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Draft genome of an Aerophobetes bacterium reveals a facultative lifestyle in deep-sea anaerobic sediments

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Science Bulletin 2016年第15期61卷 1176-1186页

作者： Yong Wang zhao-ming gao Jiang-Tao Li Salim Bougouffa Ren Mao Tian Vladimir B. Bajic Pei-Yuan Qian Sanya Institute of Deep Sea Science and Engineering ChineseAcademy of Sciences Sanya 572000 China Division of Life Science Hong Kong University of Science and Technology Clear Water Bay Hong Kong China State Key Laboratory of Marine Geology Tongji UniversityShanghai 200092 China Computational Bioscience Research Center King Abdullah University of Science and Technology Thuwal Jeddah 23955-6900 Saudi Arabia

Aerophobetes （or CD12） is a recently defined bacterial phylum, of which the metabolic processes and ecological importance remain unclear. In the present study, we obtained the draft genome of an Aerophobetes bac- terium TCSI from saline sediment near the Thuwal cold seep in the Red Sea using a genome binning method. Analysis of 16S rRNA genes of TCS1 and close relatives revealed wide distribution of Aerophobetes in deep-sea sediments. Phylogenetic relationships showed affinity between Aerophobetes TCS 1 and some thermophilic bac- terial phyla. The genome of TCS1 （at least 1.27 Mbp） contains a full set of genes encoding core metabolic path- ways, including glycolysis and pyruvate fermentation to produce acetyl-CoA and acetate. The identification of cross-membrane sugar transporter genes further indicates its potential ability to consume carbohydrates preserved inthe sediment under the microbial mat. Aerophobetes bac- terium TCS1 therefore probably carried out saccharolytic and fermentative metabolism. The genes responsible for autotrophic synthesis of acetyl-CoA via the Wood-Ljung- dahl pathway were also found in the genome. Phylogenetic study of the essential genes for the Wood-Ljungdahl pathway implied relative independence of Aerophobetes bacterium from the known acetogens and methanogens. Compared with genomes of acetogenic bacteria, Aero- phobetes bacterium TCS 1 genome lacks the genes involved in nitrogen metabolism, sulfur metabolism, signal trans- duction and cell motility. The metabolic activities of TCS 1 might depend on geochemical conditions such as supplies of CO2, hydrogen and sugars, and therefore the TCSI might be a facultative bacterium in anaerobic saline sedi- ments near cold seeps.

关键词： Deep-sea sediment Aerophobetes Metagenome Sugar fermentation Cold seep

Enrichment and characterization of ananaerobic cellulolytic microbial consortium SQD-1.1 from mangrove soil

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Enrichment and characterization of ananaerobic cellulolytic ...

Elevated progranulin contributes to synaptic and learning deficit due to loss of fragile X mental retardation protein

福建省海洋学会2014年学术年会暨福建省科协第十四届学术年会

作者： zhao-ming gao Ling-Wei Ruan Xun Xu State Key Laboratory of Marine Environmental Science College of Ocean & Earth Science Xiamen University Key Laboratory of Marine Biogenetic Resources Third Institute of OceanographyState Oceanic Administration (SOA)

Enrichment of microbial consortia provides an approach to simulate and investigate microbial communities in natural environments. In this study, a cellulolytic microbial consortium SQD-1.1 was enriched from mangrove soil of Qinglan port(Hainan, China) by 27-times continuous subcultivation under anaerobic static conditions. The consortium could completely degrade0.2%(w/v) filter paper within three days and utilized it as the sole carbon source. PCR-denaturing gradient gel electrophoresis(DGGE) analysis revealed a stable microbial community structure in the incubation process of 10 days and in the procedure of subcultivation. 24 OTUs belonging to seven phyla were obtained from the full-length 16 S rR NA gene library. Five clones, closest related to the genera Alkaliflexus, Clostridium, Alistipes, Spirochaeta and Trichococcus, were the predominant ones. Among them, M117, phylogeneticly showing high similarity(16S rR NA gene identity, 95.3%) with the cellulolytic anaerobic bacterium Clostridium straminisolvens CSK1 T, was the potential key cellulolytic bacterium. Using the plate cultivation method, twelve strains, including one potential new species and four potential new species of new genera, were isolated. The strainP 2, corresponding to the most frequently detected clone(M05) in the 16 S rR NA gene library, showed both CMCase and xylanase activity and may be another important cellulolytic bacterium. The findings of cellulase activity in cell pellet and cohesion and dockerin domains in metagenome data further suggested the potential of utilization of cellulosome by the consortium to degrade cellulose. Consortium SQD-1.1 provides a candidate for investigating the mechanism of cellulose degradation under anoxic conditions in natural environments.

关键词： microbial consortia cellulolytic bacterium mangrove cellulosome Cellulase

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中国药理学与毒理学杂志 2019年第10期 765-766页

作者： ZHANG Kun LI Yu-jiao GUO Yan-yan ZHENG Kai-yin YANG Qi YANG Le CHEN Tao ZHUO Min zhao ming-gao Department of Pharmacology School of PharmacyFourth Military Medical University Center for Neuron and Disease Frontier Institutes of Life Science and of Science and Technology Xi′an Jiaotong University Department of Anatomy Histology and Embryology and K.K.Leung Brain Research Center Fourth Military Medical University

OBJECTIVE Fragile X syndrome is an inheritable form of intellectual disability caused by loss of fragile X mental retardation protein(FMRP, encoded by the Fmr1 gene). METHODS Four protein level in brain homogenate were detected by ELISA. Morris water maze and novel object(location) were used to study changes of learning and memory. Golgi staining was used to study changes of dendritic spine density. Ed U staining was used to study number of neurogenesis in SGZ. Immunofluorescence staining was used to detect effects of exogenous protein on spine density and morphology. Patch clamp and Med64 electrophysiological technology were used to observe the effects of drugs on synaptic transmission and plasticity. Lentivirus expressing sh RNA was injected in medial prefrontal cortex(m PFC)to study gene function. RESULTS Absence of FMRP caused overexpression of progranulin(PGRN, encoded by Grn), a putative tumour necrosis factor receptor *** the present study, we found that PGRN m RNA and protein were upregulated in the of Fmr1 knock-out *** Fmr1 knock-out mice, elevated PGRN caused insufficient dendritic spine pruning and late-phase long-term potentiation in the m PFC of Fmr1 knock-out mice. Partial PGRN knock down restored spine morphology and reversed behavioural deficits, including impaired fear memory, hyperactivity, and motor inflexibility in Fmr1 knock-out mice. PGRN increased levels of phosphorylated glutamate ionotropic receptor Glu A1 and nuclear factor kappa B(NFκB) in cultured wild-type neurons. Tumour necrosis factor receptor 2(TNFR2) antibody perfusion blocked the effects of progranulin on Glu A1 phosphorylation; this result indicates that TNFR2 is required for PGRN mediated Glu A1 phosphorylation and late-phase long-term potentiation expression. However, high basal level of progranulin in Fmr1 knock-out mice prevented further facilitation of synaptic plasticity by exogenous PGRN. Partial downregulation of PGRN or TNFR2/NFκB signalling restored synaptic p

关键词： fragile X syndrome progranulin TNFR2 long-term potentiation synaptic plasticity

Investigation of bubbles escape behavior from low basicity mold flux for high-Mn high-Al steels using 3D X-ray microscope

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 102-110页

作者： Qiang Liu Xiang Li Shen Du ming gao Yanbin Yin Jiongming Zhang State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China

During the continuous casting process of high-Mn high-Al steels,various types of gases such as Ar need to escape through the top of the *** which,the behavior of bubbles traversing the liquid slag serves as a restrictive link,closely associated with viscosity and the thickness of liquid *** contrast to two-dimensional surface observation,three-dimensional(3D)analysis method can offer a more intuitive,accurate,and comprehensive ***,this study employs a 3D X-ray microscope(3D-XRM)to obtained spatial distribution and 3D morphological characteristics of residual bubbles in mold flux under different basicity of liquid slag,different temperatures,and different holding *** results indicate that as basicity of slag increases from 0.52 to 1.03,temperature increases from 1423 to 1573 K,the viscosity of slag decreases,the floating rate of bubbles *** addition,when holding time increases from 10 to 30 s,the bubbles floating distance increases,and the volume fraction and average equivalent sphere diameter of the bubbles solidified in the mold flux gradually *** one word,increasing the basicity,temperature,and holding time leading to an increase in the removal rate of bubbles especially for the *** findings of bubbles escape behavior provide valuable insights into optimizing low basicity mold flux for high-Mn high-Al steels.

关键词： mold flux low basicity bubbles three-dimensional X-ray microscope viscosity

基于Fibonacci序列准周期结构的小角度低通空间滤波器

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光子学报 2017年第2期46卷 189-196页

作者：陈敏周宏敏罗朝明万婷高明盛湖南理工学院信息与通信工程学院湖南岳阳414000

利用常规材料构造了Fibonacci序列准周期结构,运用传输矩阵法研究了该结构的空间传输特性,并基于该结构优良的空间传输特性设计了小角度低通空间滤波器.数值模拟结果表明,该小角度空间滤波器的角域带宽可通过改变序列的结构类型和序列... 详细信息

利用常规材料构造了Fibonacci序列准周期结构,运用传输矩阵法研究了该结构的空间传输特性,并基于该结构优良的空间传输特性设计了小角度低通空间滤波器.数值模拟结果表明,该小角度空间滤波器的角域带宽可通过改变序列的结构类型和序列数来调谐,其调谐规律为:随着Fibonacci序列F(m,1)中m值的增加,对应空间滤波器的角域带宽减小;随着序列数的增大,对应角域带宽也减小.在调谐的基础上,还可通过改变构成准周期结构的介质折射率参量来精确调节其角域带宽.相比于基于超材料的小角度空间滤波器而言,基于Fibonacci序列的小角度空间滤波器制备更简单,且有望应用于新一代的高功率激光系统中.

关键词：信息光学空间滤波器传输矩阵法 Fibonacci序列准周期结构小角域带宽透射谱

Low-cost and fluoride-free synthesis of MFI zeolite nanosheets with enhanced stability for benzene alkylation with ethanol

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Journal of Energy Chemistry 2025年第1期100卷 458-468页

作者： Peng Zhu Cun Liu Yiren Yu Guoshu gao Yumeng zhao Xiongfu Zhang Guodong Liu Guohui Yang State Key Laboratory of Fine Chemicals School of Chemical Engineering Dalian University of Technology State Key Laboratory of Catalysis Dalian Institute of Chemical Physics Chinese Academy of Sciences

Zeolite nanosheets with a short b-axis thickness are highly desirable in lots of catalytic reactions due to their reduced diffusion resistance. Nevertheless, conventional synthesis methods usually require expensive structure-directing agents(SDAs), pricey raw materials, and eco-unfriendly fluorine-containing additives. Here, we contributed a cost-effective and fluoride-free synthesis method for synthesizing high-quality MFI zeolite nanosheets through a Silicalite-1(Sil-1) seed suspension and urea cooperative strategy, only with inexpensive colloidal silica as the Si source. Our approach was effective for synthesizing both Sil-1 and aluminum-containing ZSM-5 nanosheets. By optimizing key synthesis parameters,including seed aging time, seed quantity, and urea concentration, we achieved precise control over the crystal face aspect ratio and b-axis thickness. We also revealed a non-classical oriented nanosheet growth mechanism, where Sil-1 seeds induced the formation of quasi-ordered precursor particles, and the(010)crystal planes of these particles facilitated urea adsorption, thereby promoting c-axis-oriented *** obtained ZSM-5 nanosheets exhibited exceptional catalytic performance in the benzene alkylation with ethanol, maintaining stability for over 500 h, which is 5 times longer than traditional ZSM-5 catalysts. Furthermore, large-scale production of ZSM-5 nanosheets was successfully carried out in a 3 L highpressure autoclave, yielding samples consistent with those from laboratory-scale synthesis. This work marks a significant step forward in the sustainable and efficient production of MFI nanosheets using inexpensive and environmentally friendly raw materials, offering the broad applicability in catalysis.

关键词： Aspect ratio

Effects of tumor necrosis factor-alpha on calcium movement in rat ventricular myocytes

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Acta Pharmacologica Sinica 2003年第12期 50-56页

作者： LI Xiao-Qiang, zhao ming-gao, MEI Qi-Bing~2, ZHANG Yan-Feng CAO Wei, WANG Hai-Fang, CHEN Dan~3, CUI Yi Department of Pharmacology, ~3Electron Microscopy Center, The Fourth Military Medical University, Xi-an 710032, China

AIM: To study the effects of tumor necrosis factor-alpha (TNF-α) on calcium movement in rat ventricular myocytes. METHODS: Intracellular free Ca2+concentration was measured with calcium fluorescent probe Fluo-3/AM and laser confocal microscope. L-type calcium current (ICa.L) was recorded with the whole-cell configuration of the patch-clamp techniques. RESULTS: At 2, 20 and 200μg/L, TNF-α was found to increase intracellular free Ca2+concentration in a dose-dependent manner illustrated by the increment of calcium fluorescence density with laser confocal microscope. Nicardipine 0.5μtmol/L slightly attenuated TNF-α-induced response. When the cardiac myocytes were exposed to caffeine (100mmol/L) for 30 rain, TNF-α failed to induce any change of intracellular free calcium. However, it was found that TNF-α inhibited *** whole-cell patch-clamp experiments. At 2, 20, and 200μg/L, TNF-α decreased peak *** 3.9％ (-5.1 pA/pF±0.3 pA/pF vs -4.9 pA/pF±0.2 pA/pF, n=9, P>0.05), 15.7

关键词： tumor necrosis factor myocardium calcium: patch-clamp techniques confocal microscopy

Deficits in LTP facilitation by 5-HT2A receptor antagonist in a mouse model for fragile X syndrome

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Deficits in LTP facilitation by 5-HT2A receptor antagonist i...

CB1 agonism prolongs time window for estrogen replacement therapy

中国药理学会第十一次全国学术会议

作者： XU zhao-hui,MA Lan,zhao ming-gao (Department of Pharmacology,School of Pharmacy,Fourth Military Medical University,Xi’an 710032,China)

Fragile X syndrome(FXS) is a common inherited mental retardation caused by the lack of fragile X mental retardation protein(FMRP) due to silencing of the Fmr1 ***,we report that FMRP is involved in serotonin(5-HT) modulation of synaptic *** inhibition of 5-HT2A receptor(5-HT2AR) by R-96544 facilitated the induction of long-term potentiation (LTP) in the anterior cingulate cortex(ACC) of the wild-type(WT) *** facilitation of LTP by R-96544 depended on the activation of NMDARs and elevated postsynaptic Ca concentrations,indicating the postsynaptic mechanism underlying the LTP induction. However,the LTP facilitation by R-96544 was reduced in the ACC of the Fmr1 knock-out(KO) ***,AMPA receptor subtype 1(GluR1) surface expression in response to 5-HT2AR inhibition was impaired in the cultured ACC neurons from Fmr1 KO ***,these findings suggest that FMRP is involved in serotonin receptor signaling and contributes to GluR1 surface expression induced by 5-HT2AR inactivation.

关键词： serotonin fragile X syndrome synaptic plasticity anterior cingulate cortex

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中国药理学与毒理学杂志 2018年第9期32卷 683-684页

作者： ZHANG Kun LI Yan-jiao YANG Qi LI Yu-jiao YANG Le WANG Xin-shang DANG Rui-li GUAN Shao-yu GUO Yan-yan GE Xiang-wei SUN Ting WU Yu-mei LIU Shui-bing zhao ming-gao Precision Pharmacy & Drug Development Center Department of Pharmacy Tangdu Hospital the Fourth Military Medical University Xi′an 710038 China′a710038China Institute of Clinical Pharmacy and Pharmacology Jining First People′ s Hospital Jining

OBJECTIVE To detect the underlying mechanism of time window for estrogen(E2)replacement treating cognitive *** E2 begun 1 week after the ovariectomy(OVXST)or 3 months after the ovariectomy(OVXLT).Learning and memory ability were examined by trace fear memory test and inhibitory avoidance *** and LTD were detected by *** throughput gene expression sequencing and microRNA(miR NA) sequencing were used to detecte the differently expressed genes between OVXSTand OVXLTafter estrogen *** Subcutaneous injection of E2 improved fear memory formation in both 1 week after ovariectomy(OVXST) mice or 3 months after ovariectomy(OVXLT) ***,for fear memory extinction,facilitated by E2 in OVXSTmice,but impaired by E2 in *** researches showed in medial prefrontal cortex(mPFC),estrogen facilitates LTD in OVXSTmice but impairs LTD in *** of highthroughput sequencings of mR NA and miRNA in mPFC from sham,OVXSTmice,E2 treated OVXST mice,OVXLTmice,and E2 treated OVXLTmice indicated decreased miR-221-5 p expression in OVXLTmice compared with *** OVXLT mice,miR-221-5 p could be further reduced by E2 ***,miR-221-5 p targeted neuralized E3 ubiquitin protein ligase 1 a/b(Neurl1 a/b) m *** miR-221-5 p will promotes cannabinoid receptor 1(CB1) ubiquitination through up-regulating Neurl1 a/b protein levels in E2 treated OVXLTmice,which disrupted the retrograde endocanabinoids *** miR-221-5 p or treating with CB1 agonist rescued the fear extinction impairment in E2 treated *** These results uncovered a epigenetic change after long term E2 responsible for failure of E2 improving cognitive performance in OVXLTmice,moreover miR-221-5 p and CB1 agonist as potential targets for prolonging the time window for E2 replacement therapy.

关键词： cannabinoid receptor1 estrogen time window miRNA epigenetic