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Liver metabolomics study reveals protective function of Phyllanthus urinaria against CCl_4-induced liver injury

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Chinese Journal of Natural Medicines 2017年第7期15卷 525-533页

作者： GUO Qing zhang Qian-Qian CHEN Jia-Qing zhang Wei QIU Hong-Cong zhang zun-jian LIU Bu-Ming XU Feng-Guo MOE Key Laboratory of Drug Quality Control and Pharmacovigilance China Pharmaceutical University Nanjing 210009 China State key laboratory of natural medicine China Pharmaceutical University Nanjing 210009 China State Key Laboratory for Quality Research in Chinese Medicines Macao University of Science & Technology Avenida Wai Long Taipa Macao China Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards Nanning 530022 China

Phyllanthus Urinaria L.(PUL) is a traditional Chinese medicine used to treat hepatic and renal disorders. However, the mechanism of its hepatoprotective action is not fully understood. In the present study, blood biochemical indexes and liver histopathological changes were used to estimate the extent of hepatic injury. GC/MS and LC/MS-based untargeted metabolomics were used in combination to characterize the potential biomarkers associated with the protective activity of PUL against CCl_4-induced liver injury in rats. PUL treatment could reverse the increase in ALT, AST and ALP induced by CCl_4 and attenuate the pathological changes in rat liver. Significant changes in liver metabolic profiling were observed in PUL-treated group compared with liver injury model group. Seventeen biomarkers related to the hepatoprotective effects of PUL against CCl_4-induced liver injury were screened out using nonparametric test and Pearson's correlation analysis(OPLS-DA). The results suggested that the potential hepatoprotective effects of PUL in attenuating CCl_4-induced hepatotoxicity could be partially attributed to regulating L-carnitine, taurocholic acid, and amino acids metabolism, which may become promising targets for treatment of liver toxicity. In conclusion, this study provides new insights into the mechanism of the hepatoprotection of Phyllanthus Urinaria.

关键词： Metabolomics Hepatic protection Phyllanthus Urinaria Carbon tetrachloride

Combination of LC/MS and GC/MS based metabolomics to study the hepatotoxic effect of realgar nanoparticles in rats

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Chinese Journal of Natural Medicines 2017年第9期15卷 684-694页

作者： zhang Mo-Han CHEN Jia-Qing GUO Hui-Min LI Rui-Ting GAO Yi-Qiao TIAN Yuan zhang zun-jian HUANG Yin Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education) China Pharmaceutical UniversityNanjing 210009 China State Key Laboratory of Natural Medicine China Pharmaceutical University Nanjing 210009 China

Realgar nanoparticles(NPs) are increasingly used as therapeutic agents for their enhanced anti-proliferation effect and cytotoxicity on cancer cells. However, the alteration of particle size may enhance biological reactivity as well as toxicity. A LC/MS and GC/MS based metabolomics approach was employed to explore the mechanism of realgar NPs-induced hepatotoxicity and identify potential biomarkers. Male Sprague-Dawley rats were administrated intragastrically with realgar or realgar NPs at a dose of 1.0 g·kg^(-1)·d^(-1) for 28 days and toxic effects of realgar NPs on liver tissues were examined by biochemical indicator analysis and histopathologic examination. Increased levels of serum enzymes and high hepatic steatosis were discovered in the realgar NPs treated group. Multivariate data analysis revealed that rats with realgar NPs-induced hepatotoxicity could be distinctively differentiated from the animals in the control and realgar treated groups. In addition, 21 and 32 endogenous metabolites were apparently changed in the serum and live extracts, respectively. Realgar NPs might induce free fatty acid and triglyceride accumulation, resulting in hepatotoxicity. In conclusion, the present study represents the first comprehensive LC/MS-and GC/MS-based metabolomics analysis of realgar NPs-induced hepatotoxicity, which may help further research of nanotoxicity.

关键词： Realgar nanoparticles Metabolomics Hepatotoxicity Liver extracts

Identification and comparative analysis of the major chemical constituents in the extracts of single Fuzi herb and FuziGancao herb-pair by UFLC-IT-TOF/MS

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Chinese Journal of Natural Medicines 2014年第7期12卷 542-553页

作者： YANG Yan YIN Xin-Juan GUO Hui-Min WANG Ru-Lin SONG Rui TIAN Yuan zhang zun-jian Key Laboratory of Drug Quality Control and Pharmacovigilance China Pharmaceutical UniversityMinistry of Education State Key Laboratory of Natural Medicines China Pharmaceutical University

AIM: The aim of this work was to establish a specific and sensitive method to comprehensively investigate and compare chemical constituents of Fuzi-Gancao herb pair(FG), consisting of Aconitum carmichaelii Debeaux(Fuzi, Chinese) and Roast Radix Glycyrrhizae(Glycyrrhiza glabra L., Gancao, in Chinese) and Fuzi alone to explore the underlying interaction mechanism of FG. METHOD: An ultra-fast liquid chromatography-ion trap/time-of-flight mass spectrometry(UFLC/MS-IT-TOF) method using diazepam as internal standard was developed for the identification and semi-quantitative analysis of the phytochemical constituents of Fuzi and FG. Chromatographic separation was achieved on a UFLC column using a gradient program with 40 mmol?L-1 ammonium acetate and acetonitrile as the mobile phase. RESULTS: Fifty-one of the sixty compounds, including forty-five C19-diterpenoid alkaloids and six C20-diterpenoid alkaloids were tentatively identified in the extracts of Fuzi and FG through accurate mass measurements and fragmentation patterns. Comparing the contents of these alkaloids in these two extracts, it was found that the diester-diterpenoid alkaloids(DDAs) and the alkylolamine-diterpenoid alkaloids(ADAs) were increased, while the monoester-diterpenoid alkaloids(MDAs) were decreased in the extracts of FG. CONCLUSION: This work provided comprehensive information for the quality control of Fuzi preparations, and the further investigation on the compatibility mechanisms of FG.

关键词： Aconitum carmichaelii Radix Rhizoma Glycyrrhizae Diterpenoid alkaloids UFLC-IT-TOF/MS

Strong evidence for latitudinal diversity gradient in mosses across the world

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Plant Diversity 2024年第4期46卷 537-541页

作者： Hong Qian zun Dai jian Wang Research and Collections Center Illinois State Museum1011 East Ash StreetSpringfieldIL 62703USA Bryology Laboratory School of Life SciencesEast China Normal UniversityShanghai 200241China Shanghai Institute of Eco-Chongming(SIEC) 3663 Northern Zhongshan RoadShanghai 200062China

Species richness generally decreases with increasing latitude,a biodiversity gradient that has long been considered as one of the few laws in *** latitudinal diversity gradient has been observed in many major groups of *** plants,the latitudinal diversity gradient has been observed in vascular plants,angiosperms,ferns,and ***,a conspicuous latitudinal diversity gradient in mosses at a global or continental scale has not been observed until ***,we analyze a comprehensive data set including moss species in each band of 20° in latitude *** results show that moss species richness decreases strongly with increasing latitude,regardless of whether the globe is considered as a whole or different longitudinal segments(e.g.,Old World versus New World) are considered *** result holds when variation in area size among latitudinal bands is taken into ***'s correlation coefficient between latitude and species richness is-0.99 for both the Northern and Southern *** bryophytes are an extant lineage of early land plants and because mosses not only include most of extant species of bryophytes but also are important constituents of most terrestrial ecosystems,understanding geographic patterns of mosses is particularly important The finding of our study fills a critical knowledge gap.

关键词： Bryophyte Latitudinal diversity gradient Moss Species density Species diversity

Sparse Modal Decomposition Method Addressing Underdetermined Vortex-Induced Vibration Reconstruction Problem for Marine Risers

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China Ocean Engineering 2024年第2期38卷 285-296页

作者： DU zun-feng ZHU Hai-ming YU jian-xing State Key Laboratory of Hydraulic Engineering Intelligent Construction and Operation School of Civil EngineeringTianjin UniversityTianjin 300350China

When investigating the vortex-induced vibration(VIV)of marine risers,extrapolating the dynamic response on the entire length based on limited sensor measurements is a crucial step in both laboratory experiments and fatigue monitoring of real *** problem is conventionally solved using the modal decomposition method,based on the principle that the response can be approximated by a weighted sum of limited vibration ***,the method is not valid when the problem is underdetermined,i.e.,the number of unknown mode weights is more than the number of known *** study proposed a sparse modal decomposition method based on the compressed sensing theory and the Compressive Sampling Matching Pursuit(Co Sa MP)algorithm,exploiting the sparsity of VIV in the modal *** the validation study based on high-order VIV experiment data,the proposed method successfully reconstructed the response using only seven acceleration measurements when the conventional methods failed.A primary advantage of the proposed method is that it offers a completely data-driven approach for the underdetermined VIV reconstruction problem,which is more favorable than existing model-dependent solutions for many practical applications such as riser structural health monitoring.

关键词： motion reconstruction vortex-induced vibration(VIV) marine riser modal decomposition method compressed sensing

A four-component combination derived from Huang-Qin Decoction significantly enhances anticancer activity of irinotecan

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Chinese Journal of Natural Medicines 2021年第5期19卷 364-375页

作者： XU Dou-Dou HOU Xiao-Ying WANG Ou WANG Di LI Dan-Ting QIN Si-Yuan LV Bo DAI Xiao-Min zhang zun-jian WAN jian-Bo XU Feng-Guo Key Laboratory of Drug Quality Control and Pharmacovigilance(Ministry of Education) State Key Laboratory of Natural MedicineChina Pharmaceutical UniversityNanjing 210009China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacaoChina

Huang-Qin Decoction(HQD)is a classic prescription for diarrhea in Chinese medicine *** studies have demonstrated that HQD and its modified formulation PHY906 could ameliorate irinotecan(CPT-11)induced gastrointestinal(GI)toxicity and enhance its anticancer therapeutic ***,which constituents in HQD are effective is still unclear so *** study aims to screen out the key bioactive components combination from HQD that could enhance the anticancer effect of ***,the potential bioactive constituents were obtained through system pharmacology *** the bioactivity of each constituent was investigated synthetically from the aspects of NCM460 cell migration,TNF-αrelease of THP-1-derived macrophage and MTT assay in HCT116 *** contribution of each constituent in HQD was evaluated using the bioactive index Ei,which taken the content and bioactivity into comprehensive *** then,the most contributing constituents were selected out to form a keycomponent *** last,the bioefficacy of the key-component combination was validated in vitro and in *** a result,a key-component combination(HB4)consisting of four compounds baicalin,baicalein,glycyrrhizic acid and wogonin was screened *** vitro assessment indicated that HB4 could enhance the effect of CPT-11 on inhibiting cell proliferation and inducing apoptosis in ***,the in vivo study confirmed that HB4 and HQD have similar pharmacological activity and could both enhance the antitumor effect of CPT-11 in HCT116 xenograft ***,HB4 could also reduce the CPT-11 induced GI toxicity.

关键词： Active components Bioactivity contribution Huang-Qin Decoction Irinotecan Toxicity attenuating and efficacy enhancing

Iron–doped bismuth oxybromides as visible-light-responsive Fenton catalysts for the degradation of atrazine in aqueous phases

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Journal of Environmental Sciences 2024年第3期137卷 321-332页

作者： Yong-Li Cai Yu-Hang Xu Ji-zun Xiang Zhi-Qiang zhang Qiu-Xiang He Ya-Feng Li jian Lü Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation College of Resources and EnvironmentFujian Agriculture and Forestry UniversityFuzhou 350002China State Key Laboratory of Photocatalysis on Energy and Environment Fuzhou UniversityFuzhou 350116China

Pesticides and its degradation products,being well–known residues in soil,have recently been detected in many water bodies as pollutants of emerging concerns,and thus there is a contemporary demand to develop viable and cost–effective techniques for the removal of related organic pollutants in aqueous ***,a visible-light-responsive Fenton system was constructed with iron–doped bismuth oxybromides(Fe–BiOBr)as the *** the advantage of sustainable Fe(Ⅲ)/Fe(Ⅱ)conversion and optimized H_(2)O_(2)utilization,the optimal Fe–BiOBr–2 catalyst showed an excellent atrazine removal efficiency of 97.61%in 120 min,which is superior than the traditional homogeneous Fenton and the majority of heterogeneous processes documented in the *** this photo–Fenton system,hydroxyl(·OH)and superoxide(·O_(2)^(-))radicals were dominant active species contributed to the oxidative degradation of *** to the production of various active radicals,five degradation pathways were proposed based on the identification of intermediates and degradation ***,this work not only demonstrates a fundamental insight into creating highly efficient and atom economic photo-Fenton systems,but also provides a complementary strategy for the treatment of organic pollutants in water.

关键词： Bismuth oxybromide Photo–Fenton Atrazine Catalytic degradation

复方氯雷他定缓释微丸胶囊的药代动力学与相对生物利用度

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中国药科大学学报 2006年第1期37卷 49-53页

作者：田媛张尊建中国药科大学分析测试中心南京210009

目的:研究复方氯雷他定缓释微丸胶囊中氯雷他定和硫酸伪麻黄碱在中国健康志愿者体内的药代动力学行为及相对生物利用度。方法:20名健康受试者单剂量及多剂量交叉服用受试制剂和参比制剂后,采用LC-MS法分别测定氯雷他定和硫酸伪麻黄碱在... 详细信息

目的:研究复方氯雷他定缓释微丸胶囊中氯雷他定和硫酸伪麻黄碱在中国健康志愿者体内的药代动力学行为及相对生物利用度。方法:20名健康受试者单剂量及多剂量交叉服用受试制剂和参比制剂后,采用LC-MS法分别测定氯雷他定和硫酸伪麻黄碱在血浆中的浓度,估算其药代动力学参数。结果:试验胶囊中的缓释成分硫酸伪麻黄碱与参比片中硫酸伪麻黄碱相比,cmax降低,tmax延长,波动度(DF)较小。单剂量时氯雷他定的相对生物利用度为(97.6±17.4)%,硫酸伪麻黄碱为(98.8±15.5)%。多剂量时氯雷他定的相对生物利用度为(94.6±16.0)%,硫酸伪麻黄碱为(94.4±13.9)%。结论:两成分测定结果经方差分析及双单侧t检验,证明两制剂生物等效。

关键词：氯雷他定硫酸伪麻黄碱高效液相色谱-质谱法药代动力学生物利用度

Recent progress in carbene-catalyzed fluoroalkylation

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Science China Chemistry 2024年第8期67卷 2448-2460页

作者： Bei zhang Xuan zhang jian Wang jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control Collaborative Innovation Center of Atmospheric Environment and Equipment TechnologySchool of Environmental Science and EngineeringNanjing University of Information Science and TechnologyNanjing 210044China School of Pharmaceutical Sciences Tsinghua UniversityBeijing 100084China

The fluoroalkyl fragment plays a vital role in various fields such as pharmaceuticals and materials science, and has a significant impact on human life not only on the present but also in the future. The insertion of fluoroalkyl groups into the chemical skeletons can cause considerable alternations in the chemical, physical and biological properties of molecules, which has attracted numerous scientists from different fields. This review focuses on N-heterocyclic carbene(NHC)-catalyzed fluoroalkylation reactions, providing a detailed analysis and summary of the achievements in the past two decades. Many successful examples clearly indicate that NHCs can catalyze the tranformation of fluoroalkylation reagents with various electronic characteristics, whether electrophilic, nucleophilic, or radical precursors. Herein, the fluoroalkylation reactions are classified based on different catalytic modes of NHCs: Lewis base catalysis, Umploung catalysis, radical catalysis and others. Meanwhile, by refining and systematically studying the contribution of NHC catalysis in fluoroalkylation reactions, this review is expected to provide valuable insights and enlightenment for related fields.

关键词： N-heterocyclic carbene fluoroalkylation radical catalysis