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Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第3期13卷 213-220页

作者： Shao-zhi zhang xiao-yi yu Guang-ming CHEN Institute of Refrigeration and Cryogenics Zhejiang University Hangzhou 310027 China

Osteochondral allografting has been proved to be a useful method to treat diseased or damaged areas of joint surfaces. Operational long-term stocks of grafts which supply a buffer between procurement and utilization would contribute to the commercialization or industrialization of this technology. Vitrification has been thought to be a promising method for successful preservation of articular cartilage （AC）, but high concentration cryoprotectants （CPAs） are used which may cause high cellular toxicity. An effective way to reduce CPA toxioity is to increase CPA concen- tration gradually while the temperature is lowered. Understanding the mechanism of （31~A permeation at subzero temperatures is important for designing the cryopreservation protocol. In this research, the permeation of dimethyl sulfoxide （MezSO） in ovine AC at subzero temperatures was studied experimentally. Pretreated AC discs were ex- posed in Me2SO solutions for different time （0, 5, 15, 30, 50, 80, and 120 min） at three temperature levels （-10, -20, and -30℃）. The Me2SO concentration within the tissue was determined by ultraviolet （UV） spectrophotometry. The diffusion coefficients were estimated to be 0.85×10-6, 0.48×10-8, and 0.27×10-6 cm2/s at -10, -20, and -,30℃, respectively, and the corresponding activation energy was 29.23 kJ/mol. Numerical simulation was performed to com- pare two Me2SO addition protocols, and the results demonstrated that the total loading duration could be effectively reduced with the knowledge of permeation kinetics.

关键词： Articular cartilage Vitrification Dimethyl sulfoxide Permeation Diffusion coefficient Subzero temperature

Tryptophan Modification and Fluorescence Spectrum of Hyaluronidase

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Chinese Chemical Letters 2005年第11期16卷 1492-1494页

作者： Li Rong TENG yu Zhuo CHU xiao Ping zhang xiao Kun yu Jing WANG Song HAN Bo Xun zhang Hao FAN Lan Ying LIU College of Life Science Jilin University Changchun 130023

Tryptophan residues in hyaluronidase （HAase） were modified by N-bromosuccinimide （NBS）, the results indicated that there were eleven tryptophan residues in HAase, one of which was exposed and essential for the activity of the enzyme. The study on fluorescence quenching showed that KI could not quench all of the fluorescence from Trp residues in HAase, while acrylamide （Acr） could quench almost all of the fluorescence from Trp residues in HAase. The collisional quenching constants （KD） of HAase at different concentrations of Acr were calculated in terms of Stern-Volmer equation. The results implied that some of the Trp residues were buried in the interior of HAase and the Trp residue on the surface of HAase was not located in the hydrophobic pocket.

关键词： Hyaluronidase (HAase) tryptophan (Trp) fluorescence spectrum.

Association of C-reactive Protein with Cardiovascular Outcomes: A Mendelian Randomization Study in the Japanese Population

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Biomedical and Environmental Sciences 2022年第2期35卷 126-132页

作者： CAO Ming Yang LIU Di zhang xiao yu TIAN Qiu yue zhang Qun WANG You Xin Beijing Key Laboratory of Clinical Epidemiology School of Public HealthCapital Medical UniversityBeijing 100069China Centre for Biomedical Information Technology Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055GuangdongChina Department of Anesthesiology Sanbo Brain HospitalCapital Medical UniversityBeijing 100093China Chinese Center for Disease Control and Prevention Beijing 102206China Centre for Precision Medicine School of Medical and Health SciencesEdith Cowan UniversityPerth 60127Australia

Objective Traditional epidemiological studies have shown that C-reactive protein(CRP)is associated with the risk of cardiovascular diseases(CVDs).However,whether this association is causal remains ***,Mendelian randomization(MR)was used to explore the causal relationship of CRP with cardiovascular outcomes including ischemic stroke,atrial fibrillation,arrhythmia and congestive heart *** We performed two-sample MR by using summary-level data obtained from Japanese Encyclopedia of Genetic association by Riken(JENGER),and we selected four single-nucleotide polymorphisms associated with CRP level as instrumental *** estimates were calculated with the inverse-variance weighted(IVW),penalized weighted median and weighted ***-Egger regression was used to explore *** No significant causal association of genetically determined CRP level with ischemic stroke,atrial fibrillation or arrhythmia was found with all four MR methods(all Ps>0.05).The IVW method indicated suggestive evidence of a causal association between CRP and congestive heart failure(OR:1.337,95%CI:1.005–1.780,P=0.046),whereas the other three methods did *** clear pleiotropy or heterogeneity were *** Suggestive evidence was found only in analysis of congestive heart failure;therefore,further studies are ***,no causal association was found between CRP and the other three cardiovascular outcomes.

关键词： Mendelian randomization C-reactive protein Cardiovascular diseases East Asians Causal inference

Identification of a clinical compound losmapimod that blocks Lassa virus entry

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中国药理学与毒理学杂志 2019年第10期 789-790页

作者： zhang xiao-yu GUO Ying Department of Pharmacology Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College

OBJECTIVE To identify small molecule compounds with activity against the entry of Lassa virus（LASV） and study their antiviral mechanism. METHODS An HIV based pseudotyped Lassa virus（LASV-GP/HIVluc） was used to evaluate 102 clinical investigative drugs for anti-LASV entry activity. Twelve HIV based pseudotyped are naviruses were used to evaluate the broadspectrum anti-arenavirus activity of compounds. RNAi was used to evaluate the effect of specific cel ular pathway on the anti-LASV activity of compounds. Time-of-addition assay and cell-cell fusion assay were used to identify the specific step in which compounds exert their antiviral effect. Site-directed mutagenesis was employed to identify the molecular target of the compounds. RESULTS This study identified the clinical investigative P38 MAPK inhibitor losmapimod with activity against LASV entry, with an EC50of 3.68 μmol·L-1. Losmapimod inhibited the entry of four lineages of LASV, with EC50of 0.9-2.7 μmol·L-1, and it has no effect on the entry of eight other human pathogenic arenaviruses(EC50>30 μmol · L-1). Losmapimod retained its anti-LASV activity on P38 down-regulated cells. Losmapimod has no effect on LASV attachment on cell surface; instead, it inhibited LASV-GP mediated viral fusion. Losmapimod lost its anti-LASV activity on SSP-TM mutated pseudotyped LASV. CONCLUSION Losmapimod specifically inhibits the entry of LASV through targeting the SSP-GP2 interface of LASV glycoprotein, blocking the LASV-GP mediated viral fusion, thereby thwarting LASV entry into host cells.

关键词： drug repurposing Lassa virus losmapimod entry inhibitors SSP-GP2

A One-dimensional Cobalt Coordination Polymer Based on Biphenyl-2,3,3',5'-tetracarboxylic Acid and N-donor Ancillary Ligands: Crystal Structure and Magnetic Property

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Chinese Journal of Structural Chemistry 2015年第10期34卷 1558-1564页

作者： LI Rong-Fang WANG yu-Fang LIU Xin-Fang FENG Xun zhang xiao-yu DU De-Guang College of Chemistry and Chemical Engineering Luoyang Normal University Food and Bioengineering Department Henan University of Science and Technology Luoyang Huier Nano Science and Technology Co Ltd

Hydrothermal reactions of biphenyl-2,3,3A,5A-tetracarboxylic acid（H4bptc） with cobalt salt in the presence of 1,4-bis（2-pyridylmethyl）piperazin（bpmp） afforded one novel coordination polymer, namely, [Co（H2bptc）（bpmp）0.5（H2O）]n（1）. Its structure was established by single-crystal X-ray diffraction analysis and further characterized by elemental analysis, IR spectra and TG analysis. Complex 1 crystallizes in monoclinic, space group P21/c with a = 11.4839（11）, b = 16.6690（16）, c = 11.5559（11） A, V = 2201.8（4） A3, Mr = 539.35, Dc = 1.627 g/cm^3, μ（MoK α） = 0.841 mm-1, F（000） = 1108, Z = 4, the final R = 0.0309 and w R = 0.0705 for 4090 observed reflections（I 2σ（I））. Complex 1 displays a one-dimensional（1D） chain bridged by bpmp. Two carboxylic groups of H4 bptc ligand adopt μ01-η1：η1 and μ1-η1：η coordination modes to bridge adjacent Co（Ⅱ） ions together with bpmp ligand to give alternately arranged left- and right-handed helical chains. In addition, variable-temperature magnetic susceptibility measurements indicate that complex 1 shows weak antiferromagnetic interactions between the adjacent Co（Ⅱ） ions.

关键词： cobalt polymer diphenyl-2,3,3' 5'-tetracarboxylic acid 1,4-bis(2-pyridylmethyl)piperazin magnetic property

Synthesis and characterization of novel polyimides based on thiazole-containing diamine

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Chinese Chemical Letters 2007年第10期18卷 1203-1204页

作者： Xin Zhao Yan Feng Li Shu Jiang zhang xiao Long-Wang yu Shao State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringInstitute of Biochemical Engineering ＆ Environmental Technology Lanzhou University Lanzhou 730000 China

A new kind of aromatic diamine monomer containing thiazole unit, 2-amino-5-（4-aminophenyl）-thiazole （AAPT）, was synthesized in three steps, starting from 4-nitroacetophenone. A novel thiazole-containing polyimide was prepared via the polycondensation of AAPT with 6FDA by one-step method. The resulting polyimide exhibits excellent solubility, film-forming capability and high thermal resistance.

关键词： Thiazole Thiazole-containing monomer Synthesis Aromatic diamine

Synthesis and Photovoltaic Properties of Non-fullerene Solution Processable Small Molecule Acceptors

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Chemical Research in Chinese Universities 2013年第3期29卷 596-599页

作者： LI Hui LIU Zhao-yang zhang xiao-yu YAO Shi-yu WEN Shan-peng TIAN Wen-jing State Key Laboratory of Supramolecular Structure and Materials Jilin University Changchun 130012 P. R. China

Two non-fullerene small molecules, BT-C6 and BT-C12, based on the vinylene-linked benzothiadiazole-thiophene（BT） moiety flanked with 2-（3,5,5-trimethylcyclohex-2-en-l-ylidene）malononitrile have been synthesized and characterized by solution/thin film UV-Vis absorption, photoluminescence（PL）, and cyclic voltammetry（CV） measurements. The two molecules show intense absorption bands in a wide range from 300 nm to 700 nm and low optical band- gaps for BT-C6（1.60 eV） and for BT-C12（1.67 eV）. The lowest unoccupied molecular orbital（LUMO） levels of both the molecules are relatively higher than that of [6,6]-phenyl C61 butyric acid methyl ester（PCBM）, promising high open circuit voltage（Voc） for photovoltaic application. Bulk heterojunction（BHJ） solar cells with poly（3-hexylthiophene）（P3HT） as the electron donor and the two molecules as the acceptors were fabricated. Under 100 mW/cm2 AM 1.5 G illumination, the devices based on P3HT：BT-C6（1：1, mass ratio） show a power conversion efficiency（PCE） of 0.67%, a short-circuit current（Jsc） of 1.63 mA/cm2, an open circuit voltage（Vow） of 0.74 V, and a fill factor（FF） of 0.56.

关键词： Non-fullerene small molecule Electron acceptor Bulk heterojunction

Influence of Ligand with Fine Difference at Donor Site on MMCT Property in Binuclear Mixed Valence Complexes

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Chinese Journal of Structural Chemistry 2021年第2期40卷 207-216,147页

作者： LI Su-Hua YANG yu-Ying zhang yu-xiao WU Xin-Tao SHENG Tian-Lu State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China College of Chemistry Fuzhou UniversityFuzhou 350002China University of Chinese Academy of Sciences Beijing 100049China

To investigate how the electronic effect of ligand at donor site influences electronic communication or metal-to-metal charge transfer(MMCT)properties in similar mixed-valence(MV)complexes,a series of binuclear organometallic complexes,MeCp(dppe)RuCNFeCl_(3)(1),MeCp(PPh_(3))_(2)RuCNFeCl_(3)(2),Cp^(*)(dppe)FeCNFeCl_(3)(3),Cp^(*)(dppe)RuCNFeCl_(3)(4)and Cp^(*)(PPh_(3))_(2)RuCNFeCl_(3)(5),have been synthesized and *** electronic absorptions of these complexes show the presence of MMCT properties between RuⅡ or FeⅡ and FeⅢ ions,strongly supported by the theoretical *** increasing electron-donating ability of ligands(PPh3>dppe,Cp^(*)>MeCp)at donor site,the MMCT absorption bands are red-shifted,which expresses in the sequence of absorption bands with 1(500 nm),4(536 nm),2(542 nm),5(580 nm)from high-energy to ***,the MMCT absorption energy of 4(536 nm)is larger than that of 3(760 nm)due to the stronger electron-donating ability of FeⅡ than RuⅡ.Furthermore,these complexes belong to the Class Ⅱ systems according to the Robin and Day’s classification.

关键词： metal-to-metal charge transfer(MMCT) mixed-valence complexes ClassⅡ

Stk2,a Mitogen-Activated Protein Kinase from Setosphaeria turcica,Specifically Complements the Functions of the Fus3 and Kss1 of Saccharomyces cerevisiae in Filamentation,Invasive Growth,and Mating Behavior

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Journal of Integrative Agriculture 2013年第12期12卷 2209-2216页

作者： GU Shou-qin YANG Yang LI Po zhang Chang-zhi FAN yu zhang xiao-yu TIAN Lan HAO Zhi-min CAO Zhi-yan GONG xiao-dong FAN Yong-shan HAN Jian-min DONG Jin-gao Mycotoxin and Molecular Plant Pathology Laboratory Agricultural University of Hebei Institute of Plant Protection Hebei Academy of Agriculture and Forestry Sciences Tangshan Normal University

Setosphaeria turcica,an essential phytopathogenic fungus,is the primary cause of serious yield losses in corn; however,its pathogenic mechanism is poorly *** cloned STK2,a newly discovered mitogen-activated protein kinase gene with a deduced amino acid sequence that is 96% identical to MAK2 from Phaeosphaeria nodorum,56% identical to KSS1 and 57% identical to FUS3 from Saccharomyces *** deduce Stk2 function in *** and to identify the genetic relationship between STK2 and KSS1/FUS3 from ***,a restructured vector containing the open reading frame of STK2 was transformed into a fus3/kss1 double deletion mutant of *** results show that the STK2 complementary strain clearly formed pseudohyphae and ascospores,and the strain grew on the surface of the medium after rinsing with sterile water and the characteristics of the complementary strain was the same as the wild-type ***,STK2 complemented the function of KSS1 in filamentation and invasive growth,as well as the mating behavior of FUS3 in ***,however,its exact functions in *** will be studied in the future research.

关键词： Setosphaeria turcica MAPK filamentation invasive growth mating behavior