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TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken

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Journal of Integrative Agriculture 2023年第3期22卷 853-863页

作者： CUI Huan-xian LUO Na GUO Li-ping LIU Lu XING Si-yuan zhao gui-ping WEN Jie State Key Laboratory of Animal Nutrition/Institute of Animal Sciences Chinese Academy of Agricultural SciencesBeijing 100193P.R.China

The interaction between myocytes and intramuscular adipocytes is a hot scientific topic. Using a co-culture system, this study aims to investigate the regulation of intramuscular fat deposition in chicken muscle tissue through the interaction between myocyte and adipocyte and identify important intermediary regulatory factors. Our proteomics data showed that the protein expression of tissue inhibitor of metalloproteinases 2(TIMP2) increased significantly in the culture medium of the co-culture system, and the content of lipid droplets was more in the co-culture intramuscular adipocytes. In addition, TIMP2 was significantly upregulated(P<0.01) in muscle tissue of individuals with high intramuscular fat *** gene co-expression network analysis revealed that TIMP2 was mainly involved in the extracellular matrix receptor interaction signaling pathway and its expression was significantly correlated with triglyceride, intramuscular fat,C14:0, C14:1, C16:0, C16:1, and C18:1n9C levels. Additionally, TIMP2 was co-expressed with various representative genes related to lipid metabolism(such as ADIPOQ, SCD, ELOVL5, ELOVL7, and LPL), as well as certain genes involved in extracellular matrix receptor interaction(such as COL1A2, COL4A2, COL5A1, COL6A1, and COL6A3), which are also significantly upregulated(P<0.05 or P<0.01) in muscle tissue of individuals with high intramuscular fat *** findings reveal that TIMP2 promotes intramuscular fat deposition in muscle tissue through the extracellular matrix receptor interaction signaling pathway.

关键词： TIMP2 ECM intramuscular adipocytes muscle satellite cells chicken

Transcriptome-based analysis of key genes and pathways affecting the linoleic acid content in chickens

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Journal of Integrative Agriculture 2023年第12期22卷 3744-3754页

作者： zhao Wen-juan YUAN Xiao-ya XIANG Hai MA Zheng CUI Huan-xian LI Hua zhao gui-ping Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding Foshan UniversityFoshan 528000P.R.China Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193P.R.China

Linoleic acid is an essential polyunsaturated fatty acid that cannot be synthesized by humans or animals themselves and can only be obtained *** amount of linoleic acid present has an impact on the quality and flavour of meat and indirectly affects consumer ***,the molecular mechanisms influencing the deposition of linoleic acid in organisms are not *** the molecular mechanisms of linoleic acid deposition are not well understood,to investigate the main effector genes affecting the linoleic acid content,this study aimed to screen for hub genes in slow-type yellow-feathered chickens by transcriptome sequencing(RNA-Seq)and weighted gene coexpression network analysis(WGCNA).We screened for candidate genes associated with the linoleic acid content in slow-type yellow-feathered broilers.A total of 399 Tiannong partridge chickens were slaughtered at 126 days of age,fatty acid levels were measured in pectoral muscle,and pectoral muscle tissue was collected for transcriptome *** sequencing results were combined with phenotypes for WGCNA to screen for candidate *** enrichment analysis was also performed on the genes that were significantly enriched in the modules with the highest correlation.A total of 13310 genes were identified after quality control of transcriptomic data from 399 pectoral muscle *** was performed,and a total of 26 modules were obtained,eight of which were highly correlated with the linoleic acid *** key genes,namely,MDH2,ATP5B,RPL7A and PDGFRA,were screened according to the criteria|GS|>0.2 and|MM|>*** functional enrichment results showed that the genes within the target modules were mainly enriched in metabolic *** this study,a large-sample-size transcriptome analysis revealed that metabolic pathways play an important role in the regulation of the linoleic acid content in Tiannong partridge chickens,and MDH2,ATP5B,RPL7A and PDGFRA were screened as important candidate

关键词： chicken linoleic acid transcriptome sequencing weighted gene coexpression network analysis(WGCNA) metabolic pathways

Potential regulation of linoleic acid and volatile organic compound contents in meat of chickens by PLCD1

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Journal of Integrative Agriculture 2023年第1期22卷 222-234页

作者： LIU Xiao-jing WANG Yong-li LIU Li LIU Lu zhao gui-ping WEN Jie JIA Ya-xiong CUI Huan-xian State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Omega-3(linolenic acid(ALA),docosapentaenoic acid,eicosapentaenoic acid)and omega-6(linoleic acid(LA),arachidonic acid)polyunsaturated fatty acids are essential for health and normal physiological functioning in *** we report a genome-wide association study(GWAS)on LA content in chicken *** 19 significant single nucleotide polymorphisms(SNPs)identified by the GWAS approach were annotated in VILL,PLCD1 and OXSR1 genes with highly polymorphic linkage blocks,and explained 4.5%of the phenotypic variation in the LA ***,the PLCD1 mRNA expression level was negatively correlated with the LA content,and significantly higher in chickens with low LA content than in those with high LA *** addition,PLCD1 was found to be involved in metabolic pathways,***,the LA content was correlated with volatile organic compounds(e.g.,octanal,etc.),but no relationship was found with intramuscular fat and triglycerides in chicken *** results indicated that there are key SNPs in PLCD1 that regulate the content of LA,and it has no significant effect on fat deposition,but may affect the content of volatile organic compounds(VOCs).

关键词： linoleic acid flavor quality intramuscular fat PLCD1 chicken meat

Immunogenetic basis of chicken’s heterophil to lymphocyte ratio revealed by genome-wide indel variants analysis

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Journal of Integrative Agriculture 2023年第9期22卷 2810-2823页

作者： ZHANG Jin WANG Jie WANG Qiao CUI Huan-xian DING Ji-qiang WANG Zi-xuan Mamadou Thiam LI Qing-he zhao gui-ping Institute of Animal Sciences Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Poultry Institute Shandong Academy of Agricultural SciencesJinan 250023P.R.China

Enhancing host immunity is an effective way to reduce morbidity in *** to lymphocyte ratio(H/L)is associated with host disease resistance in *** with different H/L levels show different disease ***,the utility of the H/L as an indicator of immune function needs to be further *** this study,a H/L directional breeding chicken line(Jingxing yellow chicken)was constructed,which has been bred for 12 *** compared the function of heterophils,and combined statistical analysis to explore the candidate genes and pathways related to H/*** oxidative burst function of the heterophils isolated from the H/L selection line(G12)was increased(P=0.044)compared to the non-selection line(NS).The 22.44 Mb genomic regions which annotated 300 protein-coding genes were selected in the genome of G9(n=92)compared to NS(n=92)based on a genome-wide selective *** selective regions were identified containing genes like interferon induced with helicase C domain 1(IFIH1)and moesin(MSN)associated with the intracellular receptor signaling pathway,C–C motif chemokine receptor 6(CCR6),dipeptidyl peptidase 4(DPP4)and hemolytic complement(HC)associated with the negative regulation of leukocyte chemotaxis and tight junction protein 1(TJP1)associated with actin cytoskeleton *** addition,45 genome-wide significant indels containing 29 protein-coding genes were also identified as associated with the H/L based on genome-wide association study(GWAS).The expression of protein tyrosine phosphatase non-receptor type 5(PTPN5)(r=0.75,P=0.033)and oxysterol binding protein like 5(OSBPL5)(r=0.89,P=0.0027)were positively correlated with H/*** to the high H/L group,the expressions of PTPN5 and OSBPL5 were decreased(P<0.05)in the low H/L group of Beijing you *** A/A allelic frequency of indel 5_13108985(P=3.85E–06)within OSBPL5 gradually increased from the NS to G5 and G9,and the individuals with A/A exhibited lower H/L t

关键词： H/L ratio heterophil indel selective sweep GWAS

Transcriptome analysis of the spleen of heterophils to lymphocytes ratio-selected chickens revealed their mechanism of differential resistance to Salmonella

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Journal of Integrative Agriculture 2022年第8期21卷 2372-2383页

作者： WANG Jie ZHANG Qi Astrid Lissette BARRETO SANCHEZ ZHU Bo WANG Qiao ZHENG Mai-qing LI Qing-he CUI Huan-xian WEN Jie zhao gui-ping State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Salmonella is one of the most common food-borne pathogens and its resistance in chicken can be improved through genetic *** heterophils/lymphocytes(H/L)ratio in the blood reflects the immune system status of *** compared the genome data and spleen transcriptomes between the H/L ratio-selected and non-selected chickens,after Salmonella infection,aiming to identify the key genes participating in the antibacterial activity in the *** results revealed that,the selected population had stronger(P<0.05)liver resistance to Salmonella typhimurium(ST)than the non-selected *** the selected and non-selected lines,the identified differentiation genes encode proteins involved in biological processes or metabolic pathways that included the TGF-beta signaling pathway,FoxO signaling pathway,and Salmonella infection *** results of the analysis of all identified differentially expressed genes(DEGs)of spleen revealed that the G protein-coupled receptor(GPCR)and insulin-like growth factor(IGF-I)signaling pathways were involved in the Salmonella infection *** analysis of DEGs and FST(fixation index),identified candidate genes involved in Salmonella infection pathway,such as GPR39,NTRK2,and *** extensive genomic changes highlight the polygenic genetic of the immune response in these chicken *** genes related to the immune performance are differentially expressed in the selected and non-selected lines and the selected lines has a higher resistance to Salmonella.

关键词： heterophils/lymphocytes selective sweep RNA-seq Salmonella typhimurium disease-resistance

Effect of feeding transgenic cry1Ab/cry1Ac rice on indices of immune function in broilers

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Journal of Integrative Agriculture 2016年第6期15卷 1355-1363页

作者： LIU Ran-ran zhao gui-ping ZHENG Mai-qing LIU Jie ZHANG Jing-jing LI Peng LI Qing-he FENG Jing-hai ZHANG Min-hong WEN Jie Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing 100193 P.R. China State Key Laboratory of Animal Nutrition Beijing 1O0193 P.R. China

The safety of feeding transgenic crylAb/crylAc rice （a genetically modified （GM） rice variety） to broilers was examined from an immunological perspective. HatchlingArborAcres chickens （240） were assigned to two dietary treatments （diets containing GM or non-GM rice） with 12 replicates per group and 10 birds per replicate. Traits were measured on one randomly selected bird from each replicate at d 21 and 42. The 42-d feeding trial revealed that crylAb/crylAc rice had no significant effect relative to non-GM rice on body weight and the immune organ indices. No significant pathological lesion in the spleen and bursa of Fabricius was found in the GM rice group. There were no significant differences in serum concentrations of immunoglobulin Y （IgY）, IgM, interleukin 4 （IL-4） and IL-6 between the two groups at d 21 or 42, except for IL-6 being higher （P〈0.05） in the GM- fed chickens at d 42. There were no differences in the T and B lymphocyte transformation rate and CD4＋/CD8＋ ratio between the two groups at d 42. Additionally, there was no significant difference between the two diets in expression of relevant genes viz. the major histocompatibility complex class II beta chain （BLB2）, interferon beta 1 （IFNβ）, tumour necrosis factor alpha-like （TNFa） and toll-like receptor 4 （TLR4）in the spleen and bursa of Fabricius. All the data demonstrated that transgenic crylAb/ crylAc rice had no adverse effect on these aspects of immune function of broilers during 42-d feeding trial. Transgenic rice was therefore indistinguishable from non-GM rice in terms of short-term feeding in chickens.

关键词： transgenic crylAb/crylAc gene rice broiler immune trait gene expression

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泡沫金属在冲击载荷下的动态压缩行为

爆炸与冲击 2014年第3期34卷 278-284页

作者：张健赵桂平卢天健西安交通大学机械结构强度与振动国家重点实验室陕西西安710049

基于微CT扫描影像信息,建立泡沫金属材料二维细观有限元模型,考虑不规则胞孔的不均匀分布,根据实验结果拟合孔壁材料的弹塑性本构参数。研究了泡沫金属在不同加载速度下的压缩变形机理,重点讨论泡沫金属中弹塑性波的传播、惯性效应和从... 详细信息

基于微CT扫描影像信息,建立泡沫金属材料二维细观有限元模型,考虑不规则胞孔的不均匀分布,根据实验结果拟合孔壁材料的弹塑性本构参数。研究了泡沫金属在不同加载速度下的压缩变形机理,重点讨论泡沫金属中弹塑性波的传播、惯性效应和从冲击端传递到静止端的应力变化特征。对于相对密度为0.3的泡沫铝,弹性波速约为5km/s,与孔壁材料的弹性波速相当,塑性波速表现为随着加载速度的增大而增大。在加载速度为50~100m/s间变形模式从准静态模式转变为动态模式,未发现明显的临界速度,动态锁死应变随着加载速度的增大而增大。由于塑性波发生反射,试件会发生二次压缩过程,相应地,静止端产生二次应力平台。受惯性作用的影响,二次应力平台也随着加载速度的增大而提高。

关键词：固体力学塑性波速细观有限元模型闭孔泡沫铝应力增强

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梯度泡沫金属的冲击吸能特性

工程力学 2016年第8期33卷 211-220页

作者：张健赵桂平卢天健西安交通大学航天航空学院机械结构强度与振动国家重点实验室西安710049 西安理工大学土木建筑工程学院西北旱区生态水利工程国家重点实验室培育基地西安710048

基于闭孔泡沫铝的显微CT扫描信息,考虑胞孔的不规则形貌及胞孔分布的不均匀性,以及胞孔尺寸和壁厚沿泡沫高度的梯度分布,建立了梯度泡沫金属材料的二维细观有限元模型,分析了梯度泡沫金属材料在动态压缩过程中的变形、塑性波的传播和能... 详细信息

基于闭孔泡沫铝的显微CT扫描信息,考虑胞孔的不规则形貌及胞孔分布的不均匀性,以及胞孔尺寸和壁厚沿泡沫高度的梯度分布,建立了梯度泡沫金属材料的二维细观有限元模型,分析了梯度泡沫金属材料在动态压缩过程中的变形、塑性波的传播和能量变化特征。对于平均相对密度0.3、平均梯度系数0.4的梯度泡沫铝,低速(10 m/s)加载时,梯度泡沫金属在变形的整个过程中吸收的总能量均低于均匀泡沫金属;高速加载时,梯度泡沫金属沿负梯度方向压缩的早期吸能比均匀泡沫金属有优势,而且速度越高,优势越明显。

关键词：闭孔泡沫铝动态压缩塑性波细观有限元模型梯度多孔材料

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华北克拉通燕山期岩浆活动的数值模拟

地球科学（中国地质大学学报） 2012年第S1期37卷 203-212页

作者：乔彦超刘翠赵桂萍石耀霖中国科学院研究生院计算地球动力学实验室北京100049 中国地质大学地质过程与矿产资源国家重点实验室北京100083 中国地质大学岩石圈构造深部过程及探测技术教育部重点实验室北京100083

为了定量给出华北克拉通燕山期岩浆活动的规模,在合理构建华北燕山期地质-物理模型的基础上,利用有限元方法,在忽略玄武岩浆内对流只考虑热传导的假定下,反演了该区燕山期形成花岗岩所需要的侵入玄武岩浆的量.通过计算表明:(1)在压力1GP... 详细信息

为了定量给出华北克拉通燕山期岩浆活动的规模,在合理构建华北燕山期地质-物理模型的基础上,利用有限元方法,在忽略玄武岩浆内对流只考虑热传导的假定下,反演了该区燕山期形成花岗岩所需要的侵入玄武岩浆的量.通过计算表明:(1)在压力1GPa、地幔的玄武岩浆底侵作用于下地壳英云闪长岩围岩的情况下,英云闪长岩围岩的纯熔融量与底侵玄武岩纯结晶总量的比值约为0.161;假设仅有25%的熔浆可分离出来,则分离出的熔浆与玄武岩结晶总量比约为0.04025.(2)数值模拟得到最后发生相变的整体区域为-50.285～-36.867km.与前人研究得到的现今下地壳下部和壳幔过渡带33～40m和40～50km接近,表明此数值模拟方法具有可行性.

关键词：华北燕山期相变热传导岩浆活动地球物理岩石圈