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miRNA-34a-5p靶向CDK6表达对肺癌细胞生物学特性的影响

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生物技术 2024年第1期34卷 63-68,55页

作者：张秀维顾桂源曹斌海安市人民医院病理科江苏海安226600

[目的]分析miRNA-34a-5p靶向CDK6表达对肺癌细胞生物学特性的影响,探讨两者的作用关系。[方法]采用手术切除治疗的非小细胞肺癌患者的手术肿瘤组织样本和癌旁组织样本,对比miRNA-34a-5p、CDK6 mRNA以及CDK6蛋白表达水平;将miR-34a-5p抑... 详细信息

[目的]分析miRNA-34a-5p靶向CDK6表达对肺癌细胞生物学特性的影响,探讨两者的作用关系。[方法]采用手术切除治疗的非小细胞肺癌患者的手术肿瘤组织样本和癌旁组织样本,对比miRNA-34a-5p、CDK6 mRNA以及CDK6蛋白表达水平;将miR-34a-5p抑制物转染至肺癌A549细胞,观察其对A549细胞存活、克隆形成、凋亡、迁移侵袭的影响;并验证miR-34a-5p对CDK6的靶向调控关系。[结果]肿瘤组织中miRNA-34a-5p(0.21±0.05)显著低于癌旁组织(0.54±0.08),而CDK6 mRNA表达水平(0.76±0.07)显著高于癌旁组织(0.23±0.04)(P<0.05);抑制质粒组的miRNA-34a-5p(0.14±0.03)、CDK6 mRNA(0.94±0.12)、增殖抑制率(17.32±2.39)%、凋亡率(12.32±2.39)%、克隆形成数(103.84±7.68)个、细胞迁移数(233.21±31.02)个、细胞侵袭数(102.16±10.93)个、CDK-6(0.79±0.09)和p-AKT(0.88±0.10)水平均高于空白对照组(0.30±0.05)、(0.75±0.09)、(29.84±5.01)%、(21.23±2.85)%、(77.17±8.43)个、(121.83±20.94)个、(24.39±5.12)个、(0.45±0.07)、(0.54±0.08)和阴性对照组(0.22±0.07)、(0.83±0.14)、(30.17±4.83)%、(22.08±3.94)%、(74.39±10.95)个、(118.38±26.38)个、(26.14±4.38)个、(0.48±0.06)、(0.56±0.07)(P<0.05)。Wt-CDK6荧光素强度(0.38±0.09)显著低于Mut-CDK6(0.99±0.21),且miR-34a-5p转染后CDK-6蛋白表达(0.28±0.05)低于阴性对照(0.95±0.11),差异存在统计学意义(P<0.05)。[结论]miRNA-34a-5p可直接靶向调控CDK6表达对肺癌细胞增殖、凋亡、侵袭、转移、克隆形成等生物学特性进行调控。

关键词： miRNA-34a-5p CDK6 肺癌生物学特性

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How Physical Disturbance and Nitrogen Addition Affect the Soil Carbon Decomposition?

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Phyton-International Journal of Experimental Botany 2022年第9期91卷 2087-2097页

作者： Muhammad Junaid Nazir xiuwei zhang Daolin Du Feihai Yu Institute of Wetland Ecology&Clone Ecology/Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation Taizhou UniversityTaizhou318000China College of the Environment&Safety Engineering Jiangsu UniversityZhenjiang212013China

The decomposition of soil organic carbon(SOC)plays a critical role in regulating atmospheric CO_(2)concentrations and climate ***,the mechanisms and factors controlling SOC decomposition are still not fully ***,we conducted a 60 days incubation experiment to test the effects of physical disturbance and nitrogen(N)addition on SOC decomposition.N addition increased the concentration of NO3-by 51%in the soil,but had little effect on the concentration of NH4+.N addition inhibited SOC decomposition,but such an effect differed between disturbed and undisturbed *** disturbed and undisturbed soils,application of N decreased SOC decomposition by 37%and 15%,*** possible explanation is that extra N input suppressed microbial N mining and/or increased the stability of soil organic matter by promoting the formation of soil aggregates and incorporating part of the inorganic N into organic matter,and consequently decreased microbial mineralization of soil organic *** disturbance intensified the inhibition of N on SOC decomposition,likely because physical disturbance allowed the added N to be better exposed to soil microbes and consequently increased the availability of added *** conclude that physical disturbance and N play important roles in modulating the stability of SOC.

关键词： Soil organic matter physical disturbance microbial N mining microbial biomass carbon N availability

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液-液相分离:细胞内的调控机制和疾病发展

激光生物学报 2024年第2期33卷 97-107页

作者：王丽王拥军万兵张允雷郭忠良张秀伟南京医科大学附属江宁医院呼吸与重症医学科南京211100 南京医科大学上海东方临床医学院呼吸与危重症医学科南京211166

液-液相分离(LLPS)作为细胞内的生物-物理现象,在细胞调控、信号传导和生物过程中发挥着重要作用,近年来引起了广泛的关注。本文从LLPS的基本概念、形成机制、生理功能以及与疾病的关联等方面进行探讨,同时展望未来研究的方向和潜在应用... 详细信息

液-液相分离(LLPS)作为细胞内的生物-物理现象,在细胞调控、信号传导和生物过程中发挥着重要作用,近年来引起了广泛的关注。本文从LLPS的基本概念、形成机制、生理功能以及与疾病的关联等方面进行探讨,同时展望未来研究的方向和潜在应用。LLPS的研究为我们深入理解细胞内的调控机制和疾病发展提供了新的视角,同时也为药物开发和生物技术的创新带来了新的可能性。

关键词：液-液相分离无膜细胞器调控机制生理功能疾病发展

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肿瘤恶病质小鼠模型的建立及其相关分子机制研究进展

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国际呼吸杂志 2024年第5期44卷 598-604页

作者：高晨曦钟海林秋琪武良权杨健张允雷张秀伟南京医科大学附属江宁医院呼吸与危重症医学科南京211100

恶病质是肿瘤患者常见的临床并发症,表现为持续性体重下降、肌肉萎缩、乏力、贫血、营养不良等症状。建立肿瘤恶病质动物模型是探究其发病机制和寻找潜在治疗方法的重要策略之一。该类模型的建立有助于加深人们对恶病质发病机制的了解,... 详细信息

恶病质是肿瘤患者常见的临床并发症,表现为持续性体重下降、肌肉萎缩、乏力、贫血、营养不良等症状。建立肿瘤恶病质动物模型是探究其发病机制和寻找潜在治疗方法的重要策略之一。该类模型的建立有助于加深人们对恶病质发病机制的了解,以及肿瘤细胞与机体之间的相互作用,同时用于评估恶病质治疗药物的疗效和安全性。本文综述了肿瘤恶病质小鼠模型的建立及其相关分子机制的研究进展,并对肿瘤恶病质模型的构建和应用进行了讨论和展望。

关键词：肿瘤恶病质模型,动物发病机制

儿童用细支气管镜在肺外周病变中的初步应用(附2例报告)

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中国内镜杂志 2024年第7期30卷 89-92页

作者：武良权杨健张巧刘盼盼张秀伟杜兴冉朱颖南京医科大学附属江宁医院呼吸与危重症医学科江苏南京211103

目的探讨儿童用细支气管镜(BF-XP290)在肺外周病变(PPL)诊疗中的应用。方法应用儿童用细支气管镜(BF-XP290)对PPL进行诊断和治疗。结果儿童用细支气管镜(BF-XP290)能够以直视的形式对发现的PPL进行诊断和治疗,其不足之处能用其他技术手... 详细信息

目的探讨儿童用细支气管镜(BF-XP290)在肺外周病变(PPL)诊疗中的应用。方法应用儿童用细支气管镜(BF-XP290)对PPL进行诊断和治疗。结果儿童用细支气管镜(BF-XP290)能够以直视的形式对发现的PPL进行诊断和治疗,其不足之处能用其他技术手段克服。结论儿童用细支气管镜(BF-XP290)可以部分代替径向超声支气管镜(R-EBUS)的作用,减少医疗设备的投入,值得临床推广。

关键词：肺外周病变(PPL) 奥林巴斯细支气管镜(BF-XP290) 支气管异物

User space transformation in deep learning based recommendation

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Journal of Systems Engineering and Electronics 2020年第4期31卷 674-684页

作者： WU Caihua MA Jianchao zhang xiuwei XIE Dang Radar Non-Commissioned Officer School Air Force Early Warning AcademyWuhan 430345China

Deep learning based recommendation methods, such as the recurrent neural network based recommendation method(RNNRec) and the gated recurrent unit(GRU) based recommendation method(GRURec), are proposed to solve the problem of time heterogeneous feedback recommendation. These methods out-perform several state-of-the-art methods. However, in RNNRec and GRURec, action vectors and item vectors are shared among users. The different meanings of the same action for different users are not considered. Similarly, different user preference for the same item is also ignored. To address this problem, the models of RNNRec and GRURec are modified in this paper. In the proposed methods, action vectors and item vectors are transformed into the user space for each user firstly, and then the transformed vectors are fed into the original neural networks of RNNRec and GRURec. The transformed action vectors and item vectors represent the user specified meaning of actions and the preference for items, which makes the proposed method obtain more accurate recommendation results. The experimental results on two real-life datasets indicate that the proposed method outperforms RNNRec and GRURec as well as other state-of-the-art approaches in most cases.

关键词： recommender system collaborative filtering time heterogeneous feedback recurrent neural network gated recurrent unit(GRU) user space transformation

miR-338-3p acts as a tumor suppressor in lung squamous cell carcinoma by targeting FGFR2/FRS2

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Cancer Pathogenesis and Therapy 2023年第2期1卷 87-97页

作者： Xia Shan Cheng zhang Chunyu Li Xingchen Fan Guoxin Song Jingfeng Zhu Risheng Cao xiuwei zhang Wei Zhu Department of Respiration Jiangsu Province HospitalAnd Nanjing Medical University First Affiliated HospitalNanjingJiangsu 210000China Women&Children Central Laboratory Jiangsu Province HospitalAnd Nanjing Medical University First Affiliated HospitalNanjingJiangsu 210036China Women&Children Intensive Care Unit Jiangsu Province HospitalAnd Nanjing Medical University First Affiliated HospitalNanjingJiangsu 210036China Department of Oncology Jiangsu Province HospitalAnd Nanjing Medical University First Affiliated HospitalNanjingJiangsu 210029China Department of Pathology Jiangsu Province HospitalAnd Nanjing Medical University First Affiliated HospitalNanjingJiangsu 210029China Department of Nephrology Jiangsu Province HospitalAnd Nanjing Medical University First Affiliated HospitalNanjingJiangsu 210029China Department of Science and Technology Jiangsu Province HospitalAnd Nanjing Medical University First Affiliated HospitalNanjingJiangsu 210029China

Background:Lung cancer refers to the occurrence of malignant tumors in the lung,and squamous cell carcinoma is one of the most common pathological types of non-small cell lung *** have shown that microRNAs(miRNAs)play an important role in the occurrence,development,early diagnosis,and treatment of lung *** study aimed to explore the role and possible mechanism of MicroRNA-338-3p(miR-338-3p)in lung squamous cell carcinoma(LUSC).

关键词： miR-338-3p FGFR2

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结直肠癌患者益处发现影响因素的研究进展

中华老年多器官疾病杂志 2024年第2期23卷 149-152页

作者：刘红彦叶铖林张秀伟吕小英金培英李春湖州师范学院护理学院浙江湖州313000 湖州市中心医院护理部浙江湖州313000

本文简述了益处发现的概念和评估工具,并分别从社会人口学因素(性别、年龄、婚姻状况、家庭关系、经济水平、医保情况及受教育程度)、心理因素、疾病和治疗相关因素、应对方式和社会支持等方面综述了结直肠癌患者益处发现的影响因素。... 详细信息

本文简述了益处发现的概念和评估工具,并分别从社会人口学因素(性别、年龄、婚姻状况、家庭关系、经济水平、医保情况及受教育程度)、心理因素、疾病和治疗相关因素、应对方式和社会支持等方面综述了结直肠癌患者益处发现的影响因素。为医护人员在治疗护理癌症患者时评估其益处发现水平,引导患者认识益处发现的表现,并有针对性地帮助其进行积极心理调适提供了借鉴。

关键词：结直肠癌益处发现影响因素心理护理

Absent in melanoma 2 attenuates proliferation and migration and promotes apoptosis of human colorectal cancer cells by activating P38MAPK signaling pathway

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Oncology Research 2024年第2期32卷 353-360页

作者： ZHI zhang XIAOSONG LI YING zhang HAO ZHU ZHENGUO QIAO YANG LU xiuwei MI HUIHUA CAO GENHAI SHEN SONGBING HE Department of General Surgery Suzhou Ninth People’s HospitalSuzhou Ninth Hospital Affiliated to Soochow UniversitySuzhouChina Medical College of Nantong University NantongChina Department of Gastroenterology Suzhou Ninth People’s HospitalSuzhou Ninth Hospital Affiliated to Soochow UniversitySuzhouChina Department of General Surgery The First Affiliated Hospital of Soochow UniversitySuzhouChina Department of Oncological Surgery Kunshan Traditional Hospital Affiliated to Nanjing University of Chinese MedicineKunshanChina

Colorectal cancer(CRC)stands among the top prevalent cancers worldwide and holds a prominent position as a major contributor to cancer-related mortality *** in melanoma 2(AIM2),a constituent of the interferoninducible hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats protein family,contributes to both cancer progression and inflammasome *** this understanding,the precise biological functions and molecular mechanisms governed by AIM2 in CRC remain ***,this study endeavors to assess AIM2’s expression levels,explore its potential antitumor effects,elucidate associated cancer-related processes,and decipher the underlying signaling pathways in *** findings showed a reduced AIM2 expression in most CRC cell *** of AIM2 levels suppressed CRC cell proliferation and migration,altered cell cycle by inhibiting G1/S transition,and induced cell *** research uncovered the participation of P38 mitogen-activated protein kinase(P38MAPK)in AIM2-mediated modulation of CRC cell apoptosis and ***,our achievements distinctly underscored AIM2’s antitumor role in ***2 overexpression inhibited proliferation and migration and induced apoptosis of CRC cells via activating P38MAPK signaling pathway,indicating AIM2 as a prospective and novel therapeutic target for CRC.

关键词： Absent in melanoma 2 Proliferation Migration Apoptosis P38MAPK Colorectal cancer