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Identification of leaf rust resistance genes in common wheat varieties from China and foreign countries

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Journal of Integrative Agriculture 2021年第5期20卷 1302-1313页

作者： LIU Yuan Takele Weldu GEBREWAHID zhang pei-pei LI Zai-feng LIU Da-qun College of Plant Protection Hebei Agricultural UniversityBaoding 071001P.R.China College of Agriculture Aksum UniversityShire-Indaslassie 314Ethiopia

Wheat leaf rust,triggered by Puccinia triticina Eriks(Pt),is among the most important diseases of wheat *** resistant varieties against leaf rust is the most effective,environmentally-friendly and economic way to control the *** the present study,66 wheat varieties form China and foreign countries were tested with 17 Pt races for gene postulation during the seedling stage in the *** the varieties were also planted to identify slow rusting responses to leaf rust at the adult plant stage in Baoding and Zhoukou field trials during the 2016/2017 to 2017/2018 cropping ***,12 closely linked molecular markers to known leaf rust resistance(Lr)genes were used for assessing all the *** results of both gene postulation and molecular marker identification showed that a total of eight Lr genes,Lr1,Lr10,Lr17,Lr20,Lr26,Lr34,Lr37 and Lr46,either singly or in combination were detected in 32 *** Lr genes were not identified in the remaining 34 *** varieties were found to have slow rusting *** resistance sources identified in this study can be used as resources for resistance against leaf rust in wheat breeding programs in China and the respective foreign countries.

关键词： wheat gene postulation molecular marker Lr gene

Combining Phytate/Ca^(2+) Fractionation with Trichloroacetic Acid/Acetone Precipitation Improved Separation of Low-Abundant Proteins of Wheat (Triticum aestivum L.) Leaf for Proteomic Analysis

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Journal of Integrative Agriculture 2013年第7期12卷 1123-1129页

作者： Muhammad A R F Sultan LIU Hui CHENG Yu-Feng zhang pei-pei ZHAO Hui-xian State Key Laboratory of Crop Stress Biology for Arid Areas Ministry of Science and Technology/College of Life Sciences Northwest A&F University

Proteomic assessment of low-abundance leaf proteins is hindered by the large quantity of ribulose-1,5-bisphosphate carboxylase/oxygenase （Rubisco） present within plant leaf tissues. In the present study, total proteins were extracted from wheat （Triticum aestivum L.） leaves by a conventional trichloroacetic acid （TCA）/acetone method and a protocol first developed in this work. Phytate/Ca2＋ fractionation and TCA/acetone precipitation were combined to design an improved TCA/acetone method. The extracted proteins were analysed by two-dimensional gel electrophoresis （2-DE）. The resulting 2-DE images were compared to reveal major differences. The results showed that large quantities of Rubisco were deleted from wheat leaf proteins prepared by the improved method. As many as （758±4） protein spots were detected from 2-DE images of protein extracts obtained by the improved method, 130 more than those detected by the TCA/acetone method. Further analysis indicated that more protein spots could be detected at regions of pI 4.00-4.99 and 6.50-7.00 in the improved method-based 2-DE images. Our findings indicated that the improved method is an efficient protein preparation protocol for separating low-abundance proteins in wheat leaf tissues by 2-DE analysis. The proposed protocol is simple, fast, inexpensive and also applicable to protein preparations of other plants.

关键词： Triticum aestivum L. Rubisco low-abundance protein phytate/Ca2+ two-dimensional gel electrophoresis plant leaf proteomics

An Evaluation of the Infection Status and Source of Subgroup J Avian Leukosis Virus in Cloned Free-Range Layers

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Journal of Integrative Agriculture 2013年第4期12卷 687-693页

作者： zhang pei-pei LIU Shao-qiong WANG Jian WANG Bo ZHAO Cheng-di zhang Yong-guang SUN Shu-hong College of Veterinary Medicine Shandong Agricultural University Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences

In recent years, subgroup J avian leukosis virus （ALV-J） has been found to frequently infect layers in China. This virus is responsible for economic losses due to both mortality and decreased performance in chickens. In this study, 45-d-old cloned flee-range layers were suspected to be infected with ALV and other immunosuppressive diseases because their feathers were unkempt and their growth rate was impaired. To estimate the infection status and determine the source of ALV-J in the flock, 30 cloacal swabs were randomly collected to measure the p27 antigen level by enzyme-linked immunosorbent assay （ELISA）. Among the birds that were tested, 87% （26/30） were positive. In addition, 6 anticoagulant blood samples were aseptically collected at random from the flock when the layers were 60 d old. These samples were centrifuged to obtain the leukocytes, which were then used to inoculate chicken embryo fibroblast （CEF） cells for the identification of ALV-J by indirect immunofluorescence （IFA）. Of the samples tested, 100% （6/6） were positive. The flock＇s production performance was also investigated, and 10 layers were necropsied to evaluate pathological changes at 115 d of age. The flock never laid eggs even though they reached the age of the first laying （110 d）. Furthermore, there were pathological changes present, including atrophy of the thymus and bursa of Fabricius, undeveloped ovaries, glandular stomach haemorrhage, and hepatosplenomegaly. Paraffin-embedded sections of intumescent liver and spleen were prepared for antigen localisation using IFA. Positive signals were prevalent in paraffin-embedded sections of the intumescent liver and spleen. Furthermore, provirus DNA was extracted from 4 cloned flee-range layers, and 2 patemal parents （HR native cocks）, and the gp85 gene of ALV-J was amplified by PCR to analyse the genetic variation. The results of the autogenous variation analysis showed that the 6 strains were 98.5-99.7% homologous. This study indicated that there

关键词： cloned free-range layers ALV-J infection status hereditary variation source exploration

Mapping of QTL conferring leaf rust resistance in Chinese wheat lines W014204 and Fuyu 3 at adult plant stage

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Journal of Integrative Agriculture 2016年第1期15卷 18-28页

作者： QI Ai-yong zhang pei-pei ZHOU Yue YAO Zhan-jun LI Zai-feng LIU Da-qun Department of Plant Pathology College of Plant Protection Agricultural University of Hebei Department of Biochemistry Baoding University College of Agronomy Agricultural University of Hebei

Wheat leaf rust is a destructive foliar disease of common wheat （Triticum aestivum L.） worldwide. The most effective, economical s to control the disease is growing resistant cultivars with adult plant resistance （APR）. The Chinese wheat tines W014204 and Fuyu 3 showed high leaf rust resistance in the field. To identify leaf rust APR genes in the two lines, two mapping populations with 215 and 163 F2：3 lines from the crosses W014204/Zhengzhou 5389 and Fuyu 3/Zhengzhou 5389, respectively, were phenotyped for leaf rust severities during the 2010-2011, 2011-2012 and 2012-2013 cropping seasons in the field at Baoding, Hebei Province, China. A total of 1 215 SSR markers were used to identify the quantitative trait loci （QTLs） for leaf rust APR in the two populations. In the W014204/Zhengzhou 5389 population, three QTLs were detected and designated as ***-lBL.1, ***-2BS.1 and ***-7DS, and explained 2.9-8.4, 11.5-38.3 and 8.5-44.5% of the phenotypic variance, respectively; all the resistance alleles at these loci were derived from W014204. In the Fuyu 3/Zhengzhou 5389 population, three QTLs, ***-lBL.2, ***-2BS.2 and ***-7BL, explained 12.0-19.2, 22.3-38.9 and 4.1-4.3% of the phenotypic variance, respectively, and all resistance alleles were contributed by Fuyu 3. Based on chromosome positions of closely linked markers, both ***-lBL. 1 and ***-lBL.2 are Lr46, and ***-7DS is Lr34. ***-7BL was mapped on chromosome 7BL near to Lr68 and they are likely the same gene. Based on chromosome positions, pedigree and field reactions, the two 2BS QTLs are different from all the known APR genes and are likely to be newAPR QTL for leaf rust. These QTLs and their closely linked markers are potentially useful for improving leaf rust resistance in wheat breeding.

关键词： Triticum aestivum Puccinia triticina APR SSR marker

Seedling and adult plant resistance to leaf rust in 46 Chinese bread wheat landraces and 39 wheat lines with known Lr genes

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Journal of Integrative Agriculture 2019年第5期18卷 1014-1023页

作者： zhang pei-pei Takele Weldu Gebrewahid ZHOU Yue LI Qing-luo LI Zai-feng LIU Da-qun Department of Plant Pathology College of Plant Protection Hebei Agricultural University College of Agriculture Aksum University-Shire Campus College of Biochemistry and Environmental Engineering Baoding University

Wheat leaf rust,caused by Puccinia triticina(Pt),is an important foliar disease that has an important influence on wheat *** most economic,safe and effective way to control the disease is growing resistant *** the present study,a total of 46 wheat landraces and 34 wheat lines with known Lr(leaf rust resistance)genes were inoculated with 16Pt pathotypes for postulating seedling resistance gene(s)in the *** cultivars and five wheat differential lines with adult plant resistance(APR)genes(Lr12,Lr22b,Lr34,Lr35 and Lr37)were also evaluated for identification of slow rusting resistance in the field trials in Baoding,Hebei Province of China in the 2014–2015 and 2015–2016 cropping ***,10 functional molecular markers closely linked to 10 known Lr genes were used to detect all the wheat *** showed that most of the landraces were susceptible to most of the Pt pathotypes at seedling ***,Lr1 was detected only in *** field experimental test of the two environments showed that 38 landraces showed slow rusting *** cultivars possessed Lr34 but none of the landraces contained Lr37 and *** genes namely,Lr9,Lr19,Lr24,Lr28,Lr29,Lr47,Lr51 and Lr53 were effective at the whole plant ***18,Lr36 and Lr45 had lost resistance to part of pathotypes at the seedling stage but showed high resistance at the adult plant ***34 as a slowing rusting gene showed good resistance in the *** race-specific APR genes Lr12,Lr13,Lr35 and Lr37 conferred good resistance in the field *** race-specific genes,Lr2b,Lr2c,Lr11,Lr16,Lr26,Lr33 and LrB had lost *** 38 landraces showed slow rusting resistance to wheat leaf rust can be used as resistance resources for wheat resistance breeding in China.

关键词： Triticum aestivum L. wheat leaf rust gene postulation adult plant resistance

K-Domain Splicing Factor OsMADS1 Regulates Open Hull Male Sterility in Rice

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Rice science 2015年第5期22卷 207-216页

作者： SUN Lian-ping zhang Ying-xin zhang pei-pei YANG Zheng-fu ZHAN Xiao-deng SHEN Xi-hong zhang Zhen-hua HU Xia XUAN Dan-dan WU Wei-xun LI Zi-he CAO Li-yong CHENG Shi-hua National Center for Rice Improvement / Key Laboratory for Zhejiang Super Rice Research China National Rice Research Institute College of Agronomy Shenyang Agricultural University

We identified the rice floral organ development mutant, termed as open hull and male sterile 1 （ohms1）, from the progeny of the indica restorer line Zhonghui 8015 treated with 60Co γ-ray irradiation. The ohms1 mutant exhibited an open hull and lemma- and palea-like structure conversion between the anthers and stigma, which resulted in the ohms1 mutant spikelet showing‘tridentate lemma＇. The ohms1 mutant was entirely sterile but had 60%-70% fertile pollen. Genetic analysis and gene mapping showed that ohms1 was controlled by a single recessive gene, and the mutant gene was fine-mapped to a 42-kb interval on the short arm of chromosome 3 between markers KY2 and KY29. Sequence analysis of the four open reading frames in this region revealed that the mutant carried a single nucleotide transformation （A to G） at the last base of the fifth intron, which was likely corresponded to ohms1 phynotype, in an MIKC type MADS-box gene OsMADS1 （LOC_Os03g11614）. Enzyme digestion and cDNA sequencing further indicated that the variable splicing was responsible for the deletion of the sixth exon in ohms1, but no structural changes in the MADS domain or amino acid frame shifts appeared. Additionally, real-time fluorescent quantitative PCR analysis showed that the OsMADS1 expression level decreased significantly in the ohms1 mutant. The expression levels of rice flowering factors and floral glume development-related genes also changed significantly. These results demonstrate that OsMADS1 may play an important role in rice floral organ development, particularly in floral glume development and floret primordium differentiation.

关键词： rice open hull male sterile ohms1 gene mapping alternative splicing floral organ

Supply Chain Management System Model of Virtual Enterprises Based on Multi-Agent

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International Journal of Plant Engineering and Management 2008年第3期13卷 167-173页

作者： LI Zhen zhang pei-pei School of Mechanical Engineering Anhui University of Technology and Science Wuhu 241000P. R. China

Based on the analysis of a virtual enterprise and the development of supply chain management, their integration is proposed. Then, the difference between multi-agent system modeling method and the traditional modeling method is analyzed, and a method based on Java agent framework for multi-agent systems（ JAFMAS） is proposed. By using this method the virtual enterprise＇ s supply chain management system model is established.

关键词： virtual enterprise supply chain management multi-agent JAFMAS system model

Depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 335-345页

作者： Feng zhang Chenyang zhang Linlin Wu Wei Sun Hongliang zhang Jianhua Chen Yong pei Songjiang Li Engineering Research Center of Ministry of Education for Carbon Emission Reduction in Metal Resource Exploitation and Utilization Hunan International Joint Research Center for Efficient and Clean Utilization of Critical Metal Mineral ResourcesSchool of Minerals Processing and BioengineeringCentral South UniversityChangsha 410083China State Key Laboratory of Mineral Processing Beijing General Research Institute of Mining&MetallurgyBeijing 100160China Hunan Provincial Key Laboratory of Micro&Nano Materials Interfaces Science College of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China School of Resources Environment and MaterialsGuangxi UniversityNanning 530004China Department of Chemistry Xiangtan UniversityXiangtan 411105China China Railway Resource Group Co.Ltd Beijing 102300China

The depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite still lacked in-depth ***,the depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite was further systematically investigated with experiments and density functional theory(DFT)*** X-ray photoelectric spectroscopy(XPS)results,DFT calculation results,and frontier molecular orbital analysis indicated that sulfite ions were difficult to be adsorbed on sphalerite surface,suggesting that sulfite ions achieved depression effects on sphalerite through other non-adsorption ***,the oxygen content in the surface of sphalerite treated with sulfite ions in creased,which enhanced the hydrophilicity of the sphalerite and further increased the difference in hydrophilicity between sphalerite and ***,sulfite ions were chelated with lead ions to form PbSO_(3)in *** hydrophilic PbSO_(3)was more easily adsorbed on sphalerite than *** interaction between sulfite ions and lead ions could effectively inhibit the activation of *** addition the UV spectrum showed that after adding sulfite ions,the peak of perxanthate in the sphalerite treated xanthate solution was significantly stronger than that in the galena with xanthate solution,indicating that xanthate interacted more readily with sulfite ions and oxygen mo lecules within the sphalerite system,leading to the formation of ***,sulfite ions hardly depressed the flotation of ga lena and could promote the flotation of galena to some *** study deepened the understanding of the depression mechanism o sulfite ions on sphalerite and Pb^(2+)activated sphalerite.

关键词： sphalerite galena sulfite ion density functional theory depression mechanism

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试论民国时期女子实业的创办及成功原因

妇女研究论丛 2013年第4期 79-86页

作者：张佩佩重庆交通大学思政部重庆400074

民国时期,随着经济的发展、社会的进步和女子解放运动的进行,女子实业蔚然兴起并逐步得到发展。从从事的行业看,主要有农业、工商业、金融业和服务业等。此时的女子实业取得了较为显著的成绩是多因素综合作用的结果。从经营策略分析,能... 详细信息

民国时期,随着经济的发展、社会的进步和女子解放运动的进行,女子实业蔚然兴起并逐步得到发展。从从事的行业看,主要有农业、工商业、金融业和服务业等。此时的女子实业取得了较为显著的成绩是多因素综合作用的结果。从经营策略分析,能够根据国情提倡国货,发挥女性特色,具体的营销手段能做到科学、多样等都是其成功的原因。女子实业有着巨大的意义,不仅为女子自身求得了权力、利益和荣誉,还促进了社会物资生产的发展,冲击了既有的性别分工方式与性别角色的认定,改变了社会风气,促进了社会变革等,还为女性树立了榜样。

关键词：女子实业成功原因经营策略民国时期