检索结果-南通市图书馆

Protein Containing the GGDEF Domain Affects Motility and Biofilm Formation in Vibrio cholerae and is Negatively Regulated by Fur and HapR

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2023年第10期36卷 949-958页

作者： GAO He MA Li Zhi QIN Qin CUI Yao MA Xiao Han zhang yi quan KAN Biao State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Medical Risk Management Section Third Medical CentreChinese PLA(People’s Liberation Army)General HospitalBeijing 100039China Department of Clinical Laboratory Affiliated Nantong Hospital 3 of Nantong UniversityNantong Third People's HospitalNantong 226006JiangsuChina 3.Department of Clinical LaboratoryAffiliated Nantong Hospital 3 of Nantong UniversityNantong Third People's Hospital

Objective This study aimed to investigate whether the VCA0560 gene acts as an active diguanylate cyclase(DGC)in Vibrio cholerae and how its transcription is regulated by Fur and Hap *** The roles of VCA0560 was investigated by utilizing various phenotypic assays,including colony morphological characterization,crystal violet staining,Cyclic di-GMP(c-di-GMP)quantification,and swimming motility *** regulation of the VCA0560 gene by Fur and Hap R was analyzed by luminescence assay,electrophoretic mobility shift assay,and DNase I *** VCA0560 gene mutation did not affect biofilm formation,motility,and c-di-GMP synthesis in ***,and its overexpression remarkably enhanced biofilm formation and intracellular c-di-GMP level but reduced motility *** transcription of the VCA0560 gene was directly repressed by Fur and the master quorum sensing regulator Hap *** Overexpressed VCA0560 functions as an active DGC in ***,and its transcription is repressed by Fur and Hap R.

关键词： Vibrio cholera Cyclic di-GMP VCA0560 Fur HapR

Transcriptional Regulation of YP3680 by Rov A and Rcs AB in Yersinia pestis biovar Microtus

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2022年第3期35卷 234-238页

作者： LIU Lei FANG Nan LIU Wan Bing HE ying Yu LIU Yan zhang yi quan Department of Transfusion Medicine General Hospital of Central Theater Command of the PLAWuhan 430070HubeiChina The First School of Clinical Medicine Southern Medical UniversityGuangzhou 510280GuangdongChina Beijing USCI Medical Laboratory Co. Ltd.Building C7YiYuan Industrial BaseBeijing 100195China Department of Clinical Laboratory Nantong Third Hospital Affiliated to Nantong UniversityNantong 226006JiangsuChina

Type-VI secretion system(T6SS)is a widespread bacteriophage-like complex in bacteria that participates in multiple physiological processes,including metal ion uptake,bacterial competition,and biofilm formation^([1]).Y... 详细信息

关键词： agent Regulation competition

Characterization of the CpsQ Regulon Reveals Its Role in the Transcription of Type Ⅵ Secretion System 2 Genes in Vibrio parahaemolyticus

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2024年第4期37卷 436-439页

作者： LUO Xi LI Xue zhang Miao Miao HUANG Qing Lian zhang yi quan JI Shen Jie LU Ren Fei Department of Clinical Laboratory Affiliated Nantong Hospital 3 of Nantong UniversityNantong Third People’s HospitalNantong 226006JiangsuChina Department of Clinical Laboratory Qidong People’s HospitalQidong 226200JiangsuChina

Vibrio parahaemolyticus,a gram-negative halophilic bacterium that naturally inhabits coastal waters,causes gastroenteritis,skin infections,and septicemia in human beings[1].This bacterium produces multiple virulence factors,including thermostable direct hemolysin(TDH),TDH-related hemolysin(TRH),type Ⅲ secretion system 1(T3SS1),T3SS2,type Ⅵ secretion system 1(T6SS1),and T6SS2[1].Furthermore,Ⅴ.parahaemolyticus forms biofilms on the surface,which help it in adapting to unfavorable conditions[2].Mature biofilm formation requires special structures,including lateral and polar flagella,exopolysaccharide(EPS),and type Ⅳ pili[2].

关键词： coastal system T3SS

Optimized Methods for Biofilm Analysis in Yersinia pestis

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2013年第5期26卷 408-411页

作者： FANG Nan GAO He WANG Li QU Shi zhang yi quan YANG Rui Fu ZHOU Dong Sheng State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Centre for Disease Control and Prevention

Transmission of Yesinia pestis relies primarily on the bite of flea vectors,which is closely related to the biofilm formation of this pathogen[1].*** synthesizes heavily the attached biofilms,a population of bacterial colonies embedded in self-produced exopolysaccharide matrix,in the flea proventriculus[1].The biofilm formation leads to the blockage of fleas,making the fleas feel hungry and repeatedly attempt to feed,and thus the plague

关键词： Figure

Master Quorum Sensing Regulator HapR Acts as A Repressor of the Mannitol Phosphotransferase System Operon in Vibrio cholerae

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2022年第1期35卷 69-72页

作者： zhang yi quan MA Li Zhi GAO Yue QIN Qin LI Jie LOU Jing zhang Miao Miao XUE Xing Fan KAN Biao GAO He Department of Clinical Laboratory Nantong Third Hospital Affiliated to Nantong UniversityNantong 212006JiangsuChina Medical Risk Management Section Third Medical CentreChinese PLA(People’s Liberation Army)General HospitalBeijing 100039China First Affiliated Hospital of Dalian Medical University Dalian 116011LiaoningChina State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China School of Medicine Jiangsu UniversityZhenjiang 212013JiangsuChina

The phosphoenolpyruvate(PEP):carbohydrate phosphotransferase system(PTS)is widely used by bacteria to take up sugars or sugar derivatives;.PTS is usually composed of a histidine protein(HPr),enzyme I(EI),and several e... 详细信息

关键词： sugar system usual

Use of Rich BHI Medium Instead of Synthetic TMH Medium for Gene Regulation Study in Yersinia pestis

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2012年第6期25卷 639-644页

作者： zhang yi quan MA Li Zhi WANG Li GAO He TAN Ya Fang GUO Zhao Biao QIU Jing Fu YANG Rui Fu ZHOU Dong Sheng State Key Laboratory of Pathogen and Biosecurity Beo'ing Institute of Microbiology and Epidemiology Beijing 100071 China Department of Emergency Medicine General Hospital of Chinese People's Armed Police Forces Beijing 100039 China Department of Medical Laboratory Chongqing Medical University Chongqing 400016 China State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention Chinese Centre for Disease Control and Prevention Beijing 102206 China

Objective This study is to verify the use of rich BHI medium to substitute synthetic media for gene regulation studies in Yersinia pestis. Methods The transcriptional regulation of rovA by PhoP or via temperature upshift, and that of pla by CRP were investigated when Y. pestis was cultured in BHI. After cultivation under 26 ~C, and with temperature shifting from 26 to 37 ~C, the wild-type （WT） strain or its phoP or crp null mutant （AphoP or Acrp, respectively） was subject to RNA isolation, and then the promoter activity of rovA or plo in the above strains was detected by the primer extension assay. The rovA promoter-proximal region was cloned into the pRW50 containing a promoterless lacZ gene. The recombinant LacZ reporter plasmid was transformed into WT and AphoP to measure the promoter activity of rovA in these two strains with the ^-Galactosidase enzyme assay system. Results When Y. pestis was cultured in BHI, the transcription of rovA was inhibited by PhoP and upon temperature upshift while that ofpla was stimulated by CRP. Conclusion The rich BHI medium without the need for modification to be introduced into the relevant stimulating conditions （which are essential to triggering relevant gene regulatory cascades）, can be used in lieu of synthetic TMH media to cultivate Y. pestis for gene regulation studies.

关键词： Yersinia pestis BHI TMH Gene regulation

ToxR Is Required for Biofilm Formation and Motility of Vibrio Parahaemolyticus

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2018年第11期31卷 848-850页

作者： CHEN Long QIU Yue TANG Hao HU Ling Fei YANG Wen Hui ZHU Xiao Jue HUANG Xin Xiang WANG Tang zhang yi quan Department of Clinical Laboratory the First People's Hospital of Zhangjiagang School of Medicine Jiangsu University State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology School of Health Sciences Wuhan University

Vibrio parahaemolyticus, the leading cause of seafood-borne gastroenteritis, has the ability to form biofilms on biotic and abiotic surfaces. Biofilm formation is a complicated process involving many specific structures and regulatory processes. The most significant of the structures and processes include polar and lateral flagella, mannose-sensitive hemagglutinin typeⅣpili, chitin-regulated pili,capsular polysaccharide (CPS), exopolysaccharide

关键词： WT Figure CPS ToxR Is Required for Biofilm Formation and Motility of Vibrio Parahaemolyticus

H-NS Represses Biofilm Formation and c-di-GMP Synthesis in Vibrio parahaemolyticus

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2022年第9期35卷 821-829页

作者： XUE Xing Fan ZHNAG Miao Miao SUN Jun Fang LI Xue WU Qi Min yiN Zhe YANG Wen Hui NI Bin HU Ling Fei ZHOU Dong Sheng LU Ren Fei zhang yi quan School of Medicine Jiangsu UniversityZhenjiang 212013JiangsuChina Department of Clinical Laboratory Affiliated Nantong Hospital 3 of Nantong UniversityNantong 226006JiangsuChina State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China

Objective This study aimed to investigate the regulation of histone-like nucleoid structuring protein(H-NS)on biofilm formation and cyclic diguanylate(c-di-GMP)synthesis in Vibrio parahaemolyticus *** Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining,quantification of c-di-GMP,quantitative real-time polymerase chain reaction,LacZ fusion,and electrophoretic-mobility shift *** The deletion of hns enhanced the biofilm formation and intracellular c-di-GMP levels in *** RIMD2210633.H-NS can bind the upstream promoter-proximal DNA regions of scrA,scrG,VP0117,VPA0198,VPA1176,VP0699,and VP2979 to repress their *** genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP *** One of the mechanisms by which H-NS represses the biofilm formation by *** RIMD2210633 may be via repression of the production of intracellular c-di-GMP.

关键词： Vibrio parahaemolyticus Biofilm H-NS C-di-GMP

Prediction and optimization of flue pressure in sintering process based on SHAP

在线全文

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 346-359页

作者： Mingyu Wang Jue Tang Mansheng Chu quan Shi Zhen zhang School of Metallurgy Northeastern UniversityShenyang 110819China Liaoning Low-Carbon Steelmaking Technology Engineering Research Center Northeastern UniversityShenyang 110819China Engineering Research Center of Frontier Technologies for Low-Carbon Steelmaking(Ministry of Education) Shenyang 110819China

Sinter is the core raw material for blast *** pressure,which is an important state parameter,affects sinter *** this paper,flue pressure prediction and optimization were studied based on the shapley additive explanation(SHAP)to predict the flue pressure and take targeted adjustment ***,the sintering process data were collected and processed.A flue pressure prediction model was then constructed after comparing different feature selection methods and model algorithms using SHAP+extremely random-ized trees(ET).The prediction accuracy of the model within the error range of±0.25 kPa was 92.63%.SHAP analysis was employed to improve the interpretability of the prediction *** effects of various sintering operation parameters on flue pressure,the relation-ship between the numerical range of key operation parameters and flue pressure,the effect of operation parameter combinations on flue pressure,and the prediction process of the flue pressure prediction model on a single sample were analyzed.A flue pressure optimization module was also constructed and analyzed when the prediction satisfied the judgment *** operating parameter combination was then *** flue pressure was increased by 5.87%during the verification process,achieving a good optimization effect.

关键词： sintering process flue pressure shapley additive explanation prediction optimization