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Neuroprotective and antidiabetic lanostane-type triterpenoids from the fruiting bodies of Ganoderma theaecolum

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Chinese Journal of Natural Medicines 2025年第2期23卷 245-256页

作者： Jiaocen Guo Li yang Luting Dai Qingyun Ma Jiaoyang yan Qingyi Xie Yougen Wu Haofu Dai Youxing zhao School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication) Hainan University Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province & National Key Laboratory for Tropical Crop Breeding Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences

Eight previously undescribed lanostane triterpenoids,including five nortriterpenoids with 2 6carbons,ganothenoids A-E（1-5）,and three lanostanoids,ganothenoids F-H（6-8）,along with 24 known ones（9-32）,were isolated from the fruiting bodies of Ganodrma *** structures of the novel compounds were elucidated using comprehensive spectroscopic methods,including electronic circular dichroism（ECD） and nuclear magnetic resonance（NMR） *** 1-32 were assessed for their neuroprotective effects against H2O2-induced damage in human neuroblastoma SH-SY5Y cells,as well as their inhibitory activities against protein tyrosine phosphatase 1B（PTP1B） and α-*** 4demonstrated the most potent neuroprotective activity against H2O2-induced oxidative stress by suppressing G0/G1phase cell cycle arrest,reducing reactive oxygen species（ROS） levels,and inhibiting cell apoptosis through modulation of B-cell lymphoma 2 protein（Bcl-2） and Bcl-2 associated X-protein（Bax） protein *** 26,12,and 28 exhibited PTP1B inhibitory activities with IC50values ranging from 13.92 to 56.94 μmol·L-1,while compound12 alone displayed significant inhibitory effects on α-glucosidase with an IC50value of 43.56μmol·***,enzyme kinetic analyses and molecular docking simulations were conducted for compounds 26 and 12 with PTP1B and α-glucosidase,respectively.

关键词： Ganoderma theaecolum Lanostane nortriterpenoids Neuroprotective effects PTP1B α-Glucosidase

N^(6)-methyladenosine on the natural antisense transcript of niA1 stabilizes its mRNA to boost NO biosynthesis and modulate stomatal movement

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Molecular Plant 2025年第1期18卷 151-165页

作者： Jie Li Wen Tian Ting Chen Qing-yan Liu Hua-Wei Wu Chuan-Hui Liu Yuan-Yuan Fang Hui-Shan Guo Jian-Hua zhao State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijingChina CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijingChina

nitric oxide(NO)is a crucial signaling molecule that regulates a wide range of metabolic pathways in different strata of *** plants,nitrate reductase(NR)is a key enzyme for NO *** are two NR-encoding genes in Arabidopsis genome,niA1 and niA2,which are precisely regulated and expressed in a tissue-specific *** this study,we found that the natural antisense transcript as-niA1,transcribed from the 30 UTR of niA1,stabilizes niA1 mRNA to maintain its circadian oscillation in plants grown under the light/dark ***,as-niA1-dependent niA1 mRNA stability is indispensable for niA1-mediated NO biosynthesis in guard cells and natural stomatal ***,we revealed that polypyrimidine tract-binding 3(PTB3)regulates the stabilization of niA1 mRNA by directly binding to UC-rich elements of *** further found that MTA deposits N^(6)-methyladenosine(m6 A)on as-niA1,facilitating the as-niA1-PTB3 interaction in vivo,in agreement with RNA structure prediction in that m6 Amediated structural alterations expose the UC-rich elements to enhance the accessibility of *** together,these findings reveal a novel molecular mechanism by which plants precisely manipulate NO biosynthesis to modulate light/dark-regulated stomatal movement,highlighting the coupling of RNA epigenetic modifications and structures shaping RNA-protein interactions in the regulation of hormone biosynthesis.

关键词： natural antisense transcript,NO biosynthesis,N^(6)-methyladenosine,niA1,stomatal movement

Liquid refractive index measurement method based on orthogonal polarization laser self-mixing interference

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Optoelectronics Letters 2025年

作者： zhao yan WANG Haipeng DU Shengzhi TONG Jigang KANG Jun School of Electrical Engineering and Automation， Tianjin University of Technology Tianjin Key Laboratory for Control Theory and Applications in Complicated Systems Department of Electrical Engineering， Tshwane University of Technology

In this paper， a novel method combining orthogonal polarization laser self-mixing interference is proposed. The method utilizes a rotating cuvette to measure the refractive index of liquids at different concentrations. The cuvette is filled with the liquid to be measured and rotated by a certain angle. The change in the number of interference fringes， caused by comparing an empty cuvette with a liquid-filled cuvette， is used to calculate the refractive index of the liquid. A four-fold logic subdivision algorithm is then used to improve measurement resolution. The experimental results show that for pure water and different NaCl and glucose solutions concentrations， the average relative errors are 0.47%， 0.59%， and 2.17%， respectively， with the maximum relative error within ±2.54%. The standard deviation of all solutions is less than 3.4%.

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Disruption of Energy Metabolism and Reactive Oxygen Species Homeostasis in Honglian Type-Cytoplasmic Male Sterility(HL-CMS)Rice Pollen

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Rice science 2025年第1期32卷 81-93,I0056-I0058页

作者： WANG Mingyue zhao Weibo FENG Xiaoya CHEN Yi LI Junhao FU Jinmei yan Yingchun CHU zhaohui HUANG Wenchao State Key Laboratory of Hybrid Rice Key Laboratory for Research and Utilization of Heterosis in Indica Ricethe Ministry of Agriculture and Rural Affairs/Department of GeneticsCollege of Life SciencesWuhan UniversityWuhan 430072China

Honglian type-cytoplasmic male sterility(HL-CMS)is caused by the inter-communication between the nucleus and ***,the mechanisms by which sterility genes regulate metabolic alterations and changes in mitochondrial morphology in the pollen of HL-CMS remain *** this study,we compared the morphological differences between the pollen of the male sterile line YA and the near-isogenic line niL-Rf6 using hematoxylin-eosin staining and 4ʹ,6-diamidino-2-phenylindole(DAPI)***-CMS is characterized by gametophytic sterility,where the aborted pollen grains are empty,and the tapetal layer remains *** electron microscopy was employed to observe mitochondrial morphological changes at the microspore stage,revealing significant mitochondrial alterations,characterized by the formation of'large spherical mitochondria',occurred at the binucleate stage in the YA ***,metabolomics analysis revealed decreased levels of metabolites associated with the carbohydrate and flavonoid ***,the decrease in flavonoids was found to contribute to an elevation in reactive oxygen species(ROS)***,we propose a model in which rice fertility is modulated by the levels of pollen carbohydrates and flavonoid metabolites,with impaired mitochondrial energy production and reduced flavonoid biosynthesis as the main causes of ROS accumulation and pollen abortion in rice.

关键词： cytoplasmic male sterility pollen reactive oxygen species rice mitochondrial metabolite

基于脂质组学的不同原料茯茶“发花”前后脂类变化研究

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陕西科技大学学报 2021年第3期39卷 40-47页

作者：赵燕妮陈丹陈雪峰曾桥许牡丹李华胡歆梁艳赵洁妤刘欢陕西科技大学食品与生物工程学院陕西西安710021 南方科技大学公共分析测试中心广东深圳518055 咸阳泾渭茯茶有限公司陕西咸阳712044 河北环境工程学院环境工程系河北秦皇岛066102

“发花”是以冠突散囊菌为主体的发酵过程,也是形成茯茶特殊风味品质的主要工艺.茯茶香气特征与脂类降解产生的众多香气产物密切相关,然而目前对茶叶脂质的研究还缺乏系统性.本研究采用基于超高效液相色谱-四极杆/静电场轨道肼高分辨质... 详细信息

“发花”是以冠突散囊菌为主体的发酵过程,也是形成茯茶特殊风味品质的主要工艺.茯茶香气特征与脂类降解产生的众多香气产物密切相关,然而目前对茶叶脂质的研究还缺乏系统性.本研究采用基于超高效液相色谱-四极杆/静电场轨道肼高分辨质谱(UHPLC-Q Exactive MS)的脂质组学分析方法,对黑茶(T)和杜仲叶(DZ)“发花”前后的样品进行脂质代谢轮廓分析.共鉴定出414个脂质化合物,覆盖共19亚类,包括:神经酰胺(Cer),磷脂酰肌醇(PI),磷脂酰胆碱(PC),甘油二酯(DG),单半乳糖甘油二酯(MGDG),双半乳糖甘油二酯(DGDG),甘油三酯(TG),(O-酰基)-ω-羟基脂肪酸(OAHFA),单半乳糖基单酰基甘油酯(MGMG),溶血磷脂酰胆碱(LPC),溶血磷脂酰甘油(LPG),磷脂酰乙醇胺(PE),溶血磷脂酰乙醇胺(LPE),溶血磷脂酰肌醇(LPI),磷脂酰丝氨酸(PS),磷脂酸(PA),溶血磷脂酸(LPA),磷脂酰甘油(PG),心磷脂(CL).偏最小二乘判别分析(PLS-DA)发现,黑茶和杜仲叶“发花”前后存在显著差异;单变量统计分析表明对于黑茶,“发花”工艺可以提高Cer、MGMG、PA、OAHFA、LPC、LPG、PI、短链TG的含量;降低DGDG、MGDG、LPE、DG、PG、CL、PS、PE、长链TG的含量;对于杜仲叶,“发花”工艺可以提高短链TG、CL、LPA、LPI、PI、LPC、DG、PC、PG、Cer的含量;降低长链TG、MGDG、DGDG的含量.

关键词：茯茶发花黑茶杜仲叶脂质组学超高效液相色谱-四极杆/静电场轨道肼高分辨质谱

Genome-wide circular RNAs signatures involved in sexual maturation and its heterosis in chicken

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Journal of Integrative Agriculture 2025年第2期24卷 697-711页

作者： Yuanmei Wang Jingwei Yuan yanyan Sun Aixin ni Jinmeng zhao Yunlei Li Panlin Wang Lei Shi Yunhe Zong Pingzhuang Ge Shixiong Bian Hui Ma Jilan Chen State Key Laboratory of Animal Biotech Breeding Institute of Animal SciencesChinese Academy of Agricultural Sciences

Sexual maturation heterosis has been widely exploited in animal crossbreeding. However, the underlying mechanism has been rarely explored in chicken. In the present study, we performed the reciprocal crossing between White Leghorn and Beijing You chicken to evaluate the phenotypes related to sexual maturation,and profiled the ovary circRNAs of purebreds(WW, YY) and crossbreds(WY, YW) to elucidate the molecular mechanism underlying heterosis for sexual maturation. Pubic space and oviduct length exhibited positive heterosis,and age at first egg(AFE) exhibited negative heterosis in the crossbreds. We identified 3,025 known circRNAs and 624 putative circRNAs, which were mainly derived from the exons. Among these circRNAs, 141 and 178circRNAs were specially expressed in WY and YW, respectively. There were 52.38 and 64.63% of total circRNAs in WY and YW exhibited non-additive expression pattern, respectively. GO enrichment and KEGG pathway analysis showed that the host genes of non-additive circRNAs were mainly involved in TGF-beta signaling pathway, oocyte development, ATPase activator activity, oocyte meiosis, progesterone-mediated oocyte maturation and GnRH signaling pathway. Weighted gene co-expression network analysis identified that 4 modules were significantly(Pnism u

关键词： chicken sexual maturation heterosis ovary circRNA

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我国羊肚菌的资源现状及开发应用

中国食用菌 2014年第2期33卷 7-9页

作者：陈杭郑林用赵艳妮甘孜州农科所四川康定626000 四川省农业科学院四川成都610066

羊肚菌作为一种营养保健功效很高的食(药)用菌,具有较大开发潜力。对我国羊肚菌的资源现状及开发应用进行了综述,分析了存在的问题,并提出了今后的研究思路。

关键词：羊肚菌资源分布收集利用

Early Crack Propagation Behavior of Laser Metal Deposited Ti-6Al-4V Alloy Under High Cycles Fatigue Loading

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稀有金属材料与工程 2025年第1期54卷 62-75页

作者： Li yanping Huang Wei He yan Xu Feng zhao Sihan Guo Weiguo School of Aeronautics Northwestern Polytechnical UniversityXi'an 710072China School of Astronautics Northwestern Polytechnical UniversityXi'an 710072China

The crack initiation and early propagation are of great significance to the overall fatigue life of *** order to investigate the anisotropic fracture behavior of laser metal deposited Ti-6Al-4V alloy(LMD Ti64)during the early stage,the fourpoint bending fatigue test was carried out on specimens of three different directions,as well as the forged *** results indicate the anisotropic crack initiation and early propagation of LMD *** direction perpendicular to the deposition direction exhibits a better fatigue resistance than the other *** crack initiation position and propagation path are dominated by the microstructure in the vicinity of *** Ti64 has a typical small crack effect,and the early crack propagation velocities in three directions are *** by the slip system of LMD Ti64,secondary cracks frequently occur,which are often found to have an angle of 60°to the main *** electron backscatter diffraction analysis indicates that LMD Ti64 has preferred orientations,i.e.,strong 0001//Z texture and 001//Z *** crystallographic orientation will change as the direction of columnarβgrains turns over,resulting in the fatigue anisotropy of LMD Ti64 in crack initiation and early crack propagation process.

关键词： fatigue resistance crack propagation behavior microstructure laser metal deposit Ti-6Al-4V anisotropy

Based on non-targeted metabolomics for differential components screening of Rosae Chinensis Flos and Rosae Rugosae Flos and their quality evaluation

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Traditional Medicine Research 2025年第2期10卷 1-15页

作者： Xu Liang ni-Hui Zhang Zhi-Lai Zhan Guang-Lu Chang yan Gao Xia Li Wen-Yuan Gao School of Pharmaceutical Science and Technology Faculty of MedicineTianjin UniversityTianjin 300193China State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs China Academy of Chinese Medical SciencesBeijing 100700China Key Laboratory of Modern Chinese Medicine Resources Research Enterprises Tianjin 300402China

Background:Rosa chinensis *** Rosa rugosa *** not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional *** two plants have some differences in efficacy,but the flower buds are easily confused for similar *** addition,large-scale cultivation of ornamental rose flowers may lead to a decrease in the effective components of medicinal ***,it is necessary to study the chemical composition and make quality evaluation of Rosae Chinensis Flos(Yueji)and Rosae Rugosae Flos(Meigui).Methods:In this study,40 batches of samples including Meigui and Yueji from different regions in China were collected to establish high-performance liquid chromatography ***,the fingerprints data was analyzed using principal component analysis,hierarchical cluster analysis,and partial least squares discriminant analysis analysis chemometrics to obtain information on intergroup differences,and non-targeted metabolomic techniques were applied to identify and compare chemical compositions of samples which were chosen from groups with large *** compounds were screened by orthogonal partial least-squares discriminant analysis and S-plot,and finally multi-component quantification was performed to comprehensively evaluate the quality of Yueji and ***:The similarity between the fingerprints of 40 batches roses and the reference print R was 0.73 to 0.93,indicating that there were similarities and differences between the *** principal component analysis and hierarchical cluster analysis of fingerprints data,the samples from different origins and varieties were intuitively divided into four *** least-squares discriminant analysis analysis showed that Meigui and Yueji cluster into two categories and the model was reliable.A total of 89 compounds were identified by high resolution mass spectrometry,mainly were flavonoids and flavon

关键词： Rosa chinensis Jacq. Rosa rugosa Thunb. metabolomics chemometrics multiple component quantification quality evaluation