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Hypoxia activates the hypoxia-inducible factor-1α/vascular endothelial growth factor pathway in a prostatic stromal cell line:A mechanism for the pathogenesis of benign prostatic hyperplasia

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Current Urology 2024年第3期18卷 185-193页

作者： Tao Zhang Changlin Mao Yao Chang jiaju Lyu Delong zhao Sentai Ding Department of Urology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinanChina Department of Urology Mindong Hospital Affiliated to Fujian Medical UniversityFuanChina

Background The development of benign prostatic hyperplasia(BPH)is closely related to hypoxia in the prostatic stroma,and the hypoxia-inducible factor-1α/vascular endothelial growth factor(HIF-1α/VEGF)pathway has been shown to significantly activate in response to *** underlying mechanism for activation of this pathway in the pathogenesis of BPH remains *** and methods We constructed HIF-1αoverexpression and knockdown BPH stromal(WPMY-1)and epithelial(BPH-1)cell lines,which were cultured under different oxygen conditions(hypoxia,normoxia,and hypoxia+HIF-1αinhibitor).Quantitative real-time polymerase chain reaction(qPCR)and Western blotting were applied to detect the expression of the HIF-1α/VEGF *** proliferation and apoptosis were analyzed by Cell Counting Kit-8 and flow *** used the miRWalk 2.0 database and Western blotting to predict the potential miRNA that selectively targets the HIF-1α/VEGF pathway,and verified the prediction by qPCR and dual-luciferase *** In a BPH stromal cell line(WPMY-1),the expression of VEGF was in accordance with HIF-1αlevels,elevated in the overexpression cells and decreased in the knockdown ***-induced HIF-1αoverexpression,which could be reversed by a HIF-1α***,the HIF-1αinhibitor significantly depressed cellular proliferation and promoted apoptosis in hypoxic conditions,assessed by Cell Counting Kit-8 and flow ***,in the BPH epithelial cell line(BPH-1),the expression level of HIF-1αdid not influence the expression of ***,a potential miRNA,miR-17-5p,regulating the HIF-1α/VEGF pathway was predicted from the miRWalk 2.0 database and Western blotting,and verified by qPCR and dual-luciferase *** In hypoxia,activation of the HIF-1α/VEGF pathway plays a crucial role in regulating cell proliferation in a BPH stromal cell *** by miR-17-5p may be the potential mechanism for the activation of this *** of thi

关键词： Benign prostatic hyperplasia Hypoxia-inducible factor-1α Vascular endothelial growth factor Pathogenesis miR-17-5p

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百香果茎尖组织培养体系的建立

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热带农业科学 2020年第11期40卷 75-80页

作者：赵家桔高玲李莉萍张如莲高锦合刘迪发徐丽应东山中国热带农业科学院热带作物品种资源研究所海南海口571101 海南省南繁生物安全与分子育种重点实验室海南海口571101

以黄金百香果茎尖为试验材料,进行茎尖离体芽的诱导和培养,研究茎尖外植体消毒最佳方式与时间,初代、继代增殖及生根培养过程中适宜的激素种类及浓度。结果表明:最佳的消毒方式为75%的酒精溶液消毒30 s后再用0.1%HgCl2消毒10 min;最适... 详细信息

以黄金百香果茎尖为试验材料,进行茎尖离体芽的诱导和培养,研究茎尖外植体消毒最佳方式与时间,初代、继代增殖及生根培养过程中适宜的激素种类及浓度。结果表明:最佳的消毒方式为75%的酒精溶液消毒30 s后再用0.1%HgCl2消毒10 min;最适初代培养基为MS+6-BA 2.0 mg/L+GA30.5 mg/L+NAA 0.1 mg/L,有利于丛生芽的诱导;最适继代增殖培养基为MS+6-BA 1.5 mg/L+NAA 0.1 mg/L,适宜芽的增殖且利于壮苗;最适生根培养基为1/2 MS+NAA 0.1 mg/L+IBA 0.1 mg/L,形成的苗健壮,生根率高达95%。

关键词：百香果茎尖培养组培体系

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Crosstalk between PC 12 cells and endothelial cells in an artificial neurovascular niche constructed by a dual-functionalized selfassembling peptide nanofiber hydrogel

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Nano Research 2022年第2期15卷 1433-1445页

作者： Zhe Zhang Yi Chai He zhao Shuhui Yang Wei Liu Zihui Yang Weilong Ye Chenlong Wang Xiaohan Gao Xiangdong Kong Xiaodan Sun Lingyun zhao Tuoyu Chen Yuqi Zhang jiaju Lu Xiumei Wang State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Department of Neurosurgery Yuquan HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 100084China Institute of Smart Biomedical Materials School of Materials Science and EngineeringZhejiang Sci-Tech UniversityHangzhou 310018China Department of orthopedics Dongzhimen hospitalBeijing University of Chinese MedicineBeijing 100700China Department of Neurosurgery The First Hospital of Hebei Medical UniversityShijiazhuang 050000China Department of Obstetrics and Gynecology Beijing Tsinghua Changgung HospitalBeijing 102218China Department of Chemistry Tsinghua UniversityBeijing 100084China

The coordination between neurogenesis and angiogenesis plays an important role in nerve tissue development and ***,using bioactive materials to drive neurogenic and angiogenic responses has gained increasing *** the neurovascular link between regulatory cues offers valuable insight into the mechanisms underlying nerve regeneration and the design of new bioactive *** this study,we utilized a dual-functionalized peptide nanofiber hydrogel presenting the brain-derived neurotrophic factor and vascular endothelial growth factor mimetic peptides RGIDKRHWNSQ(RGI)and KLTWQELYQLKYKGI(KLT)to construct an artificial neurovascular *** dual-functionalized peptide nanofiber hydrogel enhanced the neurite outgrowth of pheochromocytoma(PC12)cells and tube-like structures formation of human umbilical vein endothelial cells(HUVECs)in vitro,and promoted rapid lesion infiltration of neural and vascular cells in a rat brain injury *** indirect co-culture models,we found that the dual-functionalized peptide hydrogel effectively mediated neurovascular crosstalk by regulating secretion of paracrine factors from PC12 cells and *** the two cells types were directly co-cultured on the dua卜functionalized peptide hydrogel,the efficiency of cell-cell communication was enhanced,which further accelerated the differentiation and maturation of PC12 cells with an increased number of pseudopodia and spread morphology,and HUVECs tube-like structure *** summary,the dual-functionalized peptide nanofiber hydrogel successfully formed an artificial neurovascular niche to directly regulate the behaviors of neural and vascular cells and promote their neurovascular crosstalk through paracrine signaling and direct cell-cell contact.

关键词： neurovascular microenvironment self-assembling peptide co-culture nanofiber hydrogel brain injury

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A neurotrophic peptide-functionalized self-assembling peptide nanofiber hydrogel enhances rat sciatic nerve regeneration

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Nano Research 2018年第9期11卷 4599-4613页

作者： jiaju Lu Xun Sun Heyong Yin Xuezhen Shen Shuhui Yang Yu Wang Wenli Jiang Yue Sun Lingyun zhao Xiaodan Sun Shibi Lu Antonios G. Mikos Jiang Peng Xiumei Wang State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and Engineering Tsinghua University Beijing 100084 China Institute of Orthopedics Chinese PLA General Hospital Beijing 100853 China School of Medicine Nankai University Tianjin 300071 China Experimental Surgery and Regenerative Medicine Department of Surgery Ludwig-Maximilians-University Munich 80336 Germany Department of Ultrasound Beijing Hospital Beijing 100730 China CenterofBiomedicalAnalysis Tsinghua University Beijing 100084 China Department of Bioengineering Bioscience Research Collaborative Rice University Texas 77030 USA Co-innovation Center of Neuroregeneration Nantong University Nantong 226007 China

Nerve guidance conduit （NGC） is a potential alternative to autologous nerve for peripheral nerve regeneration. A promising therapeutic strategy is to modify the nerve guidance conduit intraluminal microenvironment using physical and/or chemical guidance cues. In this study, a neurotrophic peptide-functionalized self-assembling peptide nanofiber hydrogel that could promote PC12 cell adhesion, proliferation, and neuronal differentiation in vitro was prefilled in the lumen of a hollow chitosan tube （hCST） to accelerate axonal regeneration in a rat sciatic nerve defect model. The functionalized self-assembling peptide was developed by introducing a neurotrophic peptide （RGI, RGIDKRHWNSQ） derived from brain-derived neurotrophic factor （BDNF） to the C-terminus of the self-assembling peptide RADA16-I （Ac-（RADA）4-CONH2）. Morphological, histological, electrophysiological, and functional analyses demonstrated that the RGI-functionalized, self-assembling, peptide nanofiber hydrogel RAD/RGI could produce a neurotrophic microenvironment that markedly improved axonal regeneration with enhanced re-myelination and motor functional recovery.

关键词： peripheral nerve regeneration,self-assembling peptide,hydrogel,neurotrophic peptide,intraluminal microenvironment

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Construction and evaluation of a novel prognostic risk model of aging-related genes in bladder cancer

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Current Urology 2023年第4期17卷 236-245页

作者： Delin Wang Hao Ning Haihu Wu Yufeng Song Yaru Chu Feifan Liu Zhenlin zhao Fei Wu jiaju Lyu Department of Urology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan250021China Shandong First Medical University and Shandong Academy of Medical Sciences JinanChina Department of Urology Shandong Provincial Hospital Affiliated to Shandong UniversityJinanChina Department of Urology The Second Hospital of Shandong UniversityJinanChina

Background:Bladder cancer(BLCA)is the most common malignancy of the urinary ***-invasive bladder cancer(MIBC),which constitutes approximately 25%of all BLCA cases,is characterized by frequent recurrence and early onset of *** cancer most commonly occurs in elderly patients and is significantly associated with ***,the prognostic value of age-related genes in BLCA,especially in MIBC,remains *** and methods:Training and testing sets were obtained from The Cancer Genome Atlas BLCA *** expressed genes between BLCA and normal samples intersected with human aging-related *** Cox regression and least absolute shrinkage and selection operator regression analyses were used to identify prognostic aging-related signatures,followed by the construction of a risk score model and ***-Meier and receiver operating characteristic analyses were conducted to assess the predictive *** independent BLCA cohort of 165 samples was included for external *** CIBERSORT algorithm was used to explore the characteristics of the immune ***:Seven genes(IGF1,NGF,GCLM,PYCR1,EFEMP1,APOC3,and IFNB1)were identified by Cox and least absolute shrinkage and selection operator *** combining the gene signature with the clinical parameters of patients with BLCA,a risk-prognosis model and nomogram were constructed and validated with the testing *** cancer cases with high 7-gene signature scores(high-risk group)and low scores(low-risk group)showed distinct ***,7 types of immune cells were significantly altered between the low-and high-risk ***:Collectively,our data provide a 7-gene signature that serves as a potential biomarker for BLCA,especially ***,this 7-gene signature highlights the role of the tumor immune microenvironment in prognosis and thus might be related to the response to anti-programmed cell death protein 1-b

关键词： Bladder cancer Prognosis Aging-related genes Biomarker Immune microenvironment Overall survival

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Aligned fibrin/functionalized self-assembling peptide interpenetrating nanofiber hydrogel presenting multi-cues promotes peripheral nerve functional recovery

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Bioactive Materials 2022年第2期7卷 529-544页

作者： Shuhui Yang Jinjin Zhu Changfeng Lu Yi Chai Zheng Cao jiaju Lu Zhe Zhang He zhao Yin-Yuan Huang Shenglian Yao Xiangdong Kong Peixun Zhang Xiumei Wang State Key Laboratory of New Ceramics and Fine Processing Key Laboratory of Advanced Materials of Ministry of EducationSchool of Materials Science and EngineeringTsinghua UniversityBeijing100084PR China Key Laboratory of Trauma and Neural Regeneration Peking UniversityMinistry of EducationDepartment of Trauma and OrthopedicsPeking University People’s HospitalBeijing100044PR China Department of Orthopaedic Surgery Sir Run Run Shaw HospitalZhejiang University School of Medicine&Key Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of ZhejiangHangzhou310016PR China School of Clinical Medicine Tsinghua UniversityBeijing100084PR China School of Materials Science and Engineering University of Science and Technology BeijingBeijing100083PR China School of Materials Science and Engineering Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue EngineeringZhejiang Sci-Tech UniversityHangzhou310018PR China

Nerve guidance conduits with hollow lumen fail to regenerate critical-sized peripheral nerve defects(15 mm in rats and 25 mm in humans),which can be improved by a beneficial intraluminal ***,individual cues provided by intraluminal filling materials are inadequate to eliminate the functional gap between regenerated nerves and normal ***,an aligned fibrin/functionalized self-assembling peptide(AFG/fSAP)interpenetrating nanofiber hydrogel that exerting synergistic topographical and biochemical cues for peripheral nerve regeneration is constructed via electrospinning and molecular *** hydrogel possesses an aligned structure,high water content,appropriate mechanical properties and suitable biodegradation capabilities for nerve repair,which enhances the alignment and neurotrophin secretion of primary Schwann cells(SCs)in vitro,and successfully bridges a 15-mm sciatic nerve gap in rats in *** rats transplanted with the AFG/fSAP hydrogel exhibit satisfactory morphological and functional recovery in myelinated nerve fibers and innervated *** motor function recovery facilitated by the AFG/fSAP hydrogel is comparable with that of ***,the AFG/fSAP hydrogel upregulates the regeneration-associated gene expression and activates the PI3K/Akt and MAPK signaling pathways in the regenerated ***,the AFG/fSAP hydrogel represents a promising approach for peripheral nerve repair through an integration of structural guidance and biochemical stimulation.

关键词： Peripheral nerve regeneration Alignment Nanofibers Fibrin Self-assembling peptides

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基于景观评价的河道景观规划方法研究——以昆明市盘龙江为例

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北京大学学报（自然科学版） 2019年第1期55卷 189-196页

作者：刘佳驹王宇泓赵龙郭怀成北京大学环境科学与工程学院北京100871 北京林业大学园林学院北京1000871 河北软件职业技术学院保定071000

以河道景观评价为目标层,以生态性、社会性和艺术性指标作准则层,以水质、物种多样性、水体透明度、防洪效应、水景利用率、景观可达性、色彩美感度、形态美感度以及地域文化性作为指标层,构建城市河道景观评价指标体系。利用层次分析法... 详细信息

以河道景观评价为目标层,以生态性、社会性和艺术性指标作准则层,以水质、物种多样性、水体透明度、防洪效应、水景利用率、景观可达性、色彩美感度、形态美感度以及地域文化性作为指标层,构建城市河道景观评价指标体系。利用层次分析法(AHP)确定各指标层的权重,利用距离指数法构建评价模型。以昆明市盘龙江为例,利用该模型对盘龙江上中下游景观环境进行评价。将定量评价与定性评价相结合,对城市水系景观环境规划设计提供指导,构建水景观规划新模式,以期为实现科学景观规划设计提供参考。

关键词：层次分析法河道景观规划盘龙江景观评价

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多源Tomosynthesis迭代算法在动态模型中的应用

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中国医疗器械杂志 2011年第4期35卷 250-252,262页

作者：彭家驹赵俊上海交通大学生物医学工程系医学图像处理实验室上海市200240

提出了一种新的tomosynthesis扫描方法,并将该方法应用到动态重建场合,用于抑制运动在成像中产生的伪迹。新的方法采用多源(双源与四源)环形扫描方式和迭代重建算法对两种动态数字模型进行仿真重建。通过比较单源重建和多源重建的结果,... 详细信息

提出了一种新的tomosynthesis扫描方法,并将该方法应用到动态重建场合,用于抑制运动在成像中产生的伪迹。新的方法采用多源(双源与四源)环形扫描方式和迭代重建算法对两种动态数字模型进行仿真重建。通过比较单源重建和多源重建的结果,评价多源tomosynthesis在动态重建方面的性能。结果表明多源tomosynthesis的扫描方式能够有效抑制动态重建中的伪迹,改善重建质量。本文的结果证实了多源tomosynthesis在动态重建中的有效性,对于进一步改善心脏和呼吸运动成像质量具有积极的实际意义。

关键词： tomosynthesis 多源动态模型

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Observation of maximal intrinsic chirality empowered by dual quasi-bound states in the continuum in a planar metasurface

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Photonics Research 2024年第2期12卷 244-252页

作者： XIN QI jiaju WU FENG WU SONG zhao CHAO WU YUEYANG MIN MINA REN YUFEI WANG HAITAO JIANG YUNHUI LI ZHIWEI GUO YAPING YANG WANHUA ZHENG HONG CHEN YONG SUN MOE Key Laboratory of Advanced Micro-Structured Materials School of Physics Science and EngineeringTongji UniversityShanghai 200092China School of Optoelectronic Engineering Guangdong Polytechnic Normal UniversityGuangzhou 510665China l3 Shanghai Key Laboratory of Special Artificial Microstructure Materials and Technology School of Physics Science and EngineeringTongji UniversityShanghai 200092China Department of Electrical Engineering Tongji UniversityShanghai 201804China State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China

Metasurfaces with spin-selective transmission play an increasingly critical role in realizing optical chiral responses,especially for strong intrinsic chirality,which is limited to complex three-dimensional *** this paper,we propose a planar metasurface capable of generating maximal intrinsic chirality and achieving dual-band spinselective transmission utilizing dual quasi-bound states in the continuum(quasi-BICs)caused by the structural symmetry ***,the value of circular dichroism(CD)and the transmittance of two kinds of circular polarization states can be arbitrarily controlled by tuning the asymmetry *** CD approaching unity with the maximum transmittance up to 0.95 is experimentally achieved in the dual ***,assisted by chiral BICs,the application in polarization multiplexed near-field image display is also *** work provides a new avenue to flexibly control intrinsic chirality in planar structure and offers an alternative strategy to develop chiral sensing,multiband spin-selective transmission,and high-performance circularly polarized wave *** basic principle and design method of our experiments in the microwave regime can be extended to other bands,such as the terahertz and infrared wavelengths.

关键词： planar intrinsic quasi

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烧结电镀强浸润管壁结构强化R245fa流动沸腾特性的实验研究

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工程热物理学报 2023年第5期44卷 1366-1375页

作者：曹泷王光辉郭家驹赵露星杨辉郑州轻工业大学能源与动力工程学院郑州450001

实验研究了有机工质R245fa在内径为10 mm的不锈钢光管和烧结电镀多尺度镀层强化管内的流动沸腾传热特性。实验工况为:饱和压力0.6 MPa,质量流速189.3~708.14 kg·s^(-1)·m^(-2),热流密度4.94~44.74 k W·m^(-2),干度0.01~0.9。实验结... 详细信息

实验研究了有机工质R245fa在内径为10 mm的不锈钢光管和烧结电镀多尺度镀层强化管内的流动沸腾传热特性。实验工况为:饱和压力0.6 MPa,质量流速189.3~708.14 kg·s^(-1)·m^(-2),热流密度4.94~44.74 k W·m^(-2),干度0.01~0.9。实验结果表明:实验条件下观测到分层流与环状流两种流型,发现管内多尺度强浸润镀层能够促进环状流的转变提前。与光管相比,烧结电镀多尺度镀层强化管具有明显的强化沸腾换热的效果,其传热强化因子平均为1.87,最大强化效果为3.15。随着热流密度以及干度的增大,传热强化因子先增加后降低。流型可视化对比发现,强化管壁面的强润湿性促进上壁面液相吸附以及快速再浸润,对流动沸腾换热具有积极作用。

关键词：流动沸腾 R245fa 表面浸润性强化传热烧结电镀

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