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Development and Characterization of an Electrochemical Sensor for Cinchonidine Detection Based on Molecularly Imprinted Polymer with Modified Rosin as Cross-linker

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Chemical Research in Chinese Universities 2012年第3期28卷 410-414页

作者： LIU Li TAN Xue-cai zhao dan-dan WANG Lin LEI Fu-hou HUANG Zai-yin School of Chemistry and Ecological Engineering Guangxi University for NationalitiesNanning 530008P.R.China Guangxi Key Laboratory of Chemistry and Engineering of Forest Produts Guangxi University for NationalitiesNanning 530008P.R.China Key Laboratory of Chemical and Biological Transforming Process Guangxi UniversityNanning 530008P.R.China

Molecularly imprinted polymers（MIPs） were applied as molecular recognition elements to an electro- chemical sensor for cinchonidine（CD）. A kind of MIP was synthesized with cinchonidine as template, modified ro- sin（ethylene glycol maleic rosinate acrylate） containing the skeleton of phenanthrene rings as cross-linker and me- thylacrylic acid as functional monomer. MIP membrane was prepared on a glassy carbon electrode for the determina- tion of CD via free radical polymerization method. Electrochemical impedance spectroscopy（EIS） and cyclic vol- tammetry（CV） were used to characterize the membrane electrochemical behavior in electrode fabrication process. The experimental conditions were discussed. Under optimum conditions, it was found that the response of peak cur- rents was linear to the concentration of CD in a range of 0.013-2.26 mmol/L. The detection limit for CD is 1 μmol/L the relative standard deviation for 100 μmol/L CD is 1.34% and the incubation time is 2 min. The sensor was applied to the determination of CD in urine samples with satisfactory results.

关键词： Ethylene glycol maleic rosinate acrylate Cinchonidine Molecularly imprinted polymer Electrochemicalsensor

Fe_3O_4 Magnetic Nanoparticles Modified Electrode as a Sensor for Determination of Nimesulide

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Chemical Research in Chinese Universities 2011年第4期27卷 566-569页

作者： ZHANG Jin-lei TAN Xue-cai zhao dan-dan TAN Sheng-wei LIU Li WANG Lin HUANG Zeng-wei College of Chemistry and Ecological Engineering Guangxi University for Nationalities Nanning 530006 P. R. China Department of Chemistry and Life Science Baise University Baise 533000 P. R. China

A novel type of Fe3O4 nanoparticles modified glass carbon electrode（Fe3O4/GCE） was constructed and the electrochemical properties of N-（4-nitro-2-phenoxyphenyl）methanesulfonamide（nimesulide） were studied on the Fe3O4/*** 0.4mol/L HAc-NaAc buffer solution（pH=5.0）,the electrode process of nimesulide was irreversible at bare GCE and Fe3O4/*** Fe3O4/GCE exhibited a remarkable catalytic and enhancement effect on the reduction of *** reduction peak potential of nimesulide shifted positively from-0.683 V at bare GCE to-0.625 V at Fe3O4/GCE,and the sensitivity was increased by ca.3 *** experimental conditions were *** linear range between the peak current and the concentration of nimesulide was 2.6×10-6 ＂1.0×10-4mol/L（R=0.993） with a detection limit of 1.3×10-7mol/*** method has been used to determine the content of nimesulide in medical *** recovery was determined to be 96.9% ＂101.9% by means of standard addition *** method is comparable to UV-Vis spectrometry.

关键词： Fe3O4 nanoparticle Modified electrode Nimesulide Determination Electrochemical behavior

Amperometric Hydrogen Peroxide Biosensor Based on Multiwall Carbon Nanotubes and Cadmium Sulfide Quantum Dots

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Chemical Research in Chinese Universities 2010年第4期26卷 541-545页

作者： ZHANG Jin-lei TAN Xue-cai zhao dan-dan TAN Sheng-wei HUANG Zeng-wei MI Yan HUANG Zai-yin College of Chemistry and Ecological Engineering Guangxi University for Nationalities Nanning 530006 P. R. China Department of Chemistry and Life Science Baise University Baise 533000 P. R. China

A novel third-generation hydrogen peroxide（H2O2） biosensor（Hb/CdS/MWNTs/GCE） was fabricated through hemoglobin（Hb） adsorbed onto the mercaptoacetic acid modified CdS QDs/carboxyl multiwall carbon nanotubes＇（MWNTs） films. Cyclic voltammogram of Hb/CdS/MWNTs/GCE showed a pair of well-defined and quasi-reversible redox peaks with a formal potential（E^0） of-0.230 V（vs. Ag/AgCl） in 0.1 mol/L pH=8.0 phosphate buffer solution（PBS）, which was the characteristic of the Hb heme Fe（Ⅲ）/Fe（Ⅱ） redox couple. The biosensor shows an excellent electrocatalytic activity to the reduction of H2O2. The response time of the designed biosensor to H202 at a potential of-0.30 V was less than 2 s and linear relationships were obtained in the concentration ranges of 2.0×10^-6-2.7×10^-3 mol/L and 2.7×10^-3-7.7×10^-3 mol/L with a detection limit of 3.0×10^-7 mol/L（S/N=3）. The apparent Michaelis-Menten constant Km was estimated to be 1.324 mmol/L that illustrated the excellent biological activity of the fixed Hb.

关键词： Hemoglobin CdS quantum dot Multi-wall carbon nanotube Biosensor Direct electrochemistry

Generation of recombinant rabies virus ERA strain applied to virus tracking in cell infection

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Journal of Integrative Agriculture 2019年第10期18卷 2361-2368页

作者： zhao dan-dan SHUAI Lei GE Jin-ying WANG Jin-liang WEN Zhi-yuan LIU Ren-qiang WANG Chong WANG Xi-jun BU Zhi-gao State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhou 225009P.R.China

The mechanism of rabies virus (RABV) infection still needs to be further characterized. RABV particle with self-fluorescent is a powerful viral model to visualize the viral infection process in cells. Herein, based on a reverse genetic system of the Evelyn-Rokitnicki-Abelseth (rERA) strain, we generated a recombinant RABV rERA-N/mCherry strain that stably expresses an additional ERA nucleoprotein that fuses with the red fluorescent protein mCherry (N/mCherry). The rERA-N/mCherry strain retained growth property similar to the parent strain rERA in vitro. The N/mCherry expression showed genetic stability during passage into mouse neuroblastoma (NA) cells and did not change the virulence of the vector. The rERA-N/mCherry strain was then utilized as a visual viral model to study the RABV-cell binding and internalization. We directly observed the red self-fluorescence of rERA-N/mCherry particles binding to the cell surface, and further co-localizing with clathrin in the early stage of infection in NA cells by fluorescence microscopy. Our results showed that the rERA-N/mCherry strain uses clathrin-dependent endocytosis to enter cells, which is consistent with the well-known mechanism of RABV invasion. The recombinant RABV rERA-N/mCherry thus appears to have the potential to be an effective viral model to further explore the fundamental molecular mechanism of rabies neuropathogenesis.

关键词： rabies virus self-fluorescence binding internalization

Berberine Induces Cell Apoptosis through Cytochrome C/Apoptotic Protease-Activating Factor 1/Caspase-3 and Apoptosis Inducing Factor Pathway in Mouse Insulinoma Cells

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Chinese Journal of Integrative Medicine 2019年第11期25卷 853-860页

作者： FANG Xin MIAO Xiao-liang LIU Jun-li ZHANG Dong-wei WANG Min zhao dan-dan MU Qian-qian YU Na MO Fang-fang YIN Hong-ping GAO Si-hua School of Basic Medicine Beijing University of Chinese MedicineBeijing (100029)China Fraser Laboratories for Diabetes Research Department of MedicineRI-McGill University Health CentreMontrealQuebec (H4A3J1)Canada School of Life Science and Tech no logy China Pharmaceutical Un iversityNanjing (210009)China Diabetes Research Center Beijing University of Chinese MedicineBeijing (100029)China

Objective:To investigate apoptotic effects of berberine,a significant alkaloids component existing in Rhizoma coptidis,and its possible acting mechanism in insulinoma ***:Different concentrations of berberine were used to treat mouse insulinoma(MIN6)cells for various period of *** viability and apoptosis of the cells were analyzed using methylthiazolyldiphenvl-tetrazolium bromide assay,flow cytometry and enzyme-linked immuno sorbent *** in the relating pro-and anti-apoptosis proteins were detected by ***:The half-maximal inhibitory concentration(IC50)of berberine was 5.7μmol/L on MIN6 cells viability for 16 *** caused a 20%reduction(P<0.05)in cell number after only 4-h incubation;which reached 50%after 24 h(P<0.01).Berberine treatment for 16 h significantly increased the level of DNA *** flow cytometry showed the apoptotic rate increased 2.9-and 4.6-fold after treating with berberine(5μmol/L)for 8 and 16 h,while 3-and 8.7-fold after 10μmol/L treatment for 8 and 16 h(P<0.01).Berberine treatment dramatically elevated the expression ratio of Bax to ***,berberine notably increased the apoptosis-inducing factors and cytochrome C transforming from the mitochondria to the *** protease-activating factor 1(Apaf-1)was subsequently activated after cytochrome C ***,caspase-3 and poly adenosine diphosphate-ribose polymerase were also activated to trigger apoptosis ***:High concentration(5 and 10μmol/L)of berberine could induce the apoptosis of MIN6 cells through cytochrome C/Apaf-1/caspase-3 and apoptosis inducing factor(AIF)pathway.

关键词： Chinese medicine berberine apoptosis mouse insulinoma cells cytochrome C/Apaf-1/caspase-3 apoptosis inducing factor pathway

基于灰色关联分析的35CrMoV钢活塞杆激光熔覆工艺参数优化

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兵工学报 2018年第10期39卷 2073-2080页

作者：赵丹丹焦锋河南理工大学机械与动力工程学院河南焦作454000

为选取优化合理的激光熔覆工艺参数匹配,获得致密性好、耐磨性强的熔覆层,采用激光熔覆技术对基材为35CrMoV钢的已磨损表面进行修复试验。以激光扫描速度、激光熔覆功率、送粉速度为自变量,以正交试验数据为基础,基于灰色系统的理论模... 详细信息

为选取优化合理的激光熔覆工艺参数匹配,获得致密性好、耐磨性强的熔覆层,采用激光熔覆技术对基材为35CrMoV钢的已磨损表面进行修复试验。以激光扫描速度、激光熔覆功率、送粉速度为自变量,以正交试验数据为基础,基于灰色系统的理论模型对试验数据进行处理;通过层次分析法计算多目标权重,运用灰色关联理论,分别计算并得到各工艺参数对理想单目标值的关联系数及对多目标值的关联度;将单目标参数优化转换为综合工艺参数优化,获得了优化工艺参数组合:扫描线速度100 mm/s、送粉速度42. 8 g/min、激光熔覆功率1. 8 kW;通过试验对优化结果进行了检验,结果表明:在最优工艺参数条件下,熔覆层质量得到明显提高,特别是耐磨性相对提高了43%.

关键词： 35CrMoV钢活塞杆激光熔覆工艺参数磨损率关联度

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钛合金在航空领域的发展与应用

铸造 2014年第11期63卷 1114-1117页

作者：赵丹丹哈尔滨金融学院黑龙江哈尔滨150030

钛合金具有较高的比强度和较好的高温强度,广泛应用于航空领域飞机机身结构材料和航空发动机材料。介绍了目前钛合金在航空领域的应用现状,重点介绍了高性能钛合金的研究现状,包括高强度钛合金、高温钛合金以及阻燃钛合金。最后总结归... 详细信息

钛合金具有较高的比强度和较好的高温强度,广泛应用于航空领域飞机机身结构材料和航空发动机材料。介绍了目前钛合金在航空领域的应用现状,重点介绍了高性能钛合金的研究现状,包括高强度钛合金、高温钛合金以及阻燃钛合金。最后总结归纳了我国航空钛合金的发展趋势。

关键词：钛合金航空应用现状

Activated mTOR signaling pathway in myofibers with inherited metabolic defect might be an evidence for mTOR inhibition therapies

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Chinese Medical Journal 2019年第7期132卷 805-810页

作者： Jing-Wei Lyu Xue-Bi Xu Kun-Qian Ji Na Zhang Yuan Sun dan-dan zhao Yu-Ying zhao Chuan-Zhu Yan Institute of Neuromuscular and Neurodegenerative Diseases Department of NeurologyQilu HospitalShandong UniversityJinanShandong 250012China Brain Science Research Institute Shandong UniversityJinanShandong 250012China Department of Neurology Qilu Hospital (Qingdao)Shandong UniversityQingdaoShandong 266035China Mitochondrial Medicine Laboratory Qilu Hospital (Qingdao)Shandong UniversityQingdaoShandong 266035China

Background:Abnormally activated mechanistic target of rapamycin(mTOR)pathway has been reported in several model animals with inherited metabolic myopathies(IMMs).However,the profiles of mTOR pathway in skeletal muscles from patients are still *** study aimed to analyze the activity of mTOR pathway in IMMs ***:We collected muscle samples from 25 patients with mitochondrial myopathy(MM),lipid storage disease(LSD)or Pompe disease(PD).To evaluate the activity of mTOR pathway in muscle specimens,phosphorylation of S6 ribosomal protein(p-S6)and p70S6 kinase(p-p70S6K)were analyzed by Western blotting and ***:Western blotting results showed that p-p70S6K/p70S6K in muscles from LSD and MM was up-regulated when compared with normal controls(NC)(NC ***,U=2.000,P=0.024;NC ***:U=6.000,P=0.043).Likewise,p-S6/S6 was also upregulated in muscles from all three subgroups of IMMs(NC ***,U=0.000,P=0.006;NC ***,[7=0.000,P=0.006;NC ***,U=1.000,P=0.007).Immunohistochemical study revealed that p-S6 was mainly expressed in fibers with metabolic *** MM muscles,most p-S6 positive fibers showed cytochrome C oxidase(COX)deficiency(U=5.000,P=0.001).In LSD and PD muscles,p-S6 was mainly overexpressed in fibers with intramuscular vacuoles containing lipid droplets(17=0.000,P=0.002)or basophilic materials(U=0.000,P=0.002).Conclusion:The mTOR pathway might be activated in myofibers with various metabolic defects,which might provide evidence for mTOR inhibition therapy in human IMMs.

关键词： mTOR pathway Mitochondrial myopathy Lipid storage disease Pompe disease

禀赋特征、外部性与农业生产集聚:基于全国31省的证据

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长江流域资源与环境 2019年第9期28卷 2130-2140页

作者：赵丹丹周宏金陵科技学院商学院江苏南京211169 南京农业大学粮食经济研究中心江苏南京210095

在农业资源不断匮乏背景下,我国农业表现出生产集聚现象,从集聚效应视角看,农业生产集聚的形成是资源禀赋、外部性因素互相作用的结果。本文系统梳理了资源禀赋特征、外部性条件与农业生产集聚之间内在机制,并利用我国1996~2015年31省2... 详细信息

在农业资源不断匮乏背景下,我国农业表现出生产集聚现象,从集聚效应视角看,农业生产集聚的形成是资源禀赋、外部性因素互相作用的结果。本文系统梳理了资源禀赋特征、外部性条件与农业生产集聚之间内在机制,并利用我国1996~2015年31省20年面板数据,通过固定效应模型和系统广义矩估计等多种研究方法进行验证,通过理论分析和回归分析发现:(1)农业资源禀赋中农业劳动力、农业资本存量、农业技术水平和耕地资源对生产集聚水平的提高具有显著的促进作用。而外部性条件中自然灾害和地形对农业生产集聚水平具有显著的制约作用。(2)从影响趋势上来看,农业耕地数量、农业技术水平对农业生产集聚水平的影响表现出'U'型发展趋势,而农业劳动力数量和农业资本存量表现出倒'U'型发现趋势。

关键词：供给侧资源禀赋生产集聚集聚效应