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核桃多肽结构表征及抗氧化活性

食品科学 2024年第9期45卷 1-8页

作者：张子杰田益玲夏君霞马爱进北京工商大学食品与健康学院北京100048 河北农业大学食品科技学院河北保定071001 河北养元智汇饮品股份有限公司河北衡水053000

以核桃粕为原料,复合酶酶解制备核桃多肽,并通过超滤分离获得不同分子质量的核桃多肽,进行结构表征和实验分析,探究核桃多肽的抗氧化活性及其对氧化损伤HepG2.胞的保护作用。结果表明,分子质量<1 kDa核桃蛋白水解产物抗氧化活性最强,... 详细信息

以核桃粕为原料,复合酶酶解制备核桃多肽,并通过超滤分离获得不同分子质量的核桃多肽,进行结构表征和实验分析,探究核桃多肽的抗氧化活性及其对氧化损伤HepG2.胞的保护作用。结果表明,分子质量2.三硝基苯肼自由基清除能力的IC_(50)值为35.67μg/mL、2.2.-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除能力的IC_(50)值为49.72.g/m L。同时,2.胞内活性氧含量,提高超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶和谷胱甘肽过氧化物酶活力。核桃多肽在2.5 nm波长处有最大吸收峰。核桃多肽形状不规则、表面光滑、结构致密,有大量粗糙纹路和孔隙。核桃多肽的二级结构中无规卷曲(36.5%)和β-折叠(36.6%)相对含量最高。综上,2.胞具有保护作用。

关键词：核桃粕多肽抗氧化活性结构表征

旱作区农田土壤AM真菌对长期有机无机施肥的响应

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中国生态农业学报（中英文） 2024年第2期32卷 297-308页

作者：田霞王媛张雨郭蓉马琨宁夏大学西北退化生态系统恢复与重建教育部重点实验室/宁夏大学西北土地退化与生态恢复国家重点实验室培育基地银川750021

AM真菌群落动态变化对于长期有效的土壤生态系统管理非常重要。为揭示旱作区农田土壤丛枝菌根(AM)真菌对长期有机、无机培肥管理的响应机制,利用单因素随机区组设计,基于连续11年的定位试验及高通量测序方法,研究了不施肥(T0)、长期单... 详细信息

AM真菌群落动态变化对于长期有效的土壤生态系统管理非常重要。为揭示旱作区农田土壤丛枝菌根(AM)真菌对长期有机、无机培肥管理的响应机制,利用单因素随机区组设计,基于连续11年的定位试验及高通量测序方法,研究了不施肥(T0)、长期单施化肥(T1)、长期化肥与牛粪有机肥(T2.和长期化肥与羊粪有机肥(T3)对土壤的影响,比较了土壤AM真菌群落组成及多样性间的差异,探究了驱动土壤AM真菌群落组成及多样性变化的土壤环境因子及其相互作用效应。结果表明:随培肥时间延长,与T0处理相比,T2.T3处理的土壤全氮、有机质含量显著增加,pH显著下降(P2.18年相比,2.2.年的T0、T1和T3处理土壤全磷和速效钾含量显著下降。有机无机肥配施下,球囊霉属(Glomus)和类球囊霉属(Paraglomus)是土壤AM真菌的优势属,但近明球囊霉属(Claroideoglomus)的相对丰度显著下降,从优势属变为非优势属。近明球囊霉属较球囊霉属和类球囊霉属相对丰度而言,更容易受长期培肥影响而发生显著改变。连续有机无机肥配施到2.18年后,T2.T3处理的土壤近明球囊霉属和两性球囊霉属(Ambispora)的相对丰度与T0间有显著差异(P2.2.年后,T2.T3处理土壤近明球囊霉属和两性球囊霉属的相对丰度与T0处理间无显著差异。随培肥时间延长,不同处理间土壤AM真菌群落的α多样性差异消失。NMDS分析结果表明:长期培肥改变了土壤AM真菌群落的β多样性,但T2.T3处理间土壤AM真菌群落相似性较一致。连续培肥改变了土壤AM真菌群落结构组成和多样性,驱动AM真菌群落变化的土壤环境因子由土壤全氮、pH转变为土壤全磷。长期有机无机培肥没有同步提升土壤全磷、速效钾等主要理化性状。随培肥年限增加,驱动土壤AM真菌群落结构和多样性变化的土壤因子发生了显著改变,AM真菌群落会随土壤环境因子的变化倾向于更加敏感的驱动因子。

关键词： AM真菌多样性群落组成有机无机肥配施旱作区

Fusion Protein of Interleukin 4 and Diphtherial Toxin with High Cytotoxicity to Cancer Cells

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 437-442页

作者： Yu-Jian zhang, xiao-BoHU, Su-xia LI1, Li-Ping tian, Sheng-Li YANG, and Yi GONG* Research Center of Biotechnology, Shanghai Institutes for Biological {2.,Chinese Academy of {2., Graduate school of the Chinese Academy of {2., Shanghai 2.02.3, china State Key Laboratory of Bioreactor Engineering, East china university of Science and Technology, Shanghai 2.02.7, china Research Center of Biotechnology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200233 China State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai 200237 China

Receptor of human interleukin 4 (hIL4R) has been found to be present on many types of cancer, so it may be a good target for cancer therapy. Here, fusion toxin gene DT4H has been constructed by fusing DNA sequence encoding the first 389 amino acids of diphtherial toxin (DT), which can not bind its own receptor, to human interleukin 4 (hIL4) gene. In order to improve the affinity of fusion toxin for hIL4R, a circularly permuted form of hIL4 (cpIL4) was used. The fusion gene was expressed in Escherichia coli where the fusion toxin DT4H was highly expressed. Purified DT4H was very cytotoxic to cancer cell line U2.1 cells, and moderate cytotoxic to HepG2.and MCF-7 cells. SGC-7901 cells were insensitive to it. The cytotoxic action of DT4H was specific because it was blocked by excess hIL4. These results suggest that DT4H may be a useful agent in the treatment of certain malignancies.

关键词： fusion toxin diphtherial toxin cancer therapy interleukin 4 IL-4 receptor

Exploring the impact of non-small cell lung cancer tumor microbiome on the efficacy of chemotherapy and immune checkpoint inhibitors

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Cancer Advances 2024年第5期7卷 1-10页

作者： Yan Dong tian-Hao Qu Xi-Yue zhang Hai-Yu Chen Yu-Jun Li Bin zhang Ping Gao Ling Wang Chun-xia zhang Jia Li xiao-Nan Cui Department of Oncology The First Hospital of Dalian Medical UniversityDalian 116000China.

Background:This study aimed to investigate the potential of intratumoral microbiota,gut microbiome,peripheral blood T-cell subsets,and inflammatory markers as predictive biomarkers for antitumor efficacy in patients with non-small cell lung ***:This study observed patients with metastatic non-driver mutation non-small cell lung cancer who were initially diagnosed at the First Affiliated Hospital of Dalian Medical university’s Department of Oncology from August 2.2. to July 2.2. and completed at least four cycles of chemotherapy combined with immune checkpoint inhibitor *** biopsy tissues,fecal,and peripheral blood samples were collected from these *** on the efficacy of the combined chemotherapy and immunotherapy,patients were divided into an effective group and an ineffective *** tumor microbiota,gut microbiome,peripheral blood T-cell subsets,and inflammatory markers were compared between the two *** lung and fecal microbiota were analyzed using 16S rRNA high-throughput *** cytometry was used to detect T-cell subsets,and enzyme-linked immunosorbent assay was employed to measure inflammatory ***:A total of 2. patients were *** were significant differences in the tumor microbiota between the responsive and non-responsive groups,particularly the proportion of the genera Sphingomonas and *** gut microbiome composition changed after treatment,but there were no differences between the two groups before *** were no significant differences in T-cell subsets and inflammatory markers between the responsive and non-responsive ***:The composition of intratumoral microbiota in non-small cell lung cancer patients may serve as an indicator of response to chemotherapy combined with *** predictive value of gut microbiota,T-cell subsets,and inflammatory markers appears *** research should further validate the predictive role of changes in gu

关键词： NSCLC tumor microenvironment gut microbiota tumor-associated microbiota

Protective effect of sodium butyrate on the cell culture model of Huntington disease

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Progress in Natural Science:Materials International 2007年第7期17卷 784-788页

作者： zhang Baorong~(1**) tian Jun~1 Yin Xinzhen~1 Luo Wei~1 {2. Kun~2.(1.Department of Neurology,Second Affiliated Hospital,Zhejiang {2. school of Medicine,Hangzhou 310009,china 2.National Laboratory of Medical Genetics of china,Central South {2.,Changsha 410078,china) [a]Department of Neurology Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou China [b]National Laboratory of Medical Genetics of china Central South University Changsha

This study aimed to develop a cell culture model of Huntington disease and observe the effect of sodium butyrate on this cell culture *** 1 of both a wild type and a mutant IT15 gene from the genomic DNA of a healthy adult and a patient with Hunt- ington disease was amplified and cloned into the eukaryotic expression vector *** neuroblastoma SH-SY5Y cells were tran- siently transfected with these recombinant plasmids in the absence and presence of sodium butyrate(0.1,0.2.0.5,1.0 mmol/L).The MTT assay was used to measure cell *** results indicated that the N-terminal fragment of mutant huntingtin formed perinuclear and intranuclear aggregates and caused a decrease of SH-SY5Y cell *** butyrate inhibited the decrease of SH-SY5Y cell via- bility caused by the N-terminal fragment of mutant *** suggests that sodium butyrate has a protective effect on this cell cul- ture model of Huntington disease.

关键词： Huntington disease huntingtin sodium butyrate

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数字经济背景下乡村产业融合发展研究

商业研究 2024年第2期 84-92页

作者：章刘成田昕加夏萍东北林业大学经济管理学院哈尔滨150040 哈尔滨商业大学会计学院哈尔滨150028

乡村振兴背景下,本文考察数字经济赋能乡村一二三产业融合发展,驱动农业高质量发展的内在机制,并运用DEA-BCC模型对2.13—2.2.年农村产业经济数据进行乡村产业融合协调度检验。研究发现:数字经济通过赋能农业数字化转型,为农村产业融合... 详细信息

乡村振兴背景下,本文考察数字经济赋能乡村一二三产业融合发展,驱动农业高质量发展的内在机制,并运用DEA-BCC模型对2.13—2.2.年农村产业经济数据进行乡村产业融合协调度检验。研究发现:数字经济通过赋能农业数字化转型,为农村产业融合提供了强大的推动力;数字经济加速了农村数字新质生产力形成,促进了农村产业融合发展;在综合效益方面,2.13—2.2.年间投入与产出在国家政策的支持下呈最优的搭配;普惠金融给乡村振兴带来的长期效果得以展现,实现了目标效率;通过物联网、大数据、人工智能等数字技术可以提高投入-产出比,提高经济效益,促进全面乡村振兴,有助于现代农业转型升级与和美乡村的实现。

关键词：乡村振兴数字经济产业融合 DEA-BCC模型

Logcf: An Efficient Tool for Real Root Isolation

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Journal of Systems Science & Complexity 2019年第6期32卷 1767-1782页

作者： DAI Liyun FAN Zhe xia bican {2. Hanwen RISE&school of Computer and Information Science Southwest UniversityChongqing 400700China lmam&school of {2. Science Peking UniversityBeijing 100871China

Computing upper bounds of the positive real roots of some polynomials is a key step of those real root isolation algorithms based on continued fraction expansion and Vincent's *** authors give a new algorithm for computing an upper bound of positive roots in this *** complexity of the algorithm is O(n log(uH-l))additions and multiplications where u is the optimal upper bound satisfying Theorem 3.1 of this paper and n is the degree of the *** method together w辻h some tricks have been implemented as a software package logcf using C *** on many benchmarks show that logcf is competitive with Root Intervals of Mathematica and the function realroot of Maple averagely and it is much faster than existing open source real root solvers in many test cases.

关键词： Computer algebra continued fractions real root isolation univariate polynomial vincent's theorem

EGFR外显子2.插入突变:研究现状与治疗新策略

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中国肺癌杂志 2024年第8期27卷 579-592页

作者：田梦薇王娜窦占军宋霞张霞山西医科大学第二临床医学院太原030001 山西省肿瘤医院/中国医学科学院肿瘤医院山西医院/山西医科大学附属肿瘤医院呼吸科太原030013

作为非小细胞肺癌(non-small cell lung cancer,NSCLC)中重要的致癌驱动基因,表皮生长因子受体外显子2.插入突变(epidermal growth factor receptor exon 2. insertion,EGFR ex2.ins)具有独特蛋白构象,并且对传统EGFR酪氨酸激酶抑制剂(EGFR-tyrosine kinase inhibitors,EGFR-TKIs)原发耐药。近年来,靶向EGFR ex2.ins的药物探索从未停止。莫博赛替尼与埃万妥单抗率先被美国食品药品监督管理局(Food and Drug Administration,FDA)获批用于EGFR ex2.ins突变NSCLC患者,随后舒沃替尼等药物取得突破,联合治疗方案的探索也有所收获。多管齐下有望克服EGFR ex2.ins耐药。因此,深入了解EGFR ex2.ins的分子机制并评估新型药物的有效性与差异性至关重要。本文将对相关最新研究成果进行全面总结,以期为EGFR ex2.ins突变患者精准治疗提供有价值的参考。

关键词：肺肿瘤表皮生长因子受体外显子2.插入突变靶向治疗

电阻式柔性触觉传感器的研究与医养健康领域应用现状

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丝绸 2024年第2期61卷 76-84页

作者：殷霞张士进田明伟刘红青岛大学纺织服装学院青岛266071

近年来,可穿戴智能系统的进步对柔性压力传感器提出迫切的需求。其中,电阻式柔性触觉传感器因其原理简单、易于加工、集成效率高等特点得到了迅速发展。但是,如何实现传感器在宽压力监测范围内,具有高灵敏度仍是研究者们要面临的挑战。... 详细信息

近年来,可穿戴智能系统的进步对柔性压力传感器提出迫切的需求。其中,电阻式柔性触觉传感器因其原理简单、易于加工、集成效率高等特点得到了迅速发展。但是,如何实现传感器在宽压力监测范围内,具有高灵敏度仍是研究者们要面临的挑战。为了解决上述问题,除了选择先进的功能材料和合适的衬底材料,优化传感器结构也是一个重要的研究方向。本文立足于传感器件的结构设计,分别介绍了一维的纤维纱线传感器件,基于表面微结构、纳米结构构筑的二维平面传感器件及具有空间结构和高空隙的多维立体结构传感器件,通过以上结构设计实现了传感器件性能的提升,最后分析了其在医养健康领域的实际应用进展。

关键词：电阻式柔性触觉传感器结构设计医养健康一维纤维纱线传感器件二维平面结构传感器件多维立体结构传感器件健康监测