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Endopeptidase Isoenzyme Characteristics in Cucumis sativus Leaves During Dark-induced Senescence

Journal of Integrative Plant Biology 2007年第4期49卷 507-514页

作者： Peng zhang Fei Wang Lie-Feng zhang qi rui Lang-Lai Xu College of Life Sciences Nanjing Agricultural University Nanjing 210095 China

The changes and characteristics of endopeptidase （EP） isoenzymes in cucumber （Cucumis sativus L.） leaves during dark-induced senescence were investigated by activity staining after gradient-polyacrylamide gel electrophoresis （G-PAGE） containing co-polymerized gelatin as substrate. The results showed that both the chlorophyll and the protein contents of leaves were decreased, and the protein degradation was correlated with the increase of proteolytic activity during the course of leaf senescence. Meanwhile, nine cucumber endopeptidases isoenzymes （CEP） with 140, 120, 106, 94, 76, 55, 46, 39 and 35 kDa molecular weights were detected. Four of these, CEP2, 3, 4 and CEP9 appeared all the time, but the changes of the activity were different during incubation. Another four CEPs （CEP5, 6, 7 and CEP8） whose activities increased with dark-induced time were only detected in senescent leaves. Furthermore, the biochemical properties of these nine CEP were also characterized. All the CEPs had high activities from 35 ℃ to 45 ℃, and the optimum temperature was found to be 40 ℃. However, the activities of CEPs were not detected below 25 ℃ or over 60 ℃. The activity bands appeared at a wide range of pH from 5.0 to 9.0, but the optimum pH was found at 7.0. No CEPs were detected at pH 4 or pH 10. By inhibition analysis we concluded that CEP2, 3, 4 and CEP9 were serine endopeptidases and CEP6 was a kind of cysteine protease. It is suggested that serine endopeptidases might play a major role in cucumber leaf senescence, and for the first time, six senescencerelated endopeptidases （CEP1, 5, 6, 7, 8 and 9） were found in cucumber leaves.

关键词： Cucumis sativus cucumber endopeptidase leaf senescence.

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Structural and Critical Behaviors of Ag Rough Films Deposited on Liquid Substrates

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Chinese Physics Letters 1996年第10期13卷 772-774页

作者： YE Gao-xiang FENG Chun-mu zhang qi-rui GE Hong-liang zhang Xuan-jia Department of Physics Hangzhou UniversityHangzhou 310028 Department of Physics Zhejiang UniversityHangzhou 310027

A new Ag rough film system, deposited on silicone oil surfaces by rf-magnetron sputtering method, has been fabricated. The chrysanthemum-like surface morphology at micron length scale is observed. It is proposed that the anomalous critical behavior mainly results from the relative shift between the Ag atom clusters and the substrate. The discussion of the deposition mechanism is also presented.

关键词： Rough mechanism film

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Formation of Large Bipolarons

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Chinese Physics Letters 1997年第8期14卷 601-604页

作者： CHEN qing-hu REN Yu-hang JIAO Zheng-kuan zhang qi-rui Department of Physics Zhejiang UniversityHangzhou 310027

A variational approach is presented to calculate the ground-state energy of large bipolarons in two and three *** values of the critical coupling constantαc above which the bipolaron is stable and the critical ratio of dielectric constantsηc below which the bipolaron is stable are approximately *** is found that the stability region of bipolarons in the(l/α,η)-plane becomes larger with decreasing dimensionality.

关键词： critical dimensionality constants

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New anti-pulmonary fibrosis prenylflavonoid glycosides from Epimedium Koreanuin

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Chinese Journal of Natural Medicines 2022年第3期20卷 221-228页

作者： ZHAO Yu-Dan zhang Xin YANG Wan-Yue zhang rui-qi MU Lin-Tong HAN Ling LV Chong-Ning LU Jin-Cai School of Traditional Chinese Materia Medica Shenyang Pharmaceutical UniversityShenyang 110016China Liaoning Provincial Key Laboratory of TCM Resources Conservation and Development Shenyang Pharmaceutical UniversityShenyang 110016China NERCfor the Pharmaceutics of Traditional Chinese Medicines Benxi 117004China

Four new prenylflavonoid glycosides,namely koreanoside H-K(1-4),together with eleven known ones(5-15)were isolated from the leaves of Epimedium koreanum *** structures were elucidated by ID NMR,2D NMR,HR-ESI-MS,IR and *** identification of the sugar moieties was carried out by means of acid hydrolysis and HPLC analysis of their *** is worth noting that compound 3 and compound 4 were elucidated to contain fucose and quinovose moieties,which were two extremely rare sugar units from the genus *** anti-pulmonary fibrosis activity of the new compounds was evaluated using A549 cell *** 1,2 and 4 showed significant anti-pulmonary fibrosis activities.

关键词： Epimedium koreanum Prenylflavonoid glycoside Anti-pulmonary fibrosis

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Agronomic characterization and genetic analysis of the supernume-r ary spikelet in tetraploid wheat(Triticum turgidum L.)

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Journal of Integrative Agriculture 2017年第6期16卷 1304-1311页

作者： zhang rui-qi HOU Fu CHEN Juan CHEN Shu-lin XING Li-ping FENG Yi-gao CAO Ai-zhong College of Agronomy Nanjing Agricultural University/National Key Laboratory of Crop Genetics and Germplasm Enhancement/Cytogenetic Institute Jangsu Collaborative Innovation Center of Modern Crop Production College of Agronomy Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops

The supernumerary spikelets（SS）characters of tetraploid wheat（Triticum turgidum L.）resulting in more spikelets and kernels per spike,thus enhancing sink capacity may contribute to potential wheat yield *** order to investigate the effect of different SS types on agronomic characters and understand the genetic base of SS phenotype in tetraploid wheat,near isogenic lines（NILs）,bh-50 with normal spikelets（NS）,bh-51 with four-rowed spikelets（FRS）,bh-52 with short-ramified spikelets（SRS）,and bh-53 with long-ramified spikelets（LRS）in a Triticum durum ***1286 genetic background were developed by continuous *** characters showed that the SS phenotype lines,bh-51,bh-52and bh-53 have significant increase in the number of spikelets and grains per spike compared with the NS phenotype line bh-50（P〈0.05）,and bh-53 line showed much more increase than those of bh-51 and ***,bh-53 had the lowest grain weight and the longest spike development stage than those of other spike *** results indicated that the different SS types have different effects on the agronomic and spike *** analysis through bh-50/bh-51and bh-51/bh-53 F2 populations showed that a recessive major gene controlled the spike architecture to transform from NS to FRS,and a dominant major gene determined the change of spike phenotype from FRS to *** sequences of Tt BH/WFZP ortholog on chromosome 2AS revealed that a single nucleotide polymorphism（SNP）substitution happened in the open reading frame（ORF）region of all the SS tetraploid wheat accessions,which may lead to the generation of lateral meristems between glume and lemma during the immature spike *** the fates of the lateral meristems,developing into lateral spikelets or branched spikelets,may be determined by another major *** results presented here may advance our understanding and knowledge of the genes and genetic pathways determining the spike architecture development in wh

关键词： tetraploid wheat (Trificum turgidum L.) ramified spikelet four-rowed spikelet TtBH/WFZP alleles

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CFD study of flow-diffusion process in Y-shape micromixer

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Journal of Central South University 2016年第4期23卷 969-974页

作者：陈卓张睿琦王晓娜 School of Energy Science and Engineering Central South University

A CFD simulation was carried out to investigate the mixing process in a Y-shape micromixer with the software Fluent 6.3. The definition of the "diffusion angle" is proposed to describe the molecular diffusion process associated with the flow at low Reynolds number. The linear relationship between the diffusion angle and the Peclet number(Pe) is determined by both theoretical analysis and numerical simulation. Moreover, the simulation results reveal that the diffusion angle is only related to the Peclet number whilst it is irrelevant to the changes of Re(Reynolds number) and Sc(Schmidt number). The range of Peclet number and Reynolds number for experimental measurement are also suggested as Pe≤10000 and Re≤10.

关键词： simulation flow-diffusion process molecular diffusion Y-shape micromixer

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Relationship between Toll-like receptor 2 on polymorphonuclear neutrophil and postoperative systemic inflammatory response syndrome in patients undergoing orthotopic liver transplantation

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中华麻醉学杂志 2010年第B2期30卷 15-18页

作者： CHI Xin-jin LI Shang-rong CHENG Nan HEI Zi-qing LUO Gang-jian GUAN Jian-qiang zhang rui zhang qi Department of Anesthesiology Third Affiliated Hospital SUN Yat-Sen University Guangzhou 510630 China

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一种循环保护前缀不足的OFDM信道估计算法

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电波科学学报 2005年第5期20卷 632-636页

作者：张瑞齐廖桂生西安电子科技大学雷达信号处理国家重点实验室陕西西安710071

在正交频分复用(orthogonalfrequencydivisionmultiplexing,OFDM)通信系统中,信道估计占有很重要的地位。论文提出了一种在OFDM信号循环保护前缀不足情况下也可应用的盲信道估计算法,它利用循环前缀信号结构采用子空间分解,计算简单。... 详细信息

在正交频分复用(orthogonalfrequencydivisionmultiplexing,OFDM)通信系统中,信道估计占有很重要的地位。论文提出了一种在OFDM信号循环保护前缀不足情况下也可应用的盲信道估计算法,它利用循环前缀信号结构采用子空间分解,计算简单。计算机仿真显示其有良好的性能。

关键词： OFDM 信道估计循环前缀(CP)

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Oxidation State of Cu and Superconductivity in Bilayer T1-Based Cuprates

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Chinese Physics Letters 1997年第3期14卷 221-224页

作者： CHEN Xiao-jia XU Zhu-an JIAO Zheng-kuan zhang qi-rui Department of Physics Zhejiang UniversityHangzhou 310027

The oxidation state of Cu in the homologous Tl_(2)Ba_(2)Ca_(n-1)Cu_(n)O_(2n+4+δ)(n=1-4)series has been analyzed in terms of the bond valence *** is found that the totally mobile hole concentration nH,t of the type-Ⅰ CuO_(2) planes at the optimal level increases with increasing number of CuO_(2) layers per unit cell from 1 to 3,and the largest nH,t is observed to be 0.474 when n=3,which just corresponds to the highest T_(c) in the bilayer T1-based materials,and then it decreases with the increase of *** is indicated that the correlation between the maximum value of T_(c) and n is similar to that between n_(H,t) and n.

关键词： totally optimal homologous

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Low-frequency swell noise suppression based on U-Net

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Applied Geophysics 2020年第3期17卷 419-431页

作者： zhang rui-qi Song Peng Liu Bao-hua zhang Xiao-bo Tan Jun Zou Zhi-hui Xie Chuang Wang Shao-wen College of Marine Geosciences Ocean University of ChinaQingdao 266100China. Laboratory for MMR Qingdao National Laboratory for Marine Science and TechnologyQingdao 266100China. Key Laboratory of Submarine Geoscience and Prospecting Techniques Ministry of EducationQingdao 266100China National Deep Sea Center Qingdao 266237China.

Low-frequency band-shaped swell noise with strong amplitude is common in marine seismic *** conventional high-pass fi ltering algorithm widely used to suppress swell noise often results in serious damage of effective *** paper introduces the residual learning strategy of denoising convolutional neural network(DnCNN)into a U-shaped convolutional neural network(U-Net)to develop a new U-Net with more generalization,which can eliminate low-frequency swell noise with high *** results of both model date tests and real data processing show that the new U-Net is capable of effi cient learning and high-precision noise removal,and can avoid the overfi tting problem which is very common in conventional neural network *** new U-Net can also be generalized to some extent and can eff ectively preserve low-frequency eff ective *** with the conventional high-pass fi ltering method commonly used in the industry,the new U-Net can eliminate low-frequency swell noise with higher precision while eff ectively preserving low-frequency eff ective information,which is of great signifi cance for subsequent processing such as amplitude-preserving imaging and full waveform inversion.

关键词： U-Net swell noise noise attenuation residual learning generalization

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