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Nanobodies against African swine fever virus p72 and CD2v proteins as reagents for developing two cELISAs to detect viral antibodies

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Virologica Sinica 2024年第3期39卷 478-489页

作者： Jiahong Zhu Qingyuan Liu Liuya Li Runyu zhang Yueting Chang Jiakai Zhao Siyu Liu Xinyu Zhao Xu Chen yani Sun Qin Zhao Department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F UniversityYangling Observing and Experimental Station of National Data Center of Animal HealthMinistry of AgricultureYangling712100China

African swine fever virus(ASFV)poses a significant threat to the global swine ***,there are no effective vaccines or treatments available to combat ASFV infection in *** primary means of controlling the spread of the disease is through rapid detection and subsequent elimination of infected ***,a lower virulent ASFV isolate with a deleted EP402R gene(CD2v-deleted)has been reported in China,which further complicates the control of ASFV infection in pig ***,an EP402R-deleted ASFV variant has been developed as a potential live attenuated vaccine candidate ***,it is crucial to develop detection methods that can distinguish wild-type and EP402R-deleted ASFV *** this study,two recombinant ASFV-p72 and-CD2v proteins were expressed using a prokaryotic system and used to immunize Bactrian ***,eight nanobodies against ASFV-p72 and ten nanobodies against ASFV-CD2v were *** the production of these nanobodies with horse radish peroxidase(HRP)fusion proteins,the ASFV-p72-Nb2-HRP and ASFV-CD2v-Nb22-HRP fusions were selected for the development of two competitive ELISAs(cELISAs)to detect anti-ASFV *** two cELISAs exhibited high sensitivity,good specificity,repeatability,and *** coincidence rate between the two cELISAs and commercial ELISA kits was 98.6%and 97.6%,***,the two cELISA for detecting antibodies against ASFV demonstrated ease of operation,a low cost,and a simple production *** two cELISAs could determine whether pigs were infected with wild-type or CD2v-deleted ASFV,and could play an important role in monitoring ASFV infections in pig farms.

关键词： African swine fever virus(ASFV) ASFV-p72 ASFV-CD2v Nanobody-HRP Competitive ELISA

Teaching Reform Exploring of Vocational Education Courses with the Guidance of "Military Training and Competition"

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Teaching Reform Exploring of Vocational Education Courses wi...

第四届人文科学、管理和教育技术国际学术会议

作者： yani zhang Lei Qin Xiaoshuang Wang Xuejun Rao College of Information and Communication National University of Defense Technology

Focusing on the instructions "Carry Out the Mass Activities of Military Training and Competition" of President Xi [1],oriented by war preparation and driven by the contemporary spirit of "Military Training and Competition",this paper explores a comprehensive multi-level teaching reform and innovation of vocational education practice courses including teaching contents,training methods,resources and evaluation mechanism.

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无人侦察机航迹点重规划的优化算法

北京航空航天大学学报 2010年第1期36卷 35-38页

作者：张雅妮高金源北京航空航天大学自动化科学与电气工程学院北京100191

提出了一种针对发现新目标后,机上在线进行航迹点重规划的方法.建立了重规划数学模型,用图像质量方程预估侦察目标的图像质量,以所有目标图像质量的加权之和最大、路径长度最小为目标函数.在传感器侦察范围、飞机机动和目标图像质量要... 详细信息

提出了一种针对发现新目标后,机上在线进行航迹点重规划的方法.建立了重规划数学模型,用图像质量方程预估侦察目标的图像质量,以所有目标图像质量的加权之和最大、路径长度最小为目标函数.在传感器侦察范围、飞机机动和目标图像质量要求以及威胁等多约束条件下运用改进后的多目标进化算法NSGA-II寻优,得到侦察航迹点的Pareto最优解集.以目标图像质量、路径长度和路径威胁为评价因素,专家系统给出当前评价因素加权权值,用模糊选优方法在Pareto最优解集中选择该加权意义下的唯一最优路径.仿真结果证明了该方法的可行性和智能性.

关键词：图像质量方程多目标优化专家系统模糊选优

“Internet+” Military Vocational Education: Logical connotation and Implementation path

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Mechanism of Guangdong Shenqu in regulating intestinal flora in mice with food stagnation and internal heat based on 16S rDNA sequencing Author links open overlay panel

第四届人文科学与社会发展国际学术会议

作者： Zhao Jing XiaoShuang Wang XinShe Qi yani zhang School of Information and Communication National University of Defense Technology

"Internet+", is diffusing into and changing the field of education. It also provides realistic conditions and unprecedented opportunities for the reform and innovation of military vocational education. This paper discusses the implementation logic and development path of "Internet + " military vocational education from the perspectives of reshaping educational concepts, perfecting educational laws and regulations, innovating schooling models. The purposes of this paper are to inform the theoretical research and the practices in military vocational education, and to promote the development of military vocational education.

关键词： "Internet+" Education Military Vocational Education Logical connotation

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Journal of Traditional Chinese Medical Sciences 2024年第2期11卷 232-244页

作者： yani Jiang Zhenhong zhang Han Chen Yanmin Wang Yanrui Xu Zijie Chen Yan Xu Jingjuan Wang School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing102488China School of Chinese Traditional Medicine Beijing University of Chinese MedicineBeijing102488China Department of Chemistry Cleveland State UniversityCleveland44115USA

Objective To investigate the effect of Guangdong Shenqu(GSQ)on intestinal flora structure in mice with food stagnation through 16S rDNA *** Mice were randomly assigned to control,model,GSQ low-dose(GSQL),GSQ medium-dose(GSQM),GSQ high-dose(GSQH),and lacidophilin tablets(LAB)groups,with each group containing 10 mice.A food stagnation and internal heat mouse model was established through intragastric administration of a mixture of beeswax and olive oil(1:15).The control group was administered normal saline,and the model group was administered beeswax and olive oil to maintain a *** GSQL(2 g/kg),GSQM(4 g/kg),GSQH(8 g/kg),and LAB groups(0.625 g/kg)were administered corresponding drugs for 5 *** administration,16S rDNA sequencing was performed to assess gut microbiota in mouse fecal *** The model group exhibited significant intestinal flora *** GSQ administration,the abundance and diversity index of the intestinal flora increased significantly,the number of bacterial species was regulated,andαandβdiversity were *** administration increased the abundance of probiotics,including Clostridia,Lachnospirales,and Lactobacillus,whereas the abundance of conditional pathogenic bacteria,such as Allobaculum,Erysipelotrichaceae,and Bacteroides *** prediction analysis indicated that the pathogenesis of food stagnation and GSQ intervention were primarily associated with carbohydrate,lipid,and amino acid metabolism,among other metabolic *** The digestive mechanism of GSQ may be attributed to its role in restoring diversity and abundance within the intestinal flora,thereby improving the composition and structure of the intestinal flora in mice and subsequently influencing the regulation of metabolic pathways.

关键词： Guangdong Shenqu Food stagnation Gut microbiota High-throughput gene sequencing Species annotation Metabolic pathway

从“平教运动”到“新秧歌运动”:传统秧歌的舞台化

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文化遗产 2020年第6期 123-128页

作者：张娅妮山东大学(威海)艺术学院舞蹈系山东大学文化遗产研究院山东青岛266237

中国传统秧歌的舞台化,是在1920年代以晏阳初、梁漱溟、陶行知等知识精英领导下的“平教运动”中萌芽,并在“新秧歌运动”的接续中得以最终实现。“平教运动”是传统秧歌舞台化的萌芽期,平民教育实验延续了北大歌谣时期所倡导的对民间... 详细信息

中国传统秧歌的舞台化,是在1920年代以晏阳初、梁漱溟、陶行知等知识精英领导下的“平教运动”中萌芽,并在“新秧歌运动”的接续中得以最终实现。“平教运动”是传统秧歌舞台化的萌芽期,平民教育实验延续了北大歌谣时期所倡导的对民间文化“学术的”与“文艺的”研究路线并通过“政教合一”的提出使民间文化开始与政治结合,显现出民间文化与政治的互动。“新秧歌运动”是接续“平教运动”对传统秧歌的进一步认定与征用,在此过程中,传统秧歌不仅摆脱了“旧”样貌获得了“新”身份,还以其强大的草根力量为抗日战争提供了发动民众的有效形式并成功影响了共产党人知识精英对民众的认识,并在艺术化的实践中最终获得了“舞台化”的合法性。传统秧歌在这两场文化运动的影响与知识精英的选择下被启蒙、被征用,在文化运动、知识精英与民间文化的复杂勾连中最终实现了舞台化。

关键词：传统秧歌 “平教运动” “新秧歌运动” 知识精英舞台化

Theoretical study of novel dual-core microstructured photonic crystal fiber

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Theoretical study of novel dual-core microstructured photoni...

2015年（第七届）西部光子学学术会议

作者： Qiang Xu Miao Wang Shebao Lin Zhihuai Yang yani zhang Lei zhang

We have theoretically investigated a novel dual-core microstructured photonic crystal fiber, which consist of two cores and a rectangular lattice cladding. The full-vector the plane wave expansion method is applied to analyze its high birefringence and dispersion. The simulation result shows that negative dispersion slope values in C band and high birefringence（10-2） are obtained. The new structure is compact in size and easy to fabricate, making it promising for miniaturized complex communication devices.

关键词： Dual-core photonic crystal fibers Birefringence Dispersion

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超宽带微型无人机数据链传输性能分析

西北工业大学学报 2009年第2期27卷 245-249页

作者：何一张亚妮王永生西北工业大学电子信息学院陕西西安710072 西安通信学院陕西西安710106

论证了低速率二进制跳时超宽带(TH-UWB)和直序列超宽带(DS-UWB)技术适用于微型无人机数据链系统。针对不同误码率和传输失效概率,建立了TH-UWB和DS-UWB在自由空间、对数正态阴影衰落以及频率选择性双径信道下的传播模型,推导出了传输距... 详细信息

论证了低速率二进制跳时超宽带(TH-UWB)和直序列超宽带(DS-UWB)技术适用于微型无人机数据链系统。针对不同误码率和传输失效概率,建立了TH-UWB和DS-UWB在自由空间、对数正态阴影衰落以及频率选择性双径信道下的传播模型,推导出了传输距离与数据速率的关系。仿真结果表明:在AWGN信道下,二进制DS-UWB相对TH-UWB可以获得更远的传输距离;飞行区域的阴影衰落造成UWB系统接收端信号起伏,信号传输性能与自由空间传播相比有所下降;采用频率选择性双径损耗模型在特定距离范围内会改善系统性能。

关键词：超宽带微型无人机无人机数据链无线电传播

Na^+对尖晶石结构Co_((1-x))Na_xCr_2O_4电子结构及光学性质的影响

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光学学报 2014年第11期34卷 34-43页

作者：杨志怀张云鹏张美光许强张亚妮张蓉西安理工大学材料科学与工程学院陕西西安710048 宝鸡文理学院物理与信息技术系陕西宝鸡721016 西北工业大学理学院陕西西安710072

采用基于密度泛函理论的平面波超软赝势法,系统研究了Na+四面体掺杂Co(1-x)NaxCr2O4体系的基态结构参数、能带结构、电子态密度、磁矩和光学性质。当Na+掺杂离子数分数x为0.125时,计算结果表明Na+四面体掺杂(Co位)比八面体掺杂(Cr位)容... 详细信息

采用基于密度泛函理论的平面波超软赝势法,系统研究了Na+四面体掺杂Co(1-x)NaxCr2O4体系的基态结构参数、能带结构、电子态密度、磁矩和光学性质。当Na+掺杂离子数分数x为0.125时,计算结果表明Na+四面体掺杂(Co位)比八面体掺杂(Cr位)容易进行。对Na+四面体掺杂体系Co(1-x)NaxCr2O4,计算发现,随着Na+掺杂离子数分数增加,体系的晶格参数逐渐增大,晶胞的磁矩和禁带宽度均减小。同时,费米能级进入价带部分更深。此外,光学性质的结果显示Na+可以使CoCr2O4的吸收光谱发生红移,并在低能区有很强的吸收,表明Na+能极大地提高CoCr2O4对可见光的吸收和光催化效率。

关键词：原子与分子物理密度泛函理论第一性原理电子结构光学性质尖晶石结构CoCr2O4