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Clinical significance of costimulatory molecule B7-H3 expression on CD3＋T cells in colorectal carcinoma

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Chinese Medical Journal 2013年第16期126卷 3035-3038页

作者： MAO Yong SUN Jing WANG Wei-peng zhang xue-guang HUA Dong The Fourth Hospital of Wuxi The Fourth Hospital Affiliated to Soochow University Wuxi Jiangsu 214062 China Medical College of Soochow University Suzhou Jiangsu 215007 China

Background B7-H3 has been widely studied in the context of tumor progression in recent years,and behaves as a tumor cell marker in a variety of tumors including colorectal *** mechanism of B7-H3 in tumor progression is complicated and not clear *** have revealed that B7 family molecules are expressed on infiltrated lymphocytes as well as tumor cells in tumor microenvironment,which indicates that different expression pattern may lead to different clinical *** The expression of B7-H3 was detected in tissues of 98 colorectal carcinoma patients by using *** the expression of B7-H3 on CD3＋ T lymphocytes isolated from fresh cancer tissues of 12 colorectal carcinoma patients was analyzed by flow cytometry *** relationship between the expression of B7-H3 on CD3＋ T lymphocytes and patients＇ clinical pathological parameters was demonstrated with statistical *** Patients with more CD3＋ T cell infiltration survived much longer than patients with less CD3＋ T cell infiltration （P ＜0.05）; B7-H3 was highly expressed by infiltrating CD3＋ T lymphocytes in colorectal carcinoma *** expression of B7-H3 was found to be significantly related with lymph node metastasis status （P ＜0.05）,but not with the patient＇s gender,age,tumor size,differentiation degree,depth of tumor invasion,Dukes＇ stage,distant metastasis and whether or not mucinous adenocarcinoma was present （P ＞0.05）.Moreover,the survival time of patients with low expression of B7-H3 was obviously longer than those of high B7-H3 expression patients,but the seven-year survival rate showed no difference between the high and low B7-H3 expression patients （P ＞0.05）.Conclusion The negative costimulatory molecule B7-H3 on infiltrating CD3＋ T lymphocytes in colorectal carcinoma bears importance in the clinical pathological progress and prognosis of colorectal carcinoma.

关键词： colorectal carcinoma B7-H3 T lymphocytes tumor microenvironment

Clinical Analysis of a Hypokalemic Salt-losing Tubulopathy Case

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Chinese Medical Journal 2016年第5期129卷 601-603页

作者： Wei Zheng Quan Hong xue-guang zhang Xiao-Dong Geng guang-Yan Cai Xiang-Mei Chen Di Wu Department of Nephrology Chinese People's Liberation Army General Hospital Chinese People's Liberation Army Institute of Nephrology State Key Laboratory of Kidney Diseases National Clinical Research Center for Kidney Diseases Beijing Key Laboratory of Kidney Disease Beijing 100853 China

Hypokalemia is among the most common electrolyte disorders in clinical practice and severe condition is life-threatening and has received extensive attention in clinical practice. Genetic diagnosis remains the gold standard for the diagnosis and identification of hereditary renal tubular diseases. Below is the report of genotyping and diagnosis of a case of secondary Gitelman syndrome （GS） based on next-generation sequencing.

关键词： Diagnosis Gene Screening Hypokalemia

Altered CD28 and CD95 mRNA expression in peripheral blood mononuclear cells from elderly patients with primary non-small cell lung cancer

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Chinese Medical Journal 2010年第1期123卷 51-56页

作者： WANG Ling PAN Xu-dong XIE Yan zhang guang-bo JIANG Min ZHENG Li WANG Jin-hong SHI Jin-fang zhang xue-guang Clinical Immunology Laboratory Special Requirement Ward Dep.artment of Clinical Laboratory

Background The expression of the co-stimulatory molecule CD28 and death receptor CD95 on T cells, which change with age, are considered as important immunological parameters of immunosenescence. It is well established that CD28 and CD95 are associated with tumorgenesis and tumor progression, but the relationship between the age-related changes of these two immunological markers and cancer in the elderly is largely unknown. Methods The levels of CD28 and CD95 mRNA in peripheral blood mononuclear cells （PBMCs） from sixty-three elderly patients （aged 〉60 years） with primary non-small cell lung cancer （NSCLC） were analyzed by real-time fluorescence-based quantitative polymerase chain reaction （FQ-PCR）. In addition, twenty young patients （aged 〈60 years） with NSCLC, thirty elderly healthy donors and thirty young healthy donors were enrolled as controls. Results CD28 mRNA levels were significantly lower and CD95 mRNA levels were significantly higher in elderly patients with NSCLC than in the other groups. Similar results were found in elderly healthy donors comparing with young healthy donors. By Logistic regression analysis an increased risk of NSCLC was markedly associated with aging, down-regulation of CD28 mRNA and up-regulation of CD95 mRNA, and CD28 mRNA had an obvious negative correlation with the CD95 mRNA. In addition, the mRNA levels of CD28 and CD95 in the peripheral blood of the elderly patients was closely associated with the tumor node metastasis （TNM） stages, grade of cell differentiation and lymph node metastasis status, but not related to pathological types. Conclusions The results suggest a close relationship between T cell senescence and NSCLC tumour progress in the elderly, and that up-regulation of CD28 mRNA or down-regulation of CD95 mRNA in peripheral blood T cells may play an important role in inhibiting oncogenesis and development of primary NSCLC in the elderly.

关键词： aged non-small cell lung carcinoma CD28 CD95 immunosenescence

SIX NEW SPECIES OF TINOCALLIS MATSUMURA AND A NEW SPECIES OF SINOTHERIOAPHIS zhang, GEN. NOV. FROM CHINA (APHIDOIDEA: CALLAPHIDIDAE)

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动物学研究 1980年第4期 433-442页

作者： zhang guang-xue ZHONG TIE-SEN(Institute of Zoology,Academia Sinica)

This paper deals with 12 species of Callaphididae from China. Among them 7 species and a genus are described as new to science. All the types are deposited in the Institute of Zoology, Academia Sinica. Tinocallis hemipteleae zhang, sp. nov. (figs. 1—12) Allied to T. takachihoensis Higuchi, 1972, but forewing with pigment bordering the veins, with spots at apices of veins, and with apical 1/5 of Rs indistinct; 1st segment of all tarsi with 7 setae; 3rd antennal segment with 14—18 transversely elongate sensoria.

关键词：触角节新属新种彩斑 APHIDOIDEA CALLAPHIDIDAE FROM CHINA NOV SIX NEW SPECIES OF TINOCALLIS MATSUMURA AND A NEW SPECIES OF SINOTHERIOAPHIS zhang GEN

Expression of tissue inhibitor of matrix metalloproteinase-1 in aging of transgenic mouse liver

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Chinese Medical Journal 2006年第6期119卷 504-509页

作者： zhang Yu-mei CHEN Xiang-mei WU Di zhang xue-guang LU Yang SHI Suo-zhu YIN Zhong Department of Nephrology General Hospital of PLA Beijing100853 China Department of Nephrology Beijing Military Command GeneralHospital Beijing 100700 China

Background Tissue inhibitor of matrix metaUoproteinase-1 （TIMP-1） is related to the aging of many organs, but few data are available on the change of TIMP-1 in liver aging. The purpose of this study was to investigate the expression and role of TIMP-1, matrix metalloproteinase-2 （MMP-2） and MMP-9 in the process of natural aging in the livers of normal and transgenic mice, and to detect the effects of TIMP-1 on oxidative level and anti-oxidative ability of the livers of transgenic young mice. Methods Normal and transgenic mice were divided into 3 groups according to their age： 3-month-old group （n=5）, 12-month-old group （n=5） and 24-month-old group （n=5）. Histopathological changes of the liver were observed after HE and Masson staining. The messenger RNA （mRNA） levels of TIMP-1, MMP-2 and MMP-9 were determined by semi-quantitative reverse transcriptional polymerase chain reaction; protein expression was measured by Western blot in the livers of normal and transgenic mice of various ages. Changes in levels of superoxide dismutase （SOD）, monoamine oxidase （MAO）, malondialdehyde （MDA） as well as oxidative and anti-oxidative ability were measured. Results Histologically, more fatty degeneration and collagen deposition were found in the aging livers of transgenic mice than in those of the normal mice as their age of months increased. The mRNA and protein expressions of TIMP-1 were significantly high in the oldest animals. The histopathological changes, mRNA and protein expressions of TIMP-1 increased significantly in the liver of transgenic mice as compared with normal mice. The expression of MMP-2 and MMP-9 showed a minor change in the process of aging. Liver change and collagen deposition were not observed in young mice, but the activity of SOD decreased （P〈0.05）, and the activity of MAO （P〈0.01） and the content of MDA increased in the liver of transgenic mice （P〈0.01）. Conclusions The expression of TIMP-1 is significantly high in the liver of transgenic mouse in

关键词： aging tissue inhbitor of matrix metallporoteinase-1 transgenic mice fiver oxidative

The expression and clinical significance of B7-H4 and PKC-δ in Colorectal cancer

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The expression and clinical significance of B7-H4 and PKC-δ...

第十四届全国肿瘤生物治疗大会

作者： Zhou Bin Wang Liang zhang xue guang Institute of Clinical Immunology of Jiangsu Province The First Affiliatied Hospital of Su Zhou Univercity

B7-H4 was over expressed in many cancers and was associated with clinical progression. Although B7-H4 are most discussed, the regulation mechnisms of B7-H4 during tumor cells are unknown. Protein kinase C(PKC), a prototypical class of serine/threonine kinases, exemplifies specific signaling molecules that link multiple cellular processes to cancer. In the last years we have witnessed major advances in our understanding of the roles of PKCs in colorectal cancer development and progression. Objective: To investigate the expression and correlation of B7-H4 and PKC-δ in CRC tissues and analyze the correlation to clinic pathological parameters. Methods: we analyzed the expression of B7-H4 and PKC-δ in 87 colorectal tumor samples and valued their clinical significance. Their expression was detected by a series of techniques including pathological tissue microarray technology, immunohistochemistry(IHC) and immunofluorescent staining. Results: We found B7-H4 was expressed in 63.2%(55/87)of the colorectal cancer sampled;PKC-δ was detected in 65.5%(57/87)of the colorectal cancer sampled. Furthermore, the expression of B7-H4 was detected in the membrane of tumor cells from 40.2%(35/87) of the colorectal cancer sampled. Further analysis indicated that 34 of these PKC-δ+ samples expressed B7-H4. We also found B7-H4 and PKC-δ were co-expressed in colorectal cancer tissue samples(r=0.606,P<0.01). Moreover, in contrast to individual B7-H4 and PKC-δ expression, the membrane-expression of PKC-δ was evidently associated with the depth of tumor invasion, lymphatic metastasis, distant metastasis, and Dukes’ stage. Conclusion: B7-H4 was associated with PKC-δ that was abnormally expressed in CRC tissues, and the membrane-expression of PKC-δ correlated to CRC stages and tumor progression. It was suggested that PKC-δ regulated the expression of B7-H4 and the progression of colorectal cancer.

关键词： B7-H4 PKC colorectal cancer

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维特根斯坦的语言实践观

西北大学学报（哲学社会科学版） 2023年第5期53卷 5-16页

作者：张学广西北大学哲学学院、外国哲学研究中心陕西西安710127

在众多因素的联合作用尤其是拉姆齐和斯拉法的激烈批评之下,维特根斯坦逐渐走出《逻辑哲学论》的抽象语言实践观,在以《哲学研究》为代表作的后期哲学中展现了人类语言实践的广阔背景,并以此为依据揭示传统哲学由于误解语言用法而产生... 详细信息

在众多因素的联合作用尤其是拉姆齐和斯拉法的激烈批评之下,维特根斯坦逐渐走出《逻辑哲学论》的抽象语言实践观,在以《哲学研究》为代表作的后期哲学中展现了人类语言实践的广阔背景,并以此为依据揭示传统哲学由于误解语言用法而产生的种种问题。虽然维特根斯坦以“语言游戏”“家族相似”“生活形式”等独创性概念以及举例分析和对话辩驳的独特方法所开展的是一种“语法性研究”,但正是“人类学”转向才使他看清人类语言实践的本来面目,看清语言的社会性和历史性,从而为这种研究、为批判传统哲学奠定坚实的基础。《哲学研究》依据人类语言实践从简单到复杂的演进历程,循序渐进地揭示了以“名称对象”模型为主要误解图像而产生的种种哲学问题,不仅提供了反省哲学本性的经典案例,而且为哲学的实践转向注入新的活力。

关键词：维特根斯坦《逻辑哲学论》《哲学研究》语言实践

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后期维特根斯坦“语言实践”概念辨析

西北大学学报（哲学社会科学版） 2022年第5期52卷 50-59页

作者：张学广西北大学哲学学院陕西西安710127

维特根斯坦不仅是推动“语言转向”的中坚人物,而且在其后期哲学中一改前期对语言的看法,在生活之流中辨析语言的实际使用。他通过对语言实践进行详细的语法分析,探寻哲学问题产生的真正根源,带来对哲学性质和方法的颠覆性理解。后期维... 详细信息

维特根斯坦不仅是推动“语言转向”的中坚人物,而且在其后期哲学中一改前期对语言的看法,在生活之流中辨析语言的实际使用。他通过对语言实践进行详细的语法分析,探寻哲学问题产生的真正根源,带来对哲学性质和方法的颠覆性理解。后期维特根斯坦对语言实践的语法研究,成为20世纪下半叶哲学的“实践转向”的重要思想来源,不断启发人们对人类实践活动尤其与语言密切相关的实践活动的哲学探究。

关键词：后期维特根斯坦语言实践语法研究实践哲学

电网对称故障下基于active crowbar双馈发电机控制

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电机与控制学报 2009年第1期13卷 99-103页

作者：张学广徐殿国哈尔滨工业大学电气学院黑龙江哈尔滨150001

随着风力发电规模和风电机组单机容量不断增大,要求大型风电机组具有低电压穿越能力,因此需要研究三相对称故障下双馈风力发电机控制方法。在电网电压突然跌落时,由于双馈发电机中的电磁耦合关系,在定转子中感应出过电压过电流,为保护... 详细信息

随着风力发电规模和风电机组单机容量不断增大,要求大型风电机组具有低电压穿越能力,因此需要研究三相对称故障下双馈风力发电机控制方法。在电网电压突然跌落时,由于双馈发电机中的电磁耦合关系,在定转子中感应出过电压过电流,为保护转子侧变换器,需要通过crowbar来短路双馈发电机的转子。针对传统的passive crowbar的不足,采用active crowbar电路的控制方法。当电网故障造成双馈发电机转子过流时,开启active crowbar电路来旁路转子侧变换器。当转子电流下降到一定程度时断开crowbar,转子侧变换器恢复工作,此时双馈电机可以向电网同时提供有功无功支持。理论分析的基础上进行了仿真研究。仿真结果证实了采用active crowbar可以有效地实现双馈风力发电机的低电压穿越。

关键词：双馈发电机对称故障风力发电 active crowbar 低电压穿越