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Food Bioscience 2022年第5期49卷 616-625页

作者： Hui Zhan Wenfeng Xu xiangxiang zhao Linlin Tian Fen Zhang Hua Wei Xueying Tao State Key Laboratory of Food Science and Technology Nanchang UniversityNanchang330047China

Artemisia argyi is a traditional Chinese food and medicine which can improve multiple diseases such as asthma and gastrointestinal ***,whether the beneficial bioactivities are associated with microbial fermentation remain *** objective of this study was to investigate the effects of fermentation on bioactive compounds,antioxidant activity,and flavor characteristics of Artemisia argyi fermentation liquid(AAFL-LP)by an autochthonous strain,Lactiplantibacillus plantarum *** WLPL01 was found to utilize sugars and flavonoids to produce abundant organic acids,such as lactic acid and malic acid,improve antioxidant activity of Artemisia argyi including total superoxide dismutase content,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical-scavenging capacity,and Fe-reducing antioxidant power,decrease largely bitter taste notes such as leucine,isoleucine,and phenylalanine,and enhance fruity,sweet,and flora notes such as terpenes and esters of ***,*** WLPL01 could improve the functionality of *** and the flavor of the product,suggesting it a promising candidate in developing functional *** food.

关键词： Artemisia argyi Lactiplantibacillus plantarum WLPL01 Fermentation Antioxidant capacity Bioactive compounds

RNA-seq profiling the transcriptome of secondary seed dormancy in canola(Brassica napus L.)

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Chinese Science Bulletin 2014年第32期59卷 4341-4351页

作者： Fuxia Liu Xiangqiang zhao Lihua Zhang Tang Tang Changming Lu Guiming Chen Xinlong Wang Cuiping Bu xiangxiang zhao Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake Huaiyin Normal University School of Life Sciences Nantong University Plant science department South Dakota State UniversityBrookings SD 57006 USA Genetic Engineering and GMO Biosafety Lab of Oil Crops Research Institute Chinese Academy of Agricultural Sciences

Secondary seed dormancy(SSD)is responsible for volunteer plants in canola fields,which causes a series of problems in canola production and serves as an important trait for the environmental safety assessment of transgenic canola.A canola cultivar with strong SSD was used to establish insight into seed transcriptomes in its secondarily dormant seeds and control seeds without dormancy by RNA-seq analysis,aiming to determine the molecular ecological characterizations of SSD.A dataset(more than 4 Gb)of valid sequences was obtained from each sample,which was combined to carry out the de novo *** assembled sequences consisted of 314,261fragments with length[100 bp,including 29,740 long transcripts of length C 500 *** annotation indicated that 1,641 long transcripts could be categorized into 24 cluster of orthologous groups of proteins(COGs)and 16,515 transcripts were linked to 2,648 gene ontology(GO)*** were 452 long transcripts with significantly different expression identified by a threshold of[2-fold expression change(P\0.001)between samples,among which 343 transcripts were unambiguously homologous to Arabidopsis *** plant hormones abscisic acid and gibberellins were known as the pivotal regulators of seed dormancy and *** genes responsible for either biosynthesis or signaling of each hormone could be widely verified from the SSD transcriptome,theirs expression evidences failed to correlate with the induction of *** on the enriched terms of gene ontology and KEGG orthology,as well as the expression models of candidate genes of SSD,we proposed that fatty acid metabolism might implicate in SSD in *** information reported here may play a significant role in further understanding of environmental safety assessment of SSD in transgenic canola.

关键词：种子休眠转录物油菜田二级 Q分析转基因油菜双低油菜籽基因本体论

Rationalizing the isolation distance needed for field trials involving genetically modified rapeseed (Brassica napus L.) in China

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Chinese Science Bulletin 2013年第13期58卷 1558-1567页

作者： zhao xiangxiang TANG Tang CHEN GuiMin LIU FuXia WANG XingLong BU CuiPing LU ChangMing Key Laboratory of Oil Crop Biology of Ministry of Agriculture Oilcrops Research InstituteChinese Academy of Agricultural Sciences Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake Huaiyin Normal University

The isolation distance required for field trials of genetically modified (GM) rapeseed varies widely worldwide, with a 50-400 m distance in most nations contrasting with a minimum 1000-m isolation distance in China. The goal of this study was to evaluate the relevance of current regulations in China regarding the isolation distance needed for GM rapeseed trials. A pollen flow experiment was conducted based on the design of concentric circles, with the GM plants in a 20-m diameter circle at the centre, surrounded by non-GM plants to a distance 60 m from the perimeter of the circle containing GM plants. The rate of pollen flow was the highest at the isolation distance of 0.5 m, where it ranged from 2.3091% to 2.6711%. The general pattern of the pollen flow rate (y) with distance (x) was well described by the equation y = 1.3936x-0.9136 (R 2 = 0.9950). The long-distance pollen flow tested at the isolation distance of 800 m was 0.0018%, which agrees with the theoretical prediction. The results suggested that 300 m, rather than 1000 m, is a reasonable distance to ensure a tolerable threshold of pollen flow (less than 0.01%) under conditions of winter rapeseed production in China.

关键词：转基因油菜籽隔离距离现场试验中国转基因植物花粉流世界范围田间试验

Assessment on alien herbicide-resistant gene flow among crucifers by sexual compatibility

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Chinese Science Bulletin 2005年第15期50卷 1604-1611页

作者： zhao xiangxiang LU Weiping QI Cunkou PU Huiming XIA Qiuxia LU Dalei LIU Geshan WANG Youping Jiangsu Provincial Key Lab of Crop Genetics and Physiology Yangzhou University Yangzhou 225009 China Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences Nanjing 210014 China College of Bioscience and Biotechnology Yangzhou UniversityYangzhou 225009 China

Cross-pollination and high cross-compatibility are frequently found in many cruciferous plants, which im-plies that there might be potential risk of alien gene flow among crucifers. In this work, the alien glyphosate-resistant gene flow between GM (Genetically modified) rapeseed vari-ety Q3 (Brassica napus) and 6 varieties from 5 cruciferous species was assessed by sexual compatibility through aniline blue fluorescence observation, manual cross, mentor pollina-tion with the mixture pollen and DNA identification. The results indicated that the compatibility indices of two B. napus varieties, B. juncea var. multiceps Tsen et Lee and Chinese cabbage (B. chinensis) (as female) with Q3 (as male) were 16.15, 12.77, 2.345 and 0.85, respectively. F1 plants were examined by PCR and all of these samples were confirmed as positive. Crossing between Orychophragmus violaceus, Des-curainia sophia (as female) and Q3 (as male) expressed high cross-incompatibility. Fluorescence microscope observation indicated that growing of pollen tube terminated at the upper 1/3 part of the style of O. violaceus and on the stigma surface of D. sophia. The mentor plants were further analyzed by PCR and all were shown to be negative. Under the neighboring growth and natural pollination conditions, the rates of gene flow to two B. napus varieties were 2.3278% and 2.1487%, respectively, B. juncea var. multiceps Tsen et Lee and Chinese cabbage were 1.0157% and 0.9236%, re-spectively. No gene flow was observed in O. violaceus and D. sophia. Frequency of gene flow was highly correlated with sexual compatibility. Due to possible alien gene flow among crucifers, special consideration and care should be taken to grow GM rapeseed.

关键词：除草剂十字花科植物荧光性遗传因子

Investigation of CRISPR/Cas9-induced SD1 rice mutants highlights the importance of molecular characterization in plant molecular breeding

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Journal of Genetics and Genomics 2020年第5期47卷 273-280页

作者： Sukumar Biswas Jiaqi Tian Rong Li Xiaofei Chen Zhijing Luo Mingjiao Chen xiangxiang zhao Dabing Zhang Staffan Persson Zheng Yuan Jianxin Shi Joint International Research Laboratory of Metabolic&Developmental Sciences Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and HealthState Key Laboratory of Hybrid RiceSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai200240China Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental ProtectionHuaian223300China School of Biosciences University of MelbourneParkville VIC3010MelbourneAustralia

Although Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/CRISPR-associated 9(Cas9)system has been widely used for basic research in model plants,its application for applied breeding in crops has faced strong regulatory obstacles,due mainly to a poor understanding of the authentic output of this system,particularly in higher *** this study,different from any previous studies,we investigated in detail the molecular characteristics and production performance of CRISPR/Cas9-generated SD1(semi-dwarf 1)mutants from T2 to T4 generations,of which the selection of T1 and T2 was done only by visual phenotyping for semidwarf *** data revealed not only on-and off-target mutations with small or lager indels but also exogenous elements in T2 *** indel mutants passed stably to T3 or T4 without additional modifications independent on the presence of Cas9,while some lines displayed unexpected hereditary patterns of Cas9 or some exogenous *** addition,effects of various SD1 alleles on rice height and yield differed depending on genetic *** together,our data showed that the CRISPR/Cas9 system is effective in producing homozygous mutants for functional analysis,but it may be not as precise as expected in rice,and that early and accurate molecular characterization and screening must be carried out for generations before transitioning of the CRISPR/Cas9 system from laboratory to field.

关键词： CRISPR/Cas9 Plant height Oryza sativa Semi-dwarf 1 Yield

OsMADS32 interacts with PI-like proteins and regulates rice flower development

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Journal of Integrative Plant Biology 2015年第5期57卷 504-513页

作者： Huanhuan Wang Liang Zhang Qiang Cai Yun Hu Zhenming Jin xiangxiang zhao Wei Fan Qianming Huang Zhijing Luo Mingjiao Chen Dabing Zhang Zheng Yuan State Key Laboratory of Hybrid Rice School of Life Sciences and BiotechnologyShanghai Jiao Tong University College of Life and Basic Sciences Sichuan Agricultural University Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake Huaiyin Normal University

OsMADS32 is a monocot specific MIKCc type MADS‐box gene that plays an important role in regulating rice floral meristem and organs identity, a crucial process for reproductive success and rice yield. However, its underlying mechanism of action remains to be clarified. Here, we characterized a hypomorphic mutant allele of OsMADS32/CFO1, cfo1‐3 and identified its function in controlling rice flower development by bioinformatics and protein‐protein interaction analysis. The cfo1‐3 mutant produces defective flowers, including loss of lodicule identity, formation of ectopic lodicule or hull‐like organs and decreased stamen number, mimicking phenotypes related to the mutation of B class genes. Molecular characterization indicated that mis‐splicing of OsMADS32 transcripts in the cfo1‐3 mutant resulted in an extra eight amino acids in the K‐domain of OsMADS32 protein. By yeast two hybrid and bimolecular fluorescence comple-mentation assays, we revealed that the insertion of eight amino acids or deletion of the internal region in the K1 subdomain of OsMADS32 affects the interaction between OsMADS32 with PISTILLATA （PI）‐like proteins OsMADS2 and OsMADS4. This work provides new insight into the mecha-nism by which OsMADS32 regulates rice lodicule and stamen identity, by interaction with two PI‐like proteins via its K domain.

关键词： Floral organ identity hypomorphic mutant K domain OsMADS32 protein interaction

Interactions of OsMADS1 with Floral Homeotic Genes in Rice Flower Development

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Molecular Plant 2015年第9期8卷 1366-1384页

作者： Yun Hu Wanqi Liang Changsong Yin Xuelian Yang Baozhe Ping Anxue Li Ru Jia Mingjiao Chen Zhijing Luo Qiang Cai xiangxiang zhao Dabing Zhang Zheng Yuan State Key Laboratory of Hybrid Rice Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health School of Life Sciences andBiotechnology Shanghai Jiao Tong University Shanghai 20040 China Shanghai Ocean University Shanghai 201306 China jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection Huaiyin Normal University Huaian 223300 China School of Agriculture Food and Wine University of Adelaide Waite Campus Urrbrae SA 5064 Australia

During reproductive development, rice plants develop unique flower organs which determine the final grain yield. OsMADS1, one of SEPALLATA-like MADS-box genes, has been unraveled to play critical roles in rice floral organ identity specification and floral meristem determinacy. However, the molecular mechanisms underlying interactions of OsMADS1 with other floral homeotic genes in regulating flower development remains largely elusive. In this work, we studied the genetic interactions of OsMADS1 with B-, C-, and D-class genes along with physical interactions among their proteins. We show that the physical and genetic interactions between OsMADS1 and OsMADS3 are essential for floral meristem activity maintenance and organ identity specification; while OsMADS1 physically and genetically interacts with OsMADS58 in regu- lating floral meristem determinacy and suppressing spikelet meristem reversion. We provided important genetic evidence to support the neofunctionalization of two rice C-class genes （OsMADS3 and OsMADS58） during flower development. Gene expression profiling and quantitative RT-PCR analyses further revealed that OsMADS1 affects the expression of many genes involved in floral identity and hormone signaling, and chromatin immunoprecipitation （ChlP）-PCR assay further demonstrated that OsMADS17 is a direct target gene of OsMADS1. Taken together, these results reveal that OsMADS1 has diversified regulatory functions in specifying rice floral organ and meristem identity, probably through its genetic and physical interactions with different floral homeotic regulators.

关键词： OsMADS1 floral homeotic genes floral organ identity floral meristem regulatory network

Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection

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Synthetic and Systems Biotechnology 2021年第4期6卷 283-291页

作者： xiangxiang zhao Zhengduo Wang Bowen Yang Zilong Li Yaojun Tong Yuhai Bi Zhenghong Li Xuekui Xia Xiangyin Chen Lixin Zhang Weishan Wang Gao-Yi Tan State Key Laboratory of Bioreactor Engineering and School of BiotechnologyEast China University of Science and Technology(ECUST)Shanghai200237China State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing100101China State Key Laboratory of Microbial Metabolism and School of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai200240China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyCenter for Influenza Research and Early-warning(CASCIRE)CAS-TWAS Center of Excellence for Emerging Infectious Diseases(CEEID)Chinese Academy of SciencesBeijing100101China Key Biosensor Laboratory of Shandong Province Biology InstituteQilu University of Technology(Shandong Academy of Sciences)Jinan250013China

Antigen detection provides particularly valuable information for medical diagnoses;however,the current detection methods are less sensitive and accurate than nucleic acid *** combination of CRISPR/Cas12a and aptamers provides a new detection paradigm,but sensitive sensing and stable amplification in antigen detection remain ***,we present a PCR-free multiple trigger dsDNA tandem-based signal amplification strategy and a de novo designed dual aptamer synergistic sensing *** of these two strategies endowed the CRISPR/Cas12a and aptamer-based method with ultra-sensitive,fast,and stable antigen *** a demonstration of this method,the limit of detection was at the single virus level(0.17 fM,approximately two copies/μL)in SARS-CoV-2 antigen nucleocapsid protein analysis of saliva or serum *** entire procedure required only 20 *** our system’s simplicity and modular setup,we believe that it could be adapted reasonably easily for general applications in CRISPR/Cas12a-aptamer-based detection.

关键词： CRISPR/Cas12a Aptamer Antigen detection SARS-CoV-2 PCR-Free amplification Synergistic sensing

Value of identifying and diagnosing mammary carcinoma and non-lactation mastitis lump by color Doppler ultrasonography

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The Chinese-German Journal of Clinical Oncology 2008年第11期7卷 638-640页

作者： Guilong Jin Zhiwei Ding Yuxia Guo xiangxiang zhao Department of Ultrasonography Dongyang People＇s Hospital Dongyang 322100 China

Objective: To evaluate the value of identifying and diagnosing mammary carcinoma and non-lactation mastitis lump (NLM) by multicolor Doppler ultrasonography. Methods: We compared and analyzed the examination results of 69 cases of mammary carcinoma proved by surgical pathology and 22 cases of NLM before surgery by multicolor Doppler ultra-sonography. Results: The detection rates of mammary carcinoma and NLM focus by ultrasonic examining were 100%. The shape, envelope, foul line, blood stream between sound and image of two diseases were similar. Of the two diseases, the representation with slight calcification in lump, lower echo in low echo, bloodstream distribution and resistance were different. Conclusion: By analyzing the sound and image representation of mammary lump, we find color Doppler ultrasonography has significant value in identifying and diagnosing mammary carcinoma and non-lactation mastitis lump.

关键词： mammary carcinoma non-lactation mastitis lump (NLM) color Doppler ultrasonography

双重食欲素受体拮抗剂lemborexant治疗失眠障碍临床研究进展

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中华神经科杂志 2022年第6期55卷 659-664页

作者：赵翔翔赵忠新海军军医大学附属长海医院神经内科上海200433 海军军医大学附属长征医院神经内科上海200003

在过去的20年中,人们对食欲素系统在调节睡眠和觉醒方面作用的了解迅速增加。lemborexant作为双重食欲素受体拮抗剂已在部分国家被批准用于治疗失眠障碍,现有研究显示其安全性和耐受性与传统催眠药物相比具有显著优越性,将为临床失眠障... 详细信息

在过去的20年中,人们对食欲素系统在调节睡眠和觉醒方面作用的了解迅速增加。lemborexant作为双重食欲素受体拮抗剂已在部分国家被批准用于治疗失眠障碍,现有研究显示其安全性和耐受性与传统催眠药物相比具有显著优越性,将为临床失眠障碍的治疗提供新的手段。文中对双重食欲素受体拮抗剂lemborexant的药理学、临床疗效和安全性等方面进行综述。

关键词：睡眠障碍治疗临床研究性药理学食欲素受体拮抗剂