检索结果-南通市图书馆

Yeast-Derived β-1,3-Glucan Substrate Significantly Increased the Diversity of Methanogens During In vitro Fermentation of Porcine Colonic Digesta

引用

Journal of Integrative Agriculture 2013年第12期12卷 2229-2234页

作者： LUO Yu-heng LI Hua LUO Jun-qiu zhang ke-ying key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province and China Ministry of Education/Institute of Animal Nutrition Sichuan Agricultural University

The β-1,3-glucan from yeast has been extensively examined for its immuno-enhancing effects in ***,investigation on the relationship among β-glucan,gut microbiota and immune-modulating effects remains limited particularly in *** the critical roles of gut methanogens in the microbial fermentation,energy metabolism and disease resistance,we investigated the phylogenetic diversity of methanogens from fermented cultures of porcine colonic digesta with（G） or without（N） yeast β-glucan based on sequences of the archaeal 16S rRNA gene.A total of 145 sequences in the G library were assigned into 8 operational taxonomic units（OTUs） with the majority of sequences（114/145） related to strains Methanobrevibacter millerae or Methanobrevibacter gottschalkii with high identities ranging from 97.9 to 98.6%,followed by 23 sequences to Methanobrevibacter ruminantium,2 sequences to Methanobrevibacter smithii and one sequence to Methanobrevibacter *** 142 sequences in the N library were assigned to 2 OTUs with most sequences（127/142） related to strains *** or *** with sequence identities ranging from 97.9 to 98.5%,and 15 sequences related to *** with 97.9% *** diversity index showed that the G library exhibited significantly higher archaeal diversity（P0.05） and Libshuff analysis indicated the differences in the community structure between the two libraries were significant（P0.0001）.In conclusion,the current study provides evidence that addition of yeast β-glucan significantly increased the diversity of methanogens in in vitro fermented porcine colonic digesta.

关键词： β-glucan methanogen diversity pig colonic digesta in vitro

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Impacts of the Boreal Summer Intraseasonal Oscillation on the Warm-Season Rainfall over Hainan Island

引用

Journal of Tropical Meteorology 2022年第4期28卷 457-472页

作者： MA Wen-lan ZHU Lei FENG Xiao zhang ke-ying key Laboratory of Meteorological Disaster(KLME) Ministry of Education and Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters(CIC-FEMD)Nanjing University of Information Science and TechnologyNanjing 210044 China key Laboratory of South China Sea Meteorological Disaster Prevention and Mitigation of Hainan Province Haikou 570203 China College of Oceanic and Atmospheric Sciences Ocean University of ChinaQingdaoShandong 266100 China

This study investigates the roles of the boreal summer intraseasonal oscillation(BSISO)in the diurnal rainfall cycle over Hainan Island during the warm season(April-September)using 20-year satellite-based precipitation,ERA5 and the outgoing longwave radiation data with the phase composite analysis *** show that the spatial distributions of the hourly rainfall anomaly significantly change under the BSISO phases 1-8 while no clear variations are found on the daily and anomaly daily area-averaged rainfall over the *** the BSISO phase 1,the rainfall anomaly distinctly increases in the morning over the southwest and late afternoon over the northeast of the island,while suppressed convection occurs in the early afternoon over the southwest *** this circumstance,strong low-level westerly winds bring abundant moisture into the island,which helps initiate the nocturnal-morning convection over the south coastal area,and drives the convergence region of sea breeze fronts to concentrate into the *** to Phase 1,an almost completely reversed diurnal cycle of rainfall anomaly is found in Phase 5,whereas a positive anomalous rainfall peak is observed in the early afternoon over the center while negative peaks are found in the morning and late afternoon over the southwest and northeast,owing to a strong low-level northeasterly anomaly flow,which causes relatively low moisture and enlarges a sea-breeze convergence area over the *** Phase 8,strongest moisture is found over the island all through the day,which tends to produce highest rainfall in the afternoon with enhanced anomalous *** results further indicate that multiscale interactions between the large-scale circulations and local land-sea breeze circulations play important roles in modulating diurnal precipitation cycles over the tropical island.

关键词： BSISO diurnal rainfall cycle Hainan Island sea breeze large-scale circulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cellular localization and biochemical characterization of a novel calciumdependent protein kinase from tobacco

引用

Cell Research 2005年第8期15卷 604-612页

作者： Yun WANG?, Mei zhang?, ke ke, ying Tang LU* key Lab of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China key Lab of MOE for Plant Developmental Biology College of Life Sciences Wuhan University Wuhan China

By screening tobacco cDNA library with MCK1 as a probe, we isolated a cDNA clone NtCPK5 (accession number AY971376), which encodes a typical calcium-dependent protein kinase. Sequence analyses indicated that NtCPK5 is related to both CPKs and CRKs superfamilies and has all of the three conserved domains of CPKs. The biochemical activity of NtCPK5 was calcium-dependent. NtCPK5 had Vmax and Km of 526 nmol/min/mg and 210 μg/ml respectively with calf thymus histone (fraction III, abbreviated to histone IIIs) as substrate. For substrate syntide-2, NtCPK5 showed a higher Vmax of 2008 nmol/min/mg and a lower Km of 30 μM. The K0.5 of calcium activation was 0.04 μM or 0.06 μM for histone IIIs or syntide-2 respectively. The putative myristoylation and palmitoylation consensus sequence of NtCPK5 suggests that it could be a membrane-anchoring protein. Indeed, our transient expression experiments with wild type and mutant forms of NtCPK5/GFP fusion proteins showed that NtCPK5 was localized to the plasma mem- brane of onion epidermal cells and that the localization required the N-terminal acylation sites of NtCPK5/GFP. Taking together, our data have demonstrated the biochemical characteristics of a novel protein NtCPK5 and its subcellular localization as a membrane-anchoring protein.

关键词： calcium-dependent protein kinase phosphorylation GFP subcellular localization.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Tissue Deposition and Residue Depletion in Broiler Exposed to Melamine-Contaminated Diets

引用

Journal of Integrative Agriculture 2012年第1期11卷 109-115页

作者： DING Xue-mei BAI Shi-ping zhang ke-ying WANG Liang WU Cai-mei CHEN Dai-wen JIA Gang BAI Jie Institute of Animal Nutrition Key Laboratory for Animal Disease-Resistance Nutrition of ChinaMinistry of EducationSichuan Agricultural UniversityYaan 625014P.R.China

To investigate the tissue deposition and elimination of melamine （MEL） in broilers, a total of 1 920 commercial 1-d-old male Cobb broilers were randomly allotted to 6 treatments with diets contaminated by MEL at 0, 2, 10, 30, 50, 100 mg kg-1 of diet for 42 d, and followed MEL withdrawal from diet at 50 or 100 mg kg-1 of diet for 96 h. The MEL was found in residue of plasma, liver, kidney, breast, and leg muscle with the highest level in kidney and in dose response manner （except in kidney）, but no time response （except in plasma）. The MEL residue in organ tissue was below the safe level of 50 μg mL-1 in blood or 50 μg kg-1 in tissues proposed by the US FSIS when MEL in diet was lower （2 mg kg-1）. The MEL in tissues was eliminated by withdrawal MEL from diets. The elimination half-life of MEL was from 3.2 to 6.6 h, and the clearance time, when MEL residue concentration under limit detection, was from 18.0 to 31.4 h.

关键词： melamine broiler deposition elimination

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers

引用

Journal of Integrative Agriculture 2016年第1期15卷 183-190页

作者： ZENG Qiu-feng YANG Xia ZHENG Ping zhang ke-ying LUO Yu-heng DING Xue-mei BAI Shi-ping WANG Jian-ping XUAN Yue SU Zhuo-wei key Laboratory for Animal Disease Resistance Nutrition Ministry of EducationInstitute of Animal Nutrition Sichuan Agricultural University

The objective of this study was to evaluate the effects of low ambient temperature （LAT） and dietary vitamin C （VC） sup- plementation on pulmonary vascular remodeling （PVR） and the relative expression of hypoxia inducible factor-la （HIF-la）, vascular endothelial growth factor （VEGF） and its receptor 2 （VEGFR-2） mRNA of lungs in 21-d-old broilers. 400 1-d-old male Cobb broilers were assigned randomly to 4 treatments as follows for 21 d： 1 ） LAT and a basal diet; 2） LAT and a basal diet supplemented with 1 000 mg kg-1 VC （LAT＋VC）; 3） normal ambient temperature （NAT） and a basal diet; 4） NAT and a basal diet supplemented with 1 000 mg kg-1 VC （NAT＋VC）. Each treatment was composed of 10 replicates of 10 birds per replicate. Samples of lung were collected after the broilers were killed at d 21. LAT increased the ratio of vessel wall area to vessel total area （WA/TA, %） and mean media thickness in pulmonary arterioles （mMTPA, %）（P〈0.05）. Dietary VC supplementation decreased mMTPA （P〈0.05）, but had no effect on the WA/TA. LAT increased （P〈0.05） the relative mRNA expression of HIF-la, VEGF and VEGFR-2, while adding VC to the diet could decrease （P〈0.05） their relative mRNA expression. A significant positive correlation existed between the level of VEGF mRNA expression and the value of WA/WT （P〈0.05） or mMTPA （P〈0.05）. These results suggested LAT resulted in pulmonary vascular remodeling, and the increase of HIF-la, VEGF and VEGFR-2 mRNA expression, and dietary VC supplementation can alleviate pulmonary vascular remodeling in broiler by affecting these gene expression.

关键词： broiler hypoxia inducible factor-la low ambient temperature pulmonary vascular remodeling vitamin C

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of probiotics on growth performance, development of small intestinal tract and microbial populations in broilers

引用

Journal of Agricultural Science and Technology 2009年第4期3卷 24-31,39页

作者： LEI Yan zhang ke-ying DING Xue-mei BAI Shi-ping JUNG Sik Choi Animal Nutrition Institute Sichuan Agricultural University Ya ＇an 625014 China Engineering Research Center of Animal Disease-Resistance Nutrition of China Ministry of Education Sichuan Agricultural University Ya ＇an 625014 China JSC Bio-teek Company Korea 4490205 Korea

The experiment was conducted using a total of 1392 one-day-old, Cobb commercial male broilers to investigate the effects of a commercial probiotic addition in diet on growth, the development of the small intestine and microbial populations of broilers. Broilers were randomly allotted by BW to 1 of 6 replicate cages （29 chicks per cage） for each of 8 treatments in a randomized block design involving one factorial arrangement of treatments. Dietary treatments included the basal diet （including corn, soybean, rapeseed, cottonseed meal） added with 100 mg/kg antibiotic, 0%, 0.05%, 0.1%, 0.15%, 0.2%, 0.25% or 0.3%. The experiment last 42 d and divided into the starter period （1-21 d） and the finisher period （22-42 d）. The results showed that there were significant differences in broiler body weight （BW）（P〈0.001）, average daily feed intake （ADFI）（P=0.033）, relative length of the duodenum （P〈0.001）, jejunum （P=0.008）, ileum （P〈0.001） and the populations of lactobacillus （P〈0.001） and coliform （P〈0.001） in the rectum between the probiotics treatments and the control treatment only in the starter period of the trails. No significant differences were observed in intestinal morphology parameters in two periods of the experiment. Our results indicated that the probiotic was able to improve the performance of broiler chickens, and it was better in the starter period to be used than in the finisher period.

关键词： probiotic broiler chickens performance intestine development microbial population

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

3年规范化培训考核在提高新入职护士职业能力中的作用

引用

中华护理杂志 2016年第7期51卷 836-839页

作者：朱珑张克英山东大学附属省立医院护理部济南市250021

目的通过护士规范化培训的实施,为全面提高护士职业能力和综合素质,建设护士规范化培训体系的相关研究提供依据。方法实验组为120名入职经过岗前培训后即纳入3年规范化培训的护士,设置严格的培训计划、培训内容和考核体系;对照组为120... 详细信息

目的通过护士规范化培训的实施,为全面提高护士职业能力和综合素质,建设护士规范化培训体系的相关研究提供依据。方法实验组为120名入职经过岗前培训后即纳入3年规范化培训的护士,设置严格的培训计划、培训内容和考核体系;对照组为120名入职经过岗前培训后即到科室轮转1年工作的护士。分别对两组护士3年后的临床决策与应急能力、操作技能、沟通能力、人文修养、健康宣教、临床护理评估及书写护理文书能力进行统计学分析。结果实验组护士经过3年规范化培训,在临床决策与应急能力、操作技能、沟通能力、人文修养、健康宣教及临床护理评估能力高于对照组,实验组总分数为(90.68±2.71)分,对照组为(85.50±2.97)分,差异有统计学意义(P<0.05)。结论 3年规范化培训可有效提高护士的职业能力,促进学校教育与临床护理工作有效链接,在科学地开发和利用护理人力资源等方面起到了显著的效果。

关键词：护士在职培训工作能力评定

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

ARCH模型的诊断分析

引用

管理科学学报 2001年第2期4卷 12-18页

作者：柯珂张世英天津大学管理学院天津300072

探讨了 ARCH模型的诊断分析和变结构建模问题 .所提出的分整增广 GARCH- M模型包括了几乎所有现有的 ARCH模型 ,基于分整增广 GARCH- M模型克服了 ARCH模型在模型设定检验 ,长记忆检验和参数估计等方面的障碍 .利用分段建模方法来检测... 详细信息

探讨了 ARCH模型的诊断分析和变结构建模问题 .所提出的分整增广 GARCH- M模型包括了几乎所有现有的 ARCH模型 ,基于分整增广 GARCH- M模型克服了 ARCH模型在模型设定检验 ,长记忆检验和参数估计等方面的障碍 .利用分段建模方法来检测模型结构变化点和分段变化模型的选择 .最后 ,以上海证券综合事业股票指数为数据。

关键词： ARCH模型诊断分析变结构建模分整增广GARCH-M模型金融波动性分段建模变结构点

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ghrelin:一个重要脑肠肽生物学效应的研究进展

引用

中国畜牧杂志 2006年第7期42卷 39-42页

作者：高庆张克英四川农业大学动物营养研究所四川雅安625014

Ghrelin是第一个被发现的生长激素促分泌素受体(GHS-R)的内源性配体。这个脑肠肽在调节生长激素释放、增进食欲、调节能量代谢等方面发挥重要作用,他还具有其他重要的生物学功能。加强Ghrelin在营养生理学上的研究具有重要的理论意义和... 详细信息

Ghrelin是第一个被发现的生长激素促分泌素受体(GHS-R)的内源性配体。这个脑肠肽在调节生长激素释放、增进食欲、调节能量代谢等方面发挥重要作用,他还具有其他重要的生物学功能。加强Ghrelin在营养生理学上的研究具有重要的理论意义和广阔的应用前景。

关键词：畜牧学生长激素促分泌素综述 Ghrelin 能量代谢调节

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

热休克蛋白70诱导剂及其应用

引用

畜牧与兽医 2008年第1期40卷 98-101页

作者：谭权张克英四川农业大学动物营养研究所四川雅安625014

热休克蛋白70(HSP70)是生物体在应激原刺激下合成的一族进化上高度保守的蛋白质。HSP70家族的功能表现在多方面:作为分子伴侣,HSP70在应激状态下帮助需要折叠的蛋白质正确折叠,加快正常蛋白质合成的恢复;在细胞保护方面,HSP70表达的增... 详细信息

热休克蛋白70(HSP70)是生物体在应激原刺激下合成的一族进化上高度保守的蛋白质。HSP70家族的功能表现在多方面:作为分子伴侣,HSP70在应激状态下帮助需要折叠的蛋白质正确折叠,加快正常蛋白质合成的恢复;在细胞保护方面,HSP70表达的增加可以增强机体对应激的抵抗力,缓解细胞所受伤害。本文主要综述HSP70的生物学特性及其细胞保护作用,并讨论了HSP70诱导剂在畜牧业中潜在的应用意义。

关键词：热休克蛋白70(HSP70) 应激分子伴侣细胞保护休克蛋白70诱导剂

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：