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Verification and fine-mapping of QTLs conferring days to flowering in soybean using residual heterozygous lines

Chinese Science Bulletin 2010年第6期55卷 499-508页

作者： SU ChengFu LU WeiGuo zhao tuanjie GAI JunYi Soybean Research Institute Nanjing Agricultural University National Center for Soybean Improvement National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing 210095 China Institute of Cotton and Oil Crops Henan Academy of Agricultural Sciences Zhengzhou 450002 China

The results of QTL mapping based on a primary mapping population should be further verified and refined for its real utilization in marker-assisted selection or map-based *** primary mapping population contains 114 BC1F1 plants of the backcross between Essex (maturity group V,MG V) as the donor parent and ZDD2315 (MG II) as the recurrent *** this study,a genetic linkage map with 250 SSR markers spanning a total length of 2963.5 cM on 25 linkage groups (LG) was constructed using software *** kinds of genetic statistical models of 4 softwares,*** Cartographer Version 2.5,IciMapping Version 2.0,MapQTL Version 5.0 and QTLnetwork Version 2.0,were used to map QTLs conferring days to flowering of the BC1F3 *** QTLs were mapped on 6 different linkage groups (LG).Of those,6 QTLs were detected by at least two different genetic statistical models,while the other three were detected by only one *** the three QTLs,Flwdt7 was mapped between Sat_213 and Satt643 on LG C2 with only 11.0% contribution *** confirmation of Flwdt7,5 RHL populations were developed through selfing eight BC1F5 plants heterozygous at seven markers around the *** RHL populations with the same segregating loci were bulked and used to construct a secondary linkage map of the specific segment using software JoinMap *** genetic distances among the markers on the specific segment became shorter than those of the whole genome *** the secondary map,Flwdt7 was mapped between Satt277 and Satt489,next to its primary interval Sat_213-Satt643,with distance 1.40 cM to Satt277 and 0.45 cM to Satt489,confidence interval narrowed to 2.7 cM,and contribution rate increased to 36.8%.The results were confirmed with significance analysis among marker genotypes on individual loci and comparison analysis of target marker intervals among near isogenic lines (plants).Thus the strategy by using residual heterozygous lines for QTL fine-mapping on target segments ba

关键词：大豆回交线 RHL (剩余异质接合的线) SSR (简单顺序重复) 标记 flowering 日期 QTL 好印射

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Domestication or Foreignization:A Cultural Choice——On the Nickname Translation in Sidney Shapiro's Outlaws of the Marsh

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校园英语 2020年第1期 220-221页

作者： zhao tuanjie International Education School of Wuchang Institute of Technology

Through detailed statistics and analysis about the translation of 108 heroes’ nicknames, the author manages to probe into the rendering of nicknames in Outlaws of the Marsh from the angle of foreignization and domestication theory. And in Outlaws of the Marsh, Sidney Shapiro managed to achieve a balance between domestication and foreignization in translating the nicknames.

关键词： domestication foreignization Sidney Shapiro Outlaws of the Marsh nicknames

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Mutations in the WUSCHEL-related homeobox1 gene cause an increased leaflet number in soybean

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The Crop Journal 2024年第6期12卷 1645-1654页

作者： Yawei Li Li Chu Peiyun Lyu Wenhuan Lyu Ping Xie Chunting Zhang Haoxian Feng Bin Liu tuanjie zhao Key Laboratory of Eology and Genetis Improverment of Soybear Ministry of Agriculure/Zhonghur Biological Deeditg Labor atory(乙BBL)/Narionol hnnowation Playform for Soybeat Breedirg and Industry-Education Integrution/Slate Key Laboratory of Crop Generics&Germplasm Ethancement and Uuilzation/College of AgriculureNanjirg Agr iculmural UhtiversityNanjing 210095JinguChina State Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

WUSCHEL-related homeobox(WOX)transcription factors play a crucial role in lateral organ development in several plant species;however,their precise functions in soybean(Glycine max[L.]Merr.)were ***,we identified two independent multi-leaflet soybean mutants,mlw48-8 and mlw48-161,from a CRISPR/Cas9-engineered mutant library in the Williams 82 *** mutants exhibited irregular leaf margins,and the upper leaves were narrow and almost lanceolate at *** analysis revealed that these are allelic mutants with independent mutations in the WUSCHEL-related homeobox1(GmWOX1A)gene.A transcriptome analysis demonstrated that GmWOX1A modulates the expression of auxin-and leaf development–related *** two-hybrid and split-luciferase complementation imaging assays revealed that GmWOX1A interacts with the YABBY family protein GmYAB5,providing further evidence of its potential involvement in leaf ***,the mlw48-161 mutant showed an increased seed number per ***,our study not only provides valuable insights into the role of GmWOX1A in soybean leaf development but also offers a potential strategy for high-yield breeding.

关键词： Soybean GmWOX1A GmYAB5 Compound leaf development Seed number per plant

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An optimized protocol for stepwise optimization of real-time RT-PCR analysis

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Horticulture Research 2021年第1期8卷 2474-2494页

作者： Fangzhou zhao Nathan A.Maren Pawel Z.Kosentka Ying-Yu Liao Hongyan Lu James R.Duduit Debao Huang Hamid Ashrafi tuanjie zhao Alejandra I.Huerta Thomas G.Ranney Wusheng Liu Soybean Research Institute Nanjing Agricultural University210095 NanjingChina Department of Horticultural Science North Carolina State UniversityRaleighNC 27607USA Mountain Crop Improvement Lab Department of Horticultural ScienceMountain Horticultural Crops Research and Extension CenterNorth Carolina State UniversityMills RiverNC 28759USA Department of Entomology and Plant Pathology North Carolina State UniversityRaleighNC 27607USA College of Biosystems Engineering and Food Science Zhejiang University310058 HangzhouChina

Computational tool-assisted primer design for real-time reverse transcription(RT)PCR(qPCR)analysis largely ignores the sequence similarities between sequences of homologous genes in a plant *** can lead to false confidence in the quality of the designed primers,which sometimes results in skipping the optimization steps for ***,the optimization of qPCR parameters plays an essential role in the efficiency,specificity,and sensitivity of each gene’s ***,we proposed an optimized approach to sequentially optimizing primer sequences,annealing temperatures,primer concentrations,and cDNA concentration range for each reference(and target)*** approach started with a sequence-specific primer design that should be based on the single-nucleotide polymorphisms(SNPs)present in all the homologous sequences for each of the reference(and target)genes under *** combining the efficiency calibrated and standard curve methods with the 2−ΔΔCt method,the standard cDNA concentration curve with a logarithmic scale was obtained for each primer pair for each *** a result,an R 2≥0.9999 and the efficiency(E)=100±5% should be achieved for the best primer pair of each gene,which serve as the prerequisite for using the 2^(−ΔΔCt) method for data *** applied our newly developed approach to identify the best reference genes in different tissues and at various inflorescence developmental stages of Tripidium ravennae,an ornamental and biomass grass,and validated their utility under varying abiotic stress *** also applied this approach to test the expression stability of six reference genes in soybean under biotic stress treatment with Xanthomonas axonopodis ***(Xag).Thus,these case studies demonstrated the effectiveness of our optimized protocol for qPCR analysis.

关键词： optimization analysis stepwise

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大豆叶与花形态异常、雌性不育突变体NJS-10H的发现

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大豆科学 2005年第1期24卷 1-4页

作者：赵团结盖钧镒南京农业大学大豆研究所

　在NJCMS1A天然杂交后代中发现突变体NJS-10H。该突变体小叶变窄,叶形态异常,萼片、花瓣数增加,雄蕊减少,雌性不育。雌性不育与叶和花形态异常性状同时出现,受1对隐性基因控制,是一类新的育性异常材料。

关键词：雌性不育花形突变体大豆萼片杂交后代育性形态异常发现天然

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中国大豆育种的核心祖先亲本分析

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南京农业大学学报 2001年第2期24卷 20-23页

作者：盖钧镒赵团结南京农业大学大豆研究所

根据系谱资料归纳出 192 3～ 1995年中国育成的 65 1个大豆品种的 34 8个祖先亲本 ,从中选择对中国大豆育成品种遗传贡献最大的纳入核心祖先亲本名单。其中从东北、黄淮海地区、南方和国外引种 4个子群体入选祖先亲本数分别为2 5 ,2 1,1... 详细信息

根据系谱资料归纳出 192 3～ 1995年中国育成的 65 1个大豆品种的 34 8个祖先亲本 ,从中选择对中国大豆育成品种遗传贡献最大的纳入核心祖先亲本名单。其中从东北、黄淮海地区、南方和国外引种 4个子群体入选祖先亲本数分别为2 5 ,2 1,19和 10。入选的 75份祖先亲本占总数的 2 1 5 5 % ,对 65 1个育成品种的核遗传贡献占 68 99% ,质遗传贡献占72 5 0 %。

关键词：大豆品种核心亲本育种

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大豆2个雌雄不育突变体的发现与鉴定

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大豆科学 2006年第2期25卷 109-112页

作者：赵团结盖钧镒南京农业大学大豆研究所国家大豆改良中心作物遗传与种质创新国家重点实验室南京210095

从(南农73-935×Beeson)F3株行和Co6 0γ射线辐照处理南农87C-38的M5株行分别发现2个育性异常材料NJS-18H、NJS-19H。后裔试验育性鉴定表明NJS-18H、NJS-19H不育株的雌性与雄性育性均不正常,不能正常结荚,可能为联会异常突变体,其不育... 详细信息

从(南农73-935×Beeson)F3株行和Co6 0γ射线辐照处理南农87C-38的M5株行分别发现2个育性异常材料NJS-18H、NJS-19H。后裔试验育性鉴定表明NJS-18H、NJS-19H不育株的雌性与雄性育性均不正常,不能正常结荚,可能为联会异常突变体,其不育性均受1对隐性基因控制。该2个突变体可能用作大豆雌雄配子发育及其相互遗传关系研究的材料。

关键词：大豆突变体雌雄不育性遗传分析

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Genetic diversity,geographic differentiation and evolutionary relationship among ecotypes of Glycine max and G. soja in China

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Chinese Science Bulletin 2009年第21期54卷 4393-4403页

作者： WEN ZiXiang, zhao tuanjie, DING YanLai & GAI JunYi Soybean Research Institute of Nanjing Agricultural University National Center for Soybean Improvement National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China Soybean Research Institute of Nanjing Agricultural University Nanjing 210095 China National Center for Soybean Improvement Nanjing 210095 China National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing 210095 China

The knowledge of origin and evolution of cultivated soybeans is one of the basic issues in both biology and agronomy of the crop. In order to investigate the nuclear and cytoplasmic genetic diversity, geographic differentiation and genetic relationship among geographic ecotypes of cultivated (Glycine max) and wild (G. soja) soybeans, the allelic profiles at 60 nuclear simple-sequence repeat (nuSSR) loci and 11 chloroplastic SSR (cpSSR) loci evenly distributed on whole genome of 393 landraces and 196 wild accessions from nation-wide growing areas in China were analyzed. (i) The genetic diversity of the wild soybean was obviously larger than that of the cultivated soybean, with their nuSSR and cpSSR alleles as 1067 vs. 980 and 57 vs 44, respectively. Of the 980 nuclear alleles detected in the cultivated soybean, 377 new ones (38.5%) emerged, while of the 44 chloroplastic alleles in the cultivated soybean, seven new ones (15.9%) emerged after domestication. (ii) Among the cultivated geographic ecotypes, those from southern China, including South-Central China, Southwest China and South China possessed relatively great genetic diversity than those from northern China, while among the wild geographic ecotypes, the Middle and Lower Changjiang Valleys wild ecotype showed the highest genetic diversity. (iii) The analysis of molecular variance, association analysis between geographic grouping and molecular marker clustering and analysis of specific-present alleles of ecotypes demonstrated that the geographic differentiation of both cultivated and wild soybeans associated with their genetic differentiation, or in other words, had their relevant genetic bases. (iv) The cluster analysis of all accessions clearly showed that the wild accessions from Middle and Lower Changjiang Valleys and South-Central & Southwest China had relatively small genetic distances with all cultivated accessions. The UPGMA dendrogram among geographic ecotypes further showed that the genetic distances b

关键词：地理学遗传多样性异性

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“需求导向、交叉融合”的双蜘蛛网式专业改造模式探索

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文化创新比较研究 2021年第8期5卷 14-16页

作者：赵团结国际教育学院武昌工学院湖北武汉430065

该文在充分调研人才市场需求和兄弟院校的基础上,结合国际教育学院的实际,对学院的传统专业进行了大刀阔斧的改造,确定了“需求导向、交叉融合”的双蜘蛛网式专业改造模式,即通过改造传统专业,建设“学前教育”特色专业,打造“语言(英... 详细信息

该文在充分调研人才市场需求和兄弟院校的基础上,结合国际教育学院的实际,对学院的传统专业进行了大刀阔斧的改造,确定了“需求导向、交叉融合”的双蜘蛛网式专业改造模式,即通过改造传统专业,建设“学前教育”特色专业,打造“语言(英、汉、法、日)+教育(学前、国际、基础、职业)”为骨干学科的,跨越国别、学院、专业的限制,交叉融合产生多个具体的方向,形成稳固、科学、创新的“双蜘蛛网”式专业布局的国际教育学院办学特色。

关键词：传统专业改造语言教育双蜘蛛网式

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中国大豆育成品种中不同地理来源种质的遗传贡献

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中国农业科学 1998年第5期31卷 35-43页

作者：盖钧镒赵团结崔章林邱家驯国家大豆改良中心

在对１９２３～１９９５年育成的６５１个中国大豆品种系谱分析的基础上，计算每一育成品种祖先亲本的细胞核和细胞质遗传贡献值。在全国３４７个细胞核祖先亲本和２１４个细胞质祖先亲本中，来自东北、黄淮海、南方及国外的祖先亲本对... 详细信息

在对１９２３～１９９５年育成的６５１个中国大豆品种系谱分析的基础上，计算每一育成品种祖先亲本的细胞核和细胞质遗传贡献值。在全国３４７个细胞核祖先亲本和２１４个细胞质祖先亲本中，来自东北、黄淮海、南方及国外的祖先亲本对全国育成品种细胞核与细胞质遗传贡献分别为４８．２３％和５０．５４％，２５．５０％和２９．４９％，１３．７４％和１６．２８％，１１．９５％和２．９２％。进一步分析了东北、黄淮海、南方三大生态区大豆育成品种的核、质来源，包括国外引种所占的份额及其特点。评选出３８个对全国和三大生态区域遗传贡献最大的种质

关键词：大豆种质资源遗传贡献

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