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TBC1D1 is an energy-responsive polarization regulator of macrophages via governing ROS production in obesity

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Science China(Life Sciences) 2024年第9期67卷 1899-1914页

作者： Qi Wang Ping Rong Wen zhang.xinyu Yang Liang Chen Ye Cao Minjun Liu Weikuan Feng Qian Ouyang Qiaoli Chen Hailong Li Hui Liang Fanguo Meng Hong-Yu Wang Shuai Chen State Key Laboratory of Pharmaceutical Biotechnology Department of CardiologyNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolModel Animal Research CenterSchool of MedicineNanjing UniversityNanjing 210061China MOE Key Laboratory of Model Animal for Disease Study Department of EndocrinologyNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolModel Animal Research CenterSchool of MedicineNanjing UniversityNanjing 210061China College of Life Science Anhui Medical UniversityHefei 230032China Redox Medical Center for Public Health Medical College of Soochow UniversitySuzhou 215123China Department of General Surgery First Affiliated HospitalNanjing Medical UniversityNanjing 210029China

Energy status is linked to the production of reactive oxygen species(ROS)in macrophages,which is elevated in ***,it is unclear how ROS production is upregulated in macrophages in response to energy overload for mediating the development of ***,we show that the Rab-GTPase activating protein(Rab GAP)TBC1D1,a substrate of the energy sensor AMP-activated protein kinase(AMPK),is a critical regulator of macrophage ROS production and consequent adipose inflammation for obesity ***1D1 deletion decreases,whereas an energy overload-mimetic non-phosphorylatable TBC1D1^(S231A)Amutation increases,ROS production and M1-like polarization in ***,TBC1D1 and its downstream target Rab8a form an energy-responsive complex with NOX2 for ROS *** of TBC1D1^(S231A)bone marrow aggravates diet-induced obesity whereas treatment with an ultra-stable Tt SOD for removal of ROS selectively in macrophages alleviates both TBC1D1~(S231A)mutation-and diet-induced *** findings therefore have implications for drug discovery to combat obesity.

关键词： TBC1D1 AMPK Rab8a NOX2 ROS inflammation macrophage obesity

DYNAMICS FOR A CHEMOTAXIS MODEL WITH GENERAL LOGISTIC DAMPING AND SIGNAL DEPENDENT MOTILITY

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Acta Mathematica Scientia 2024年第3期44卷 1046-1063页

作者：涂馨予穆春来邱蜀燕张静 School of Mathematics and Statistics Southwest UniversityChongqing400715China Department of Applied Mathematics The Hong Kong Polytechnic UniversityHung HomHong KongChina College of Mathematics and Statistics Chongqing UniversityChongqing401331China School of Sciences Southwest Petroleum UniversityChengdu610500China

In this paper,we consider the fully parabolic Chemotaxis system with the general logistic source{ut=Δ(γ(v)u)+λu-μu^(k),x∈Ω,t>0,vt=△v+wz,x∈Ω,t>0,wt=-wz,x∈Ω,t>0,zt=△z-z+u,x∈Ω,t>0 whereΩ⊂ℝn(n≥1)is a smooth and bounded domain,λ≥0,μ≥0,κ>1,and the motility function satisfies thatγ(v)∈C3([0,∞)),γ(v)>0,γ′(v)≤0 for all v≥*** the Neumann boundary condition,we obtain the global boundedness of solutions if one of the following conditions holds:(i)λ=μ=0,1≤nλ3;(ii)λ>0,μ>0,combined withκ>1,1≤n≤3 or k>n+2/4,,n>***,we prove that the solution (u, v, w, z) exponentially converges to the constant steady state ((λ/μ)1/k-1,∫Ωv0dx+∫Ωw0dx/|Ω|,0,(λ/μ)1/k-1).

关键词： chemotaxis signal-dependent motility logistic source boundedness asymptotic behavior

Two nanobody‑based immunoassays to differentiate antibodies against genotype 1 and 2 porcine reproductive and respiratory syndrome virus

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Animal Diseases 2024年第2期4卷 98-114页

作者： Xu Chen Yueting Chang Lu zhang.xinyu Zhao Zhihan Li Zhijie zhang Pinpin Ji Qingyuan Liu Jiakai Zhao Jiahong Zhu Baoyuan Liu Xinjie Wang Yani Sun Qin Zhao Department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F UniversityYanglingShaanxi 712100China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518100China

Porcine reproductive and respiratory syndrome virus(PRRSV)infection causes significant economic loss to the global pig *** 1 and 2 PRRSV(PRRSV-1 and-2)infections have been reported in China,Europe and *** accurate prevention,nanobodies were first used as diagnostic reagents for PRRSV *** this study three nanobodies targeting both PRRSV-1 and-2,two targeting PRRSV-1 and three targeting PRRSV-2,were screened and *** develop two competitive ELISAs(cELISAs),the g1-2-PRRSV-Nb3-HRP nanobody was chosen for the g1-2-cELISA,to detect common antibodies against PRRSV-1 and-2,and the g1-PRRSV-Nb136-HRP nanobody was chosen for the g1-cELISA,to detect anti-PRRSV-1 *** two cELISAs were developed using PRRSV-1-N protein as coating antigen,and the amounts for both were 100 ng/*** optimized dilution of testing pig sera was 1:20,the optimized reaction times were 30 min,and the colorimetric reaction times were 15 ***,the cut-off values of the g1-2-cELISA and g1-cELISA were 26.6%and 35.6%,*** of them have high sensitivity,strong specificity,good repeatability,and *** addition,for the 1534 clinical pig sera,an agreement rate of 99.02%(Kappa values=0.97)was determined between the g1-2-cELISA and the commercial IDEXX ELISA *** the g1-cELSIA,it can specifically detect anti-PRRSV-1 antibodies in the clinical pig ***,combining two nanobody-based cELISAs can differentially detect antibodies against PRRSV-1 and-2.

关键词： PRRSV Competitive ELISA Nanobody Antigen epitope

叙事、视听、认知三重建构:近年中国犯罪片电影视点创作研究

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当代电影 2024年第9期 101-107页

作者：张馨宇中国传媒大学戏剧影视学院

纵观近几年国产犯罪片,凭借其引人入胜的解谜元素,引发了观众对该类型影片的偏好。该类型特点与电影视点的使用密不可分,多视点在构建非线性叙事中的核心作用,为犯罪片叙事手法提供了更多可能性。本文从电影视点的界定和划分入手,将使... 详细信息

纵观近几年国产犯罪片,凭借其引人入胜的解谜元素,引发了观众对该类型影片的偏好。该类型特点与电影视点的使用密不可分,多视点在构建非线性叙事中的核心作用,为犯罪片叙事手法提供了更多可能性。本文从电影视点的界定和划分入手,将使用策略分为三个层面进行阐述,分别为信息多少、信息真假、人物多少,其中涉及内外零聚焦、多种内聚焦手法、多视点使用、可靠和不可靠叙述者、叙事与视听视点等方面的分析,最后针对由于视点界限交叉造成的叙事视点和视听视点的统一与背离这一问题进行了阐述。通过五部分的分析希望能够找到国产犯罪片近年快速发展的原因以及未来发展的趋势的启示。

关键词：犯罪片叙事视点视听视点多视点不可靠叙述者

Pressure generation under deformation in a large-volume press

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Chinese Physics B 2024年第9期33卷 622-626页

作者： Saisai Wang xinyu Zhao Kuo Hu Bingtao Feng Xuyuan Hou Yiming zhang Shucheng Liu Yuchen Shang Zhaodong Liu Mingguang Yao Bingbing Liu State Key Laboratory of Superhard Materials Synergetic Extreme Condition User FacilityCollege of PhysicsJilin UniversityChangchun 130012China College of Earth Sciences Jilin UniversityChangchun 130012China

Deformation can change the transition pathway of materials under high pressure,thus significantly affects physical and chemical properties of ***,accurate pressure calibration under deformation is challenging and thereby causes relatively large pressure uncertainties in deformation experiments,resulting in the synthesis of complex multiphase ***,pressure generations of three types of deformation assemblies were well calibrated in a Walker-type largevolume press(LVP)by electrical resistance measurements combined with finite element simulations(FESs).Hard Al_(2)O_(3) or diamond pistons in shear and uniaxial deformation assemblies significantly increase the efficiency of pressure generation compared with the conventional quasi-hydrostatic *** uniaxial deformation assembly using flat diamond pistons possesses the highest efficiency in these deformation *** finding is further confirmed by stress distribution analysis based on *** this deformation assembly,we found shear can effectively promote the transformation of C60 into diamond under high pressure and realized the synthesis of phase-pure diamond at relatively moderate pressure and temperature *** present developed techniques will help improve pressure efficiencies in LVP and explore the new physical and chemical properties of materials under deformation in both science and technology.

关键词： shear/uniaxial deformation pressure calibration finite element simulations large- volume press high pressure

Piezo1 channel exaggerates ferroptosis of nucleus pulposus cells by mediating mechanical stress-induced iron influx

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Bone Research 2024年第2期12卷 334-348页

作者： Ziqian Xiang Pengfei zhang Chunwang Jia Rongkun Xu Dingren Cao Zhaoning Xu Tingting Lu Jingwei Liu Xiaoxiong Wang Cheng Qiu Wenyang Fu Weiwei Li Lei Cheng Qiang Yang Shiqing Feng Lianlei Wang Yunpeng Zhao xinyu Liu Department of Orthopaedics Qilu Hospital of Shandong UniversityJinan 250012China University of Health and Rehabilitation Sciences Qingdao 226000China Xiangya School of Medicine Central South UniversityChangsha 410013China School of Nursing and Rehabilitation Shandong UniversityJinan 250012China Department of Pediatrics Cangzhou Central HospitalCangzhou 061011China Department of Pediatric Surgery Qilu Hospital of Shandong UniversityJinan 250012China Department of Pathology Qilu Hospital of Shandong UniversityJinan 250012China Department of Spine Surgery Tianjin HospitalTianjin UniversityTianjin 30021China The Second Hospital of Shandong University Cheeloo College of MedicineShandong UniversityJinan 250012China

To date,several molecules have been found to facilitate iron influx,while the types of iron influx channels remain to be ***,Piezo1 channel was identified as a key iron transporter in response to mechanical ***1-mediated iron overload disturbed iron metabolism and exaggerated ferroptosis in nucleus pulposus cells(NPCs).Importantly,Piezo1-induced iron influx was independent of the transferrin receptor(TFRC),a well-recognized iron ***,pharmacological inactivation of Piezo1 profoundly reduced iron accumulation,alleviated mitochondrial ROS,and suppressed ferroptotic alterations in stimulation of mechanical ***,conditional knockout of Piezo1(Col2a1-CreERT Piezo1^(flox/flox))attenuated the mechanical injury-induced intervertebral disc degeneration(IVDD).Notably,the protective effect of Piezo1 deficiency in IVDD was dampened in Piezo1/Gpx4 conditional double knockout(cDKO)mice(Col2a1-CreERT Piezo1^(flox/flox)/Gpx4^(flox/flox)).These findings suggest that Piezo1 is a potential determinant of iron influx,indicating that the Piezo1-iron-ferroptosis axis might shed light on the treatment of mechanical stress-induced diseases.

关键词： stress overload channel

Multi-genome evolutionary study of the ABC1 gene family and identification of the pleiotropic effects of OsABC1-13 in rice development

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The Crop Journal 2024年第4期12卷 1022-1030页

作者： Fuying Ma Mingyu Liu Peiwen Yan Shicong He Jian Hu Xinwei zhang Fuan Niu Jinhao Cui xinyu Yuan Xiaoyun Xin Liming Cao Jinshui Yang Ying Wang Xiaojin Luo State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology(Ministry of Education) School of Life SciencesFudan UniversityShanghai 200433China Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Department of Ecology and Evolutionary BiologySchool of Life SciencesFudan UniversityShanghai 200433China Institute of Crop Breeding and Cultivation Shanghai Academy of Agricultural SciencesShanghai 200241China Ministry of Education Key Laboratory of Crop PhysiologyEcology and Genetic Breeding College of AgronomyJiangxi Agricultural UniversityNanchang 330045JiangxiChina

In four rice genomes,85 ABC1-family genes were identified by comparative genomics,evolution,genetics,and ***,OsABC1-13,was shown by knockdown and knockout experiments to affect plant height,grain size,and photosynthet... 详细信息

关键词： Multi-genome analysis Activity of bc1 complex gene Photosynthesis Biomass Osabc1-13 Haplotype

The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson's disease

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Neural Regeneration Research 2025年第8期20卷 2361-2372页

作者： Shengyang Zhou Ting Li Wei zhang Jian Wu Hui Hong Wei Quan xinyu Qiao Chun Cui Chenmeng Qiao Weijiang Zhao Yanqin Shen Laboratory of Neurodegenerative and Neuroinjury Diseases Wuxi Medicine SchoolJiangnan UniversityWuxiJiangsu ProvinceChina

Interferon regulatory factor 7 plays a crucial role in the innate immune ***,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains *** we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial *** the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 *** addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and *** together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.

关键词： cyclic guanosine monophosphate adenosine monophosphate synthase H151 interferon regulatory factor 7 M1 phenotype neurodegenerative disease neuroinflammation Parkinson’s disease RU521 STING type I interferon

Buried interface bridging for inverted cesium-formamidinium triiodide perovskite solar cells with long operational stability

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Science China Chemistry 2024年

作者： Chenhui zhang Chunjun Liang Fulin Sun Ting Zhu Xinghai Huang Yuzhu Guo xinyu Guo Kunyang Ge Dan Li Fangtian You Zhiqun He Key Laboratory of Luminescence and Optical Information Ministry of Education Institute of Optoelectronic TechnologyBeijing Jiaotong University Department of Physics School of Physical Science and Engineering Beijing Jiaotong University

In the field of perovskite solar cells(PSCs), the research on defects in the buried interface has been relatively limited due to its non-exposure; however, this interface significantly impacts the performance enhancement of inverted PSCs. This study employs phenylethylammonium chloride(PEACl) molecules as a buffer layer to modify the buried interface of p-i-n structured PSCs,aiming to enhance the uniformity of self-assembled monolayers(SAMs) and facilitate the uniform nucleation and growth of perovskite films on the substrate. Furthermore, the introduction of the PEACl buffer layer effectively passivates defects at the bottom of the perovskite layer and notably enhances the crystal quality of the perovskite film by mitigating residual stress,thereby reducing nonradiative recombination loss. Following these optimizations, the MA-free PSCs treated with PEACl achieve a power conversion efficiency(PCE) of 24.11%, with significant improvements in storage, thermal stability, and operational stability. Particularly noteworthy is the device's performance in an unencapsulated state, whereas after 1,500 hours of continuous light operation stability testing, it retains 97% of its original efficiency. This study not only enriches the systematic understanding of the characteristics of the buried interface in PSCs but also contributes significantly to advancing the commercial production of perovskite photovoltaic technology.

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