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Species-level Microbiota of Biting Midges and Ticks from Poyang Lake

Biomedical and Environmental Sciences 2024年第3期37卷 266-277,I0001-I0003页

作者： GONG Jian WANG Fei Fei liU Yang Qing PU Ji DONG ling Zhi ZHANG Si Hui HUANG Zhen Zhou HUANG Yu Yuan li Ya Ben YANG Cai Xin TAO Yuan Meihui zhao li jun JIN Dong liU li Yun YANG Jing LU Shan Department of Epidemiology School of Public HealthShanxi Medical UniversityTaiyuan 030001ShanxiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Jiangxi Provincial Key Laboratory of Animal-Origin and Vector-Borne Diseases Nanchang Center for Disease Control and PreventionNanchang 330038JiangxiChina Research Units of Discovery of Unknown Bacteria and Function Chinese Academy of Medical SciencesBeijing 100730China

Objective The purpose of this study was to investigate the bacterial communities of biting midges and ticks collected from three sites in the Poyang Lake area,namely,Qunlu Practice Base,Peach Blossom Garden,and Huangtong Animal Husbandry,and whether vectors carry any bacterial pathogens that may cause diseases to humans,to provide scientific basis for prospective pathogen discovery and disease prevention and control.Methods Using a metataxonomics approach in concert with full-length 16S rRNA gene sequencing and operational phylogenetic unit(OPU)analysis,we characterized the species-level microbial community structure of two important vector species,biting midges and ticks,including 33 arthropod samples comprising 3,885 individuals,collected around Poyang Lake.Results A total of 662 OPUs were classified in biting midges,including 195 known species and 373 potentially new species,and 618 OPUs were classified in ticks,including 217 known species and 326 potentially new species.Surprisingly,OPUs with potentially pathogenicity were detected in both arthropod vectors,with 66 known species of biting midges reported to carry potential pathogens,including Asaia lannensis and Rickettsia bellii,compared to 50 in ticks,such as Acinetobacter lwoffii and Staphylococcus sciuri.We found that Proteobacteria was the most dominant group in both midges and ticks.Furthermore,the outcomes demonstrated that the microbiota of midges and ticks tend to be governed by a few highly abundant bacteria.Pantoea sp7 was predominant in biting midges,while Coxiella sp1 was enriched in ticks.Meanwhile,Coxiella spp.,which may be essential for the survival of Haemaphysalis longicornis Neumann,were detected in all tick samples.The identification of dominant species and pathogens of biting midges and ticks in this study serves to broaden our knowledge associated to microbes of arthropod vectors.Conclusion Biting midges and ticks carry large numbers of known and potentially novel bacteria,and carry a wide range of potentially pathogenic bacteria,which m

关键词： Biting midge Tick Microbiota Pantoea Coxiella

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Extracorporeal membrane oxygenation states basilar artery thrombectomy and left posterior cerebral artery stent thrombectomy:A case report

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World Journal of Clinical Cases 2024年第18期12卷 3589-3595页

作者： li-Shan Ding Hao liang Ming Zheng Meng Shen zhao-jun li Rui-Peng Song Qing-liang Chen Department of Radioactive Intervention Henan University of Traditional Chinese MedicineZhengzhou 450099Henan ProvinceChina Department of Radioactive Intervention Henan No.3 Provincial People’s HospitalZhengzhou 450006Henan ProvinceChina Department of Endocrinology Henan No.3 Provincial People’s HospitalZhengzhou 450000Henan ProvinceChina

BACKGROUND Extracorporeal membrane oxygenation(ECMO)is a new type of extracorporeal respiratory and circulatory assistance device.It can drain venous blood out of the body and inject it into veins or arteries after being oxygenated by an oxygenator(membrane lung)to replace lung and heart functions in a short time.ECMO can provide tissue blood perfusion and gas exchange almost equivalent to cardiac output and extend the effective treatment time window for patients with acute circulatory failure to restore cardiopulmonary function.CASE SUMMARY We report a case of an 81-year-old woman who underwent whole cerebral angiography,basilar artery thrombectomy and stent thrombectomy in the posterior artery of the left brain after implantation of ECMO.The patient was admitted to the hospital due to myocardial infarction.Considering that the cause of the patient’s disturbance of consciousness was unknown and cerebrovascular accident could not be ruled out after the implantation of ECMO,the department of Radioactive Intervention performed cerebral angiography.And the result of the angiography indicated vascular occlusion.After the basilar artery thrombectomy and stent thrombectomy in the posterior artery of the left brain,the patency of the occlusive vessel was achieved.CONCLUSION Although the patient eventually died of circulatory failure,the result of this case verifies the feasibility of cerebral angiography and thrombectomy in patients with implanted ECMO in the intubated state.

关键词： Extracorporeal membrane oxygenation Blood circulation failure Nerve intervention Stent thrombectomy Case report

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Petrochemical wastewater treatment with a pilot-scale bioaugmented biological treatment system

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第11期8卷 1831-1838页

作者： zhao li-jun MA Fang GUO Jing-bo zhao Qing-liang School of Chemical Engineering China University of Petroleum Beijing 102249 China School of Municipal and Environmental Engineering Harbin Institute of Technology Harbin 150090 China

In solving the deterioration of biological treatment system treating petrochemical wastewater under low temperatures, bioaugmentation technology was adopted by delivering engineering bacteria into a pilot-scale two-stage anoxic-oxic (A/O) process based on previous lab-scale study. Experimental results showed that when the concentrations of COD and NH4+-N of the influent were 370~910 mg/L and 10~70 mg/L, the corresponding average concentrations of those of effluent were about 80 mg/L and 8 mg/L respectively, which was better than the Level I criteria of the Integrated Wastewater Discharge Standard (GB8978-1996). According to GC-MS analysis of the effluents from both the wastewater treatment plant (WWTP) and the pilot system, there were 68 kinds of persistent organic pollutants in the WWTP effluent, while there were only 32 in that of the pilot system. In addition, the amount of the organics in the effluent of the pilot system reduced by almost 50% compared to that of the WWTP. As a whole, after bioaugmentation, the organic removal efficiency of the wastewater treatment system obviously in- creased.

关键词：生物自检法石化产品水污染环境保护

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Histone deacetylase inhibitors inducing human cervical cancer cell apoptosis by decreasin~ DNA-methyltransferase 3B

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Chinese Medical Journal 2012年第18期125卷 3273-3278页

作者： liU Ning zhao li-jun li Xiao-ping WANG Jian-liu CHAI Guo-lin WEI li-hui Department of Obstetrics and Gynecology Peking UniversityPeople's Hospital China Beijing 100044 Department of Hematology China-Japan Friendship Hospital China Beijing 100029

Background Histone deacetylase （HDAC） inhibitors are a group of small chemical molecules that inhibit histone deacetylase. At cell level, HDAC inhibitors have multiple biological effects such as cell cycle arrest, apoptosis, cell differentiation and auotophagy. At molecular level, HDAC inhibitors cause histone and nonhistone acetylation and induce gene expression. HDAC inhibitors are widely used in cancer therapy because of its function of inducing apoptosis. However, the mechanisms of apoptosis effect are not fully understood. TSA is a classical HDAC inhibitor and widely used in epigenetic and anti-cancer research. In this study, we selected Trichostatin A （TSA） to investigate the mechanisms of HDAC inhibitors apoptotic effect on cancer cells. Methods Cervical cancer cell lines such as Hela, Caski and normal human keratinocyte line HaCaT were treated with various concentrations of TSA. Crystal violent assay and 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay were performed to determine cell number. PARP cleavage and FITC-AnexinV were performed to determine apoptosis. DNA-methyltransferase （DNMT）I, DNMT3A and DNMT3B were determined by regular PCR, qPCR and Western Blotting. Small interfering RNA （SiRNAi） was used to knock down DNMT3B. Results HDAC inhibitors only induce cervical cancer cell apoptosis. At 1 Iumol/L of TSA, 86% of Hela cell and 76% of Caski went apoptosis. For normal cells, HDAC inhibitors have no cytotoxic effect at therapeutic dosage, （90.0＋8.4）% of normal cell survive after treated with 1 IJmol/L of TSA. We compared 1 pmol/L group with untreated control with t-test. There was no significance between 1 pmol/L group and untreated control for normal cell （P 〉0.05）. HDAC inhibitors decreased DNMT3B in cancer cell but not in normal cell. Manually knock-down of DNMT3B induced Hela and Caski cell apoptosis. More than 99% of Hela and Caski cell went apoptosis after deprived of DNMT3B. Conclusions DNMT3B was essential to cervical cancer cell survival. Down-regulated DNMT3B by HDA

关键词： histone deaeetylase inhibitors human cervical cancer apoptosis DNA-methyltransferase 3B Trichostatin A DNA methyl transferase

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Dexamethasone protects airway epithelial cell line NCI-H292 against lipopolysaccharide induced endoplasmic reticulum stress and apoptosis

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Chinese Medical Journal 2011年第1期124卷 38-44页

作者： SHANG Yan WANG Fang BAI Chong HUANG Yi zhao li-jun YAO Xiao-peng li Qiang SUN Shu-han Department of Respiratory Medicine Changhai Hospital Second Military Medical University Shanghai 200433 China Department of Medical Genetics Second Military Medical University Shanghai 200433 China

Background Endoplasmic reticulum （ER） stress and ER stress-mediated apoptosis were reported to be involved in the pathogenesis of several diseases. In a recent study, it was reported that the ER stress pathway was activated in the lungs of lipopolysaccharide （LPS）-treated mice. It was also found that the C/EBP homologous protein （CHOP）, an apoptosis-related molecule, played a key role in LPS-induced lung damage. The aim of this study was to verify whether LPS could activate the ER stress response in airway epithelial cells and which molecule was involved in the pathway. This study was also aimed at finding new reagents to protect the airway epithelial cells during LPS injury. Methods ER stress markers were observed in LPS-incubated NCI-H292 cells. SiRNA-MUC5AC was transfected into NCI-H292 cells. The effects of dexamethasone and erythromycin were observed in LPS-induced NCI-H292 cells. Results LPS incubation increased the expression of ER stress markers at the protein and mRNA levels. The knockout of MUC5AC in cells attenuated the increase in ER stress markers after incubation with LPS. Dexamethasone and erythromycin decreased caspase-3 activity in LPS-induced NCI-H292 cells. Conclusions LPS may activate ER stress through the overexpression of MUC5AC. Dexamethasone may protect human airway epithelial cells against ER stress-related apoptosis by attenuating the overload of MUC5AC.

关键词： airway epithelial cells endoplasmic reticulum stress apoptosis lipopolysaccharide dexamethasone

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光照和水分对四季米仔兰幼苗生长的影响

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应用生态学报 2024年第2期35卷 439-446页

作者：甘菲菲招礼军霍灿灿王晟广西大学林学院广西森林生态与保育重点实验室南宁530004

四季米仔兰栽培历史悠久,是重要的造景树种。以1年生四季米仔兰幼苗为研究对象,设置3个光照处理(透光度为70%、50%、30%)和3个水分处理(田间持水量为70%、50%、30%),以透光度90%、田间持水量90%为对照,分析不同环境条件下四季米仔兰幼... 详细信息

四季米仔兰栽培历史悠久,是重要的造景树种。以1年生四季米仔兰幼苗为研究对象,设置3个光照处理(透光度为70%、50%、30%)和3个水分处理(田间持水量为70%、50%、30%),以透光度90%、田间持水量90%为对照,分析不同环境条件下四季米仔兰幼苗的生长状况和光合生理指标。结果表明:干旱胁迫和遮光抑制四季米仔兰幼苗的生长,且干旱胁迫的影响大于遮光。随着土壤水分的减少,株高增量、地径增量、净光合速率、蒸腾速率、气孔导度和叶绿素含量均显著降低,最大降幅分别为71.4%、81.2%、93.2%、71.5%、70.6%、30.4%;适当遮光(透光度50%)可缓解严重水分亏缺(田间持水量30%)对四季米仔兰幼苗生长的不利影响。透光度70%和田间持水量70%条件下株高增量、叶面积、净光合速率、蒸腾速率和气孔导度最大。最大荧光、可变荧光、PSⅡ潜在活性、PSⅡ最大光能转化效率随着水分减少呈先上升后下降趋势。四季米仔兰幼苗能够通过调整生长、提高叶绿素含量和调节光合系统来维持光合作用以适应胁迫环境。透光度70%和田间持水量70%有利于四季米仔兰幼苗光合作用的发挥,对其生长有积极影响。

关键词：光照水分生长特性光合作用四季米仔兰

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新型纤维增强聚合物钢筋混凝土桥面板性能研究

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鄂州大学学报 2024年第1期31卷 109-112页

作者：赵利军中铁十八局集团有限公司投资公司天津300222

桥梁的钢筋腐蚀会严重影响桥梁的使用寿命,增加维护成本。该文介绍了纤维增强聚合物钢筋在混凝土桥梁的创新应用,包括桥梁的施工细节、现场测试和有限元分析。混凝土桥面板使用不同类型的FRP钢筋以及普通钢筋。在桥梁的关键位置安装了... 详细信息

桥梁的钢筋腐蚀会严重影响桥梁的使用寿命,增加维护成本。该文介绍了纤维增强聚合物钢筋在混凝土桥梁的创新应用,包括桥梁的施工细节、现场测试和有限元分析。混凝土桥面板使用不同类型的FRP钢筋以及普通钢筋。在桥梁的关键位置安装了光纤传感器用于收集内部温度和应变等数据,这些传感器用于监测施工期间至施工完成后桥梁的性能变化。使用校准的卡车荷载测试桥梁的使用性能。同时,进行了有限元分析,并根据现场载荷试验的结果进行了验证。然后,利用有限元分析进行参数研究,以研究FRP加固类型和比例等重要参数对桥梁的适用性和耐久性的影响。研究发现,钢筋和混凝土在实际使用条件下量测的挠度、裂缝、应变等结果与有限元分析的结果基本相同。

关键词：桥梁桥面板 FRP钢筋现场测试有限元分析

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Bronchoscopy in some tertiary grade A hospitals in China： two years＇ development

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Chinese Medical Journal 2012年第12期125卷 2115-2119页

作者： NIE Xiao-meng CAI Gang SHEN Xian YAO Xiao-peng zhao li-jun HUANG Yi HAN Yi-ping BAI Chong li Qiang Department of Respiratory Diseases Changhai Hospital SecondMilitary Medical University Shanghai 200433 China Department of Clinical Laboratory Ruijin Hospital Shanghai JiaoTong University Shanghai 200025 China

Background Although bronchoscopy has been widely performed in China, little has been known about its current state and development. In order to investigate the clinical application of bronchoscopy and make instructions for future education and development, the Chinese Society of Respiratory Diseases conducted postal surveys in both 2008 and 2010 in China. Method Questionnaires were sent to 40 tertiary grade A hospitals in 2008 and 58 tertiary grade A hospitals in 2010 to investigate bronchoscopies performed in 2007 and 2009 respectively. Results Thirty （75%） hospitals returned the completed questionnaires in 2008 and forty-one （71%） hospitals in 2010. All the respondents possessed flexible bronchoscopes. Fifty percent of the respondents had less than five in 2007, while more than 50% of the respondents had 5-9 bronchoscopes in 2009. All the respondents performed a radiograph or CT scan before bronchoscopy. Percentage of general anesthesia and no pre-medication before bronchoscopy increased, while atropine usage decreased in 2009 compared to 2007. During bronchoscopy, pulse oximetry was the most widely used monitoring method. Most respondents used the nasal route to perform routine bronchoscopy. After the procedure, they used sinks to wash and glutaraldehyde to disinfect the bronchoscopes. The total number of flexible bronchoscopies performed during 2007 was 37 874 and the average was 1262. Whereas in 2009, the total number was 60 178 and the average was 1468. Diagnostic bronchoscopy was more widely used than therapeutic bronchoscopy. The mortality rate was 0.076%0 in 2007 and 0.032%0 in 2009. Conclusions The two surveys, to some extent, reflected the current status and development of bronchoscopy in China. The results are worthy of future education and developing of new guidelines. Regular surveys and monitoring of bronchoscopies across China are needed.

关键词： bronchoscopy survey clinical research diagnostic and therapeutic bronchology

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对强震地形变监测预报方法的思考

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地震 2024年第1期44卷 64-77页

作者：薄万举张立成苏国营徐东卓赵立军中国地震局第一监测中心天津300180

简要回顾了中国地形变用于强震监测预报领域的发展过程及现状。对几次强震前得到的比较突出的地形变异常进行了分析,收集了大面积形变异常与强震中长期预测、地震前兆分布存在时间有序和空间配套,强震前发现巨幅形变异常,InSAR给出强震... 详细信息

简要回顾了中国地形变用于强震监测预报领域的发展过程及现状。对几次强震前得到的比较突出的地形变异常进行了分析,收集了大面积形变异常与强震中长期预测、地震前兆分布存在时间有序和空间配套,强震前发现巨幅形变异常,InSAR给出强震前地面垂向形变,强震前地倾斜异常,慢地震和预滑移等方面的研究成果。综合分析认为,强震前震中区附近存在与孕震体尺度相当的巨幅快速地面异常隆升。及时有效地捕捉强震前巨幅快速地面异常隆升的时间有序和空间配套的异常信息,可望针对人口稠密区域未来发生的强震给出具有减灾实效的预测预报意见,值得尝试。基于本研究提出了针对人口稠密的地震重点监视防御区开展高密度、大量程、低精度地倾斜观测的设想,并初步给出了观测方案、基本原理和数据计算处理方法。

关键词：强震预报地形变监测巨幅形变地倾斜地面隆起

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模袋护坡工程GPR检测机理和应用研究

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中国农村水利水电 2024年第7期 170-176,182页

作者：袁金峰赵丽君崔洪礼张丽梅鞠政李健吉林省水利科学研究院吉林长春130022 吉林大学材料科学与工程学院汽车材料教育部重点实验室吉林长春130022

GPR技术近年来在许多领域得到了广泛应用,但公路工程在道路脱空探测时对于充水型脱空无法检测,且从未在模袋护坡工程检测中应用。鉴于此,通过室内模型试验,研究了模袋含水率对波速和相对介电常数的影响,并分析了电磁波波长在模袋中的变... 详细信息

GPR技术近年来在许多领域得到了广泛应用,但公路工程在道路脱空探测时对于充水型脱空无法检测,且从未在模袋护坡工程检测中应用。鉴于此,通过室内模型试验,研究了模袋含水率对波速和相对介电常数的影响,并分析了电磁波波长在模袋中的变化情况;通过水池模型试验,在不同电导率的水中对电磁波波速、波长、衰减常数和趋肤深度进行了研究分析。结果表明:模袋护坡工程探测时,应尽量选择高频天线,以提高钢筋分辨率,且重点考虑模袋含水率的影响,选取准确的相对介电常数,准确计算钢筋位置。充水型脱空探测时,应重点考虑水中电磁波的衰减,高频段衰减程度取决于水的电导率。当水的电导率不大于0.1 s/m时,高频段天线均可探测到脱空层底部;当水的电导率大于0.1 s/m和小于1 s/m时,高频段天线均识别困难;当电导率达到1 s/m时,高频段的所有频率均无法识别。

关键词： GPR技术模袋脱空检测相对介电常数电导率充水型脱空

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