检索结果-南通市图书馆

β-谷甾醇对结肠癌细胞HCT116增殖和凋亡的影响

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

中国现代医生 2024年第9期62卷 78-81,85页

作者：陈喜李若男李晶赵李娜徐志立辽宁中医药大学药学院辽宁大连1166001

目的探究β-谷甾醇对结肠癌细胞HCT116增殖和凋亡的影响及其对磷脂酰肌醇3激酶(phosphoinositide 3-kinase,PI3K)/蛋白激酶B(protein kinase B,PKB,又称Akt)信号通路的调控作用。方法体外培养结肠癌细胞HCT116并分为β-谷甾醇高(240μmo... 详细信息

目的探究β-谷甾醇对结肠癌细胞HCT116增殖和凋亡的影响及其对磷脂酰肌醇3激酶(phosphoinositide 3-kinase,PI3K)/蛋白激酶B(protein kinase B,PKB,又称Akt)信号通路的调控作用。方法体外培养结肠癌细胞HCT116并分为β-谷甾醇高(240μmol/L)、中(120μmol/L)、低剂量(60μmol/L)组,设置空白对照组(0μmol/L)。应用不同浓度的β-谷甾醇处理HCT116细胞。24h后应用细胞计数试剂盒8(cell counting kit-8,CCK-8)法检测β-谷甾醇对HCT116细胞增殖的影响,并在显微镜下观察细胞的形态变化;应用Hoechst 33342细胞核染色法检测β-谷甾醇对HCT116细胞凋亡的影响;应用细胞克隆集落形成实验检测HCT116细胞的集落形成能力;应用蛋白质印迹技术检测HCT116细胞中PI3K、磷酸化Akt、Akt、抗凋亡蛋白Bcl-2、促凋亡蛋白Bax等相关蛋白的表达情况;利用AutoDock软件实现β-谷甾醇与PI3K和Akt蛋白的分子对接。结果与对照组比较,β-谷甾醇可浓度依赖性地抑制结肠癌细胞HCT116的增殖,抑制细胞的集落形成能力,促进细胞的凋亡;抑制HCT116细胞中磷酸化Akt、PI3K、Bcl-2蛋白的表达,促进Bax蛋白的表达;β-谷甾醇与PI3K、Akt蛋白的结合均较为稳定。结论β-谷甾醇可通过抑制PI3K/Akt信号通路调节HCT116细胞的增殖与凋亡。

关键词： β-谷甾醇结肠癌增殖凋亡信号通路

在线全文

学校读者我要写书评

暂无评论

贝诺酯合成工艺优化

山东化工 2024年第14期53卷 33-37页

作者：赵莉娜刘方蕊李曼王智贤曹江营山东中医药大学药学院山东济南250355

以乙酰水杨酸为原料,氯化亚砜为氯代试剂,吡啶为催化剂,制得乙酰水杨酰氯。四氢呋喃为溶解乙酰水杨酰氯溶剂,TBAB为相转移催化剂,在5%氢氧化钠碱性条件下,对乙酰氨基酚与乙酰水杨酰氯缩合60 min,得到贝诺酯。对乙酰氨基酚和乙酰水杨酸... 详细信息

以乙酰水杨酸为原料,氯化亚砜为氯代试剂,吡啶为催化剂,制得乙酰水杨酰氯。四氢呋喃为溶解乙酰水杨酰氯溶剂,TBAB为相转移催化剂,在5%氢氧化钠碱性条件下,对乙酰氨基酚与乙酰水杨酰氯缩合60 min,得到贝诺酯。对乙酰氨基酚和乙酰水杨酸的物质的量之比为1∶1.2。此工艺路线温和,后处理和纯化简单。总收率为69%。所得产物经过1H-NMR和13 C-NMR确证结构。

关键词：贝诺酯合成优化相转移催化剂

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

陀螺状碳酸钙的“一步法”制备及性能表征

山东化工 2024年第8期53卷 14-17页

作者：宋晓阳王禹心杨清华赵丽娜吉林师范大学化学学院吉林四平136000

以维生素B_(3)为改性剂,在水溶液体系利用“一步法”制备了陀螺状碳酸钙粒子。采用控制变量法考察了反应温度和改性剂添加量等因素对碳酸钙结晶行为的影响,并确定最佳反应条件。利用SEM、XRD对产物的结构和性能进行表征,并对成核机理进... 详细信息

以维生素B_(3)为改性剂,在水溶液体系利用“一步法”制备了陀螺状碳酸钙粒子。采用控制变量法考察了反应温度和改性剂添加量等因素对碳酸钙结晶行为的影响,并确定最佳反应条件。利用SEM、XRD对产物的结构和性能进行表征,并对成核机理进行初步探讨。此方法对碳酸钙的改性研究具备一定指导意义。

关键词：陀螺状维生素B_(3)(烟酸,NA) 碳酸钙一步法

养护条件对混凝土力学性能及抗硫酸盐侵蚀性能的影响

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

混凝土与水泥制品 2024年第7期 38-41页

作者：曹建青张萌赵丽娜范文晓令芳新疆工程学院新疆乌鲁木齐830091 新疆大学建筑工程学院新疆乌鲁木齐830017

针对新疆盐渍环境及高温干旱气候条件下混凝土普遍存在的硫酸盐侵蚀问题,研究了不同养护条件[标准养护、自然条件下内养护、(40±2)℃恒温内养护]对混凝土力学及抗硫酸盐侵蚀性能的影响,进行了混凝土的抗压强度、干湿循环硫酸盐侵蚀试验... 详细信息

针对新疆盐渍环境及高温干旱气候条件下混凝土普遍存在的硫酸盐侵蚀问题,研究了不同养护条件[标准养护、自然条件下内养护、(40±2)℃恒温内养护]对混凝土力学及抗硫酸盐侵蚀性能的影响,进行了混凝土的抗压强度、干湿循环硫酸盐侵蚀试验,计算了质量损失率及耐蚀系数。结果表明:SAP内养护可以在一定程度上改善混凝土的抗压强度,且可以延缓干湿循环硫酸盐侵蚀对混凝土的腐蚀破坏;混凝土的耐蚀系数与质量损失率之间有显著的负相关性,随着耐蚀系数的降低,混凝土的质量损失率增加。

关键词：养护条件力学性能硫酸盐侵蚀干湿循环混凝土

同方期刊数据库

Bubble domain evolution in well-ordered BiFeO_(3) nanocapacitors

在线全文

学校读者我要写书评

暂无评论

Journal of Advanced Dielectrics 2023年第6期13卷 13-19页

作者： Fengyuan Zhang Guo Tian lina zhao Xingsen Gao Jiangyu Li Department of Materials Science and Engineering Southern University of Science and Technology Shenzhen 518055P.R.China Guangdong Provincial Key Laboratory of Functional Oxide Materials and Devices Southern University of Science and Technology Shenzhen 518055P.R.China Institute for Advanced Materials South China Academy of Advanced Optoelectronics South China Normal UniversityGuangzhou 510006P.R.China Guangdong Provincial Key Laboratory of Optical Information Materials and Technology South China Normal University Guangzhou 510006P.R.China Institute of Data and Information Tsinghua Shenzhen International Graduate School Shenzhen 518055P.R.China

Ferroelectric nanocapacitors have attracted intensive research interest due to their novel functionalities and potential application in ***,due to the lack of knowledge of domain evolution in isolated nanocapacitors,precise manipulation of topological domain switching in the nanocapacitor is still a ***,we report unique bubble and cylindrical domains in the well-ordered BiFeO_(3) nanocapacitor *** transformation of bubble,cylindrical and mono domains in isolated ferroelectric nanocapacitor has been demonstrated via scanning probe microscopy(SPM).The bubble domain can be erased to mono domain or written to cylindrical domain and mono domain by positive and negative voltage,***,the domain evolution rules,which are mainly affected by the depolarization field,have been observed in the nanocapacitors with different domain *** work will be helpful in understanding the domain evolution in ferroelectric nanocapacitors and providing guidance on the manipulation of nanoscale topological domains.

关键词： Ferroelectrics SPM domain evolution bubble domain nanocapacitor array

ZmMs33 promotes anther elongation via modulating cell elongation regulators,metabolic homeostasis,and cell wall remodeling in maize

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Crop Journal 2023年第2期11卷 361-373页

作者： Ziwen Li Taotao Zhu Shuangshuang Liu lina zhao Xueli An Yan Long Xun Wei Juan Zhang Zhenying Dong Xiangyuan Wan Research Center of Biology and Agriculture Shunde Graduate SchoolUniversity of Science and Technology BeijingBeijing 100024China Zhongzhi International Institute of Agricultural Biosciences Beijing 100192China Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding Beijing International Science and Technology Cooperation Base of Bio-Tech BreedingBeijing Solidwill Sci-Tech Co.Ltd.Beijing 100192China

Plant cell elongation depends on well-defined gene regulations,adequate nutrients,and timely cell wall *** size is positively correlated with the number and viability of pollen grains,while little is known about molecular mechanisms underlying anther cell ***,we found that properly activated cell elongation regulators at transcriptional levels in loss-of-function ZmMs33 mutant(ms33-6038)anthers failed to promote maize anther ***33 deficiency disrupted metabolic homeostasis mainly by inhibiting both photosynthesis in anther endothecium and lipid accumulation in anther ***,ms33-6038 anthers displayed ectopic,premature and excessive secondary cell wall thickening in anther middle layer,which constrained cell elongation structurally and blocked nutrient flows across different anther wall *** metabolic disorder was only found in ms33-6038 mutant rather than several representative male-sterility lines at transcriptional and post-translational ***,the disordered metabolisms and blocked nutrient flows defeated the activated cell elongation regulators,and finally inhibited anther elongation and growth with a unique‘‘idling effect”in ms33-6038 mutant.

关键词： ZmMS33 Anther cell elongation Metabolic homeostasis Secondary cell wall thickening Male sterility Maize

Nanoprobes in biomedical detection

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2016年第3期59卷 255-263页

作者： Yawei zhao Changling Zou Hongkang zhao lina zhao School of Physics Beijing Institute of Technology Beijing 100081 China Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China

Environmental problems caused by the development of nanotechnology have threatened human health. Investigating the biomedical effects of nanomaterials can help to solve these environmental safety issues. In studies on the biomedical effects of nanomaterials, several types of novel nanoscale probes that allow reliable, sensitive, accurate and rapid biomedical detection have emerged. We summarize recent developments in three categories of these nanoprobes, including noble metal nanocluster probes, carbon-based nanostructured probes, and unnatural amino acid-based probes. Besides reviewing the utility of different nanoprobes in cell imaging and protein detection, we also discuss the molecular mechanism of nanoprobe detection. Perspectives of novel nanoprobe design based on molecular details of biomedical detection are presented.

关键词：纳米探针医学检测生物效应环境安全问题生物医学效应非天然氨基酸纳米材料环境问题

A machine learning model for textured X-ray scattering and diffraction image denoising

在线全文

学校读者我要写书评

暂无评论

npj Computational Materials 2023年第1期9卷 1756-1769页

作者： Zhongzheng Zhou Chun Li Xiaoxue Bi Chenglong Zhang Yingke Huang Jian Zhuang Wenqiang Hua Zheng Dong lina zhao Yi Zhang Yuhui Dong Beijing Synchrotron Radiation Facility Institute of High Energy PhysicsChinese Academy of Sciences100049 BeijingChina University of Chinese Academy of Sciences 100049 BeijingChina Spallation Neutron Source Science Center 523803 DongguanGuangdongChina Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of Sciences201204 ShanghaiChina

With the advancements in instrumentations of next-generation synchrotron light sources,methodologies for small-angle X-ray scattering(SAXS)/wide-angle X-ray diffraction(WAXD)experiments have dramatically *** experiments have developed into dynamic and multiscale in situ characterizations,leaving prolonged exposure time as well as radiation-induced damage a serious ***,reduction on exposure time or dose may result in noisier images with a lower signal-to-noise ratio,requiring powerful denoising mechanisms for physical information ***,we tackle the problem from an algorithmic perspective by proposing a small yet effective machine-learning model for experimental SAXS/WAXD image denoising,allowing more redundancy for exposure time or dose *** with classic models developed for natural image scenarios,our model provides a bespoke denoising solution,demonstrating superior performance on highly textured SAXS/WAXD *** model is versatile and can be applied to denoising in other synchrotron imaging experiments when data volume and image complexity is concerned.

关键词： image scattering versatile

微粒脂肪注射对下睑成形术后就医者泪槽宽度和美观度的影响分析

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国美容医学 2024年第2期33卷 1-4页

作者：胡倩文周同葵龚梦童刘晓峰赵莉娜蚌埠市第二人民医院整形外科安徽蚌埠233000 徐州市中心医院整形美容科江苏徐州221000

目的:分析微粒脂肪注射对下睑成形术后就医者泪槽宽度和美观度的影响。方法:选取2022年1月-2022月11月于笔者医院接受治疗的80例泪槽畸形就医者作为研究对象,根据随机数字表法将其分为对照组40例(62眼)与研究组40例(59眼)。对照组就医... 详细信息

目的:分析微粒脂肪注射对下睑成形术后就医者泪槽宽度和美观度的影响。方法:选取2022年1月-2022月11月于笔者医院接受治疗的80例泪槽畸形就医者作为研究对象,根据随机数字表法将其分为对照组40例(62眼)与研究组40例(59眼)。对照组就医者接受标准的下睑成形术,研究组就医者在手术期间收集眶下隔脂肪,下睑成形术后经皮微粒脂肪注射入眶下沟。对比两组就医者的术后情况、美观满意度以及术后并发症发生情况。结果:两组就医者的愈合情况以及随访时间比较差异无统计学意义(P>0.05);研究组就医者瞳孔到眼睑的距离、瞳孔到泪沟的距离以及泪槽宽度较对照组改善明显,且泪槽沟畸形等级量表(Tear trough rating scale,TTRS)评分低于对照组(P0.05)。结论:在下睑成形术中进行微粒脂肪注射可安全有效地改善泪槽畸形,提高颜面部美观度。

关键词：泪槽畸形下睑成形术微粒脂肪注射美观满意度眼睑

维普期刊数据库博看期刊