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A 9.5-kb deletion in the 1st intron of OsMADS51 enhances temperature sensitivity in rice

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The Crop Journal 2024年第4期12卷 1031-1040页

作者： Lei zhao Haifei Hu Junyu Chen Chongrong Wang Yibo Chen Hong Li Daoqiang Huang Zhidong Wang Degui Zhou Rong Gong Yangyang Pan Junliang zhao Liangyong Ma Shaochuan Zhou Rice Research institute Guangdong Academy of Agricultural SciencesGuangzhou 510640GuangdongChina State Key Laboratory of Rice Biology and chinese National Center for Rice Improvement China National Rice Research InstituteHangzhou 310006ZhejiangChina Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510640GuangdongChina Key Laboratory of Genetics and Breeding of High Quality Rice in Southern china(Co-construction by Ministry and Province) Ministry of Agriculture and Rural AffairsGuangzhou 510640GuangdongChina Guangdong Rice Engineering Laboratory Guangzhou 510640GuangdongChina Guangdong Key Laboratory of New Technology in Rice Breeding Guangzhou 510640GuangdongChina

Temperature is an important environmental factor affecting heading date of *** its importance,genes responsible for temperature-sensitive heading in rice have remained *** previous study identified a quantitative trait locus qHd1 which advances heading date under high temperatures.A 9.5-kb insertion was found in the first intron of OsMADS51 in indica variety Zhenshan 97(ZS97).However,the function of this natural variant in controlling temperature sensitivity has not been *** this study,we used CRISPR/Cas9 to knock out the 9.5-kb insertion in *** conducted under cotrolled conditions in phytotrons confirmed that deletion increased temperature sensitivity and advanced heading by downregulating the expression level of ***-hybrid assays in yeast,ChIP-quantitative polymerase chain reaction,electrophoretic mobility shift,and luciferase-based transient transactivation assays collectively confirmed that OsMADS51 affects heading date by regulation of heading date gene *** further determined that the long non-coding RNA HEATINR is generated from the first intron of OsMADS51,offering an explanation for how the 9.5-kb insertion affects temperature *** also found that OsMADS51 was strongly selected in early/late-season rice varieties in South china,possibly accounting for their strong temperature *** insights not only advance our understanding of the molecular mechanisms underlying the temperature-responsive regulation of heading date in rice but also provide a valuable genetic target for molecular breeding.

关键词： OsMADS51 Insertion Long non-coding RNA Oryza sativa

Excitation energy transfer mechanisms in allophycocyanin(Ⅰ)——Energy transfer mechanisms in monomer

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chinese Science Bulletin 1999年第11期44卷 1003-1008页

作者： ZHANG Jingmin, ZHENG Xiguang, zhao Fuli, WANG Hezhou, ZHANG Jianping, zhao Jingquan and JIANG Lijin State Key Laboratory for Ultrafast Laser Spectroscopy, Zhongshan University, {2. 5102.5, china institute of Photographic Chemistry , chinese academy of sciences , Beijing 100101, china

The mechanisms of excitation energy transfer within allophycocyanin monomer with the theory of generalized master equation (GME) and the technique of time-resolved fluorescence anisotropic spectroscopy are studied. In the case of known information of its structure and spectra, the theory applied is based on the assumption that the coupling interaction between two chromophores is fairly weak. The theory correctly predicts the experimentlly observed rate for excitation energy transfer in allophycocyanin monomer. Based on the results, the energy transfer mechanism can be described as Frster and these processes cannot take place from the high vibrational levels of donor to acceptor.

关键词： allophycocyanin energy transfer generalized master equation time-resolved fluorescence anisotropic spec-troscopy.

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生物质富氧—水蒸气气化制氢特性研究

太阳能学报 2006年第7期27卷 677-681页

作者：赵先国常杰吕鹏梅王铁军付严中国科学院广州能源研究所

以一个鼓泡流化床为反应器，对生物质富氧-水蒸气气化制取富氢燃气的特性进行了一系列的实验研究。通过对试验数据的分析，探讨了主要参数温度、水蒸气，生物质（S/B）和氧浓度对气体成分、氢产率和潜在产氢量的影响。结果表明：在3个... 详细信息

以一个鼓泡流化床为反应器，对生物质富氧-水蒸气气化制取富氢燃气的特性进行了一系列的实验研究。通过对试验数据的分析，探讨了主要参数温度、水蒸气，生物质（S/B）和氧浓度对气体成分、氢产率和潜在产氢量的影响。结果表明：在3个主要参数的变化范围内，氢产率和潜在氢产量受温度的影响最大：当温度从700～900℃时，每千克生物质氢产量从18g增加到了53g，每千克生物质潜在氢产量从71．6g增加到了115.6g。

关键词：生物质富氧水蒸气气化制氢

Spatial Distribution of Heavy Metals in Agricultural Soils of an Industry-Based Peri-Urban Area in Wuxi, china

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Pedosphere 2007年第1期17卷 44-51页

作者： zhao Yan-Feng SHI Xue-Zheng HUANG Biao YU Dong-Sheng WANG Hong-Jie SUN Wei-Xia I. OEBOERN K. BLOMBACK State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China） Department of Soil Science the Swedish University of Agricultural Sciences SE-75007 Uppsala （Sweden） Graduate School of the chinese academy of {2. Beijing 100039 （China）

In industry-oriented peri-urban areas, the heavy metal accumulation in soils caused by industrialization has become a potential threat. The top soil samples from 2. paddy fields and 75 vegetable fields were collected from a typical industry- based peri-urban area of about 8 km^2.in Wuxi, china, to study the accumulation and distribution of As, Hg, Cu, Zn, Pb, Cr, and Cd in comparison with heavy metal contents in soils near developed industrial sites （guangzhou, china; Wallsend Burn of Tyneside, UK; and Osnabrück, Germany）. Kriging interpolation was used to determine the metals, spatial distribution. The results showed that most soils, compared to the background values, contained elevated contents of As, Hg, Cu, Zn, and Pb with some having elevated contents of Cd and Cr. Except for less than 10% of the soil samples of Cu, Zn and Cd contents, these heavy metal contents were lower than the soil threshold levels of the Grade Ⅱ criteria for the chinese environmental quality standard. Probably, because of the scattered distribution and diversity of industries in the study area, spatial distributions of these heavy metals from Kriging interpolation indicated little similarity. Nevertheless, when compared with other areas in the Taihu Lake region, mean contents of Cu, Zn, Pb, and Cd were relatively high in the Wuxi peri-urban area. Additionally, compared to soils in agricultural areas around guangzhou, Osnabrück, or Wallsend Burn, contents of most heavy metals in soils from this area were lower.

关键词： agricultural soil heavy metals peri-urban area spatial distribution

Astragalus Polysaccharide Enhances Voriconazole Metabolism under Inflammatory Conditions through the Gut Microbiota

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Journal of Clinical and Translational Hepatology 2024年第5期12卷 481-495页

作者： Xiaokang Wang Xianjing Hu Chunxiao Ye Jingqian zhao Shing Cheng Tan Liangbin Zhou Chenyu zhao Kit Hang Wu Xixiao Yang Jinbin Wei Maoxun Yang The Marine Biomedical Research institute of Guangdong Zhanjiang ZhanjiangGuangdongChina Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs Guangdong Medical UniversityDongguanGuangdongChina Department of Pharmacy Shenzhen Longhua District Central HospitalShenzhenGuangdongChina Dongguan Key Laboratory of Chronic Inflammatory Diseases The First Dongguan Affiliated HospitalGuangdong Medical UniversityDongguanGuangdongChina Department of Pharmacy The Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdongChina Department of Pharmacy Shenzhen Hospital of Southern Medical UniversityShenzhenGuangdongChina UKM Medical Molecular Biology institute Universiti Kebangsaan MalaysiaKuala LumpurMalaysia Department of Biomedical Engineering Faculty of EngineeringThe Chinese University of Hong KongHong Kong SARChina Department of Pharmacy Nossa Senhora do Carmo-Lago Health CentreHealth BureauMacaoChina Pharmaceutical College Guangxi Medical UniversityNanningGuangxiChina

Background and Aims:Voriconazole(VRC),a widely used antifungal drug,often causes hepatotoxicity,which presents a significant clinical *** studies demonstrated that Astragalus polysaccharide(APS)can regulate VRC metabolism,thereby potentially mitigating its hepatotoxic *** this study,we aimed to explore the mechanism by which APS regulates VRC ***:First,we assessed the association of abnormal VRC metabolism with hepatotoxicity using the Roussel Uclaf Causality Assessment Method ***,we conducted a series of basic experiments to verify the promotive effect of APS on VRC *** in vitro and in vivo assays,including cytokine profiling,immunohistochemistry,quantitative polymerase chain reaction,metabolite analysis,and drug concentration measurements,were performed using a lipopolysaccharideinduced rat inflammation ***,experiments such as intestinal biodiversity analysis,intestinal clearance assessments,and Bifidobacterium bifidum replenishment were performed to examine the ability of *** to regulate the expression of the VRC-metabolizing enzyme CYP2.19 through the gut–liver ***:The results indicated that APS does not have a direct effect on ***,the assessment of gut microbiota function revealed that APS significantly increases the abundance of ***,which could lead to an anti-inflammatory response in the liver and indirectly enhance VRC *** dual-luciferase reporter gene assay revealed that APS can hinder the secretion of pro-inflammatory mediators and reduce the inhibitory effect on CYP2.19 transcription through the nuclear factor-􀇉B signaling ***:The study offers valuable insights into the mechanism by which APS alleviates VRC-induced liver damage,highlighting its immunomodulatory influence on hepatic tissues and its indirect regulatory control of VRC-metabolizing enzymes within hepatocytes.

关键词： Astragalus polysaccharide Voriconazole Metabolism RUCAM Bifidobacterium bifidum Anti-inflammatory responses Gut Microbiota Hepatotoxicity

Spatial Characteristics of Soil Organic Carbon Storage in china's Croplands

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Pedosphere 2005年第4期15卷 417-423页

作者： WANG Shao-Qiang YU Gui-Rui zhao Qian-Jun NIU Dong CHEN Qing-Mei WU Zhi-Feng institute of Geographic sciences and Natural Resources Research Beijing 10010 （China）. Guangdong institute of Eco-Environment and Soil Science Guangzhou 510650 （China）

The soil organic carbon (SOC) pool is the largest component of terrestrial carbon pools. With the construction of a geographically referenced database taken from the second national general soil survey materials and based on 1546 typical cropland soil profiles, the paddy field and dryland SOC storage among six regions of china were systematically quantified to characterize the spatial pattern of cropland SOC storage in china and to examine the relationship between mean annual temperature, precipitation, soil texture features and SOC content. In all regions, paddy soils had higher SOC storage than dryland soils, and cropland SOC content was the highest in Southwest china. Climate controlled the spatial distribution of SOC in both paddy and dryland soils, with SOC storage increasing with increasing precipitation and decreasing with increasing temperature.

关键词： carbon storage cropland soil organic carbon spatial characteristics

OsDA1 positively regulates grain width in rice

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The Crop Journal 2024年第1期12卷 92-101页

作者： Cong Li Jun Liu Liya Zhang Tao Li Hongyu Li Bin Liu Tao zhao institute of Crop sciences Chinese Academy of Agricultural SciencesBeijing 100081China institute of Nanfan&Seed Industry Guangdong Academy of SciencesGuangzhou 510316GuangdongChina

The size and shape of rice grains influence their yield and commercial *** investigated the role of OsDA1,a rice homolog of the Arabidopsis DA1 gene,in regulating grain size and ***1 was highly expressed in young spikelets and *** overexpression led to enlarged seeds with increased width and decreased length/width ratio(LWR)and knocking out OsDA1 reduced grain width and increased grain length and LWR.A R310K point mutation in the DA1-like domain is a potential target for breeding for increased grain width and ***1 interacted with TCP gene-family proteins to regulate grain size and *** findings deepen our understanding of the molecular mechanisms underlying grain size regulation and provide useful information for improving grain yield.

关键词： Rice Osda1 Grain size Grain shape Grain length/width ratio TCP

A Review of Intelligent Configuration and Its Security for Complex Networks

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chinese Journal of Electronics 2024年第4期33卷 920-947页

作者： Yue zhao Bin YANG Fei TENG Xianhua NIU Ning HU Bo TIAN Science and Technology on Communication Security Laboratory School of Computer and Information Engineering Chuzhou University School of Computing and Artificial Intelligence Southwest Jiaotong University School of Computer and Software Engineering Xihua University Cyberspace institute of Advanced Technology Guangzhou University

Complex networks are becoming more complex because of the use of many components with diverse technologies. In fact, manual configuration that makes each component interoperable has breed latent danger to system security. There is still no comprehensive review of these studies and prospects for further research. According to the complexity of component configuration and difficulty of security assurance in typical complex networks, this paper systematically reviews the abstract models and formal analysis methods required for intelligent configuration of complex networks, specifically analyzes, and compares the current key technologies such as configuration semantic awareness, automatic generation of security configuration, dynamic deployment, and verification evaluation. These technologies can effectively improve the security of complex networks intelligent configuration and reduce the complexity of operation and maintenance. This paper also summarizes the mainstream construction methods of complex networks configuration and its security test environment and detection index system, which lays a theoretical foundation for the formation of the comprehensive effectiveness verification capability of configuration security. The whole lifecycle management system of configuration security process proposed in this paper provides an important technical reference for reducing the complexity of network operation and maintenance and improving network security.

关键词： Complex networks Intelligent configuration Configuration security Software defined network Lifecycle management SOFTWARE ALGORITHM AGENT

Environmental response to ore deposits and mining of the Diaojiang River basin, Guangxi (South china)

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Acta Geochimica 2006年第S1期 40-41页

作者： Yongzhang ZHOU1, Zhi DANG2. Xianzhi PENG3, Zhijun YANG1, Chengbo ZHANG1, Xiaoqiang YANG1, Shanming FU1, Yuyan zhao1 1 Center for Earth’s Environment and Resources, Zhongshan University, guangzhou 5102.5,{2. 2.School of Environment sciences, South{2. University of Technology, guangzhou 51064.,{2. 3 guangzhou institute of Geochemistry, chinese academy of sciences, guangzhou 51064.,{2.