 510642,China College of Biotechnology South China Agricultural University Guanzhou China

The activity of alcohol dehydrogenase (ADH) in cultured cells of various tobacco was determined. It was found that significant differences existed in cells of different varieties cultured under normal conditions and as well after treated with exogenous ethanol. The ADH activity had positive relation with the ability of the cells to catabolize exogenous ethanol, indicating that the main function of the ADH in tobacco cells was in the direction of converting ethanol to acetaldehyde.

关键词： alcohol dehydrogenase suspension cultured cells tobacco

Isotypic heterojunction based on Fe-doped and terephthalaldehyde-modified carbon nitride for improving photocatalytic degradation with simultaneous hydrogen production

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Chinese Chemical Letters 2021年第9期32卷 2782-2786页

作者： Xun-Heng Jiang Fan Yu Dai-She Wu Lei Tian ling-ling zheng Li-Sha Chen Peng Chen Long-Shuai Zhang Hui Zeng Ying Chen Jian-Ping Zou Key Laboratory of Poyang Lake Environment and Resource Utilization of Ministry of Education School of Resources Environmental and Chemical EngineeringNanchang UniversityNanchang 330031China Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle Nanchang Hangkong UniversityNanchang 330063China National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization Nanchang Hangkong UniversityNanchang 330063China

To achieve an efficient photocatalytic for clean energy production and environmental remediation,the highly active Fe-doped and terephthalaldehyde-modified carbon nitride(Fe-CN/NTE)isotypic heterojunction photocatalyst is constructed via a simple annealing method for degradation of organic pollutants with simultaneous resource *** Fe-CN/NTE catalyst exhibits a 93%removal rate of pnitrophenol(4-NP)and a 1.72 mmol/g H2 evolution rate in 2 h simultaneously under visible light irradiation,which are higher than those of pristine CN,Fe-CN,and NTE,*** tests show that the excellent photocatalytic performance of Fe-CN/NTE comes from the improved migration,transportation,and separation of photoinduced charge carriers and expanded light-harvesting ***,hydroxyl radical(·OH),electron(e^(-)),and hole(h^(+))are the main active species and the rational mechanism of 4-NP photodegradation was proposed based on scavenger measurements and liquid chromatography-mass spectrometry(LC–MS),*** heterojunction Fe-CN/NTE photocatalyst possesses excellent stability in the H2 evolution and 4-NP degradation during five-run cycle tests,posing as a promising candidate in practical works for organic pollution and energy challenges.

关键词： Isotypic heterojunction Photocatalytic degradation Simultaneous hydrogen evolution Carbon nitride

Effects and mechanisms of Helicobacter pylori infection on the occurrence of extra-gastric tumors

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World Journal of Gastroenterology 2024年第37期30卷 4090-4103页

作者： Shi-Qing Zhao Hui-ling zheng Xiao-Tian Zhong Zi-Ye Wang Yi Su Yan-Yan Shi Research Center of Clinical Epidemiology Peking University Third HospitalBeijing 100191China Health Science Center Peking UniversityBeijing 100191China Department of Gastroenterology Peking University Third HospitalBeijing 100191China

Helicobacter pylori(***)colonizes the human stomach and many studies have discussed the mechanisms of *** infection leading to gastric diseases,including gastric ***,increasing data have shown that the infection of *** may contribute to the development of extra-gastric diseases and ***,systemic immune responses,microbiome disorders,and hypergastrinemia caused by *** infection are associated with many extra-gastric *** review highlights recent discoveries;discusses the relationship between *** and various extra-gastric tumors,such as colorectal cancer,lung cancer,cholangiocarcinoma,and gallbladder carcinoma;and explores the mechanisms of extra-gastric carcinogenesis by ***,these findings refine our understanding of the pathogenic processes of ***,provide guidance for the clinical treatment and management of ***-related extra-gastric tumors,and help improve prognosis.

关键词： Helicobacter pylori Extra-gastric tumors Immune system response Mucosal barrier Gut microbiome

Multi Objective Optimization of Vehicle Crashworthiness Based on Combined Surrogate Models

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Multi Objective Optimization of Vehicle Crashworthiness Base...

Ethanol Metabolism in Calluses of Several Selected Plant Species on Two Typical Plant-Growth-Regulator Balanced Media

2016中国汽车工程学会年会暨展览会

作者： zheng ling Gao Yinan Li Yinong Zhan Zhenfei College of Automotive Engineering Chongqing University

ISBN: (纸本)9789811035272;9789811035265

Several surrogate models such as radial basis function and Kriging models are developed to speed the optimization design of vehicle body and improve the vehicle *** error analysis is used to investigate the accuracy of different surrogate ***,the Kriging model is used to fit the model of B-pillar acceleration and foot well *** multiquadric radial basis function is used to fit the model of the entire vehicle *** models are further used to calculate the acceleration response in B-pillar,foot well intrusion and vehicle mass instead of the finite element model in the optimization design of vehicle crashworthiness.A multi-objective optimization problem is formulated in order to improve vehicle safety performance and keep its light *** particle swarm method is used to solve the proposed multi-objective optimization *** simulation results show that the B-pillar acceleration and the foot well intrusion are reduced 4.9%,6.31% *** entire vehicle mass is reduced *** proposed combined surrogate models and their applications in the optimization design of vehicle crashworthiness provide an important design method.

关键词： vehicle crashworthiness radial basis function Kriging model particle swarm

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Wuhan University Journal of Natural Sciences 2000年第4期5卷 500-504页

作者： Yang Yue-sheng, zheng Ying-dong, Chen Yu-fen, zheng ling College of Biotechnology, South China Agricultural University, Guangzhou 510642, China College of Biotechnology South China Agricultural University Guangzhou China

For investigation on the characteristics of ethanol metabolism in tissues of different plant species, calluses from eight selected plant species were cultured on medium supplemented with ethanol in tightly sealed culture flasks. Changes of the ethanol level were detected by gas chromatography. During the culture period, the calluses of tobacco, potato and petunia were, able to catabolize exogenous ethanol, resulting in the prominent decline of the ethanol level in the medium. The calluses of melon and peanut were also able to catabolize thanol but with lower efficiency. The other three calluses of carrot, soybean and rice did not catabolize ethanol but instead produced small to large amount of ethanol, resulting in the increase of the ethanol level in the media. It was also found that changing the balance between auxin and cytokinin could influence only the ethanol metabolism efficiency but could not change the metabolism patterns on ethanol of the cultured calluses. It can be concluded that, ethanol metabolism pattern of calluses in cultures is an innate physiological characteristic of the respective plant species.

关键词： callus culture ethanol metabolism plant growth regulator plant physiology

Impact of warm mesoscale eddy on tropical cyclone intensity

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Acta Oceanologica Sinica 2020年第8期39卷 1-13页

作者： Jia Sun Guihua Wang Xuejun Xiong Zhenli Hui Xiaomin Hu zheng ling Long Yu Guangbing Yang Yanliang Guo Xia Ju Liang Chen First Institute of Oceanography Ministry of Natural ResourcesQingdao 266061China Laboratory for Regional Oceanography and Numerical Modeling Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Institute of Atmospheric Sciences Department of Environmental Science and EngineeringFudan UniversityShanghai 200433China Guangdong Key Laboratory of Coastal Ocean Variability and Disaster Prediction Guangdong Ocean UniversityZhanjiang 524088China College of Oceanic and Atmospheric Sciences Ocean University of ChinaQingdao 266100China

The spatial-temporal patterns of tropical cyclone(TC) intensity changes caused by the warm ocean mesoscale eddy(WOME) distribution are evaluated using two sets of idealized numerical experiments. The results show that the TC was intensified and weakened when a WOME was close to and far away from the TC center, *** area where the WOME enhanced(weakened) TC intensity is called the inner(outer) area in this *** of the enhancement and weakening caused by the WOME in the inner and outer area decreased and increased over time, while the ranges of the inner and outer area diminished and expanded, respectively. The WOME in the inner area strengthened the secondary circulation of the TC, increased heat fluxes, strengthened the symmetry, and weakened the outer spiral rainband, which enhanced TC intensity. The effect was opposite if the WOME was in the outer area, and it weakened the TC intensity. The idealized simulation employed a stationary TC, and thus the results may only be applied to TCs with slow propagation. These findings can improve our understanding of the interactions between TC and the WOME and are helpful for improving TC intensity forecasting by considering the effect of the WOME in the outer areas.

关键词： tropical cyclone intensity warm ocean mesoscale eddy upper ocean spatial-temporal pattern

基于磁流变减振器的汽车半主动悬架非线性控制方法

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机械工程学报 2005年第5期41卷 31-37页

作者：李以农郑玲重庆大学机械传动国家重点实验室重庆400044

考虑磁流变减振器阻尼力和悬架弹性元件非线性特性,建立车辆半主动悬架非线性动力学模型。应用微分几何非线性控制,经过适当的非线性状态和反馈变换,实现半主动悬架非线性系统的精确线性化,并对系统实施非线性状态反馈控制;根据预定的... 详细信息

考虑磁流变减振器阻尼力和悬架弹性元件非线性特性,建立车辆半主动悬架非线性动力学模型。应用微分几何非线性控制,经过适当的非线性状态和反馈变换,实现半主动悬架非线性系统的精确线性化,并对系统实施非线性状态反馈控制;根据预定的控制目标及模糊控制策略调节控制参数,设计模糊控制器,对悬架系统进行了控制仿真研究;利用神经网络模式识别能力对输入数据处理辨别,设计控制网络层,从而达到提高悬架工作性能,改善汽车行驶舒适性的目的。将三种非线性控制方法的仿真结果进行分析比较表明:经模糊控制或神经网络控制后的悬架承受的冲击响应小、振动强度低,比微分几何控制能获得更优异的性能。

关键词：半主动悬架非线性控制磁流变减振器微分几何模糊神经网络

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车辆半主动悬架非线性控制方法的研究

农业机械学报 2005年第5期36卷 9-15页

作者：李以农郑玲重庆大学机械传动国家重点实验室

考虑磁流变减振器阻尼力和悬架弹性元件非线性特性,建立了车辆半主动悬架非线性动力学模型。应用微分几何非线性控制,经过适当的非线性状态和反馈变换,实现半主动悬架非线性系统的精确线性化,并对系统实施非线性状态反馈控制;根据预定... 详细信息

考虑磁流变减振器阻尼力和悬架弹性元件非线性特性,建立了车辆半主动悬架非线性动力学模型。应用微分几何非线性控制,经过适当的非线性状态和反馈变换,实现半主动悬架非线性系统的精确线性化,并对系统实施非线性状态反馈控制;根据预定的控制目标及模糊控制策略调节控制参数,设计模糊控制器,对悬架系统进行了控制仿真研究;利用神经网络模式识别能力对输入数据处理辨别,设计控制网络层,从而达到提高悬架工作性能,改善车辆行驶舒适性的目的。将3种非线性控制方法的仿真结果进行分析比较表明:经模糊控制或神经网络控制的悬架冲击响应小、振动强度低,比微分几何控制能获得更优异的性能。

关键词：车辆半主动悬架非线性控制模糊控制神经网络控制

Transforming growth factor-β1 in invasion and metastasis in colorectal cancer

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World Journal of Gastroenterology 2002年第4期8卷 674-678页

作者： Bin Xiong Hong-Yin Yuan Ming-Bo Hu Feng Zhang zheng-Zhuan Wei ling-ling Gong Guo-Liang Yang Department of Oncology,Affiliated Zhongnan Hospital of Wuhan University,Wuhan 430071,Hubei Province,China Department of Oncology Affiliated Zhongnan Hospital of Wuhan UniversityWuhan 430071Hubei ProvinceChina. xbxh@***

AIM：To investigate the role of TGFβ1 in invasion and metastasis in colorectal cancer by analysing TGFβ1 correlated wity depth of tumor invasion,stage and ***:Serum TGFβ1levels were determined in50patients with colorectal cancer and 30healthy volunteers using a TGFβ1 enzyme-linked immunosorbent ***β1 expression in primary and lymph node metastatic lesions were detected in 98cases of colorectal cancer by immunohistochemical staining and in situ ***:Serum levels of TGFβ1 in patients with colorectal cancer(40±18μg·L^-1)were significantly higher than those in the healthy control group(19±8μg·L^-1),P<*** levels of serum TGFβ1were found in 60%of patients with colorectal cancer when the mean+2s was used as the upper limit of the normal range(35.1μg·L^-1).Increases in serum TGFβ1 levels were significantly asociated with Dukei's stage(P<0.05),but there was no significant difference between,Duki's stage Bpatients and Dukei's stage *** the cytoplasm of cancer cells,TGFβ1 was immunostained in37.8%(37/98)of colorectal cancer,and this expression was confirmed by in situ hybridization,Among35cases of colorectal cancer with lymph node metastatic lesions,TGFβ1 positive staining was found in18(51.4%)cases of primary tumor,and 25(71.4%)cases with lymph node metastatic lesions,respectively,Of17cases with no staining in the primary lesion.7(41.2%)casesshowed TGFβ1 staining in the metastatic *** TGFβ1 levels and TGFβ1 expression in colorectal cancer tissues were correlated significantly with depth of tumor invasion,stage and metastasis,Patients in stage C-D,T3-T4and with metastasis had significantly higher TGFβ1 levels than patients in stage A-B,T1-T2and without metastasis(P<0.05).CONCLUSION:These results suggest that transforming growth factor-β1 is closely related to the invasion and metastasis of colorectal *** increased the invasive and metastatic potential of tumor by altering a tumor ***β1 ma

关键词：结直肠癌转化生长因子β1 肿瘤侵袭肿瘤转移作用机制