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Conditional reprogramming:next generation cell culture

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Acta Pharmaceutica Sinica B 2020年第8期10卷 1360-1381页

作者： Xiaoxiao Wu Shengpeng Wang Mingxing Li Jing Li Jing Shen Yueshui zhao jun Pang Qinglian Wen Meijuan Chen Bin Wei Parham Jabbarzadeh Kaboli Fukuan Du Qijie zhao Chi Hin Cho Yitao Wang Zhangang Xiao Xu Wu Laboratory of Molecular Pharmacology Department of PharmacologySchool of PharmacySouthwest Medical UniversityLuzhou 646000China South Sichuan Institute of Translational Medicine Luzhou 646000China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacaoChina Department of Oncology and Hematology Hospital(T.C.M)Affiliated to Southwest Medical UniversityLuzhou 646000China Center of Radiation Oncology Hospital(T.C.M)Affiliated to Southwest Medical UniversityLuzhou 646000China Department of Oncology the Affiliated Hospital of Southwest Medical UniversitySouthwest Medical UniversityLuzhou 646000China College of Pharmaceutical Science&Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals Zhejiang University of TechnologyHangzhou 310014China School of Biomedical Sciences Faculty of Medicinethe Chinese University of Hong KongHong KongChina

Long-term primary culture of mammalian cells has been always difficult due to unavoidable senescence. Conventional methods for generating immortalized cell lines usually require manipulation of genome which leads to change of important biological and genetic characteristics. Recently, conditional reprogramming(CR) emerges as a novel next generation tool for long-term culture of primary epithelium cells derived from almost all origins without alteration of genetic background of primary cells. CR cocultures primary cells with inactivated mouse 3T3-J2 fibroblasts in the presence of RHO-related protein kinase(ROCK) inhibitor Y-27632, enabling primary cells to acquire stem-like characteristics while retain their ability to fully differentiate. With only a few years’ development, CR shows broad prospects in applications in varied areas including disease modeling, regenerative medicine, drug evaluation, drug discovery as well as precision medicine. This review is thus to comprehensively summarize and assess current progress in understanding mechanism of CR and its wide applications, highlighting the value of CR in both basic and translational researches and discussing the challenges faced with CR.

关键词： culture epithelium programming

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基于阶段耦合的项目组合配置战略贴近度

中国管理科学 2017年第3期25卷 68-75页

作者：白礼彪白思俊张以彬长安大学经济与管理学院陕西西安710061 北京航空航天大学经济管理学院北京100191 西北工业大学管理学院陕西西安710072 上海立信会计学院工商管理学院上海201620

论文以项目组合配置阶段界定为研究出发点,在分析项目组合配置多阶段过渡耦合模式的基础上,提出了以阶段内所有拟配置组件战略属性承载值加权和度量阶段内项目组合配置战略贴近度、紧前阶段释放对象有效利用率反映阶段过渡耦合度的分析... 详细信息

论文以项目组合配置阶段界定为研究出发点,在分析项目组合配置多阶段过渡耦合模式的基础上,提出了以阶段内所有拟配置组件战略属性承载值加权和度量阶段内项目组合配置战略贴近度、紧前阶段释放对象有效利用率反映阶段过渡耦合度的分析方法,构建了基于阶段耦合的项目组合配置战略贴近度模型,并通过企业实践,对模型的可操作性进行了验证。

关键词：阶段界定阶段耦合项目组合配置战略贴近度

Discontinuous polyostotic fibrous dysplasia with multiple systemic disorders and unique genetic mutations:A case report

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World Journal of Clinical Cases 2020年第23期8卷 6197-6205页

作者： Tiao Lin Xin-Yu Li Chang-Ye Zou Wei-Wei Liu jun-Fan Lin Xin-Xin Zhang Si-Qi zhao Xian-Biao Xie Gang Huang jun-Qiang Yin Jing-Nan Shen Department of Musculoskeletal Oncology The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080Guangdong ProvinceChina Zhongshan School of Medicine Sun Yat-sen UniversityGuangzhou 510080Guangdong ProvinceChina

BACKGROUND Polyostotic fibrous dysplasia(PFD)is an uncommon developmental bone disease in which normal bone and marrow are replaced by pseudotumoral *** etiology of PFD is unclear,but it is generally thought to be caused by sporadic,post-zygotic mutations in the GNAS ***,we report the case of a young female with bone pain and lesions consistent with PFD,unique physical findings,and gene *** SUMMARY A 27-year-old female presented with unbearable bone pain in her left foot for 4 *** bone lesions were detected by radiographic examinations,and a diagnosis of PFD was made after a biopsy of her left calcaneus with symptoms including pre-axial polydactyly on her left hand and severe ophthalmological problems such as high myopia,vitreous opacity,and choroidal *** serum cortisol level was high,consistent with Cushing *** to consanguineous marriage of her grandparents,boosted whole exome screening was performed to identify gene *** results revealed mutations in HSPG2 and RIMS1,which may be contributing factors to her unique *** The unique findings in this patient with PFD may be related to mutations in the HSPG2 and RIMS1 genes.

关键词： Polyostotic fibrous dysplasia Genetic mutation Hypercortisolism Drug resistance Ophthalmological problems Case report

Small molecule deoxynyboquinone triggers alkylation and ubiquitination of Keap1 at Cys489 on Kelch domain for Nrf2 activation and inflammatory therapy

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Journal of Pharmaceutical Analysis 2024年第3期14卷 401-415页

作者： Ke-Gang Linghu Tian Zhang Guang-Tao Zhang Peng Lv Wen-jun Zhang Guan-Ding zhao Shi-Hang Xiong Qiu-Shuo Ma Ming-Ming zhao Meiwan Chen Yuan-Jia Hu Chang-Sheng Zhang Hua Yu State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao SARChina Macao Centre for Research and Development in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao SARChina Key Laboratory of Tropical Marine Bio-resources and Ecology Guangdong Key Laboratory of Marine Materia MedicaRNAM Center for Marine MicrobiologySouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou510301China State Key Laboratory of Functions and Applications of Medicinal Plants&School of Pharmaceutical Sciences Guizhou Medical UniversityGuiyang550025China

Activation of nuclear factor erythroid 2-related factor 2(Nrf2)by Kelch-like ECH-associated protein 1(Keap1)alkylation plays a central role in anti-inflammatory ***,activators of Nrf2 through alkylation of Keap1-Kelch domain have not been ***(DNQ)is a natural small molecule discovered from marine *** current study was designed to investigate the anti-inflammatory effects and molecular mechanisms of DNQ via alkylation of *** exhibited significant anti-inflammatory properties both in vitro and in *** pharmacophore responsible for the anti-inflammatory properties of DNQ was determined to be theα,β-unsaturated amides moieties by a chemical reaction between DNQ and *** exerted anti-inflammatory effects through activation of Nrf2/ARE ***1 was demonstrated to be the direct target of DNQ and bound with DNQ through conjugate addition reaction involving *** specific alkylation site of DNQ on Keap1 for Nrf2 activation was elucidated with a synthesized probe in conjunction with liquid chromatography-tandem mass *** triggered the ubiquitination and subsequent degradation of Keap1 by alkylation of the cysteine residue 489(Cys489)on Keap1-Kelch domain,ultimately enabling the activation of *** findings revealed that DNQ exhibited potent anti-inflammatory capacity throughα,β-unsaturated amides moieties active group which specifically activated Nrf2 signal pathway via alkylation/ubiquitination of Keap1-Kelch domain,suggesting the potential values of targeting Cys489 on Keap1-Kelch domain by DNQ-like small molecules in inflammatory therapies.

关键词： Deoxynyboquinone Anti-inflammation Target Keap1/Nrf2 Alkylation Ubiquitination

Hierarchical ferric-cobalt-nickel ternary oxide nanowire arrays supported on graphene fibers as high-performance electrodes for flexible asymmetric supercapacitors

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Nano Research 2018年第4期11卷 1775-1786页

作者： Jingxin zhao Chaowei Li Qichong Zhang jun Zhang Xiaona Wang Juan Sun Juanjuan Wang Jixun Xie Ziyin Lin Zhuo Li Weibang Lu Conghua Lu Yagang Yao School of Materials Science and Engineering Tianjin University Tianjin 300072 China Division of Advanced Nanomaterials Key Laboratory of Nanodevices and Applications Suzhou Institute of Nano-tech and Nano-bionics Chinese Academy of Sciences University of Chinese Academy of Sciences Suzhou 215123 China School of Materials Science and Engineering Georgia Institute of Technology 771 Ferst Drive Atlanta 30332 USA

Fiber-based supercapacitors （FSCs） are new members of the energy storage family. They present excellent flexibility and have promising applications in lightweight, flexible, and wearable devices. One of the existing challenges of FSCs is enhancing their energy density while retaining the flexibility. We developed a facile and cost-effective method to fabricate a highly capacitive positive electrode based on hierarchical ferric-cobalt-nickel ternary oxide nanowire arrays/graphene fibers and a negative electrode based on polyaniline-derived carbon nanorods/graphene fibers. The elegant microstructures and excellent electrochemical performances of both electrodes enabled us to construct a high- performance flexible asymmetric graphene fiber-based supercapacitor device with an operating voltage of 1.4 V, a specific capacitance up to 61.58 ***-2, and an energy density reaching 16.76 μW·h·cm-2. Moreover, the optimal device presents an outstanding cycling stability with 87.5% initial capacitance retention after 8,000 cycles, and an excellent flexibility with a capacitance retention of 90.9% after 4,000 cycles of repetitive bending.

关键词： fiber-based supercapacitors ferric-cobalt-nickel ternary oxide nanowire arrays polyaniline-derived carbon nanorods flexibility

Soliton propagation for a coupled Schrödinger equation describing Rossby waves

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Chinese Physics B 2023年第7期32卷 206-215页

作者：徐丽阳尹晓军曹娜白淑婷 College of Science Inner Mongolia Agriculture UniversityHohhot 010018China

We study a coupled Schrödinger equation which is started from the Boussinesq equation of atmospheric gravity waves by using multiscale analysis and reduced perturbation *** the coupled Schrödinger equation,we obtain the Manakov model of all-focusing,all-defocusing and mixed types by setting parameters value and apply the Hirota bilinear approach to provide the two-soliton and three-soliton ***,we find that the all-defocusing type Manakov model admits bright-bright soliton ***,we find that the all-defocusing type Manakov model admits bright-bright-bright soliton ***,we go over how the free parameters affect the Manakov model’s allfocusing type’s two-soliton and three-soliton solutions’collision locations,propagation directions,and wave *** findings are useful for setting a simulation scene in Rossby waves *** answers we have found are helpful for studying physical properties of the equation in Rossby waves.

关键词： Hirota bilinear method Schrödinger equation soliton solution Rossby waves

Establishment of a Predictive Model Related to Pathogen Invasion for Infectious Diseases and Its Diagnostic Value in Fever of Unknown Origin

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Current Medical Science 2018年第6期38卷 1025-1031页

作者： Zhu-hua WU Ming-you XING Sheng WEI Man-zhi zhao Wen-xia WANG Lin ZHU Ji-ling ZHU Cai-feng ZHENG Si-jun WANG jun-ying QI Jian-xin SONG Department of Infectious Diseases Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030China Department of Epidemiology and Biostatistics School of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Oncology the Third Affiliated Hospital of Xinxiang Medical UniversityXinxiang 453000China

The present study aimed to establish a list of parameters indicative of pathogen invasion and develop a predictive model to distinguish the etiologies of fever of unknown origin (FUO) into infectious and non-infectious *** January 2014 to September 2017,431 patients with FUO were prospectively enrolled in the study *** study established a list of 26 variables from the following 4aspects:host factors,epidemiological factors,behavioral factors,and iatrogenic *** predicted variables were included in a multivariate logistic regression analysis to develop a predictive *** predictive model and the corresponding scoring system were developed using data from the confirmed diagnoses and 9 variables were eventually *** factors were incorporated into the predictive *** model discriminated between infectious and non-infectious causes of FUO with an AUC of 0.72,sensitivity of 0.71, and specificity of *** predictive model and corresponding scoring system based on factors concerning pathogen invasion appear to be reliable screening tools to discriminate between infectious and non-infectious causes of FUO.

关键词： fever of unknown origin predictive model etiology empiric therapy

Three-dimensional Culture of Human Airway Epithelium in Matrigel for Evaluation of Human Rhinovirus C and Bocavirus Infections

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Biomedical and Environmental Sciences 2018年第2期31卷 136-145页

作者： CHEN Ya Xiong XIE Guang Cheng PAN Dong DU Ya Rong PANG Li Li SONG Jing Dong DUAN zhao jun HU Bu Rong Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences & Key Laboratory of Space Radiobiology of Gansu Province Institute of Modern Physics Chinese Academy of Sciences Department of Pathogenic Biology Chengde Medical University Department for Viral Diarrhea National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

Objective Newly identified human rhinovirus C （HRV-C） and human bocavirus （HBoV） cannot propagate in vitro in traditional cell culture models; thus obtaining knowledge about these viruses and developing related vaccines are difficult. Therefore, it is necessary to develop a novel platform for the propagation of these types of *** A platform for culturing human airway epithelia in a three-dimensional （3D） pattern using Matrigel as scaffold was developed. The features of 3D culture were identified by immunochemical staining and transmission electron microscopy. Nucleic acid levels of HRV-C and HBoV in 3D cells at designated time points were quantitated by real-time polymerase chain reaction {PCR）. Levels of cytokines, whose secretion was induced by the viruses, were measured by *** Properties of bronchial-like tissues, such as the expression of biomarkers CK5, ZO-2, and PCK, and the development of cilium-like protuberances indicative of the human respiration tract, were observed in 3D-cultured human airway epithelial （HAE） cultures, but not in monolayer-cultured cells. Nucleic acid levels of HRV-C and HBoV and levels of virus-induced cytokines were also measured using the 3D culture *** Our data provide a preliminary indication that the 3D culture model of primary epithelia using a Matrigel scaffold in vitro can be used to propagate HRV-C and HBoV.

关键词： 3D cell culture Human airway epithelium (HAE) Human rhinovirus C Human bocavirus Propagation

Two Preparation Methods-dominated Cd^(Ⅱ)-Based Coordination Polymers with Mixed Adenine Nucleobase and 5-Nitroisophathalate Ligands: Synthesis, Structure and Fluorescence

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Chinese Journal of Structural Chemistry 2019年第4期38卷 613-620页

作者： LIU Zheng-Yu FU Min WANG Xiu-Guang WANG Li-Li YANG En-Cui zhao Xiao-jun College of Chemistry Key Laboratory of Inorganic-Organic Hybrid Functional Material ChemistryMinistry of Education Tianjin Key Laboratory of Structure and Performance for Functional Molecules Tianjin Normal University

Two new CdⅡ-based coordination polymers(CPs) with mixed adenine(Hade) nucleobase and 5-nitroisophathalate(nip) ligands, {[Cd(Hade)_(0.5)(H_2O)_2(nip)]·H_2O}n 1 and {[Cd(Hade)(H_2O)_(1.25)(CH_3OH)0.75(nip)]·0.75 CH_3OH·0.5H_2 O}_n 2, were successfully obtained by varying the preparation methods and structurally characterized. Crystal data for 1: monoclinic, C2/c space group with a = 10.5546(6), b = 17.3496(6), c = 16.1198(9) ?, β = 104.2800(10)o, V = 2860.6(2) ?3, Dc = 2.058 g/cm^3, Mr = 443.13, Z = 8, F(000) = 1752, μ = 1.585 mm^(–1), the final R = 0.0394 and wR = 0.1109 for 2285 observed reflections with I > 2σ(I). For 2: triclinic, P1 space group with a = 10.2032(7), b = 10.5098(8), c = 11.0223(8) ?, a = 65.7050(10)o, β = 74.5750(10)o, g = 61.5280(10)o, V = 943.38(12) ?~3, Dc = 1.888 g/cm3, Mr = 536.24, Z = 2, F(000) = 537, μ = 1.225 mm^(–1), the final R = 0.0225 and wR = 0.0702 for 3143 observed reflections with I > 2σ(I). 1 presents a crisscrossed layer with mutually orthogonal {Cd(nip)} chains aggregated by neutral m-N(7),N(9)-Hade connector. By contrast, 2 displays a linear chain with CdⅡ ions extended by bis-bidentate chelating-nip2–connectors, which are further assembled into a broad ribbon by N-H···N hydrogen-bonding interactions. Additionally, the two solid-state samples with comparable thermal stability exhibit favorable luminescent emissions at room temperature, suggesting their potential applications as fluorescence materials.

关键词： adenine nucleobase coordination polymer crystal structure fluorescence