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Chromosome painting of telomeric repeats reveals new evidence for genome evolution in peanut

Journal of Integrative Agriculture 2016年第11期15卷 2488-2496页

作者： DU Pei LI Li-na zhang Zhong-xin LIU Hua QIN Li HUANG Bing-yan DONG Wen-zhao TANG Feng-shou QI Zeng-jun zhang xin-you State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Industrial Crops Research Institute Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai PlainsMinistry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement College of Agriculture Henan University of Science and Technology

Interspecific hybridization is an important approach to improve cultivated peanut varieties. Cytological markers such as tandem repeats will facilitate alien gene introgression in peanut. Telomeric repeats have also been frequently used in chromosome research. Most plant telomeric repeats are（TTTAGGG）n that are mainly distributed at the chromosome ends, although interstitial telomeric repeats（ITRs） are also commonly identified. In this study, the telomeric repeat was chromosomally localized in 10 Arachis species through sequential GISH（genomic in situ hybridization） and FISH（fluorescence in situ hybridization） combined with 4＇,6-diamidino-2-phenylindole（DAPI） staining. Six ITRs were identified such as in the centromeric region of chromosome Bi5 in Arachis ipa？nsis, pericentromeric regions of chromosomes As5 in A. stenosperma, Bho7 in A. hoehnei and Av5 in A. villosa, nucleolar organizer regions of chromosomes As3 in A. stenosperma and Adi3 in A. diogoi, subtelomeric regions of chromosomes Bho9 in A. hoehnei and Adu7 in A. duranensis, and telomeric region of chromosome Es7 in A. stenophylla. The distributions of the telomeric repeat, 5S r DNA, 45 S r DNA and DAPI staining pattern provided not only ways of distinguishing different chromosomes, but also karyotypes with a higher resolution that could be used in evolutionary genome research. The distribution of telomeric repeats, 5S r DNA and 45 S r DNA sites in this study, along with inversions detected on the long arms of chromosomes Kb10 and Bho10, indicated frequent chromosomal rearrangements during evolution of Arachis species.

关键词： Arachis species inversion interstitial telomeric repeats karyotype

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Diagnosis and Treatment of Uveal Effusion Syndrome: a Case Series and Literature Review

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Chinese Medical Sciences Journal 2011年第4期26卷 231-236页

作者： Chan Wu Fang-tian Dong Hua zhang you-xin Chen Rong-ping Dai Ke Tan Department of Ophthalmology Peking Union Medical College HospitalChinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China

Objective To explore the diagnosis, classification, and management of uveal effusion syndrome (UES). Methods The clinical data of 10 patients diagnosed with UES in our hospital between 1990-2010 were extracted from hospital records and analyzed, including ophthalmologic examination, ophthalmologic ultrasonography, ultrasound biomicroscopy (UBM), fundus fluorescence angiography (FFA), indocyanine green (ICG) angiography, surgical procedures, and outcomes. Results The fundus examination of all impacted eyes showed bullous retinal detachment shifting with head position, confirmed by ultrasonography revealing retinal and choroidal detachment. UBM showed annular peripheral ciliochoroidal detachment in all cases. FFA was performed in 5 patients and revealed leopard spots without leakage from choroid into subretinal space. ICG angiograpy was performed in 3 patients and demonstrated diffused granular marked hyperfluorescence in the choroidal fluorescence in the very early phase, which increased with time and persisted until the late phase. Four eyes of 2 patients underwent full-thickness sclerectomies and 1 eye of 1 patient underwent subscleral sclerectomy, all of whom achieved reattachment of the retina without recurrence during 1-year follow-up. Conclusions Comprehensive preoperative evaluation, including ophthalmologic ultrasonography, computed tomography, and magnetic resonance imaging, is crucial for accurate classification of UES and selection of proper management strategy. Surgical treatment can achieve optimal clinical outcomes for type 1 and type 2 UES.

关键词： uveal effusion syndrome idiopathic classification sclerectomy

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Development and characterization of new allohexaploid resistant to web blotch in peanut

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Journal of Integrative Agriculture 2021年第1期20卷 55-64页

作者： WANG Si-yu LI Li-na FU Liu-yang LIU Hua QIN Li CUI Cai-hong MIAO Li-juan zhang Zhong-xin GAO Wei DONG Wen-zhao HUANG Bing-yan ZHENG Zheng TANG Feng-shou zhang xin-you DU Pei School of Life Sciences Zhengzhou UniversityZhengzhou 450001P.R.China Industrial Crops Research Institute Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-HaiPlainsMinistry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops ImprovementZhengzhou 450002P.R.China

Peanut diseases seriously threaten peanut production, creating disease-resistant materials via interspecific hybridization is an effective way to deal with this problem. In this study, the embryo of an interspecific F1 hybrid was obtained by crossing the Silihong(Slh) cultivar with Arachis duranensis(ZW55), a diploid wild species. Seedlings were generated by embryo rescue and tissue culture. A true interspecific hybrid was then confirmed by cytological methods and molecular markers. After treating seedlings with colchicine during in vitro multiplication, the established interspecific F1 hybrid produced seeds which were named as Am1210. With oligonucleotide fluorescence in situ hybridization(Oligo FISH), molecular marker evaluations, morphological and web blotch resistance characterization, we found that: 1) Am1210 was an allohexaploid between Slh and ZW55;2) the traits of spreading lateral branches, single-seeded or double-seeded pods and red seed coats were observed to be dominant compared to the erect type, multiple-seeded pods and brown seed coats;3) the web blotch resistance of Am1210 was significantly improved than that of Slh, indicating the contribution of the web blotch resistance from the wild parent A. duranensis. In addition, 69 dominant and co-dominant molecular markers were developed which could be both used to verify the hybrid in this study and to identify translocation or introgression lines with A. duranensis chromosome fragments in future studies as well.

关键词： peanut interspecific hybridization allohexaploid Oligo FISH molecular marker web blotch

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Mining and Correlation Analysis of Association Rules between Properties and Therapeutic Efficacy of Chinese Materia Medica Based on Strategy Pattern

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Chinese Medical Journal 2018年第22期131卷 2755-2757页

作者： Di-Yao Wu xin-you zhang Xiao-Ling Zhou College of Pharmacy Jiangxi University of Traditional Chinese MedicineNanchangJiangxi 330004China College of Computer Science Jiangxi University of Traditional Chinese MedicineNanchangJiangxi 330004China

To the Editor:Chinese physicians often address the combination of the properties and therapeutic efficacy of Chinese materia medica(CMM).They believe that the properties and therapeutic efficacy of traditional Chinese medicines(TCMs)should be considered as an"organic whole.""Use based on therapeutic efficacy"can lead to omission of the properties of *** also

关键词： Mining and Correlation Analysis Association Rules Properties and Therapeutic Efficacy

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组合式芯棒在共挤复合管材机头中的设计应用

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塑料工业 2012年第3期40卷 78-80页

作者：张友新新疆天业集团有限公司新疆石河子832000

介绍一种组合式芯棒在共挤双层复合塑料管材机头的模具设计应用;为了提高复合管材的密实度和物料的加工性能,对机头的结构特点进行了优化设计,在芯棒处增设了阻流和缓冲槽装置。结果表明:机头设计合理,与普通挤出管材机头相比,物料具有... 详细信息

介绍一种组合式芯棒在共挤双层复合塑料管材机头的模具设计应用;为了提高复合管材的密实度和物料的加工性能,对机头的结构特点进行了优化设计,在芯棒处增设了阻流和缓冲槽装置。结果表明:机头设计合理,与普通挤出管材机头相比,物料具有塑化性能好,挤出稳定,管材密实强度高。

关键词：组合式芯棒双层管材优化机头

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文化援疆效果分析及发展思考

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贵州民族研究 2021年第4期42卷 82-87页

作者：张新友中共新疆区委党校决策咨询部乌鲁木齐新疆830002

新的一轮对口援疆工作开展以来,我国19个援疆省市积极响应党中央的号召,深入贯彻落实三次中央新疆工作座谈会精神与第七次全国对口支援新疆工作会议精神,全面、深入、完整、准确地推进了文化援疆的工作进程,成效显著。文章以文化援疆为... 详细信息

新的一轮对口援疆工作开展以来,我国19个援疆省市积极响应党中央的号召,深入贯彻落实三次中央新疆工作座谈会精神与第七次全国对口支援新疆工作会议精神,全面、深入、完整、准确地推进了文化援疆的工作进程,成效显著。文章以文化援疆为背景,以19个省市文化援疆实践为研究内容,列举文化援疆的主要类型,进一步分析文化援疆工程伟大实践的成效,从而提出我国今后在文化援疆工程方面的相关建议与思考。

关键词：文化援疆效果分析新疆

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新疆文化产业发展水平的评价研究

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贵州民族研究 2019年第8期40卷 125-131页

作者：张新友新疆师范大学民族学与社会学学院中共新疆区委党校

文化产业作为一种新兴的产业形态,是面向未来的朝阳产业,更是清洁、无污染的绿色产业,它的发展对于一个国家(地区)经济发展方式的转变、产业结构的优化,具有十分重要的战略意义。新疆文化资源丰富多样,但由于历史和现实等原因的制约,文... 详细信息

文化产业作为一种新兴的产业形态,是面向未来的朝阳产业,更是清洁、无污染的绿色产业,它的发展对于一个国家(地区)经济发展方式的转变、产业结构的优化,具有十分重要的战略意义。新疆文化资源丰富多样,但由于历史和现实等原因的制约,文化产业的发展尚处于初始阶段。通过构建评价指标体系,对新疆文化产业发展水平进行综合评价分析。研究发现:新疆文化产业实力、产业关联、产业资源处于一般水平,产业创新处于较低水平。综合评价结果和进行深入分析,提出了有关今后新疆文化产业相应的发展看法。

关键词：新疆文化产业发展水平评价

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