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One-stitch anastomosis through the skin with bicanalicular intubation:a modified approach for repair of bicanalicular laceration

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International Journal of Ophthalmology(English edition) 2013年第5期6卷 656-658页

作者： Hai Tao Peng Wang Cui Han Jian Zhang Fang Bai zhao-yan he Lacrimal Center of Ophthalmology Armed Police General Hospital of China

AIM:To evaluate the efficacy and safety of one-stitch anastomosis through the skin with bicanalicular silicone tube intubation in repairing of bicanalicular ***:The clinical data of 15 consecutive patients with both superior and inferior canalicular laceration in one eye who underwent surgical repair using one-stitch anastomosis through the skin and bicanalicular stent were retrospective *** the operations were performed under surgical microscope,5-0 silk sutures were used and were with bicanalicular silicone tube(diameter was 8mm) intubation,for one lacerated canaliculi one-stitch anastomosis through the *** stents were left in place for 3 months postoperatively and then *** follow-up period was 3-36 months(average 14 months). RESULTS:In 15 patients,13 patients were cured entirely,1 patient was meliorated,1 patient with no *** patients had got good recovery of eyelid laceration with no traumatic deformity in eyelid and *** was seen in one case,for not followed the doctor’s guidance to come back to hospital to had the suture removed on the 7thday after operation,when he came at the 15thday,the inferior canalicular wall and eyelid skin were corroded by the suture caused 2mm wound,and the inside silicone tube was exposed,a promptly repair with 10-0 nylon suture was done,the wound healed in a *** were no early tube protrusions and punctal slits in the ***:One-stitch anastomosis through the skin with bicanalicular silicone tube intubation is a good method in repair of bicanalicular laceration in one eye,the cut ends can be anastomosed directly,and with excellent cosmetic results,it is acceptable for the *** there is no suture remained in the wound permanently,so there is no suture-related granuloma which may cause obstruction or stenosis of *** is simple,economical,effective and safe.

关键词： both superior and inferior canalicular laceration repair one-stitch anastomosis through the skin bicanalicular intubation

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Identification and Promoter Activity Analysis of Porcine miR-181 and miR-1

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Journal of Integrative Agriculture 2012年第6期11卷 986-992页

作者： ZHANG Hai-xin ZHANG Rui LIU Yi-nan WANG Dao-lin zhao yan-he REN Zhu-qing XIONGYuan-zhu Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture/Key Laboratory of Agriculture Animal Genetics Breeding and Reproduction Ministry of Education/College of Animal Science Huazhong Agricultural University Wuhan 430070 P.R. China College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 P.R.China

Since its discovery a decade ago, microRNA has been identified as one of the major regulatory gene families in eukaryotic cells. Many functions of microRNAs have been revealed both in flora and fauna in recent years, but the transcriptional regulation of microRNA genes is not well-understood. In the present study, a series of primers were designed in the 2 000 nt upstream regions of porcine miR-181 and miR-1 and then the sequences were cloned into pGL3-basic vector to test their transcriptional activity. Dual-luciferase reporter assays showed that, the activity of 5＂-flanking sequence of miR-181 started on construct -51, decreasing with the length of the fragment up to -444. The upstream 590 bp confer maximal transcriptional activity and the basal promoter activity is located within the -82 to ＋16 bp region. For miR-1, the activity starts on construct -50, decreasing with the length of the fragment up to -1 254 in despite of a bit of fluctuation, and the basal promoter activity is located within the -50 to ＋47 bp region. Furthermore, some putative regulation elements of both miR-181 and miR-1 were located. In addition, tissue distribution revealed that miR-181 is expressed at a relatively low level.

关键词： miR-181 miR-1 pigs promoter transcriptional activity

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Multifunctional Nacre‑Like Nanocomposite Papers for Electromagnetic Interference Shielding via heterocyclic Aramid/MXene Template‑Assisted In‑Situ Polypyrrole Assembly

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Nano-Micro Letters 2025年第3期17卷 37-54页

作者： Jinhua Xiong Xu zhao Zonglin Liu he Chen Qian yan Huanxin Lian Yunxiang Chen Qingyu Peng Xiaodong he National Key Laboratory of Science and Technology On Advanced Composites in Special Environments Center for Composite Materials and StructuresHarbin Institute of TechnologyHarbin 150080People’s Republic of China

Robust, ultra-flexible, and multifunctional MXene-basedelectromagnetic interference (EMI) shielding nanocomposite filmsexhibit enormous potential for applications in artificial intelligence,wireless telecommunication, and portable/wearable electronic *** this work, a nacre-inspired multifunctional heterocyclic aramid(HA)/MXene@polypyrrole (PPy) (HMP) nanocomposite paper withlarge-scale, high strength, super toughness, and excellent tolerance tocomplex conditions is fabricated through the strategy of HA/MXenehydrogel template-assisted in-situ assembly of PPy. Benefiting from the"brick-and-mortar" layered structure and the strong hydrogen-bondinginteractions among MXene, HA, and PPy, the paper exhibits remarkable mechanical performances, including high tensile strength (309.7 MPa),outstanding toughness (57.6 MJ m−3), exceptional foldability, and structural stability against ultrasonication. By using the template effect ofHA/MXene to guide the assembly of conductive polymers, the synthesized paper obtains excellent electronic conductivity. More importantly,the highly continuous conductive path enables the nanocomposite paper to achieve a splendid EMI shielding effectiveness (EMI SE) of 54.1 dBat an ultra-thin thickness (25.4 μm) and a high specific EMI SE of 17,204.7 dB cm2g−1. In addition, the papers also have excellent applicationsin electromagnetic protection, electro-/photothermal de-icing, thermal therapy, and fire safety. These findings broaden the ideas for developinghigh-performance and multifunctional MXene-based films with enormous application potential in EMI shielding and thermal management.

关键词： MXene Remarkable mechanical properties heterocyclic aramid Electromagnetic interference shielding Polypyrrole Multifunctionality

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Identification of Residue Involved in Nucleotidyltransferase Activity of LinA from Staphylococci by Site-directed Mutagenesis

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Chinese Journal of Structural Chemistry 2019年第3期38卷 422-428页

作者： ZOU Xiao-Ming JIANG Mei-Qin zhao yan-he SUN Li-Fang yanG Wen-Di YE Xian-Ren WU Yun-Kun State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences Department of Cell Biology and Biophysics University of Texas Southwestern Medical Center Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation College of Life Science Fujian Normal University Fujian Cancer Hospital & Fujian Medical University Cancer Hospital

The lincosamide class of antibacterials is widely used for the treatment of a broad spectrum of infections, and one prevalent route of resistance to lincosamides in pathogenic gram-positive cocco is antibiotic modification. Enzymes encoded by lin genes, belonging to nucleotidyltransferase superfamily, catalyze adenylylation to inactivate lincosamides. LinA can adenylylate lincosamides at either 3?-or 4?-OH of the methylthiolincosamide sugar. The crystal structure of LinA/lincomycin has confirmed its active site. However, the residue interacting with nucleotidyl donors remains elusive. here, we modeled the complex structure of LinA/lincomycin/Mg^(2+)/AMPCPP to reveal a putative pocket for nucleotidyl donors and suggested the residue R45 in this pocket involved in the recognition of donor substrates NTP and catalysis. ITC and enzyme activity assays show that the mutation of residue R45 impairs LinA nucleotidyltransferase activity in vitro. This work provides insights into the molecular mechanism of the nucleotide binding and transferring activity of antibiotic NTases.

关键词： LinA nucleotidyltransferase site-directed mutagenesis enzymatic assay ITC

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备用信用证担保人民币贷款的性质、条件及风险控制

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税务与经济 2006年第1期 83-86页

作者：赵延河深圳发展银行北京分行国际部北京100031

备用信用证贷款是由开证行承担独立、第一性付款责任的外汇、信用保证贷款,其担保的有效性决定了融资业务的低风险特征。认识备用证及其担保作用,明确业务性质,是确立信贷条件和有效控制风险的必要前提,也是规范业务发展的基本要求。

关键词：备用信用证贷款风险控制担保

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Establishing a new testing protocol of the electrochemical stability window for electric double-layer capacitors

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Science China Chemistry 2025年第1期68卷 217-225页

作者： Ruilin Hou Xiaoxi zhao Tingting Liang Qingyun Dou Shan Xu Xingbin yan School of Materials Science and Engineering Sun Yat-sen University Laboratory of Clean Energy Chemistry and Materials Lanzhou Institute of Chemical Physics Chinese Academy of Sciences College of Engineering and Applied Sciences Nanjing University Center of Industrial Analysis and Testing Guangdong Academy of Science

The electrochemical stability window(ESW) is crucial for determining the energy density of electric double-layer capacitors(EDLCs). However, current ESW testing protocols of EDLCs are strongly affected by artificial factors, thereby leading to unreliable result. To solve this problem, we first identify the key shortcomings of the traditional protocols by analyzing their test principles. Subsequently, taking an aqueous carbon-based EDLC as an example, we design a side reaction descriptor to represent the response difference in the charging capacitance to side reactions between the cyclic voltammetry and galvanostatic chargedischarge methods. With the aid of this descriptor, we propose a new ESW testing protocol, which is not only able to completely avoid the adverse influence of subjective factors but also fully consider the operating condition limitations. The protocol can be extended from the single electrode to the full device. This work proposes a more rigorous definition of ESW and provides a reliable protocol for evaluating the ESW of EDLCs.

关键词： electric double-layer capacitors electrochemical stability window testing protocol side reaction descriptor operating limitation

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对日美贸易摩擦的回顾与展望

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现代日本经济 2001年第4期118卷 1-5,44页

作者：赵春明何艳北京师范大学经济学院北京 100875

日美贸易摩擦是战后日美经济贸易关系中一个非常突出的问题，是日美全部经济摩擦的基点，在相当程度上决定着两国其它方面摩擦的产生、发展和解决方式。本文将在简要回顾日美贸易摩擦的历史发展进程的基础上，分析其特点及其产生根源，... 详细信息

日美贸易摩擦是战后日美经济贸易关系中一个非常突出的问题，是日美全部经济摩擦的基点，在相当程度上决定着两国其它方面摩擦的产生、发展和解决方式。本文将在简要回顾日美贸易摩擦的历史发展进程的基础上，分析其特点及其产生根源，并进一步探讨新世纪日美贸易摩擦的发展趋势。

关键词：日美贸易摩擦发展趋势日本美国

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Channel pruning on frequency response

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Science China(Information Sciences) 2025年第1期68卷 159-170页

作者： Hang LIN Yifan PENG Lin BIE Chenggang yan Xibin zhao Yue GAO School of Software Tsinghua University School of Automation Hangzhou Dianzi University

Network pruning has a significant role in reducing network parameters and accelerating the inference time of the network. Some existing methods prune the network based on the frequency of the data, and finally obtain a sub-network with high accuracy. However, according to our experimental analysis, different frequencies of information in the data contribute differently to the accuracy of the model, and using this information directly for pruning without making a selection will lead to incorrect results. We believe that pruning should retain the convolutional kernels in the network that process important information, while those kernels that process unimportant information should be removed. In this paper, we first investigate the meaning of each frequency band information in the spectrum and their contribution to the prediction accuracy of the network,and according to these results, we propose a new pruning method based on frequency response(PFR). Our PFR finds and removes the convolutional kernels in the network that specialize in processing unimportant information, resulting in a compact neural network model. PFR obtains significant experimental results on different datasets, for example, a 56.0% raduction of float points operations(FLOPs) on Res Net-50 and only 0.37% of Top-1 accuracy degradation on the Image Net dataset.

关键词： deep learning model compression filter pruning channel pruning frequency response

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“Zero‑Strain” NiNb_(2)O_(6) Fibers for All‑Climate Lithium Storage

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Nano-Micro Letters 2025年第1期17卷 348-360页

作者： yan zhao Qiang Yuan Liting yang Guisheng Liang Yifeng Cheng Limin Wu Chunfu Lin Renchao Che College of Physics Donghua UniversityShanghai 201620People’s Republic of China School of Materials Science and Engineering Institute of Materials for Energy and EnvironmentQingdao UniversityQingdao 266071People’s Republic of China Laboratory of Advanced Materials Shanghai Key Laboratory of Molecular Catalysis and Innovative MaterialsAcademy for Engineering&TechnologyFudan UniversityShanghai 200438People’s Republic of China Zhejiang Laboratory Hangzhou 311100People’s Republic of China Inner Mongolia University Hohhot 010021People’s Republic of China

Niobates are promising all-climate Li^(+)-storage anode material due to their fast charge transport,large specific capacities,and resistance to electrolyte ***,their moderate unit-cellvolume expansion(generally 5%–10%)during Li^(+)storage causes unsatisfactory long-term ***,“zero-strain”NiNb_(2)O_(6) fibers are explored as a new anode material with comprehensively good electrochemical *** Li^(+)storage,the expansion of electrochemical inactive NiO_(6) octahedra almost fully offsets the shrinkage of active NbO_(6) octahedra through reversible O *** superior volume-accommodation capability of the NiO_(6) layers guarantees the“zero-strain”behavior of NiNb_(2)O_(6) in a broad temperature range(0.53%//0.51%//0.74%at 25//−10//60℃),leading to the excellent cyclability of the NiNb_(2)O_(6) fibers(92.8%//99.2%//91.1%capacity retention after 1000//2000//1000 cycles at 10C and 25//−10//60℃).This NiNb_(2)O_(6) material further exhibits a large reversible capacity(300//184//318 mAh g−1 at 0.1C and 25//−10//60℃)and outstanding rate performance(10 to 0.5C capacity percentage of 64.3%//50.0%//65.4%at 25//−10//60℃).Therefore,the NiNb_(2)O_(6) fibers are especially suitable for large-capacity,fast-charging,long-life,and all-climate lithium-ion batteries.

关键词： NiNb_(2)O_(6)porous fiber “Zero-strain”mechanism Electrochemical property Harsh-temperature operation Operando characterization

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基于拉曼光谱技术的枇杷果实β-胡萝卜素含量无损测定研究

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光谱学与光谱分析 2016年第11期36卷 3572-3577页

作者：王涛裘正军张卫正赵艳茹何勇浙江大学生物系统工程与食品科学学院浙江杭州310058

β-胡萝卜素作为一种重要的营养元素,其在果蔬活体内的测定方法通常必须对样品进行破坏,并且耗时、费力。采用拉曼光谱技术预测活体枇杷果实内β-胡萝卜素含量,以高效液相色谱法(HPLC)检测值作为参考值,采用高斯平滑对光谱数据进行预处... 详细信息

β-胡萝卜素作为一种重要的营养元素,其在果蔬活体内的测定方法通常必须对样品进行破坏,并且耗时、费力。采用拉曼光谱技术预测活体枇杷果实内β-胡萝卜素含量,以高效液相色谱法(HPLC)检测值作为参考值,采用高斯平滑对光谱数据进行预处理,同时使用多项式拟合(PF)的方法对光谱数据进行背景荧光扣除,以标准β-胡萝卜素的拉曼图谱为基础,选取了三个特征频移,以特征频移和截取的光谱建立了MLR模型、PLSR模型以及LS-SVM模型,取得了比较好的预测精度。其中LS-SVM模型的预测精度最高,其预测相关系数R_p^2达到了0.91,表明通过拉曼光谱对活体枇杷内β-胡萝卜素的含量检测是实际可行的。

关键词： β-胡萝卜素枇杷拉曼光谱 HPLC

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