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一种新的鸡胚尿囊膜模型的建立与应用

西安交通大学学报（医学版） 2011年第5期32卷 589-591,609页

作者：张彦民王红英西安交通大学医学院药物研究所陕西西安710061

目的建立一种新的鸡胚尿囊膜模型并考察氢化可的松对鸡胚尿囊膜血管生成的影响。方法运用假气室法建立鸡胚尿囊膜模型,采用硅胶环、甲基纤维素、明胶海绵、玻璃纤维滤纸为给药载体进行考察。结果用硅胶环作为载体建立的鸡胚尿囊膜实验模... 详细信息

目的建立一种新的鸡胚尿囊膜模型并考察氢化可的松对鸡胚尿囊膜血管生成的影响。方法运用假气室法建立鸡胚尿囊膜模型,采用硅胶环、甲基纤维素、明胶海绵、玻璃纤维滤纸为给药载体进行考察。结果用硅胶环作为载体建立的鸡胚尿囊膜实验模型,是一种较理想的研究抗血管生成药物的筛选模型。硅胶环作为载体与明胶海绵和纤维滤纸载体等相比,具有效果直观,加样量准确,在显微镜下易于观察、照相的优点。氢化可的松对鸡胚尿囊膜新生血管生成具有较好的抑制作用,呈现出较好的量效关系。结论本研究建立了一种新的鸡胚尿囊膜模型,运用该模型得出氢化可的松对鸡胚尿囊膜血管生成有明显的抑制作用。

关键词：血管生成鸡胚尿囊膜抑制剂氢化可的松

A mitochondria targeting Ir(Ⅲ)complex triggers ferroptosis and autophagy for cancer therapy:A case of aggregation enhanced PDT strategy for metal complexes

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Chinese Chemical Letters 2025年第1期36卷 374-380页

作者： Panpan Wang Hongbao Fang Mengmeng Wang Guandong zhang Na Xu yan Su Hongke Liu Zhi Su Jiangsu Collaborative Innovation Center of Biomedical Functional Materials College of Chemistry and Materials ScienceNanjing Normal UniversityNanjing 210023China

Metal complexes hold significant promise in tumor diagnosis and ***,their potential applications in photodynamic therapy(PDT)are hindered by issues such as poor photostability,low yield of reactive oxygen species(ROS),and aggregation-induced ROS *** address these challenges,we present a molecular self-assembly strategy utilizing aggregation-induced emission(AIE)conjugates for metal *** a proof of concept,we synthesized a mitochondrial-targeting cyclometalated Ir(Ⅲ)photosensitizer *** approach significantly enhances the photodynamic effect while mitigating the dark toxicity associated with AIE ***-TPE readily self-assembles into nanoaggregates in aqueous solution,leading to a significant production of ROS upon light *** Ir-TPE triggers multiple modes of death by excessively accumulating ROS in the mitochondria,resulting in mitochondrial DNA *** damage can lead to ferroptosis and autophagy,two forms of cell death that are highly cytotoxic to cancer *** aggregation-enhanced photodynamic effect of Ir-TPE significantly enhances the production of ROS,leading to a more pronounced cytotoxic *** vitro and in vivo experiments demonstrate this aggregation-enhanced PDT approach achieves effective in situ tumor *** study not only addresses the limitations of metal complexes in terms of low ROS production due to aggregation but also highlights the potential of this strategy for enhancing ROS production in PDT.

关键词： Metal complex AIEgens Mitochondria targeting Enhanced photodynamic therapy Anticancer agent

人表皮生长因子基因真核表达载体的构建及其基因转染

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中国临床康复 2006年第29期10卷 86-88,i0003页

作者：鲍玉洲张立华张艳敏河南省眼科研究所分子生物室河南省角膜病重点实验室河南省郑州市450003

目的:构建人表皮生长因子基因真核表达载体,并将其导入细胞中表达表皮生长因子重组蛋白。方法:经HindⅢ、BamHI酶切PGEM-TEasy-hEGF,将人表皮生长因子基因定向克隆到真核表达载体pcDNA3.1中。在脂质体的包裹下将pcDNA3.1-hEGF导入角膜... 详细信息

目的:构建人表皮生长因子基因真核表达载体,并将其导入细胞中表达表皮生长因子重组蛋白。方法:经HindⅢ、BamHI酶切PGEM-TEasy-hEGF,将人表皮生长因子基因定向克隆到真核表达载体pcDNA3.1中。在脂质体的包裹下将pcDNA3.1-hEGF导入角膜组织细胞中,分别从DNA、RNA、蛋白质3个水平上检测人表皮生长因子基因表达情况。结果:经T7/SP6和hEGF内套引物进行PCR扩增及酶切鉴定重组体,分别得到310bp和162bp两条基因片段,将162bp条带经纯化后用AVaⅢ限制性内切酶切鉴定,可分别得到89bp和72bp两条片段;将人表皮生长因子基因序列与Genbank上公布的序列相比,碱基同源性达98.2%,氨基酸完全相同;PCR,RT-PCR在转染后21d之内,在DNA、RNA水平上可检测到人表皮生长因子基因;免疫组织化学检测法在转染后第3天可检测到人表皮生长因子蛋白表达,2周之后,人表皮生长因子蛋白表达结果转为阴性。结论:pcDNA3.1-hEGF重组质粒与LipofectamineTM2000以适当比例混合形成的微囊可携带人表皮生长因子基因进入细胞内并表达功能蛋白。

关键词：表皮生长因子基因转染

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俄罗斯农业改革与中俄农业合作

东北亚论坛 2024年第5期33卷 111-126,128页

作者：姚毓春张艳敏李冰吉林大学中国国有经济研究中心暨经济学院长春130012 吉林大学乡村振兴研究院吉林大学经济学院长春130012 西南大学马克思主义学院重庆400715

苏联由于片面强调发展重工业而忽视农业,导致农产品极度短缺。苏联解体后,俄罗斯进行了一系列农业改革,农业资源禀赋优势得以逐步发挥,并成功跻身世界农业大国之列。坚实的农业基础是保障俄罗斯在面对俄乌冲突局势升级、西方国家对其采... 详细信息

苏联由于片面强调发展重工业而忽视农业,导致农产品极度短缺。苏联解体后,俄罗斯进行了一系列农业改革,农业资源禀赋优势得以逐步发挥,并成功跻身世界农业大国之列。坚实的农业基础是保障俄罗斯在面对俄乌冲突局势升级、西方国家对其采取极限经济制裁的情况下,仍能保持经济平稳和社会稳定的重要原因之一。回顾俄罗斯农业改革历程,可以分为以私有化和市场化为导向的激进改革、以减负和扶持为主要手段的政策调整、以安全和高效为目标的深化改革三个阶段。通过对俄罗斯农业改革历程进行回顾和梳理可以判断,在未来较长的一段时期内,俄罗斯农业政策趋于稳定,这可以为中俄两国农业合作提供一个相对稳定和清晰的政策预期。同时,由于俄罗斯农业发展的政策方向与我国农业发展的大方向在一定程度上具有相似性,加之中俄关系持续向好,两国具备开展农业合作的有利条件。未来,中俄两国要加强农业合作顶层设计,建立多层次农业合作体系,完善两国在农业投资方面的法律政策,不断完善中俄两国农业合作模式。

关键词：俄罗斯农业改革粮食安全农业合作俄乌冲突

Potassium ions and cyano group modified g-C_(3)N_(4) for effective generation of H_(2)O_(2) through two-electron oxygen reduction

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Science China Chemistry 2025年第1期68卷 192-200页

作者： Dongying Wang Shulan Pu Yongmin Chen Ke Lei Yujie Duan Linjiao Mao Xuhui Zeng Xi Luo Yuntao zhang Yuqin Dong Jin Zhong zhang yan Sun School of Mechanical Engineering Chengdu UniversityChengdu 610106China Department of Chemistry and Biochemistry University of CaliforniaSanta CruzCA 95064USA

Potassium ions(K^(+))doped graphitic carbon nitride(g-C_(3)N_(4))was prepared by a thermal etching method using potassium hydroxide(KOH)as an ion *** transform infrared spectroscopy(FTIR)and X-ray photoelectron spectroscopy(XPS)results showed that the generation of the cyano group was detected while introducing K^(+).Under simulated sunlight irradiation,the sample with a K^(+)doping amount of 10%showed the highest hydrogen peroxide(H_(2)O_(2))generation rate of2,140.2μmol h^(-1)g^(-1).The apparent quantum yield(AQY)at 400 nm and the solar-to-chemical conversion(SCC)are 4.35%and1.23%,respectively.K^(+)acted as a bridge between g-C_(3)N_(4)layers,which enhanced charge transfer ***,the cyano group enhanced the adsorption capacity of protons(H^(+))and promoted the yield of H_(2)O_(2).The catalyst exhibited excellent photocatalytic stability based on four-cycle *** addition,a mechanism study showed that superoxide radicals(·O_(2)^(-))were the most important active species in the reaction *** production of H_(2)O_(2)was achieved through consecutive single-electron *** study deepens the understanding of the oxygen reduction reaction process and opens up a new venue for improving H_(2)O_(2)generation.

关键词： photocatalysis hydrogen peroxide oxygen reduction reaction potassium doping cyano group

Application of myxovirus resistance protein A in the etiological diagnosis of infections in adults

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World Journal of Emergency Medicine 2025年第1期16卷 35-42页

作者： Tianpeng Hu yan Li Shengtao yan Lichao Sun Rui Lian Jieqiong Yu Jie Chen Xiaoyu Liu Guoqiang zhang Department of Emergency Medicine China-Japan Friendship HospitalBeijing 100029China Graduate School Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China

BACKGROUND: Inappropriate antibiotic treatment for patients with viral infections has led to a surge in antimicrobial resistance, increasing mortality and healthcare costs. Viral and bacterial infections are often difficult to distinguish. Myxovirus resistance protein A(MxA), an essential antiviral factor induced by interferon after viral infection, holds promise for distinguishing between viral and bacterial infections. This study aimed to determine the ability of Mx A to distinguish viral from bacterial ***: We quantified MxA in 121 infected patients via dry immunofluorescence chromatography. The Kruskal-Wallis test and receiver operating characteristic(ROC) curve analysis were used to determine the diagnostic value of Mx A, either alone or in combination with C-reactive protein(CRP) or procalcitonin(PCT), in patients with viral, bacterial, or ***: The value of MxA(ng/mL) was significantly higher in patients with viral infections than in those with bacterial and co-infections(82.3 [24.5–182.9] vs. 16.4 [10.8–26.5], P<0.0001)(82.3 [24.5–182.9] vs. 28.5 [10.2–106.8], P=0.0237). The area under the curve(AUC) of the ROC curve for distinguishing between viral and bacterial infections was 0.799(95% confidence interval [95% CI] 0.696–0.903), with a sensitivity of 68.9%(95% CI 54.3%–80.5%) and specificity of 90.0%(95% CI 74.4%–96.5%) at the threshold of 50.3 ng/mL. Combining the MxA level with the CRP or PCT level improved its ability. MxA expression was low in cytomegalovirus(15.8 [9.6–47.6] ng/mL) and Epstein-Barr virus(12.9 [8.5–21.0] ng/mL) ***: Our study showed the diagnostic efficacy of Mx A in distinguishing between viral and bacterial infections, with further enhancement when it was combined with CRP or PCT. Moreover, EpsteinBarr virus and human cytomegalovirus infections did not elicit elevated Mx A expression.

关键词： Myxovirus resistance protein A Viral infections C-reactive protein Procalcitonin Biomarker

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铜／铝固-液复合研究

铸造 2014年第7期63卷 655-658页

作者：宋克兴张亚张彦敏国秀花河南科技大学材料科学与工程学院、河南省有色金属材料科学与加工技术重点实验室、有色金属共性技术河南省协同创新中心河南洛阳471023

利用压力条件下固-液复合法,在不同的铝液浇注温度下制备铜/铝复合材料,并对复合界面的结合性能和组织结构进行了研究。结果表明:在铜板预热温度400℃、单位压力1 000 MPa等其他条件相同的前提下,铝液浇注温度720℃时制备出的铜/铝复合... 详细信息

利用压力条件下固-液复合法,在不同的铝液浇注温度下制备铜/铝复合材料,并对复合界面的结合性能和组织结构进行了研究。结果表明:在铜板预热温度400℃、单位压力1 000 MPa等其他条件相同的前提下,铝液浇注温度720℃时制备出的铜/铝复合材料抗拉强度最大,约为40.07 MPa;冶金复合界面层内部又分为不同的内层,且随着浇注温度的升高,内层数增加;沿着铝基体到铜基体的方向界面层各内层相分别为α-Al+CuAl2、CuAl2、CuAl,且各内层厚度逐渐减薄;拉伸断裂易发生在含CuAl2的层。

关键词：铜铝固-液复合界面层金属间化合物

Type synthesis and mechanical performance analysis of novel coupled metamorphic mechanisms inspired by muscle–protein connections

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Science China Technological Sciences 2025年第3期68卷 381-383页

作者： Zhizhong DENG Yuling zhang Shaoze yan Division of Intelligent and Biomechanical Systems State Key Laboratory of Tribology in Advanced Equipment Department of Mechanical Engineering Tsinghua University

Deployment structures with latch capabilities have different applications in various engineering fields owing to their convenience in foldable storage,diverse configuration properties, and controllable deformation pathways [1].This latch capability is typically achieved through one of the three approaches:conventional latch designs [2], energy-dependent multistable structures [3], and metamorphic mechanisms.

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Radical rejuvenates the click-clip reaction

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Science China Chemistry 2025年第03期 803-805页

作者： Jiong Zhou Miaomiao yan Yuanxin zhang Department of Chemistry College of SciencesNortheastern University

With the widespread attention paid to the construction of functional molecular systems,chemical synthesis is increasingly pursuing the accurate and efficient combination and dissociation of molecular units to achieve specific functions [1].Carolyn ***,Morten Meldal,and *** Sharpless,winners of the 2022 Nobel Prize in Chemistry,found that click chemistry works like LEGO bricks,using the basic block to build infinitely changing shape,which allows the molecular building block to be combined quickly and efficiently [2].

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