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Transpositional behaviour of the Ds element in the Ac/Ds system in rice

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Chinese Science Bulletin 2007年第20期52卷 2789-2796页

作者： LIU Fang zhang XiangQian zhang ZeMin CHEN ZhaoGui ZHU HaiTao WANG Jiang zhang jingliu zhang GuiQuan College of Agriculture South China Agricultural University Guangzhou 510642 China National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science Chinese Academy of Sciences Shanghai 200032 China

Insertional mutagenesis based on maize Activator/Dissociator (Ac/Ds) transposons is becoming a ma- jor approach used to produce a saturated mutant collection in rice. In this research, Ds-T-DNA trans- formed homozygotes were crossed with Ac-T-DNA transformed homozygotes in order to establish an Ac/Ds transposon system in rice. The successive investigation of Ds transposition from F1 to F5 gen- erations indicated that the frequencies of germinal transposition increased over successive genera- tions and reached 54.2% in F3 generation. The Ds transposition pattern revealed that a Ds transposition induced an approximately 170-bp deletion of T-DNA sequence and another Ds transposition carried a 272-bp T-DNA sequence. Using thermal asymmetric interlaced PCR (TAIL-PCR), some flanking se- quences of the Ds element were amplified. Analyses of 17 Ds-flanking sequences showed that five Ds were inserted into gene regions. The Ds could transpose not only to the linked sites but also to the unlinked sites. The frequency of inter-chromosomal transposition of Ds was 33.3%.

关键词：置换行为稻米激活剂幼芽

Introduction of antisense waxy gene into main parent lines of indica hybrid rice

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Chinese Science Bulletin 2002年第14期47卷 1192-1197页

作者： CHEN Xiuhua LIU Qiaoquan WANG Zongyang WANG Xingwen CAI Xiuling zhang jingliu GU Minghong Key Laboratory of Biotechnology of Jiangsu Province Yangzhou UniversityYangzhou 225009China Shanghai Institute of Plant Physiology and Ecology Chinese Academy of ScienceShanghai 200032China

Amylose content in rice endosperm is one of the key determinants of rice eating and cooking quality, and the poor quality of indica hybrid rice is closely related to the high amylose level in rice grains. In order to improve the grain quality of the indica hybrid rice by genetic engineering, an antisense fragment of rice waxy gene, driven by the 5’-franking sequences of the rice waxy gene, was successfully introduced into three major parent lines of indica hybrid rice, all contain a high amylose level in the grains, via Agrobacte-rium, and more than 100 hygromycin-resistant plants were regenerated. The analysis of PCR amplification and Southern blots indicated that the T-DNA containing the antisense waxy gene had been integrated into the genome of transgenic rice plants. Most of the primary transgenic rice plants grew normally, and the mature seeds from these transgenic plants were performed for analysis of the amylose content. The results showed that the amylose content in the endosperm of some grains

关键词： transgenic rice indica rice maintain line antisense waxy gene amylose content.

Distribution of T-DNA carrying a Ds element on rice chromosomes

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Science China(Life Sciences) 2004年第4期47卷 322-331页

作者： WANG Jiang LI Lin WAN Xinshan AN Linsheng zhang jingliu National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology & EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200032China

Over 3000 rice plants with T-DNA carrying a Ds element were constructed by Agro-bacterium tumefaciens mediation. Using inverse PCR methodology, 590 unique right flanking sequences of T-DNA (Ds) were retrieved from independent transformants and classified into six main types on the basis of the origin of filler DNA between the right border of T-DNA and flanking sequence of rice genome. Type I sequences were the most common and showed canonical in-tegration that T-DNA right border was followed by rice genome sequence with or without filler DNA of no more than 50 bp, while type II sequences displayed a vector-genome combination that T-DNA right border was followed by a vector fragment and then connected with rice genome sequence. The location and distribution of 340 type I and II flanking sequences on the rice chromosome were determined using BLAST analysis. The 340 Ds insertions at an average in-terval of 0.8 megabase (Mb) constructed a basic framework of Ds starter points on whole rice chromosomes. The frequency of T-DNA (Ds) inserted into the exons of predicted genes on chromosome one was 21%. Knowledge of T-DNA (Ds) locations on chromosomes will prove to be a useful resource for isolating rice genes by Ds transposon tagging as these Ds insertions can be used as starting lines for further mutagenesis.

关键词： Ds element T-DNA mutagenesis rice(Oryza sativa L.).

Albino Leaf 1 that encodes the sole octotricopeptide repeat protein is responsible for chloroplast development in rice

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Albino Leaf 1 that encodes the sole octotricopeptide repeat ...

2016年全国植物生物学大会

作者： Zemin zhang Jianjie Tan Zhenying Shi Qingjun Xie Yi Xing Changhong Liu Qiaoling Chen Haitao Zhu Jiang Wang jingliu zhang Guiquan zhang State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Provincial Key Lab of Plant Molecular BreedingSouth China Agricultural University Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences

Chloroplast,the photosynthetic organelle in plants,plays a crucial role in plant development and growth through manipulating the capacity of photosynthesis. However,the regulatory mechanism of chloroplast development still remains elusive. Here,we characterized a mutant with defective in chloroplasts in rice,termed albino leaf 1(al1),which exhibits distinct albino phenotype in leaves,eventually leading the al1 seedling to lethal. Electronic microscopy observation demonstrated that the numbers of thylakoids was reduced and the structure of thylakoids was disrupted in the al1 mutant during rice development,which eventually led to the breakdown of chloroplast. Molecular cloning revealed that AL1 encodes the sole Octotricopeptide Repeat Protein(RAP) in rice. Genetic complementation of the Arabidopsis rap mutants indicated that AL1 protein is a functional RAP. Further analysis illustrated that three transcript variants were present in the AL1 gene and the altered splices were occurred at the 3′UTR of AL1 transcript. In addition,our results also indicate that disruption of AL1 gene results in the altered expression of chloroplast associated genes. Consistently,proteomic analysis demonstrated that the abundances of photosynthesis associated proteins are significantly altered,as well as a group of metabolism associated proteins. More specifically,we found that the loss of AL1 resulted in the altered abundances of ribosomal proteins,suggesting that RAP likely also regulates the homeostasis of ribosomal proteins in rice in addition to the r RNA. Taken together,we conclude that the AL1,particularly the AL1 a and c isoform,plays an essential role in chloroplast development in rice.

关键词： chloroplast development RAP AL1 gene OPR

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针灸治疗膝关节骨性关节炎临床研究概况

实用中医内科杂志 2021年第6期35卷 116-119页

作者：张荆柳柳州市潭中人民医院广西柳州545007

简述了针灸治疗膝关节骨性关节炎(osteoarthritis,OA)临床研究概况。膝关节OA目前仍没有明确的治疗方法,膝关节OA诊疗指南建议的治疗方法包括药物治疗、非药物治疗和手术治疗。针灸作为传统中医药疗法,在膝关节骨性关节炎临床治疗中有... 详细信息

简述了针灸治疗膝关节骨性关节炎(osteoarthritis,OA)临床研究概况。膝关节OA目前仍没有明确的治疗方法,膝关节OA诊疗指南建议的治疗方法包括药物治疗、非药物治疗和手术治疗。针灸作为传统中医药疗法,在膝关节骨性关节炎临床治疗中有其独特的优势。查阅针灸治疗膝骨性关节炎近10年相关文献,发现针灸治疗本病通过对膝关节周围的穴位刺激而促进膝关节部位血液循环,可以加强炎性水肿的吸收,有利于增加膝关节的活动度,缓解患者的疼痛,从而延缓病情的发展。

关键词：膝关节骨性关节炎针灸疗法临床研究综述

利用极性反转合成苄叉氯亚胺-1-α及苄叉氯亚胺的催化分解

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高等学校化学学报 1987年第3期8卷 246-248页

作者：张景榴福建师范大学化学系

文献报导二苯基N-无取代硫亚胺(Ⅰ)和醛作用可得对应的腈,曾设想其机理如下Ph_(2)S-NH+PhCHO→←[Ph_(2)S-N-Ph-C-OH-H-Ph_(2)S-N=C■]→Ph_(2)S+PhCN+H_(2)O已知苄叉氯亚胺可分解成相应的腈,考虑到它在结构上与瞬时中间体B相似,如果在... 详细信息

文献报导二苯基N-无取代硫亚胺(Ⅰ)和醛作用可得对应的腈,曾设想其机理如下Ph_(2)S-NH+PhCHO→←[Ph_(2)S-N-Ph-C-OH-H-Ph_(2)S-N=C■]→Ph_(2)S+PhCN+H_(2)O已知苄叉氯亚胺可分解成相应的腈,考虑到它在结构上与瞬时中间体B相似,如果在二苯硫醚共存下反应,可能形成结构上与B很相似的C。

关键词：催化分解亚胺二苯硫醚极性反转文献报导 Ⅰ) 中间体腈

CT联合CA125检测在卵巢癌诊断中的应用价值

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实用癌症杂志 2015年第9期30卷 1290-1292页

作者：张静柳黄俊吴歆华解放军425医院 572008

目的探讨应用CT与CA125联合诊断卵巢癌的价值。方法选取经临床手术病理证实为卵巢癌的60例患者纳入研究,并分别采用CT、CA125、CT联合CA125检测;并以临床手术病理诊断为标准来评价3组的诊断准确性。结果与手术病理结果比较,CT临床检查... 详细信息

目的探讨应用CT与CA125联合诊断卵巢癌的价值。方法选取经临床手术病理证实为卵巢癌的60例患者纳入研究,并分别采用CT、CA125、CT联合CA125检测;并以临床手术病理诊断为标准来评价3组的诊断准确性。结果与手术病理结果比较,CT临床检查诊断准确率为86.67%(52/60)、CA125检测准确率为68.33%(41/60)、CT与CA125联合诊断准确率为96.67%(58/60)。CT与CA125联合检出率明显高于其他2组(P0.05)。结论相比单一检测来说,CT与CA125联合检测诊断卵巢癌可提高临床诊断准确性,同时采用CT临床分期具有较高准确性,可为临床疾病诊断及治疗提供重要参考价值。

关键词：卵巢癌 CT CA125 诊断

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广东省普通高校师资队伍现状及建设对策

新疆农垦经济 2009年第10期 52-55页

作者：袁文才章劲柳华南农业大学广州510642

该文主要对1998年～2007年10年间广东省普通高校师资队伍的现状进行调查分析。结果表明:广东高校教师队伍在近10年获得了迅速的发展,数量基本充足,但同时也面临着数量结构性不足、学历层次仍待提升、职称结构、学缘结构还待优化、高层... 详细信息

该文主要对1998年～2007年10年间广东省普通高校师资队伍的现状进行调查分析。结果表明:广东高校教师队伍在近10年获得了迅速的发展,数量基本充足,但同时也面临着数量结构性不足、学历层次仍待提升、职称结构、学缘结构还待优化、高层次拔尖人才比较缺乏、众多中青年教师有待培养成熟等突出问题。并提出了政府应正确指导和支持;树立科学教育发展观,转变政府职能,加大对高等教育的经费投入,坚持完善和创新现行的有效措施;高校要进一步牢固树立"人才强校"战略,强化师资培养,加强人才引进,创新师资队伍建设模式;高校教师个人也要积极自我培养,通过加强教学工作实践与申报、开展科研课题等方式来达到锻炼和提高自身综合素质的的具体建议。

关键词：广东普通高校师资队伍建设

Transposition of maize trusposable element Ac in rice

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Rice Science 1991年第3期 6-6页

作者： zhang jingliu LOU Xiaomin CAI Ruizhu HUANG Ruixin HONG Mengmin Shanghai Institute of Plant Physiology,Academia Sinica,Shanghai 200032,China

To explore the possibility of using the maize(Zea mays L.)transposable element Activator(Ac)as a mutagen and gene tag in rice cells,we have intro-duced plasmid pKU3,a derivative of an Ac elementconstructed by Baker,in... 详细信息

关键词： NPT Transposition of maize trusposable element Ac in rice gene