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Inhibition of Metalloproteinase Activity by Chinese Formulations Used to Treat Inflammatory Diseases

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Chemical Research in Chinese Universities 2006年第2期22卷 245-247页

作者： JIN Feng-hai WANG Hui-ling zhao shu-hua YANG Jin-gang FANG Xue-xun Key Laboratory of Molecular Enzymology and Engineering the Ministry of Education Jilin University Changchun 130021 P. R. China School of Pharmacy Jilin University Changchun 130021 P. R. China College of Life Science Jilin University Changchun 130021 P. R. China The China-Japan Union Hospital Jilin University Changchun 130021 P. R. China

Matrix metalloproteinases （MMPs） are known to be involved in a number of pathological processes including cancer, atherosclerosis, arthritis and neurodegenerative disease among others. The drive to develop MMP inhibitors as therapeutics has been put forward for years by pharmaceutical companies as well as academicians. In an attempt to screen for MMP inhibitors from Traditional Chinese Medicines （TCMs） , a number of Chinese formulations used to treat inflammatory diseases such as nephritis and hepatitis have been studied. Strong inhibitory effects of three Chinese formulations toward the activity of MMP-16 have been discovered. These results suggest that these anti-inflammatory medicines contain some unknown MMP inhibitory compound（s） and provide reasonable molecular mechanisms for their theraoeutic effects.

关键词： Matrix metalloproteinase (MMP) Matrix metalloproteinase inhibitor ( MMPI ) Traditional Chinese Medicine(TCM) Herbal formulation

Fabrication of Graphene by Cleaving Graphite Chemically

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Chemical Research in Chinese Universities 2011年第4期27卷 547-549页

作者： zhao shu-hua zhao Xiao-ting FAN Hou-gang YANG Li-li ZHANG Yong-jun YANG Jing-hai Physics College of Jilin Normal University Siping 136000 P. R. China

Graphite was chemically cleaved to graphene by Billups Reaction,and the morphologies and microstructures of graphene were characterized by SEM,Raman and *** results show that the graphite was first functionalized by 1-iodododecane,which led to the cleavage of the graphene layer in the *** second decoration cleaved the graphite further and graphene was *** heights of the graphene layer were larger than 1 nm due to the organic decoration.

关键词： Graphene Graphite Chemical cleaving

Catechu Inhibiting Cathepsin B Activity and Motility of HT1080 Cells

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Chemical Research in Chinese Universities 2011年第2期27卷 269-272页

作者： YUAN Hao YANG Jin-gang WANG Zhong-yuan zhao shu-hua YU Da-hai FANG Xue-xun Key Laboratory of Molecular Enzymology and Engineering Ministry of Education College of Life Science Jilin University Changchun 130012 P. R. China Department of Traditional Chinese Medicine China-Japan Union Hospital of Jilin University Changchun 130041 P R. China

We reported that Catechu extract has a significant inhibitory effect on cathepsin B activity. The IC50 value for the Catechu extract against cathepsin B was 7.6 μg/mL. In addition, we showed that HT1080 human fibrosarcoma cells express cathepsin B and Catechu modulate the invasion and motility of these cells. These data may provide molecular mechanisms for the therapeutic effects of Catechu.

关键词： Cathepsin B Traditional Chinese medicine Catechu Tumor Cell invasion Cell motility

Inhibitory Effects of Polygoni Multiflori Caulis Extracts on Matrix Metalloproteinase Activities

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Chemical Research in Chinese Universities 2009年第2期25卷 211-215页

作者： LIU Jun-song YANG Jin-gang HONG Yuan JIN Feng-hai WANG Ming-cui zhao shu-hua FANG Xue-xun Key Laboratory of Molecular Enzymology and Enzyme Engineering of Ministry of Education Jilin University Changchun 130021 P. R. China College of Life Science Jilin University Changchun 130012 P. R. China College of Medicine Jilin University Changchun 130021 P. R. China Department of Traditional Chinese Medicine China-Japan Union Hospital Jilin University Changchun 130033 P. R. China State key Laboratory of Superhard Materials Jilin University Changchun 130012 P. R. China

Matrix metalloproteinases（MMPs） play an essential role in development and tissue remodeling of living organisms. However, the overexpression of MMPs has lead to a series of diseases, such as cancer, arthritis, and atherosclerosis; and inhibition of MMPs may have therapeutic benefits. The discovery of MMP inhibitors from herbal has become a prospective event. We showed that the extract of Polygoni multiflori caulis from ethyl acetate or water（ethyl acetate extract and water extract） can inhibit the activities of MMPs 9, 14, and 16 in a dose-dependent manner and n-butyl alcohol extract of it can also inhibit these MMPs. Furthermore, we found that n-butyl alcohol extract and water extract of it influence the cell viability. These discoveries may contribute to the development of MMP in- hibitors for the therapy of a variety of pathological conditions.

关键词： Matrix metalloproteinases(MMPs) Polygoni multi/lori caulis Water extract Cell viability assay

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从科学发展观看城乡市场主体的统一

中国行政管理 2006年第8期 62-65页

作者：赵淑华哈尔滨理工大学经济管理学院经济系哈尔滨150080

统筹城乡发展需要统一城乡市场,统一城乡市场需要统一城乡市场主体。农村集体经济组织在我国农村有着坚实的社会基础和法律基础,重建农村集体经济组织是构建农村市场主体、实现与城市市场主体对接的关键所在。赋予农村集体经济组织独立... 详细信息

统筹城乡发展需要统一城乡市场,统一城乡市场需要统一城乡市场主体。农村集体经济组织在我国农村有着坚实的社会基础和法律基础,重建农村集体经济组织是构建农村市场主体、实现与城市市场主体对接的关键所在。赋予农村集体经济组织独立的法律人格,令其担负起农村土地等集体财产所有者的重任,可以明晰农村集体产权关系,促进城乡统筹发展战略的落实。

关键词：科学发展观城乡市场农村集体经济组织

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混合型脑囊虫病致智力低下1例

实用儿科临床杂志 2007年第1期22卷 23-23,50页

作者：赵淑华大理学院附属医院儿科云南大理671000

患儿,男,8岁,因失语、智力下降2年,抽搐1年入院.入院查体:神志清楚,不能言语,时有拍手流涎、吮拇指动作,反应迟钝,心肺腹无异常,四肢肌张力稍高,生理反射存在,病理反射未引出.脑脊液、血囊虫抗体均阳性.脑电图中重度广泛异常.

关键词：神经系统囊虫病混合型智力低下儿童

Drp-1、PGC-1α在胎粪吸入综合征新生大鼠肺组织中的作用及其机制

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解放军医学杂志 2024年第3期49卷 265-271页

作者：徐洁莹赵淑华大理大学临床医学院云南大理白族自治州671000 大理大学第一附属医院儿科暨云南省儿科疾病临床医学分中心云南大理白族自治州671000

目的探讨动力相关蛋白1(Drp-1)、过氧化物酶体增殖物激活受体γ共激活因子1-α(PGC-1α)在胎粪吸入综合征(MAS)新生大鼠肺组织中的作用及其机制。方法50只2~3周龄SD新生大鼠随机分为5组(n=10):对照组、模型组与SN50低、中、高浓度组。... 详细信息

目的探讨动力相关蛋白1(Drp-1)、过氧化物酶体增殖物激活受体γ共激活因子1-α(PGC-1α)在胎粪吸入综合征(MAS)新生大鼠肺组织中的作用及其机制。方法50只2~3周龄SD新生大鼠随机分为5组(n=10):对照组、模型组与SN50低、中、高浓度组。对照组气管暴露后,气管内注入2ml/kg生理盐水,其余各组气管内注入2ml/kg的胎粪悬浮液;24h后对照组、模型组不予处理,SN50低、中、高浓度组腹腔注射10、30、60μg/ml浓度SN50各100μl。6h后处死各组大鼠,检测X线胸片、肺大体观、肺湿/干重比(W/D),HE染色观察肺组织病理变化,Westernblotting检测新生大鼠肺组织中NF-κB(p65)、p-NF-κBp65(p-p65)、Drp-1、PGC-1α蛋白的表达变化,免疫组织化学方法观察新生大鼠肺组织中p65、Drp-1、PGC-1α等相关蛋白表达。结果与对照组比较,模型组胸片及大体观可见炎性浸润,W/D、肺损伤病理评分明显升高(P0.05),而SN50中、高浓度组PGC-1α蛋白表达水平明显升高(P0.05)。免疫组化检测结果显示,与对照组比较,模型组p65、Drp-1蛋白表达水平明显升高(P0.05),而SN50中、高浓度组Drp-1蛋白表达水平明显降低(P<0.05),PGC-1α蛋白表达水平明显升高(P<0.05)。结论胎粪吸入可诱导新生大鼠肺组织炎症,其机制可能与增强氧化应激、促进线粒体功能障碍、激活Drp-1/NF-κB信号通路,以及抑制PGC-1α蛋白表达有关。

关键词：胎粪吸入综合征核因子-κB 氧化应激线粒体

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抗肿瘤药物临床试验质量控制体系的建立

中国新药杂志 2014年第13期23卷 1546-1548页

作者：赵淑华江旻北京大学肿瘤医院北京市肿瘤防治研究所国家药物临床试验机构北京100142

目的:为探索抗肿瘤药物临床试验质量的提高,临床试验过程规范化,确保试验数据真实可靠,建立高效质量控制体系的有效方法。我院结合实际工作经验和国家相关法规,通过制定相关SOP,加强对参与研究人员的培训,对临床试验采取全流程管理,建... 详细信息

目的:为探索抗肿瘤药物临床试验质量的提高,临床试验过程规范化,确保试验数据真实可靠,建立高效质量控制体系的有效方法。我院结合实际工作经验和国家相关法规,通过制定相关SOP,加强对参与研究人员的培训,对临床试验采取全流程管理,建立基于临床研究团队、机构质控小组和机构办公室3方面协作的抗肿瘤药物临床试验质量控制体系。新质控体系的建立显著提高了我院抗肿瘤药物临床试验质量。

关键词：抗肿瘤临床试验质量控制

Retroviral-mediated transfer and functional expression of multidrug resistance gene in human placenta mesenchymal stem cells

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Chinese Medical Journal 2008年第9期121卷 800-805页

作者： HAN Li-ying YE Ming-zhu LI Ya-ping WANG Bo-wei WANG Qiang zhao shu-hua LI He-lian Department of Obstetrics and Gynecology Second Hospital Jilin University Changchun Jilin 130041 China Department of Obstetrics and Gynecology Zhongshan Hospital Xiamen University Xiamen Fujian 361004 China Department of Ophthalmology Second Hospital Jilin University Changchun Jilin 130041 China

Background Most of gynecologic malignancies are sensitive to chemotherapy. Myelosuppression is the main dose-related toxicity of many chemotherapeutic drugs. The human multidrug resistance （mdrl） gene is well known for its ability to confer drug resistance. This study aimed to explore the feasibility of expression and resistance of mdrl gene transduction into human placenta mesenchymal stem cells （P-MSCs） by retrovirus vector. Methods Human P-MSCs were isolated from trypsin-digested term placentas, and their immunophenotypes and differentiation potential were evaluated. Human P-MSCs were transduced by reconstructed retroviral vector containing the mdrl gene and green fluorescent protein （GFP） reporter gene. The integration and expression of the mdrl gene were observed indirectly by the expression of GFP, and fluorescence-activated cell sorter was used to evaluate the functional activity of permeability glycoprotein （P-gp） encoded by the mdrl gene. The stimulating test was made in vitro to show pleiotropic drug resistance of transfected cells. Results The isolated, cultured and expanded P-MSCs expressed stem cell markers such as CD29, CD44 and CD73, and showed osteogenic and adipogenic differentiation potentials under appropriate conditions. The expression of P-gp in the non-transfected P-MSCs cells was （0.4±0.1）%, but increased to （28.1±4.7）% after gene transfection （P〈0.01）. And positive staining of P-gp located mainly at cell membrane and cytoplasm. Accumulation and extrusion assays showed that P-gp expressed by the transfected cells had pump-functional activity and could efflux daunomycin out of cells. The analysis of cell survival confirmed that transfected P-MSCs had a characteristic of multidrug resistance with a significant increase in the resistance to anticancer agents. Conclusions Transfer and expression of human mdrl gene mediated by retrovirus vector conferred P-MSCs drug resistance. It might provide a new alternative to chemoprotection strategies.

关键词： drug resistance transfer stem cells placenta