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CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

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Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： lin Yin Yalan Xu jie Mu Yu Leng Lei Ma Yu Zheng Ruizhi li Yin Wang Peifeng li Hai Zhu Dong Wang Jing li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

A fast streptomycin sulfate sensing based on Nb2CTX functionalized titled fiber Bragg grating

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Optoelectronics Letters 2025年

作者： BI Mingpan liU jie zhang Yuchen li Xiaolan MIAO Yinping Tianjin Key Laboratory of Film Electronic & Communicate Devices，School of Integrated Circuit Science and Engineering，Tianjin University of Technology Key Laboratory of Quantum Optics and Intelligent Photonics， School of Science， Tianjin University of Technology

We proposed a fast and temperature self-compensation method to detect streptomycin sulfate aqueous solution concentration based on a 2D Nb2CTXMXene nanosheets functionalized tilted Bragg grating（N-TFBG）. The introduction of 2D Nb2CTXMXene nanosheets brought more than 100% increase in sensitivity and exhibited a high sensitivity of 253.8 pm/mg/mL and detection limit 80 μg/mL in range of 0.2-0.8 mg/mL. Due to high sensitivity and compact structure， N-TFBG sensor has a faster measure time than previous method; additionally， the sensor has simultaneous temperature measurement by detecting the cladding mode and the core mode resonance peak simultaneously. Our research will hopefully open a new research platform for online detection of streptomycin sulfate concentration.

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猪激素敏感脂酶和甘油三酯水解酶基因组织表达特性研究

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生物工程学报 2007年第5期23卷 831-835页

作者：王琦张利红张立杰杨公社西北农林科技大学动物科技学院动物脂肪沉积与肌肉发育实验室杨凌712100

以八眉猪为研究对象,采用RT-PCR和Western blot方法对猪激素敏感酯酶(HSL)和甘油三酯水解酶(TGH)基因组织表达特点进行了研究。RT-PCR半定量检测显示,HSL基因的mRNA在检测的7种组织中都有表达,其中在脂肪组织表达量较高,中等程度表达于... 详细信息

以八眉猪为研究对象,采用RT-PCR和Western blot方法对猪激素敏感酯酶(HSL)和甘油三酯水解酶(TGH)基因组织表达特点进行了研究。RT-PCR半定量检测显示,HSL基因的mRNA在检测的7种组织中都有表达,其中在脂肪组织表达量较高,中等程度表达于心脏、肝脏、肺、脾和肾脏。TGH基因在7种组织也均有表达,其中肝脏和脂肪组织表达量较高,心脏和肾脏次之,脾脏和肺脏表达量较低。Western blot检测显示,HSL基因在大网膜脂肪和皮下脂肪表达量最高,而在肾脏中没有检测到表达,其他组织中中度表达;TGH基因在大网膜脂肪、皮下脂肪、肝脏、肺脏和脾脏组织中表达,其中在脂肪组织和肝脏组织中表达量最高,而在心脏和肾脏中没有检测到表达。以上结果表明:HSL和TGH基因存在转录后调控,这可能与其在不同组织中的功能差异有关。

关键词：猪 HSL基因 TGH基因组织表达

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传统出版知识服务转型探究

编辑之友 2020年第1期 46-50页

作者：张利洁张艳彬兰州大学新闻与传播学院

知识服务市场日趋兴起,传统出版依托内容授权服务、多元主体合作、自建一体化综合平台等形式布局知识服务领域。在此过程中,传统出版面临规模化建设、版权隐患和用户立场等现实挑战。传统出版机构通过搭建合理的内容审核体系、开拓立体... 详细信息

知识服务市场日趋兴起,传统出版依托内容授权服务、多元主体合作、自建一体化综合平台等形式布局知识服务领域。在此过程中,传统出版面临规模化建设、版权隐患和用户立场等现实挑战。传统出版机构通过搭建合理的内容审核体系、开拓立体化的推广系统,以及立足市场实现价值增值等策略,力图实现从传统出版到知识服务的转型。

关键词：传统出版知识服务创新增值

基于损伤控制方法的大理岩非线性剪胀特性试验研究

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岩土力学 2015年第4期36卷 981-987页

作者：汪斌朱杰兵邬爱清张利洁黄书岭长江科学院水利部岩土力学与工程重点实验室湖北武汉430010

剪胀角是描述岩石体积膨胀扩容的常用参数,在非关联流动法则中,连续介质理论通常假设剪胀角为0;在关联流动法则中,其值恒定且等于内摩擦角。岩石三轴压缩全过程体应变曲线表明,其体积剪胀性依赖于围压和塑性参量,破坏过程中不仅其特征... 详细信息

剪胀角是描述岩石体积膨胀扩容的常用参数,在非关联流动法则中,连续介质理论通常假设剪胀角为0;在关联流动法则中,其值恒定且等于内摩擦角。岩石三轴压缩全过程体应变曲线表明,其体积剪胀性依赖于围压和塑性参量,破坏过程中不仅其特征强度随围压和塑性参量呈非线性变化,而且剪胀特性也表现出非线性特征。基于塑性力学理论,针对锦屏大理岩损伤控制的全过程三轴加、卸载试验,采用双参数非线性函数拟合方法建立了能同时考虑围压效应和塑性参量的非线性剪胀角模型。结果表明,对于大理岩、中硬岩,在破坏过程中扩容行为强烈依赖围压和岩石塑性参量,均表现出先快速增加至峰值后,随着塑性变形增加逐渐减小的非线性演化规律。提出的双参数非线性剪胀角模型很好地描述了岩石破坏过程中的体积扩容特性,其结果对于研究地下工程围岩应力变化诱发的围岩剪胀破坏机制、体积扩容膨胀区范围预测和围岩支护的合理设计均具有一定的理论和工程应用价值。

关键词：剪胀角损伤控制塑性参量三轴加卸载试验非线性剪胀角模型

从免费惯性到付费变现——数字环境下知识传播模式的变化研究

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编辑之友 2017年第12期 50-53页

作者：张利洁张艳彬兰州大学新闻与传播学院甘肃兰州730000

数字环境下,传统的知识传播格局受到冲击。知识传播模式发生着巨大的变化,将知识延伸为一种"经济"的趋势不可违抗。文章梳理了数字环境下多元化的知识付费模式,从认知盈余和粉丝效应阐释了推动知识传播模式变化的动因。多元化的知识付... 详细信息

数字环境下,传统的知识传播格局受到冲击。知识传播模式发生着巨大的变化,将知识延伸为一种"经济"的趋势不可违抗。文章梳理了数字环境下多元化的知识付费模式,从认知盈余和粉丝效应阐释了推动知识传播模式变化的动因。多元化的知识付费模式也给知识传播环境带来重大影响,包括推动知识付费群体的形成、出版社向全方位知识服务转型、降低传播噪音扩张、"长尾"孕育细分的利基市场等方面。

关键词：知识传播知识付费数字环境

Cyano migration-mediated radical-polar crossover cyclopropanation

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Science China Chemistry 2025年第01期 241-248页

作者： Ziqiang Wang Yasu Chen jie li Chen Zhu Key Laboratory of Organic Synthesis of Jiangsu Province College of Chemistry Chemical Engineering and Materials ScienceSoochow University Frontiers Science Center for Transformative Molecules Zhangjiang Institute for Advanced Study and Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs Shanghai Jiao Tong University

We report a novel cyano migration-mediated radical-polar crossover process, whereby both the sulfonylcyanation of aliphatic alkenes and the formation of cyclopropanes are concurrently achieved. This transformation yields a diverse array of multisubstituted cyclopropanes under mild photocatalytic conditions. Mechanistic studies elucidate that the reaction proceeds via intramolecular nucleophilic substitution, leading to the formation of the cyclopropane derivatives.

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Leader-follower: Human-centered intention-guided controller for novel Superlimb with application to load-carrying scenarios

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Science China Technological Sciences 2025年第1期 325-338页

作者： Qinghua zhang Xianglong li Hongwei JING Kerui SUN Yuancheng li Sikai ZHAO Lele li Haotian JU Ziqi WANG Rui WU Tianjiao ZHENG jie ZHAO Yanhe ZHU The State Key Laboratory of Robotics and System Harbin Institute of Technology The Learning Algorithms and Systems Laboratory School of Engineeringécole Polytechnique Fédérale de Lausanne

Supernumerary robotic arms(Superlimb) are a new type of wearable robot that works closely with humans as a third hand to augment human operation capability. Accurate conveyance of wearers' intentions, allocation of roles, and humancentered interaction considerations are the key points in the process of human–Superlimb collaboration. This paper proposes a human-centered intention-guided leader–follower controller that relies on the dynamic modeling of Superlimb with application to load-carrying scenarios. The proposed leader–follower controller takes the human as the leader and the Superlimb as the follower, achieving effective information communication, autonomous coordination, and good force compliance between Superlimb, humans, and the environment under human safety assurance. First, the human–Superlimb dynamic system is modeled to achieve force interaction with the environment and wearer. Second, to achieve the precise intention extraction of humans, pose data from five visual odometry sensors are fused to capture the human state, the generalized position, the velocity of hands, and the surface electromyography signals from two myoelectric bracelets sensors are processed to recognize the natural hand gestures during load-carrying scenarios by a designed Swin transformer network. Then, based on the real-time distance detection between human and mechanical limbs, the security assurance and force-compliant interaction of the human–Superlimb system are realized. Finally, the human hand muscle intention recognition, human–robot safety strategy verification,and comparative load-carrying experiments with and without the proposed method are conducted on the Superlimb *** showed that the task parameters are well estimated to produce more reasonable planning trajectories, and Superlimb could well understand the wearer's intentions to switch different Superlimb actions. The proposed sensor-based human–robot communication framework motivates future studies of o

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基于自动站资料的深圳灰霾特征对比分析

中国环境监测 2013年第6期29卷 39-43页

作者：施何俊张丽张立杰中山大学环境科学与工程学院广东广州510275 深圳市国家气候观象台广东深圳518040

利用深圳自动气象站的气象要素和深圳大气成分监测系统采集的大气成分数据，分析了深圳城区和郊区灰霾季节变化、日变化差异和不同风向下污染物浓度差异，结果表明，城区由于人类活动频繁导致灰霾日比郊区多，以轻微灰霾偏多为主。秋、... 详细信息

利用深圳自动气象站的气象要素和深圳大气成分监测系统采集的大气成分数据，分析了深圳城区和郊区灰霾季节变化、日变化差异和不同风向下污染物浓度差异，结果表明，城区由于人类活动频繁导致灰霾日比郊区多，以轻微灰霾偏多为主。秋、冬季城区冷空气活动频繁、能源消耗大，灰霾出现频率是郊区的1～2倍；春季冷空气和海上暖湿气流容易形成对峙，沿海颗粒物更容易吸湿增长，郊区灰霾频率反而比城区高25％；夏季对流强、降水频密，城郊差异最小。城区灰霾频率受早晚交通高峰期影响，日变化呈双峰型。而郊区受太阳辐射和光化学反应影响大，呈单峰型。偏北风条件下污染物浓度明显升高，偏南风带来的清洁空气使得颗粒物浓度降幅明显。

关键词：城郊灰霾日变化吸湿增长