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GmAP1d regulates flowering time under long-day photoperiods in soybean

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The Crop Journal 2024年第3期12卷 845-855页

作者： Shiyu Guo Yanfei Li Hongmei Qiu Guoyu Hu Chaosen zhao Ruizhen Wang Hao zhang Yu Tian Xiaoyu Li Bin Liu ying-hui Li Li-juan Qiu college of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina state key laboratory of Crop Gene Resources and Breeding/The National key Facility for Crop Gene Resources and genetic Improvement(NFCRI)/key laboratory of Grain Crop genetic Resources Evaluation and Utilization(MARA) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Jilin Academy of Agricultural sciences/National engineering Research Center for Soybean Changchun 130033JilinChina Crop Research Institute Anhui Academy of Agricultural SciencesHefei 230031AnhuiChina Crops Research Institute of Jiangxi Academy of Agricultural sciences/Jiangxi Province key laboratory of Oilcrops Biology Nanchang 330200JiangxiChina key Lab of Chinese Medicine Resources Conservation State Administration of Traditional Chinese Medicine of the People’s Republic of ChinaInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China

Flowering time is important for adaptation of soybean(Glycine max)to different ***,we conducted a genome-wide association study of flowering time using a panel of 1490 cultivated soybean *** identified three strong signals at the qFT02-2 locus(Chr02:1203.3.9–1223.569),which were associated with flowering time in three environments:Gongzhuling,Mengcheng,and *** analyzing linkage disequilibrium,gene expression patterns,gene annotation,and the diversity of variants,we identified an AP1 homolog as the candidate gene for the qFT02-2 locus,which we named *** one nonsynonymous polymorphism existed among 1490 soybean accessions at position Chr02:*** carrying the Chr02:12087053.T allele flowered significantly earlier than those carrying the Chr02:12087053.A ***,we developed a cleaved amplified polymorphic sequence(CAPS)marker for the SNP at Chr02:12087053.which is suitable for marker-assisted breeding of flowering *** of GmAP1d in the‘Williams 82’background by gene editing promoted flowering under long-day conditions,confirming that GmAP1d is the causal gene for *** analysis of the region surrounding GmAP1d revealed that GmAP1d was artificially selected during the genetic improvement of *** stepwise selection,the proportion of modern cultivars carrying the Chr02:12087053.T allele has increased,and this allele has become nearly fixed(95%)in northern *** findings provide a theoretical basis for better understanding the molecular regulatory mechanism of flowering time in soybean and a target gene that can be used for breeding modern soybean cultivars adapted to different latitudes.

关键词： Soybean Flowering time GWAS GmAP1d Long-day conditions

Characterization and mapping of QTLs on chromosome 2D for grain size and yield traits using a mutant line induced by EMS in wheat

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The Crop Journal 2015年第2期3卷 135-144页

作者： Guizhi zhang yingying Wang ying Guo Yan zhao Fanmei Kong Sishen Li state key laboratory of Crop Biology/Shandong key laboratory of Crop Biology Shandong Agricultural University

Production of mutants with altered phenotypes is a powerful approach for determining the biological functions of genes in an organism. In this study, a high-grain-weight mutant line M8008 was identified from a library of mutants of the common wheat cultivar YN15 treated with ethylmethane sulfonate(EMS). F2 and F2:3.enerations produced from crosses of M8008 × YN15(MY) and M8008 × SJZ54(MS) were used for genetic analysis. There were significant differences between M8008 and YN15 in plant height(PH), spike length(SL),fertile spikelet number per spike(FSS), grain width(GW), grain length(GL), GL/GW ratio(GLW), and thousand-grain weight(TGW). Most simple correlation coefficients were significant for the investigated traits, suggesting that the correlative mutations occurred in M8008. Approximately 21% of simple sequence repeat(SSR) markers showed polymorphisms between M8008 and YN15, indicating that EMS can induce a large number of mutated loci. Twelve quantitative trait loci(QTLs) forming QTL clusters(one in MY and two in MS) were detected. The QTL clusters coinciding with(MY population) or near(MS population) the marker wmc41 were associated mainly with grain-size traits, among which the M8008 locus led to decreases in GW, factor form density(FFD), and TGW and to increases in GLW. The cluster in the wmc25–barc168 interval in the MS population was associated with yield traits, for which the M8008 locus led to decreased PH, spike number per plant(SN), and SL.

关键词： Common wheat Mutant Simple sequence repeat(SSR) Quantitative trait locus(QTL) Grain size trait Yield trait

plant-derived phosphocholine facilitates cellular uptake of anti-pulmonary fibrotic HJT-sRNA-m7

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science China(life sciences) 2019年第3期62卷 309-320页

作者： Jianchao Du Zhu Liang Jiantao Xu Yan zhao Xiaoyun Li Yanli zhang DANDan zhao Ruxuan Chen Yang Liu Trupti Joshi Jiahui Chang Zhiqing Wang Yanxu zhang jindong Zhu Qiang Liu Dong Xu Chengyu Jiang state key laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical SciencesDepartment of Biochemistry Peking Union Medical College Department of Internal Medicine Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Department of Computer science Informatics Institute and Christopher S.Bond Life Science Center University of Missouri Department of Health Management and Informatics and Office of Medical Research School of Medicine University of Missouri state key laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy West China Hospital Sichuan University

Pulmonary fibrosis, a progressive chronic disease with a high mortality rate, has limited treatment options. Currently, lung transplantation remains the only effective treatment. Here we report that a small RNA, HJT-sRNA-m7, from a Chinese herbal medicine Hong Jing Tian(HJT, RHODIOHAE CRENULATAE RADIX ET RHIZOMA, Rhodiola crenulata) can effectively reduce the expressions of fibrotic hallmark genes and proteins both in alveolar in vitro and in mouse lung tissues in vivo. We also discovered over one hundred oil-soluble chemicals from HJT decoctions, most of which are found in lipid extracts from other Chinese herbals decoctions, including Pu Gong ying(PGY, TARAXACI HERBA, Taraxacum mongolicum), Chuan Xin Lian(CXL, changed to "ANDROGRAPHIS HERBA, Andrographis paniculata"), and Jin Yin Hua(JYH, lonicera japonica or Honeysuckle). We identified the active component in these decoctions as two forms of phosphocholines, PC(18:0/18:2) and PC(16:0/18:2). These PCs potentially could form liposomes with small RNAs to enter human alveolar and gastric cells. Our experimental results suggest an unprecendent lipid complex route through which botanic small RNA can enter human *** results provide an innovative treatment strategy for oral delivery of siRNAs as therapeutic medication.

关键词： PC cellular uptake anti-fibrotic HJT sRNA

TaNF-YB11,a gene of NF-Y transcription factor family in Triticum aestivum,confers drought tolerance on plants via modulating osmolyte accumulation and reactive oxygen species homeostasis

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Journal of Integrative Agriculture 2022年第11期21卷 3114-3130页

作者： zhao ying-jia zhang Yan-yang BAI Xin-yang LIN Rui-ze SHI Gui-qing DU Ping-ping XIAO Kai state key laboratory of North China Crop Improvement and Regulation Hebei Agricultural University/College of AgronomyHebei Agricultural UniversityBaoding 071001P.R.China

Transcription factors(TFs)regulate diverse stress defensive-associated physiological processes and plant stress *** characterized TaNF-YB11,a gene of the NF-YB TF family in Triticum aestivum,in mediating plant drought ***-YB11 harbors the conserved domains specified by its NF-YB partners and targets the nucleus after the endoplasmic reticulum(ER)*** two-hybrid assay indicated the interactions of TaNF-YB11 with TaNF-YA2 and TaNF-YC3.two proteins encoded by genes in the NF-YA and NF-YC families,*** results suggested that the heterotrimer established among them further regulated downstream genes at the transcriptional *** transcripts of TaNF-YB11 were promoted in roots and leaves under a 27-h drought ***,its upregulated expression levels under drought were gradually restored following a recovery treatment,suggesting its involvement in plant drought ***-YB11 conferred improved drought tolerance on plants;the lines overexpressing target gene displayed improved phenotype and biomass compared with wild type(WT)under drought treatments due to enhancement of stomata closing,osmolyte accumulation,and cellular reactive oxygen species(ROS)*** expression of TaP5CS2,a P5CS family gene modulating proline biosynthesis that showed upregulated expression in drought-challenged TaNF-YB11 lines,alleviated proline accumulation of plants treated by ***,TaSOD2 and TaCAT3.two genes encoding superoxide dismutase(SOD)and catalase(CAT)that were upregulated underlying TaNF-YB11 regulation,played critical roles in ROS homeostasis via regulating SOD and CAT ***-seq analysis revealed that numerous genes associated with processes of‘cellular processes',‘environmental information processing',‘genetic information processing',‘metabolism',and‘organismal systems'modified transcription under drought underlying control of *** results suggested that the TaNF-YB11-mediated droug

关键词： wheat(Triticum aestivum L.) NF-YB transcription factor drought stress osmolyte accumulation reactive oxygen species(ROS)scavenging

AtTMEM18 plays important roles in pollen tube and vegetative growth in Arabidopsis

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Journal of Integrative plant Biology 2016年第7期58卷 679-692页

作者： Xiao-ying Dou Ke-Zhen Yang zhao-Xia Ma Li-Qun Chen Xue-Qin zhang Jin-Rong Bai De Ye beijing Radiation Center 12 Haidian NanluBeijing 100875China state key laboratory of {3. Physiology and Biochemistry College of Biological SciencesChina Agricultural University2 Yuanmingyuan XiluBeijing 100193China.

In flowering plants, pollen tube growth is essential for delivery of male gametes into the female gametophyte or embryo sac for double fertilization. Although many genes have been identified as being involved in the process, the molecular mechanisms of pollen tube growth remains poorly understood. in this study, we identified that the Arabidopsis Transmembrane protein 18 （AtTMEM18） gene played important roles in pollen tube growth. The AtTMEM18 shares a high similarity with the Transmembrane 18 proteins （TMEM18s） that are conserved in most eukaryotes and may play important roles in obesity in humans. Mutation in the AtTMEM18 by a Ds insertion caused abnormal callose deposition in the pollen grains and had a significant impact on pollen germination and pollen tube growth. AtTMEM18 is expressed in pollen grains, pollen tubes, root tips and other vegetative tissues. The pollen-rescued assays showed that the mutation in AtTMEM18 also caused defects in roots, stems, leaves and transmitting tracts. AtTMEM18-GFP was located around the nuclei. {3. assays demonstrated that the localization of AtTMEM18 around the nuclei in the generative cells of pollen grains was essential for the male ***, expression of the rice TM EM18-homologous protein （OsTMEM18） driven by LAT52 promoter could recover the fertility of the Arabidopsis attmem18 mutant. These results suggested that the TMEM18 is important for plant growth in Arabidopsis.

关键词： Arabidopsis male gametophyte plant pollen TMEM18 transmembrane protein

Conditional QTL Mapping of Sedimentation Volume on Seven Quality Traits in Common Wheat

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Journal of Integrative Agriculture 2013年第12期12卷 2125-2133页

作者： DENG Zhi-ying zhao Liang LIU Bin zhang Kun-pu CHEN Jian-sheng QU Hou-lan SUN Cai-ling zhang Yong-xiang TIAN Ji-chun state key laboratory of Crop Biology Ministry of Science and Technology/Key Laboratory of Crop Biology of Shandong ProvinceDepartment of Agronomy/Group of Wheat Quality BreedingShandong Agricultural University Agricultural Technology Popularization Station state key laboratory of {3. Cell and Chromosome {3. Ministry of Science and Technology/Institute of Genetics and Developmental BiologyChinese Academy of Sciences Central Agricultural Technology Station

To evaluate the possible genetic interrelationships between flour components and the sedimentation volume（SD）,a doubled haploid（DH） population comprising 168 lines were used to identify the conditional quantitative trait loci（QTLs） for SD in three *** additive QTLs and 15 pairs of epistatic QTLs were detected for SD through unconditional and conditional QTL *** major additive QTLs were detected for SD conditioned on the seven quality *** additive QTLs were found to be independent of these *** additive QTLs were suppressed by three of the seven traits because of non-detection in unconditional *** pairs of epistatic QTLs were completely affected by the seven traits because of detection in unconditional mapping but no-detection in conditional *** pairs of epistatic QTLs were detected in conditional *** results indicated that conditional mapping could contribute to a better understanding of the interdependence of different and closely correlated traits at the QTL molecular level,especially some minor QTLs were *** conditional mapping approach provides new insights that will make it possible to avoid the disadvantages of different traits by breeding through molecular design.

关键词： wheat(Triticum aestivum L.) sedimentation volume conditional QTL mapping

Large-scale analysis of small RNAs derived from traditional Chinese herbs in human tissues

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science China(life sciences) 2019年第3期62卷 321-332页

作者： Fengming Huang Jianchao Du Zhu Liang Zhichao Xu Jiantao Xu Yan zhao Yexuan Lin Song Mei Quan He jindong Zhu Qiang Liu Yanxu zhang Yuhao Qin Wei Sun Jingyuan Song Shilin Chen Chengyu Jiang state key laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Department of Biochemistry School of Basic Medicine Peking Union Medical College Tsinghua University Institute of Medicinal plant Development Chinese Academy of Medical Sciences & Peking Union Medical College Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences

plant-derived microRNAs have recently been reported to function in human blood and tissues. Controversy was immediately raised due to possible contamination and the lack of large sample sizes. Here, we report thousands of unique small RNAs derived from traditional Chinese medicine(TCM) herbs found in human blood cells and mouse lung tissues using a large-scale *** extracted small RNAs from decoctions of 10 TCM plants(Ban Zhi Lian, Chai Hu, Chuan Xin Lian, Di Ding Zi Jin, Huang Qin, Jin Yin Hua, Lian Qiao, Pu Gong ying, Xia Ku Cao, and Yu Xing Cao) and obtained millions of RNA sequences from each herb. We also obtained RNA-Seq data from the blood cells of humans who consumed herbal decoctions and from the lung tissues of mice administered RNAs from herbal decoctions via oral gavage. We identified thousands of unique small RNA sequences in human blood cells and mouse lung tissues. Some of these identified small RNAs from Chuan Xin Lian and Hong Jing Tian could be mapped to the genomes of the herbs, confirming their TCM plant origin. Small RNAs derived from herbs regulate mammalian gene expression in a sequence-specific manner, and thus are a superior novel class of herbal drug components that hold great potential as oral gene-targeted therapeutics, highlighting the important role of herbgenomics in their development.

关键词： small RNA herbal decoction cross-kingdom regulation miRNA traditional Chinese medicine gene-targeted therapy

Genome-wide analysis of OVATE family proteins in cucumber(Cucumis sativus L.)

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Journal of Integrative Agriculture 2022年第5期21卷 1321-1331页

作者： HAN Li-jie SONG Xiao-fei WANG Zhong-yi LIU Xiao-feng YAN Li-ying HAN De-guo ZHOU zhao-yang zhang Xiao-lan state key Laboratories of Agrobiotechnology/beijing key laboratory of Growth and Developmental Regulation for Protected Vegetable Crops/MOE Joint laboratory for International Cooperation in Crop Molecular Breeding/China Agricultural university Beijing 100193P.R.China Analysis and Testing Centre Hebei Normal University of Science&TechnologyQinhuangdao 066004P.R.China college of Horticulture science and Technology Hebei Normal University of Science&TechnologyQinhuangdao 066004P.R.China key laboratory of Biology and genetic Improvement of Horticultural Crops of Northeast Region Ministry of Agriculture and Rural Affairs/College of Horticulture&Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030P.R.China

OVATE family proteins(OFPs)are plant-specific proteins with a conserved OVATE domain that regulate plant growth and *** OFPs have been studied in several species,their biological functions remain largely unknown in cucumber(Cucumis sativus L.).This study identified 19 Cs OFPs distributed on seven chromosomes in *** Cs OFP genes were expressed in reproductive organs,but with different expression *** expression of Cs OFP12-16c in Arabidopsis resulted in shorter and blunt *** overall results indicated that Cs OFP12-16c regulates silique development in Arabidopsis and may have a similar function in cucumber.

关键词： cucumber OVATE family proteins expression pattern Cs OFP12-16c fruit development

PuHox52 promotes coordinated uptake of nitrate,phosphate, and iron under nitrogen deficiency in Populus ussuriensis

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Journal of Integrative plant Biology 2023年第3期65卷 791-809页

作者： Ming Wei Mengqiu zhang Jiali Sun ying zhao Solme Pak Miaomiao Ma yingxi Chen Han Lu Jingli Yang Hairong Wei Yuhua Li Chenghao Li state key laboratory of Tree {3.s and Breeding School of ForestryNortheast Forestry UniversityHarbin 150040China college of life sciences Northeast Forestry UniversityHarbin 150040China college of Forest Resources and Environmental science Michigan Technological UniversityHoughtonMichigan 49931USA key laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry university) Ministry of EducationHarbin 150040China

It is of great importance to better understand how trees regulate nitrogen(N) uptake under N deficiency conditions which severely challenge afforestation practices, yet the underlying molecular mechanisms have not been well elucidated. Here,we functionally characterized PuHox52, a Populus ussuriensis HD-ZIP transcription factor, whose overexpression greatly enhanced nutrient uptake and plant growth under N deficiency. We first conducted an RNA sequencing experiment to obtain root transcriptome using PuHox52-overexpression lines of P. ussuriensis under low N treatment. We then performed multiple genetic and phenotypic analyses to identify {3. target genes of PuHox52 and validated how they acted against N deficiency under PuHox52 ***52 was specifically induced in roots by N deficiency, and overexpression of PuHox52promoted N uptake, plant growth, and root development. We demonstrated that several nitrate-responsive genes(PuNRT1.1, PuNRT2.4,PuCLC-b, PuNIA2, PuNIR1, and PuNLP1),phosphate-responsive genes(PuPHL1A and PuPHL1B), and an iron transporter gene(PuIRT1) were substantiated to be direct targets of PuHox52. Among them, PuNRT1.1, PuPHL1A/B, and PuIRT1 were upregulated to relatively higher levels during PuHox52-mediated responses against N deficiency in PuHox52-overexpression lines compared to WT. Our study revealed a novel regulatory mechanism underlying root adaption to N deficiency where PuHox52 modulated a coordinated uptake of nitrate, phosphate, and iron through 'PuHox52-PuNRT1.1', 'PuHox52-PuPHL1A/PuPHL1B', and'PuHox52-PuIRT1' regulatory relationships in poplar roots.

关键词： HD-Zip transcription factor iron transport nitrate transport nitrogen deprivation phosphate absorption Populus ussuriensis root architecture target gene