检索结果-南通市图书馆

Nanobodies against African swine fever virus p72 and CD2v proteins as reagents for developing two cELISAs to detect viral antibodies

引用

Virologica Sinica 2024年第3期39卷 478-489页

作者： Jiahong Zhu Qingyuan liu liuya Li Runyu Zhang Yueting Chang Jiakai zhao Siyu liu Xinyu zhao Xu Chen Yani Sun Qin zhao department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F UniversityYangling Observing and Experimental Station of National Data Center of Animal HealthMinistry of AgricultureYangling712100China

African swine fever virus(ASFV)poses a significant threat to the global swine ***,there are no effective vaccines or treatments available to combat ASFV infection in *** primary means of controlling the spread of the disease is through rapid detection and subsequent elimination of infected ***,a lower virulent ASFV isolate with a deleted EP402R gene(CD2v-deleted)has been reported in china,which further complicates the control of ASFV infection in pig ***,an EP402R-deleted ASFV variant has been developed as a potential live attenuated vaccine candidate ***,it is crucial to develop detection methods that can distinguish wild-type and EP402R-deleted ASFV *** this study,two recombinant ASFV-p72 and-CD2v proteins were expressed using a prokaryotic system and used to immunize Bactrian ***,eight nanobodies against ASFV-p72 and ten nanobodies against ASFV-CD2v were *** the production of these nanobodies with horse radish peroxidase(HRP)fusion proteins,the ASFV-p72-Nb2-HRP and ASFV-CD2v-Nb22-HRP fusions were selected for the development of two competitive ELISAs(cELISAs)to detect anti-ASFV *** two cELISAs exhibited high sensitivity,good specificity,repeatability,and *** coincidence rate between the two cELISAs and commercial ELISA kits was 98.6%and 97.6%,***,the two cELISA for detecting antibodies against ASFV demonstrated ease of operation,a low cost,and a simple production *** two cELISAs could determine whether pigs were infected with wild-type or CD2v-deleted ASFV,and could play an important role in monitoring ASFV infections in pig farms.

关键词： African swine fever virus(ASFV) ASFV-p72 ASFV-CD2v Nanobody-HRP Competitive ELISA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pathogenic genes associated with Parkinson’s disease:molecular mechanism overview

引用

BIOCELL 2024年第5期48卷 707-729页

作者： TINGTING liu YIWEI HAO LIFENG zhao School of Life Sciences Institute for Brain Sciences ResearchHenan UniversityKaifeng475004China Pharmaceutical department Peking University Cancer Hospital Inner Mongolia HospitalHohhot010010China

Parkinson’s disease(PD)is a common neurodegenerative disease in the elderly,accounting for more than 1%of the population aged 65 *** inheritance is relatively rare in PD,accounting for approximately 5%to 10%of PD patients,and there is a growing body of evidence suggesting that multiple genetic risk factors play a significant role in the pathogenesis of *** groups have identified and reported a number of genes carrying mutations associated with affected family *** genes associated with PD are also candidates for idiopathic PD,and these genes may also carry other mutation sites that increase *** multiple genetic risk factors are combined,the risk of PD is increased to a greater extent,and to unravel the pathogenic pathways that lead to different forms of *** review focuses on the association of PD genes,such as Parkinson Disease 1-24(PARK1-24),glucosylceramidase(GBA),GTP cyclohydrolase 1(GCH1),fibroblast growth factor 20(FGF20),nuclear receptor-related factor 1(NURR1),NUS1 dehydrodolichyl diphosphate synthase subunit(NUS1),diacylglycerol Lipase Beta(DAGLB),transmembrane protein(TMEM),ubiquinol-cytochrome c reductase core protein 1(UQCRC1),glycoprotein non-metastatic melanoma protein B protein(GPNMB),dynactin 1(DCTN1),LDL receptor related protein 10(LRP10),monoamine oxidase(MAO),ataxin 2(ATXN2),microtubule associated protein tau(MAPT),pantothenate kinase 2(PANK2),spastic parapplegia type 11(SPG11),polymer gamma(POLG),TATA-box binding protein associated factor 1(TAF1),dual specificity tyrosine phosphorylation regulated kinase 1A(Dyrk1a),and crystallin alpha A(CRYAA),with the pathogenesis of *** introduce what is currently known about the molecular genetics of PD to help explain the molecular mechanisms leading to the neurodegenerative disease.

关键词： Parkinson’s disease(PD) Molecular genetics Mutation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Shallow Convection Dataset Simulated by Three Different Large Eddy Models

引用

Advances in Atmospheric Sciences 2024年第4期41卷 754-766页

作者： Yaxin zhao Xiaocong WANG Yimin liu Guoxiong WU Yanjie liu College of Earth and Planetary Sciences University of Chinese Academy of Sciences(UCAS)Beijing 100029China State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics(LASG) Institute of Atmospheric Physics(IAP)Chinese Academy of Sciences(CAS)Beijing 100029China

Shallow convection plays an important role in transporting heat and moisture from the near-surface to higher altitudes,yet its parameterization in numerical models remains a great challenge,partly due to the lack of high-resolution *** study describes a large eddy simulation(LES)dataset for four shallow convection cases that differ primarily in inversion strength,which can be used as a surrogate for real *** reduce the uncertainty in LES modeling,three different large eddy models were used,including SAM(System for Atmospheric Modeling),WRF(Weather Research and Forecasting model),and *** show that the different models generally exhibit similar behavior for each shallow convection case,despite some differences in the details of the convective *** addition to grid-averaged fields,conditionally sampled variables,such as in-cloud moisture and vertical velocity,are also provided,which are indispensable for calculation of the entrainment/detrainment *** the essentiality of the entraining/detraining process in the parameterization of cumulus convection,the dataset presented in this study is potentially useful for validation and improvement of the parameterization of shallow convection.

关键词： large eddy simulation SAM WRF UCLA-LES shallow convection entraining process

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

In Situ Growth of 2D Metal–Organic Framework Ion Sieve Interphase for Reversible Zinc Anodes

引用

Energy & Environmental Materials 2024年第6期7卷 158-166页

作者： Jing Sun Qinping Jian Bin liu Pengzhu Lin Tianshou zhao department of Mechanical and Aerospace Engineering The Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong SARChina department of Mechanical and Energy Engineering Southern University of Science and TechnologyShenzhen 518055China

Zinc metal anodes are gaining popularity in aqueous electrochemical energy storage systems for their high safety,cost-effectiveness,and high ***,the service life of zinc metal anodes is severely constrained by critical challenges,including dendrites,water-induced hydrogen evolution,and *** this study,a protective two-dimensional metal–organic framework interphase is in situ constructed on the zinc anode surface with a novel gel vapor deposition *** ultrathin interphase layer(~1μm)is made of layer-stacking 2D nanosheets with angstrom-level pores of around 2.1Å,which serves as an ion sieve to reject large solvent–ion pairs while homogenizes the transport of partially desolvated zinc ions,contributing to a uniform and highly reversible zinc *** the shielding of the interphase layer,an ultra-stable zinc plating/stripping is achieved in symmetric cells with cycling over 1000 h at 0.5 mA cm−2 and~700 h at 1 mA cm^(−2),far exceeding that of the bare zinc anodes(250 and 70 h).Furthermore,as a proof-of-concept demonstration,the full cell paired with MnO_(2) cathode demonstrates improved rate performances and stable cycling(1200 cycles at 1 A g−1).This work provides fresh insights into interphase design to promote the performance of zinc metal anodes.

关键词： 2D MOF desolvation interphase ion sieve zinc anode

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asperuloside Promotes Apoptosis of Cervical Cancer Cells through Endoplasmic Reticulum Stress-Mitochondrial Pathway

引用

Chinese Journal of Integrative Medicine 2024年第1期30卷 34-41页

作者： QI Zhi-min WANG Xia liu Xia zhao Juan Medical Examination Center Hebei Province Hospital of Chinese MedicineShijiazhuang(050011)China department of Gynaecology and Obstetrics Hebei Province Hospital of Chinese MedicineShijiazhuang(050011)China Experimental Center for Teaching Hebei Medical UniversityShijiazhuang(050017)China

Objective: To investigate the effects of asperuloside on cervical cancer based on endoplasmic reticulum(ER) stress and mitochondrial pathway. Methods: Different doses(12.5–800 μg/mL) of asperuloside were used to treat cervical cancer cell lines Hela and CaSki to calculate the half maximal inhibitory concentration(IC_(50)) of asperuloside. The cell proliferation was analyzed by clone formation assay. Cell apoptosis, intracellular reactive oxygen species(ROS) and mitochondrial membrane potential were determined by flow cytometry. The protein expressions of cleaved-caspase-3, Bcl-2, Bax, Cyt-c, cleaved-caspase-4 and glucose-regulated protein 78(GRP78) were analyzed by Western blot. And the inhibitor of ER stress, 4-phenyl butyric acid(4-PBA) was used to treat cervical cancer cells to further verify the role of ER stress in the apoptosis of cervical cancer cells induced by asperuloside. Results: Asperuloside of 325, 650, and 1300 μg/mL significantly inhibited the proliferation and promoted apoptosis of Hela and CaSki cells(P<0.01). All doses of asperuloside significantly increased intracellular ROS levels, reduced mitochondrial membrane potential, significantly reduced Bcl-2 protein expression level, and increased Bax, Cyt-c, GRP78 and cleaved-caspase-4 expressions(P<0.01). In addition, 10 mmol/L 4-PBA treatment significantly promoted cell proliferation and reduced apoptosis(P<0.05), and 650 μg/mL asperuloside could reverse 4-PBA-induced increased cell proliferation, decreased apoptosis and cleaved-caspase-3,-4 and GRP78 protein expressions(P<0.05). Conclusion: Our study revealed the role of asperuloside in cervical cancer, suggesting that asperuloside promotes apoptosis of cervical cancer cells through ER stress-mitochondrial pathway.

关键词： asperuloside Chinese medicine cervical cancer endoplasmic reticulum stress mitochondrial damage apoptosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Solar manipulations of perpendicular magnetic anisotropy for flexible spintronics

引用

Frontiers of physics 2024年第4期19卷 73-79页

作者： Zhexi He Yifan zhao Yujing Du Meng zhao Yuxuan Jiang Ming liu Ziyao Zhou State Key Laboratory for Manufacturing Systems Engineering Electronic Materials Research LaboratoryKey Laboratory of the Ministry of Education&International Center for Dielectric ResearchSchool of Electronic Science and EngineeringThe International Joint Laboratory for Micro/Nano Manufacturing and Measurement TechnologyXi’an Jiaotong UniversityXi’an 710049China School of Materials Science&Engineering Changzhou UniversityChangzhou 213164China

Flexible electronics/spintronics attracts researchers’attention for their application potential abroad in wearable devices,healthcare,and other *** devices’performance(speed,energy consumption)is highly dependent on manipulating information bits(spin-orientation in flexible spintronics).In this work,we established an organic photovoltaic(OPV)/ZnO/Pt/Co/Pt heterostructure on flexible PET substrates with perpendicular magnetic anisotropy(PMA).Under sunlight illumination,the photoelectrons generated from the OPV layer transfer into the PMA heterostructure,then they reduce the PMA strength by enhancing the interfacial Rashba field *** coercive field(Hc)reduces from 800 Oe to 500 Oe at its maximum,and the magnetization can be switched up and down *** stability of sunlight control of magnetization reversal under various bending conditions is also tested for flexible spintronic ***,the voltage output of sunlight-driven PMA is achieved in our prototype device,exhibiting an excellent angular dependence and opening a door towards solar-driven flexible spintronics with much lower energy consumption.

关键词： interfacial magnetoelectric coupling perpendicular magnetic anisotropy deterministic magnetization reversal photovoltaic control of magnetism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Heat stress affects mammary metabolism by influencing the plasma flow to the glands

引用

Journal of Animal Science and Biotechnology 2024年第5期15卷 1908-1917页

作者： Jia Zeng Diming Wang Huizeng Sun Hongyun liu Feng‑Qi zhao Jianxin liu Institute of Dairy Science College of Animal SciencesZhejiang UniversityHangzhouChina Zhejiang Key Laboratory of Dairy Cow Genetic Improvement and Milk Quality Research Zhejiang UniversityHangzhouChina department of Animal&Veterinary Sciences University of VermontBurlingtonVTUSA

Background Environmental heat stress(HS)can have detrimental effects on milk production by compromising the mammary *** plasma flow(MPF)plays a crucial role in nutrient supply and uptake in the mam-mary *** this experiment,we investigated the physiological and metabolic changes in high-yielding cows exposed to different degrees of HS:no HS with thermal-humidity index(THI)below 68(No-HS),mild HS(Mild-HS,68≤THI≤79),and moderate HS(Mod-HS,79

关键词： Dairy cow Heat stress Mammary metabolism Mammary plasma flow

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transmit Power Minimization for IRS-Assisted NOMA-UAV Networks

引用

china Communications 2024年第5期21卷 137-152页

作者： zhao Chen Pang Xiaowei Tang Jie liu Mingqian zhao Nan Zhang Xiuyin Wang Xianbin School of Information and Communication Engineering Dalian University of TechnologyDalian 116024China School of Electronic and Information Engineering South China University of TechnologyGuangzhou 510641China State Key Laboratory of Integrated Services Networks Xidian UniversityXi’an 710071China department of Electrical and Computer Engineering Western UniversityLondonON N6A 5B9Canada

The flexibility of unmanned aerial vehicles(UAVs)allows them to be quickly deployed to support ground *** reflecting surface(IRS)can reflect the incident signal and form passive beamforming to enhance the signal in the specific *** by the promising benefits of both technologies,we consider a new scenario in this paper where a UAV uses non-orthogonal multiple access to serve multiple users with *** to their distance to the UAV,the users are divided into the close users and remote *** UAV hovers above the close users due to their higher rate requirement,while the IRS is deployed near the remote users to enhance their received *** aim at minimizing the transmit power of UAV by jointly optimizing the beamforming of UAV and the phase shift of IRS while ensuring the decoding ***,the problem is ***,we decompose it into two sub-problems,including the transmit beamforming optimization and phase shift optimization,which are transformed into second-order cone programming and semidefinite programming,*** propose an iterative algorithm to solve the two sub-problems *** results prove the effectiveness of the proposed scheme in minimizing the transmit power of UAV.

关键词： intelligent reflecting surface nonorthogonal multiple access phase shift optimization transmit beamforming optimization unmanned aerial vehicle

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Advancing brain-inspired computing with hybrid neural networks

引用

National Science Review 2024年第5期11卷 56-71页

作者： Faqiang liu Hao Zheng Songchen Ma Weihao Zhang Xue liu Yansong Chua Luping Shi Rong zhao Center for Brain-Inspired Computing Research Optical Memory National Engineering Research CenterTsinghua University-China Electronics Technology HIK Group Co.Joint Research Center for Brain-inspired ComputingIDGMc Govern Institute for Brain Research Department of Precision Instrument Tsinghua University Neuromorphic Computing Laboratory China Nanhu Academy of Electronics and Information Technology

Brain-inspired computing, drawing inspiration from the fundamental structure and information-processing mechanisms of the human brain, has gained significant momentum in recent years. It has emerged as a research paradigm centered on brain–computer dual-driven and multi-network integration. One noteworthy instance of this paradigm is the hybrid neural network(HNN), which integrates computer-science-oriented artificial neural networks(ANNs) with neuroscience-oriented spiking neural networks(SNNs). HNNs exhibit distinct advantages in various intelligent tasks, including perception, cognition and learning. This paper presents a comprehensive review of HNNs with an emphasis on their origin, concepts, biological perspective, construction framework and supporting systems. Furthermore, insights and suggestions for potential research directions are provided aiming to propel the advancement of the HNN paradigm.

关键词： brain-inspired computing hybrid neural network dual-brain driven multi-network integration neuromorphic system

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Application of CRISPR/Cas12i.3 for targeted mutagenesis in broomcorn millet(Panicum miliaceum L.)

引用

Journal of Integrative Plant Biology 2024年第8期66卷 1544-1547页

作者： Yuhe Bai Shengnan liu Yan Bai Zhisong Xu Hainan zhao Haiming zhao Jinsheng Lai Ya liu Weibin Song State Key Laboratory of Maize Bio-breeding Key Laboratory of Genome Editing Research and ApplicationMinistry of Agriculture and Rural AffairsDepartment of Plant Genetics and BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China National Agricultural Technology Extension&Service Center Beijing 100125China Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

As the global human population continues to grow and is predicted to reach 10 billion by 2050,the demand for food volume and quality raises a tremendous challenge(OECD/FAO,2017).The worldwide arable land area is decre... 详细信息

关键词： arable continue raise

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：