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Analysis of quality-related proteins in golden pompano(Trachinotus ovatus)fillets with modified atmosphere packaging under superchilling storage

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Food Science and Human Wellness 2024年第4期13卷 2253-2265页

作者： Chuang Pan xiaofan zhang Shengjun chen yong Xue Yanyan Wu Yueqi Wang di Wang South China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesKey Laboratory of Aquatic Product ProcessingMinistry of Agriculture and Rural AffairsNational R&D Centre for Aquatic Product ProcessingGuangzhou 510300China Co-Innovation Center of Jiangsu Marine Bio-industry Technology Jiangsu Ocean UniversityLianyungang 222005China Collaborative Innovation Center of Seafood Deep Processing Dalian Polytechnic UniversityDalian 116034China Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province Sanya Tropical Fisheries Research InstituteSanya 572018China College of Food Science and Engineering Ocean University of ChinaQingdao 266003China

here,we aimed to study the changes in proteome of golden pompano fillets during post-mortem *** mass tags(TMT)-labeled quantitative proteomic strategy was applied to investigate the relationships between protein changes and quality characteristics of modified atmosphere packaging(MAP)fillets during superchilling(-3°C)*** electron microscopy was used to show that the muscle histology microstructure of fillets was damaged to varying degrees,and low-field nuclear magnetic resonance was used to find that the immobilized water and free water in the muscle of fillets changed *** sulfhydryl content,TCA-soluble peptides and Ca2+-ATPase activity also showed that the fillet protein had a deterioration by oxidation and *** Fresh(FS),MAP,and air packaging(AP)groups were *** of 150 proteins were identified as differential abundant proteins(DAPs)in MAP/FS,while 209 DAPs were in AP/FS *** KEGG pathway analysis indicated that most DAPs were involved in binding proteins and protein *** analysis found that 52 DAPs were correlated with quality *** them,8 highly correlated DAPs are expected to be used as potential quality markers for protein oxidation and water-holding *** results provide a further understanding of the muscle deterioration mechanism of packaging golden pompano fillets during superchilling.

关键词： Tandem mass tags(TMT)proteomics Trachinotus ovatus Modified atmosphere packaging Superchilling storage Low-field nuclear magnetic resonance Protein deterioration

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Recurrence and survival after surgery for pancreatic cancer with or without acute pancreatitis

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World Journal of Gastroenterology 2019年第39期25卷 6006-6015页

作者： Qian feng cheng Li Sheng zhang chun-Lu Tan Gang Mai Xu-Bao Liu yong-Hua chen Department of Pancreatic Surgery West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Rehabilitation Medicine Center West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of hepatobiliary Surgery The First Affiliated Hospital of Xinxiang Medical UniversityXinxiang 453100Henan ProvinceChina Department of hepatobiliary Surgery Deyang People's HospitalDeyang 618000Sichuan ProvinceChina

BACKGROUND In pancreatic cancer,acute pancreatitis(AP)is a serious morbidity,but its negative effect on long-term outcomes remains to be *** To investigate the effects of AP on the tumor recurrence pattern of pancreatic ductal adenocarcinoma(PDAC)and tumor-specific *** The medical records of 219 patients with curative pancreatectomy for pancreatic cancer at the Pancreatic Surgery Center of West China Hospital from July 2012 to December 2016 were analyzed *** severity of acute pancreatitis was classified according to the Atlanta classification of *** patient demographics and tumor characteristics were *** recurrence was defined as a relapse within 12 mo after *** and disease-free survival and recurrence patterns were *** acute pancreatitis was excluded because its negative effects can be *** Early recurrence in AP group was significantly higher than in non-AP group(71.4%vs 41.2%;P=0.009).Multivariate analysis of postoperative early recurrence showed that moderate or severe AP was an independent risk factor for an early recurrence[odds ratio(OR):4.13;95%confidence interval(CI):1.41-12.10;P=0.01].The median time to recurrence was shorter in patients with AP than in those without(8.4 vs 12.8 mo;P=0.003).Multivariate analysis identified AP as an independent prognostic factor for overall survival[relative risk(RR):2.35;95%CI:1.45-3.83]and disease-free survival(RR:2.24;95%CI:1.31-3.85)in patients with *** Patients with moderate or severe acute pancreatitis developed recurrences earlier than those *** or severe AP is associated with shorter overall and disease-free survival of patients with PDAC.

关键词： Acute pancreatitis Pancreatic ductal adenocarcinoma Recurrence Prognosis Overall survival disease-free survival

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Mechanical properties and surface characteristics of SiC fibers irradiated by swift heavy ions

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Nuclear Science and Techniques 2022年第9期33卷 37-47页

作者： Li-Qing zhang Chong-Hong zhang chen-chun Hao Guo-Dong Hu Zheng-Dong feng yong-Jian Tang Wen-Kun Zhu Jian-Yang Li Qing Huang Southwest University of Science and Technology Mianyang 621010China Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesNingbo 315201China

SiC fibers were irradiated by 414.4-MeV^(112)Sn^(27.3+)ions to different fluences(5.0×10^(12),6.0×10^(13),1.6×10^(14),and 1.92×10^(15)ions/cm^(2)).^(112)Sn^(27.3+)deposited its energy mainly via electron energy loss and passed through the SiC ***,the mechanical properties and surface characteristics of fibers were studied using a specific single filament tensile test and field emission scanning electron *** revealed that the carbon concentration on the fiber surface increased while the silicon concentration ***,the addition of oxygen was found to correlate with an increase in ion ***,the fiber surface morphology of the least fluence(5.0×10^(12)ions/cm^(2))irradiated specimen displayed no obvious changes and its diameter was slightly *** successive increases of ion fluence,large grains/bubbles on the fiber surface first appeared and then disappeared,and the diameter of fibers evidently ***,at the highest fluence(1.92×10^(15)Sn ions/cm^(2))irradiated specimen,some fibers were brittle *** a result,the mean tensile strength and the average elastic modulus of the fibers generally decreased with respect to the ion *** degradation mechanisms of mechanical properties of SiC fibers under irradiation are discussed in detail.

关键词： SiC fibers Swift-heavy-ion irradiation FE SEM Tensile test

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Case History of the disastrous Debris Flows of Tianmo Watershed in Bomi County, Tibet, China: Some Mitigation Suggestions

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Journal of Mountain Science 2014年第5期11卷 1253-1265页

作者： GE yong-gang CUI Peng SU feng-huan zhang Jian-qiang chen xing-zhang Key Laboratory of Mountain Hazards and Earth Surface Processes / Institute of Mountain Hazards and Environment Chinese Academy of Sciences School of Environment and Resources Southwest University of Sciences and Technology

Debris flows and landslides, extensively developing and frequently occurring along Parlung Zangbo, seriously damage the Highway from Sichuan to Tiebt(G318) at Bomi County. The disastrous debris flows of the Tianmo Watershed on Sept. 4, 2007, July 25, 2010 and Sept. 4, 2010, blocked Parlung Zangbo River and produced dammed lakes, whose outburst flow made 50 m high terrace collapse at the opposite bank due to intense scouring on the foot of the terrace. As a result, the traffic was interrupted for 16 days in 2010 because that 900 m highway base was destructed and 430 m ruined. These debris flows were initiated by the glacial melting which was induced by continuous higher temperature and the following intensive rainfall, and expanded by moraines along channels and then blocked Parlung Zangbo. At the outlet of watershed,the density, velocity and peak discharge of debris flow was 2.06 t/m3, 12.7 m/s and 3334 m3/s, respectively. When the discharge at the outlet and the deposition volume into river exceeds 2125 m3/s and 126×103 m3, respectively, debris flow will completely blocked Parlung Zangbo. Moreover,if the shear stress of river flow on the foot of terrace and the inclination angel of terrace overruns 0. 377 N/m2 and 26°, respectively, the unconsolidated terrace will be eroded by outburst flow and collapse. It was strongly recommended for mitigation that identify and evade disastrous debris flows, reduce the junction angel of channels between river and watershed, build protecting wall for highway base and keep appropriate distance between highway and the edge of unconsolidated terrace.

关键词： Debris Flow Characteristics Hazard Chain Formation Condition Tianmo Watershed

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Experimental evidence of the thickness- and electric-field-dependent topological phase transitions intopological crystalline insulator SnTe（111） thin films

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Nano Research 2018年第11期11卷 6045-6050页

作者： Yan Gong Kejing Zhu Zhe Li Yunyi Zang xiao feng ding zhang Canli Song Lili Wang Wei Li xi chen Xu-Cun Ma Qi-Kun Xue yong Xu Ke he State Key Laboratory of Low-dimensional Quantum Physics Department of Physics Tsinghua University Beijing 100084 China Collaborative Innovation Center of Quantum Matter 8eijing 100084 China

Using in situ angle-resolved photoemission spectroscopy, we systematically studied molecular-beam-epitaxy-grown topological crystalline insulator SnTe(111) thin films with varied thicknesses and substrate conditions. An oscillation in the band gap size with an increase in thickness was observed to depend on the electric field perpendicular to the films. The observations are consistent with the theoretically predicted thickness- and electric-field-dependent topological phase transitions between the normal insulator and the quantum spin Hall insulator phases in SnTe(111) films and demonstrate them to be an excellent and electrically tunable quantum spin Hall system.

关键词： topological crystalline insulator SnTe quantum spin Hall effect field effect

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Room-temperature test system for 162.5 MHz high-power couplers

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Nuclear Science and Techniques 2019年第1期30卷 35-41页

作者： Long chen Shen-Hu zhang yong-Ming Li Ruo-Xu Wang Tiancai Jiang Lei Yang chun-Long Li An-Dong Wu Shi-chun Huang feng Pan xin-Meng Liu Yuan he University of Chinese Academy of Sciences Institute of Modern Physics Chinese Academy of Sciences

Fundamental power couplers are crucial components for feeding radio frequency power to accelerating cavities. Couplers must be tested and conditioned on a room-temperature test stand to evaluate and potentially improve their performance before being installed in an accelerating cavity. A new test system has been designed and is under construction at the institute of modern *** this test system, multiple conditioning modes, including the pulse mode, CW mode, and amplitude-sweeping mode, have been embedded in the low-level radio frequency system of the test stand. All of these conditioning modes can be run manually or automatically. In addition, a novel test cavity is proposed and has been designed, which facilitates non-contact conditioning and a multi-purpose test stand.

关键词： Couplers conditioning Test stand LLRF control system Test cavity

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Failure Characteristics of Rock-like Mortar Specimens with Arc-shaped Flaws under Freezing-thaw Cycles and Uniaxial Compression

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Journal of Wuhan University of Technology(Materials Science) 2024年第6期39卷 1461-1473页

作者： LI yong LI Kunpeng zhang Shaowang YIN Futong WANG chen DAI feng WANG Kai Institute of Geotechnical and Underground Engineering Shandong UniversityJinan 250061China School of Qilu Transportation Shandong UniversityJinan 250002China School of Civil Engineering Shandong UniversityJinan 250061China Shandong Hispeed Group Jinan 250014China

To investigate the freeze-thaw(F-T)damages and failure characteristics of rock mass with arc-shaped joints in cold regions,three types of cement mortar specimens with different central angles and prefabricated arc-shaped flaws are subjected to uniaxial compressive tests under different F-T *** observations show that the uniaxial compressive strength of specimens are significantly influenced by F-T cycles and their failure modes are mainly affected by the central angleαof the prefabricated *** the specimens with a central angle of 60°,the specimens with a central angle of 120°and 180°have greater curvature of flaws,so tensile cracks occur in the arc-top area of their prefabricated *** to experimental images observed by environmental scanning electron microscope(ESEM),as the number of F-T cycles increases,the deterioration effect of the specimen becomes more obvious,which is specifically reflected in the increase of the mass loss,peak stress loss,and damage variables as a power function,and the peak strain decreases as a quadratic *** to numerical results using two-dimensional particle flow code(PFC2D),it is found that F-T cycles cause more damage to the specimen in the early stages than in the later *** area of the concentrated compressive stress zone in the middle is decreased due to the increased number of F-T cycles,while the area of the surrounding tensile-shear stress zone is *** models appear different failure modes due to the release of concentrated stress in different tensile-shear zones.

关键词： cement mortar material arc-shaped flaws freeze-thaw cycle failure pattern PFC2D

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Contribution and underlying mechanisms of CXCR4 overexpression in patients with systemic lupus erythematosus

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Cellular & Molecular Immunology 2017年第10期14卷 842-849页

作者： Li-dan Zhao di Liang xiang-ni Wu Yang Li Jing-wen Niu chen Zhou Li Wang Hua chen Wen-jie Zheng Yun-yun Fei Fu-lin Tang yong-zhe Li feng-chun zhang Wei he Xue-tao Cao Xuan zhang Department of Rheumatology and Clinical Immunology Peking Union Medical College HospitalClinical Immunology CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeThe Ministry of Education Key LaboratoryBeijing 100730China Department of Immunology School of Basic MedicinePeking Union Medical Collegeand Institute of Basic Medical SciencesChinese Academy of Medical SciencesBeijing 100005China

Aberrant expression of CXCR4 has been indicated to play a role in the pathogenesis of systemic lupus erythematosus(SLE),but the mechanism of CXCR4 dysregulation in SLE is *** study is aimed to explore the clinical significance and possible mechanisms of abnormal CXCR4 expression on B cells from patients with untreated *** of CXCR4 on peripheral B cells was determined by flow cytometry and western *** isolated B cells were cultured with exogenous interleukin 21(IL-21)in the presence or absence of CD40 ligand(CD40L)plus anti-IgM antibody(aIgM),and changes in CXCR4 expression were *** of phosphatidylinositol 3 kinase(PI3K)/Akt and Janus kinase/Signal transducer and activator of transcription(JAK/STAT)signaling pathways was assessed by adding blocking agents Ly294002 and *** CD63 is reported to mediate endosomal recruitment of CXCR4 and BCL6 is capable of silencing CD63 gene transcription,we also measured BCL6 and CD63 gene transcription with real-time *** was shown that CXCR4 expression on B cells was significantly upregulated in SLE patients,especially in those with lupus nephritis,and was positively correlated with SLE disease Activity Index scores and negatively with the serum complement 3 levels(Po0.05).Downregulation of CXCR4 by IL-21 was *** contrast,a similar effect of aIgM plus CD40L in downregulating CXCR4 expression was defective in SLE patients but was restored by co-stimulation with IL-21 in *** Ly294002 and AG490 promoted downregulation of surface CXCR4 expression on B cells from SLE patients(P=0.078 and P=0.064).Furthermore,B cells from SLE patients exhibited diminished CD63 mRNA and enhanced BCL6 mRNA expression(both Po0.05).To sum up,CXCR4 was overexpressed on SLE B cells,positively correlating with disease activity and kidney *** of the PI3K/Akt and JAK/STAT pathways as well as defective CD63 synthesis may contribute to CXCR4 dysregulation in SLE.

关键词： CXCR4 IL-21 signaling pathway systemic lupus erythematosus

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Alanine aminotransferase as a risk marker for new-onset metabolic dysfunction-associated fatty liver disease

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World Journal of Gastroenterology 2024年第25期30卷 3132-3139页

作者： di Wang Bing-Yan Zhou Lei xiang Xu-yong chen Jie-xiong feng Department of Pediatric Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina

In this editorial,we comment on the article by chen et *** dysfunction-associated fatty liver disease(MAFLD)is a global public health burden whose incidence has risen concurrently with overweight and *** its detri-mental health impact,early identification of at-risk individuals is *** diagnosis is based on evidence of hepatic steatosis indicated by liver biopsy,imaging,or blood biomarkers,and one of the following conditions:Overweight/obesity,type 2 diabetes mellitus,or metabolic ***,in large-scale epidemiological studies,liver biopsies are not *** application of techniques such as ultrasonography,computed tomography,magnetic resonance imaging,and magnetic resonance spectroscopy is restricted by their limited sensitivity,low effectiveness,high costs,and need for specialized *** biomarkers offer several advantages,particularly in large-scale epidemiological studies or clinical scenarios where traditional imaging techniques are *** of cumulative effects of excess high-normal blood alanine aminotrans-ferase(ALT)levels of blood ALT levels could facilitate identification of at-risk patients who might not be detected through conventional imaging ***,investigating the utility of blood biomarkers in MAFLD should enhance early detection and monitoring,enabling timely inter-vention and management and improving patient outcomes.

关键词： Nonalcoholic fatty liver disease Metabolic dysfunction-associated fatty liver disease Alanine aminotransferase Screening Risk marker

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Recurrence scoring system predicting early recurrence for patients with pancreatic ductal adenocarcinoma undergoing pancreatectomy and portomesenteric vein resection

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World Journal of Gastrointestinal Surgery 2024年第10期16卷 3185-3201页

作者： Hang he Cai-feng Zou yong-Jian Jiang feng Yang Yang di Ji Li chen Jin De-Liang Fu Department of Pancreatic Surgery Huashan HospitalShanghai Medical CollegeFudan UniversityShanghai 200040China

BACKGROUND Pancreatectomy with concomitant portomesenteric vein resection(PVR)enables patients with portomesenteric vein(PV)involvement to achieve radical resection of pancreatic ductal adenocarcinoma,however,early recurrence(ER)is frequently *** To predict ER and identify patients at high risk of ER for individualized *** Totally 238 patients undergoing pancreatectomy and PVR were retrospectively enrolled and were allocated to the training or validating *** Cox and LASSO regression analyses were performed to construct serum recurrence score(SRS)based on 26 serum-derived ***-and multivariate Cox regression analyses of SRS and 18 clinicopathological variables were performed to establish a *** operating characteristic curve analysis was used to evaluate the predictive *** analysis was performed using Kaplan-Meier method and log-rank *** Independent serum-derived recurrence-relevant factors of LASSO regression model,including postoperative carbohydrate antigen 19-9,postoperative carcinoembryonic antigen,postoperative carbohydrate antigen 125,preoperative albumin(ALB),preoperative platelet to ALB ratio,and postoperative platelets to lymphocytes ratio,were used to construct SRS[area under the curve(AUC):0.855,95%CI:0.786–0.924].Independent risk factors of recurrence,including SRS[hazard ratio(HR):1.688,95%CI:1.075-2.652],pain(HR:1.653,95%CI:1.052-2.598),perineural invasion(HR:2.070,95%CI:0.827-5.182),and PV invasion(HR:1.603,95%CI:1.063-2.417),were used to establish the recurrence nomogram(AUC:0.869,95%CI:0.803-0.934).Patients with either SRS>0.53 or recurrence nomogram score>4.23 were considered at high risk for ER,and had poor long-term *** The recurrence scoring system unique for pancreatectomy and PVR,will help clinicians in predicting recurrence efficiently and identifying patients at high risk of ER for individualized therapy.

关键词： Early recurrence Portomesenteric vein resection Pancreatic ductal adenocarcinoma Recurrence score Nomogram

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