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检索条件"作者=ZHAO Hongxin1,2,3, XIE Baoen2 & CHEN Sanfeng1,2 1. State Key Laboratory for Agrobiotechnology and Biological School, china agricultural university, Beijing 100094, china"
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Cloning and sequencing of nifBHDKENX genes of Paenibacillus massiliensis T7 and its nif promoter analysis
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Science china(Life Sciences) 2006年 第2期49卷 115-122页
作者: zhao hongxin1,2,3, xie baoen2 & chen sanfeng1,2 1. state key laboratory for agrobiotechnology and biological school, china agricultural university, beijing 100094, china 2. key laboratory for Agro-Microbial Resource and Application of Agriculture Ministry, china agricultural university, beijing 100094, china 3. Chemical and biological school, ShenYang Normal university, Shenyang 110034, china 1. state {1. laboratory for agrobiotechnology and biological school China Agricultural University Beijing 100094 China 2. key {1. for Agro-Microbial Resource and Application of Agriculture Ministry China Agricultural University Beijing 100094 China 3. Chemical and biological {1. ShenYang Normal University Shenyang 110034 China
A 324 bp of nifH fragment was PCR amplified from Paenibacillus massiliensis T7 using the universal degenerate primers. The PCR-amplified nifH fragment was labeled with DIG; then used as a probe in Southern blot analys... 详细信息
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