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p53活性四聚体全原子分子动力学分析

物理学报 2024年第4期73卷 300-313页

作者：周晗耿轶钊晏世伟北京师范大学物理学系北京100875 河北工业大学理学院天津300131 北京师范大学文理学院珠海519085

p53是一种肿瘤抑制蛋白,对阻碍癌症发展、维持遗传完整性起着至关重要的作用.在细胞核内,4个p53分子通过高度协同的方式、通过DNA结合域与DNA结合,形成稳定的四聚体活性结构,并转录激活或抑制其靶向基因.然而,大多数肿瘤细胞中存在大量... 详细信息

p53是一种肿瘤抑制蛋白,对阻碍癌症发展、维持遗传完整性起着至关重要的作用.在细胞核内,4个p53分子通过高度协同的方式、通过DNA结合域与DNA结合,形成稳定的四聚体活性结构,并转录激活或抑制其靶向基因.然而,大多数肿瘤细胞中存在大量p53的突变,其中绝大部分突变发生在p53的DNA结合域,而p53的DNA结合域又是p53形成四聚体活性结构、调控下游靶基因转录的重要区域.本文通过全原子分子动力学模拟,研究了野生型p53四聚体内分子间的相互作用机制.结果表明,位于DNA两侧的对称二聚体是一个稳定的二聚体,在与DNA结合前后都能维持稳定的结构.位于DNA同侧的两个单体依靠两个接触面提供的蛋白-蛋白相互作用和DNA的骨架作用使四聚体活性结构保持稳定,这些相互作用为四聚体的形成机制提供了重要支撑.该工作厘清了p53四聚体在动力学过程中的内部相互作用机制和关键残基,揭示了四聚化过程中各个相互作用界面的关键位点,对于理解p53的抑癌机制、探索有效治癌策略、发展治癌药物具有重要意义.

关键词： p53核心四聚体 p53核心结构域蛋白-蛋白相互作用分子动力学模拟

AtVQ25 promotes salicylic acid-related leaf senescence by fine-tuning the self-repression of AtWRKY53

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Journal of Integrative Plant Biology 2024年第6期66卷 1126-1147页

作者： Qi Tan Mingming zhao Jingwei Gao Ke Li Mengwei Zhang Yunjia Li Zeting Liu Yujia Song Xiaoyue Lu Zhengge Zhu Rongcheng Lin Pengcheng Yin Chunjiang Zhou geng Wang Ministry of Education Key Laboratory of Molecular and Cellular Biology Hebei Research Center of the Basic Discipline of Cell BiologyHebei Collaboration Innovation Center for Cell Signaling and Environmental AdaptationHebei Key Laboratory of Molecular and Cellular BiologyCollege of Life SciencesHebei Normal UniversityShijiazhuang 050024China College of Chemical Engineering Shijiazhuang UniversityShijiazhuang 050035China Institute of Botany Chinese Academy of SciencesBeijing 100093China

Most mechanistic details of chronologically ordered regulation of leaf senescence are *** networks centered on AtWRKY53 are crucial for orchestrating and integrating various senescence-related ***,AtWRKY53binds to its own promoter and represses transcription of AtWRKY53,but the biological significance and mechanism underlying this selfrepression remain *** this study,we identified the VQ motif-containing protein AtVQ25as a cooperator of *** expression level of AtVQ25 peaked at mature stage and was specifically repressed after the onset of leaf ***25-overexpressing plants and atvq25 mutants displayed precocious and delayed leaf senescence,***,we identified AtWRKY53 as an interacting partner of *** determined that interaction between AtVQ25 and AtWRKY53 prevented AtWRKY53from binding to W-box elements on the AtWRKY53promoter and thus counteracted the selfrepression of *** addition,our RNA-sequencing data revealed that the AtVQ25-AtWRKY53 module is related to the salicylic acid(SA)*** leaf senescence and SA-induced leaf senescence in AtVQ25-overexpressing lines were inhibited by an SA pathway mutant,atsid2,and Nah G transgenic plants;AtVQ25-overexpressing/atwrky53 plants were also insensitive to SA-induced leaf ***,we demonstrated that AtVQ25 directly attenuates the self-repression of AtWRKY53 during the onset of leaf senescence,which is substantially helpful for understanding the timing of leaf senescence onset modulated by AtWRKY53.

关键词： AtWRKY53 leaf senescence salicylic acid transcriptional regulation VQ motif-containing proteins

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产菊粉酶菌株的筛选及其产酶条件的优化

南京农业大学学报 2007年第2期30卷 73-77页

作者：王琳刘兆普赵耕毛隆小华刘冲南京农业大学资源与环境科学学院江苏南京210095

从山东滨海盐碱地种植菊芋的根际土壤中分离得到能产菊粉酶的菌株46株,经过进一步的摇瓶复筛,获得了产菊粉酶能力较高的1株菌株,经初步鉴定为青霉Penicilliumsp.B01。在对其发酵条件优化后,确立了Penicilliumsp.B01最适宜的产酶条件为(g... 详细信息

从山东滨海盐碱地种植菊芋的根际土壤中分离得到能产菊粉酶的菌株46株,经过进一步的摇瓶复筛,获得了产菊粉酶能力较高的1株菌株,经初步鉴定为青霉Penicilliumsp.B01。在对其发酵条件优化后,确立了Penicilliumsp.B01最适宜的产酶条件为(g.L-1):菊芋提取液(EJA)120、酵母膏(YE)25、NH4H2PO42.5、NaCl 5.0、FeSO4.7H2O0.1、ZnSO4.7H2O 0.1,初始pH7,接种2.5×106mL-1的孢子悬浮液3.75%,250 mL的三角瓶中装40 mL的发酵培养基,在30℃、170 ***-1下发酵3 d后菊粉酶活性最高可达25.78 ***-1。此酶主要为外切酶。

关键词：筛选青霉菊粉酶产酶条件优化

A Chinese Multi-Specialty Delphi Consensus to Optimize RAASi Usage and Hyperkalaemia Management in Patients with Chronic Kidney Disease and Heart Failure

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Chinese Medical Sciences Journal 2024年第2期39卷 79-90页

作者： Ming-Hui zhao Wei Chen Hong Cheng Bi-Cheng Liu Zhi-Guo mao Zhuang Tian Gang Xu Jing-Min Zhou Renal Division Peking University First HospitalBeijing 100034China Department of Nephrology The First Affiliated HospitalSun Yat-Sen UniversityGuangzhou 510080China Department of Nephrology Capital Medical UniversityBeijing 100029China Department of Nephrology Southeast University Zhongda HospitalNanjing 210009China Department of Nephrology Shanghai Changzheng HospitalShanghai 200003China Department of Cardiology Peking Union Medical College HospitalBeijing 100730China Renal Division Tongji Medical College of Huazhong University of Science and TechnologyWuhan 430030China Department of Cardiology Zhongshan HospitalFudan UniversityShanghai 200032China

Objective Variations are present in common clinical practices regarding best practice in managing hyperkalaemia(HK),there is therefore a need to establish a multi-specialty approach to optimal renin angiotension-aldosterone system inhibitors(RAASi)usage and HK management in patients with chronic kidney disease(CKD)&heart failure(HF).This study aimed to establish a multi-speciality approach to the optimal use of RAASi and the management of HK in patients with CKD and *** A steering expert group of cardiology and nephrology experts across China were convened to discuss challenges to HK management through a nominal group *** group then created a list of 41 statements for a consensus questionnaire,which was distributed for a further survey in extended panel group of cardiologists and nephrologists across *** was assessed using a modified Delphi technique,with agreement defined as"strong"(≥75%and<90%)and"very strong"(≥90%).The steering group,data collection,and analysis were aided by an independent *** A total of 150 responses from 21 provinces across China were recruited in the *** were comprised of an even split(n=75,50%)between cardiologists and *** 41 statements achieved the 75%consensus agreement threshold,of which 27 statements attained very strong consensus(≥90%agreement)and 14 attained strong consensus(agreement between 75%and 90%).Conclusion Based on the agreement levels from respondents,the steering group agreed a set of recommendations intended to improve patient outcomes in the use of RAASi therapy and HK management in China.

关键词： Sardiorenal syndrome chronic kidney failure heart failure hyperkalemia multidisciplinary communication RAASi

Iron oxide/CNT-based artificial nacre for electromagnetic interference shielding

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Nano Research 2024年第7期17卷 6560-6566页

作者： Cheng-Xin Yu Yu-Feng Meng Bo Yang Jun Pang Xiang-Sen Meng Zi-Ye zhao Qing-Yue Wang Li-Bo mao Zhi-Kun Wu Shu-Hong Yu Institute of Physical Science and Information Technology Anhui UniversityHefei 230601China Key Laboratory of Materials Physics Anhui Key Laboratory of Nanomaterials and NanotechnologyCAS Center for Excellence in NanoscienceInstitute of Solid State PhysicsHFIPSChinese Academy of SciencesHefei 230031China Department of Chemistry New Cornerstone Science LaboratoryInstitute of Biomimetic Materials&ChemistryAnhui Engineering Laboratory of Biomimetic MaterialsDivision of Nanomaterials&ChemistryHefei National Research Center for Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefei 230026China

Biological structural materials,despite consisting of limited kinds of compounds,display multifunctionalities due to their complex hierarchical *** some biomimetic strategies have been applied in artificial materials to enhance their mechanical stability,the simultaneous optimization of other functions along with the mechanical properties via biomimetic designs has not been thoroughly ***,iron oxide/carbon nanotube(CNT)-based artificial nacre with both improved mechanical and electromagnetic interference(EMI)shielding performance is fabricated via the mineralization of Fe_(3)O_(4)onto a CNTincorporated *** micro-and nano-structures of the artificial nacre are similar to those of natural nacre,which in turn improves its mechanical *** alternating electromagnetic wave-reflective CNT layers and the wave-absorptive iron oxide layers can improve the multiple reflections of the waves on the surfaces of the reflection layers,which then allows sufficient interactions between the waves and the absorption ***,compared with the reflection-dependent EMI-shielding of the non-structured material,the artificial nacre exhibits strong absorption-dependent shielding behavior even with a very low content of wave-absorptive *** to the high mechanical stability,the shielding effectiveness of the artificial nacre that deeply cut by a blade is still maintained at approximately 70%−96%depending on the incident wave *** present work provides a new way for designing structural materials with concurrently enhanced mechanical and functional properties,and a path to combine structural design and intrinsic properties of specific materials via a biomimetic strategy.

关键词： artificial nacre electromagnetic interference shielding biomineralization biomimetic material hierarchical structure

Chromosome-level genome and population genomics of the intermediate horseshoe bat(Rhinolophus affinis)reveal the molecular basis of virus tolerance in Rhinolophus and echolocation call frequency variation

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Zoological Research 2024年第5期45卷 1147-1160页

作者： Le zhao Jiaqing Yuan Guiqiang Wang Haohao Jing Chen Huang Lulu Xu Xiao Xu Ting Sun Wu Chen Xiuguang mao Gang Li College of Life Sciences Shaanxi Normal UniversityXi’anShaanxi 710119China QinLing-Bashan Mountains Bioresources Comprehensive Development C.I.C. School of Bioscience and EngineeringShaanxi University of TechnologyHanzhongShaanxi 723001China Guangzhou Zoo GuangzhouGuangdong 510070China School of Ecological and Environmental Sciences East China Normal UniversityShanghai 200241China

Horseshoe bats(genus Rhinolophus,family Rhinolophidae)represent an important group within chiropteran phylogeny due to their distinctive traits,including constant high-frequency echolocation,rapid karyotype evolution,and unique immune *** in evolutionary biology,supported by high-quality reference genomes and comprehensive whole-genome data,have significantly enhanced our understanding of species origins,speciation mechanisms,adaptive evolutionary processes,and phenotypic ***,genomic research and understanding of the evolutionary patterns of Rhinolophus are severely constrained by limited data,with only a single published genome of *** currently *** this study,we constructed a high-quality chromosome-level reference genome for the intermediate horseshoe bat(***).Comparative genomic analyses revealed potential genetic characteristics associated with virus tolerance in ***,we observed expansions in several immune-related gene families and identified various genes functionally associated with the SARS-CoV-2 signaling pathway,DNA repair,and apoptosis,which displayed signs of rapid *** addition,we observed an expansion of the major histocompatibility complex class II(MHC-II)region and a higher copy number of the HLA-DQB2 gene in horseshoe bats compared to other chiropteran *** on whole-genome resequencing and population genomic analyses,we identified multiple candidate loci(e.g.,GLI3)associated with variations in echolocation call frequency across *** *** research not only expands our understanding of the genetic characteristics of the Rhinolophus genus but also establishes a valuable foundation for future research.

关键词： Reference-quality genome Comparative genomics Population genomics Positive selection Bats

海水养殖废水灌溉条件下山东滨海盐土溶质迁移及其平衡研究

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水土保持学报 2005年第3期19卷 80-82,109页

作者：赵耕毛刘兆普陈铭达寇伟锋南京农业大学资源与环境科学学院南京210095

在半干旱的山东莱州地区,利用小型蒸渗装置研究海水养殖废水灌溉下滨海盐土溶质迁移特征以及溶质平衡。结果表明:在耐盐作物-菊芋的水分临界期(6月初)和水分最大效率期(9月初)海水养殖废水灌溉两次,根区土壤中的盐分对于不同淋洗分数的... 详细信息

在半干旱的山东莱州地区,利用小型蒸渗装置研究海水养殖废水灌溉下滨海盐土溶质迁移特征以及溶质平衡。结果表明:在耐盐作物-菊芋的水分临界期(6月初)和水分最大效率期(9月初)海水养殖废水灌溉两次,根区土壤中的盐分对于不同淋洗分数的处理分别增加了89 78,92 35,96 10和102 85g,部分盐分被淋出土体,淋洗效率分别达到17 3%,24 4%,31 1%和36 8%。海水养殖废水灌溉后,土壤中的养分如硝态氮、氨态氮和活性磷酸盐均有所增加,其迁移能力分别为硝态氮>活性磷酸盐>氨态氮,说明硝态氮极易淋失。在选择较小淋洗分数(0 1和0 2)的情况下,大部分的氨态氮和活性磷酸盐被保留在土壤中成为作物的有效养分。此外,大强度的降雨对盐分的淋洗也起到关键性的作用,但也会导致养分的淋失。因此,适时适宜矿化度的海水养殖废水结合适当的淋洗分数补充灌溉耐盐植物,有效地增加了土壤养分,而盐分不会过量累积。

关键词：海水养殖废水滨海盐土淋洗分数溶质平衡

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半干旱地区海水养殖废水灌溉菊芋效应初探

干旱地区农业研究 2005年第5期23卷 159-163页

作者：赵耕毛刘兆普陈铭达寇伟锋南京农业大学资源与环境科学学院江苏南京210095

2004年4～10月,在半干旱的莱州地区布置微区试验,以研究海水养殖废水与微咸水混合灌溉菊芋的作物效应.结果表明:水分临界期淡水灌溉明显促进菊芋茎杆生长,而其它处理茎杆生长受到了不同程度抑制.1∶3,1∶4处理菊芋茎杆的横向生长最快,... 详细信息

2004年4～10月,在半干旱的莱州地区布置微区试验,以研究海水养殖废水与微咸水混合灌溉菊芋的作物效应.结果表明:水分临界期淡水灌溉明显促进菊芋茎杆生长,而其它处理茎杆生长受到了不同程度抑制.1∶3,1∶4处理菊芋茎杆的横向生长最快,茎粗分别达到3.0 cm和2.97 cm;其次为CK1、CK2和1∶2处理,1∶1处理菊芋茎粗最小.未灌溉处理地上部的生物量最高,达到2.92 kg/株.CK2次之,而各海水养殖废水与微咸水混合灌溉处理,地上部生物量有所下降,其中1∶3、1∶4处理下降21.6%和17.5%;1∶1和1∶2处理则分别下降26.4%和29.1%.地下部生物量最高,CK2次之,但与其它处理(除1∶4处理)无明显差异;1∶1处理地下部生物量最低.水分胁迫能够促进菊芋茎、叶、根系生长,但限制了块茎的形成和干物质的积累,而低矿化度的海水养殖废水与微咸水混合灌溉促进了菊芋块茎的正常膨大以及干物质的累积,从而取得最高产量.

关键词：废水灌溉菊芋株高茎粗生物量

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去花对菊芋干物质和糖分积累与分配的影响

草业学报 2014年第1期23卷 149-157页

作者：李辉许欢欢赵耕毛梁明祥南京农业大学资源与环境科学学院江苏南京210095 江苏省海洋生物学重点实验室江苏南京210095

本研究以菊芋品种青芋二号为试材,在温室进行全生育期栽培试验,研究了青芋二号干物质和糖分积累分配以及去花处理(摘掉花朵)对青芋二号块茎干物质和糖分含量的影响。结果表明,叶片中主要糖分是还原糖,块茎、茎中主要是非还原糖。青芋二... 详细信息

本研究以菊芋品种青芋二号为试材,在温室进行全生育期栽培试验,研究了青芋二号干物质和糖分积累分配以及去花处理(摘掉花朵)对青芋二号块茎干物质和糖分含量的影响。结果表明,叶片中主要糖分是还原糖,块茎、茎中主要是非还原糖。青芋二号在开花期时总生物量达到最大值,之后地上部生物量减少,地下部由于此时块茎正处于膨大生长阶段,生物量继续增加。去花处理对青芋二号的块茎生物量有着明显的影响,具体表现为块茎数量增多了39.67%,块茎个头变大了59.46%,块茎干重比未去花的增加22.53%。去花植株相同干重下的块茎组织中的总糖含量与未去花植株相比显著降低但还原糖含量没有差异。通过HPLC-ELSD(高效液相色谱-蒸发光检测仪)检测发现去花处理对糖的成分与含量也有影响,蔗果五糖含量增加了32.47%,更高聚合度的果聚糖有增加的趋势,但是其他糖的含量与对照相比差异不大。

关键词：菊芋去花生物量果聚糖 HPLC