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Characterization of two novel heat shock protein 70s and their transcriptional expression patterns in response to thermal stress in adult of Frankliniella occidentalis (Thysanoptera:Thripidae)

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Journal of Integrative Agriculture 2018年第5期17卷 1023-1031页

作者： QIN Jing GAO Peng zhang xiao-xiang LU Ming-xing DU Yu-zhou School of Horticulture and Plant Protection & Institute of Applied Entomology Yangzhou University

Heat shock protein 70（HSP70） is one of the most important members in the heat shock protein family, and plays important roles in the thermotolerance of insect. To explore the molecular mechanism of thermotolerance of Frankliniella occidentalis adults, the difference in the expression of HSP70s in F. occidentalis male or female adults under the thermal stress was studied under the laboratory conditions. Two full length c DNAs of HSP70s gene（Fohsc704 and Fohsc705） were cloned from F. occidentalis by using RT-PCR and RACE. The genomic sequence was demonstrated by genomic validation, and the position and size of the intron were analyzed by sequence analysis of c DNA. Real-time PCR was used to analyze the HSP70 expression patterns. The c DNA of Fohsc704 and Fohsc705 possessed 2 073 and 1 476 bp which encoded 690 and 491 amino acids（aa） with a calculated molecular weight of 75 and 54 k Da, respectively. Four introns in Fohsc704 and six introns in Fohsc705 protein were found. However, the HSP70 protein sequences in our study were ended with EKKN and GIFL, which were different from the reported Fo HSP70s. Various expression patterns of Fohsc704 and Fohsc705 were found in both genders of F. occidentalis under thermal stress. The expression of Fohsc704 and Fohsc705 reached to the highest level at –12 and –8°C in male adults, respectively, and Fohsc705 expressed the highest level at 33°C in female adults. In conclusion, HSP70s of F. occidentalis in our study are novel heat shock proteins. There were difference in expression patterns of the two hsc70s in genders of F. occidentalis, and the two HSP70s play important roles in the thermotolerance of F. occidentalis.

关键词： Frankliniella occidentalis HSP70 temperature gene cloning expression

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氧氟沙星和左氧氟沙星与DNA的相互作用研究

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光谱学与光谱分析 2010年第2期30卷 481-485页

作者：童裳伦张晓翔浙江大学环境科学研究所污染环境修复与生态健康教育部重点实验室浙江杭州310029

采用紫外光谱、荧光光谱、荧光偏振以及K3Fe(CN)6荧光猝灭实验研究了氧氟沙星(Ofloxacin,OFLX)和左氧氟沙星(Levofloxacin,L-OFLX)与小牛胸腺DNA(ctDNA)的相互作用差异性与作用模式。紫外光谱的结果表明,当向OFLX和L-OFLX溶液中加入ctDN... 详细信息

采用紫外光谱、荧光光谱、荧光偏振以及K3Fe(CN)6荧光猝灭实验研究了氧氟沙星(Ofloxacin,OFLX)和左氧氟沙星(Levofloxacin,L-OFLX)与小牛胸腺DNA(ctDNA)的相互作用差异性与作用模式。紫外光谱的结果表明,当向OFLX和L-OFLX溶液中加入ctDNA并且浓度增大时,OFLX和L-OFLX的吸收光谱都呈现略微的减色效应,但吸收峰位置没有发生偏移,L-OFLX的减色效应略强于OFLX的减色效应;从荧光光谱以及OFLX和L-OFLX的Scatchard方程,获得其键合常数分别为1.15×***-1,3.75×***-1,表明L-OFLX与ctDNA的相互作用要略强于OFLX与ctDNA的相互作用;荧光偏振实验、单双链ctDNA与药物作用实验、K3Fe(CN)6荧光猝灭实验都表明OFLX、L-OFLX与ctDNA的作用模式可能是沟槽结合。

关键词：小牛胸腺DNA 左氧氟沙星氧氟沙星相互作用光谱法

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巷道过高应力封闭老塘边沿施工实践

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煤炭技术 2009年第2期28卷 124-125页

作者：张小祥淮南矿业集团公司张集煤矿安徽淮南232174

采取架设木垛及喷注浆等加固措施,提高了在地质异常破碎老空巷道附近新掘巷道的支护效果,提高了社会及经济效益,具有广泛的推广价值。

关键词：高应力老塘边沿异常带喷注浆施工

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不同包衣材料包衣对复方芦荟颗粒吸湿性的影响

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中药材 2010年第6期33卷 979-983页

作者：张霄翔程科合肥工业大学制药工程系安徽合肥230009

目的:考察不同包衣材料羟丙基甲基纤维素(hydroxy-propyl methylcellulose,HPMC)、乙基纤维素(ethyl-cellulose,EC)、聚丙烯酸树脂(polyacrylic resin,PR)包衣对中药颗粒吸湿性的影响。方法:分别采用单一材料包衣、复膜包衣(2种或3种包... 详细信息

目的:考察不同包衣材料羟丙基甲基纤维素(hydroxy-propyl methylcellulose,HPMC)、乙基纤维素(ethyl-cellulose,EC)、聚丙烯酸树脂(polyacrylic resin,PR)包衣对中药颗粒吸湿性的影响。方法:分别采用单一材料包衣、复膜包衣(2种或3种包衣材料依次分别包衣)、混合膜包衣(2种或3种包衣材料混合后包衣)和混合膜与复膜组合包衣方法,以包衣颗粒吸湿下降百分率以及包衣颗粒中有效成分的溶出度为评价指标,通过正交试验,筛选最佳包衣方法、包衣材料及其用量。结果:混合膜与复膜组合包衣(颗粒吸湿率下降35.39%)的防潮效果比单一材料包衣(吸湿率下降≤21.82%)、复膜包衣(吸湿率下降30.28%)或混合膜包衣(吸湿率下降31.37%)好(P<0.05)。结论:混合膜与复膜组合包衣方法(混合膜组成为EC和PR,复膜组成为HPMC;先包混合膜,后包HPMC膜)显著降低了复方芦荟颗粒吸湿率,且不影响成分的溶出,从而提高了复方芦荟胶囊的稳定性,有利于该制剂生产。

关键词：吸湿性羟丙基甲基纤维素聚丙烯酸树脂乙基纤维素

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Formation and evolution of supercritical geofluid

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Science China Earth Sciences 2025年第1期68卷 39-51页

作者： Huaiwei NI Yilin xiao xiaolin XIONG Xiandong LIU Chunxiao GAO Yi-xiang CHEN Yunguo LI Wan-Cai LI Xuan GUO Yang-Yang WANG Dong-Bo TAN Li zhang State Key Laboratory of Lithospheric and Environmental Coevolution University of Science and Technology of China Deep Space Exploration Laboratory/School of Earth and Space Sciences University of Science and Technology of China Guangzhou Institute of Geochemistry Chinese Academy of Sciences School of Earth Sciences and Engineering Nanjing University State Key Lab of Superhard Materials Jilin University

In this work, we provide a comprehensive review on the formation, evolution, properties, and effects of supercritical geofluid. In Earth's interior, enhanced miscibility between H2O and silicate by the addition of special components or by the increase of pressure and temperature gives rise to supercritical geofluid with a significant amount of both H2O and silicate *** formation of supercritical geofluid in magmatic-hydrothermal systems, typified by pegmatite system, is governed by meltfluid critical curve. The formation of supercritical geofluid in metamorphic systems, typified by subducted slab, is governed by the second critical end point. Experimental results suggest that the presence of boron and fluorine in pegmatite system makes it possible to form supercritical geofluid at crustal depths, but the release of supercritical geofluid from subducted slab is withheld until almost 100 km depth. A major presence of both H2O and depolymerized structural units（monomers, dimers, etc.） endows supercritical geofluid with unique physical properties including low density, low elastic moduli, low viscosity, high diffusivity,and high electrical conductivity. Supercritical geofluid can effectively mobilize a variety of elements even including high field strength elements and heavy rare earth elements. The chemical signatures of supercritical geofluid can be inherited by metasomatized mantle and mantle-derived melts, and this could give an explanation of the oxidation of arc magmas. Phase separation of supercritical geofluid through the mechanism of spinodal decomposition leads to formation of a melt network. Multiphase fluid inclusions recovered from subduction zone rocks and pegmatites are possible relics of supercritical geofluid. Supercritical geofluid can cause electrical anomaly and low seismic velocity near the top of subducted slab, and can be linked with intermediate-focus earthquakes. Supercritical geofluid may have played a crucial role in the formation of pe

关键词： Supercritical geofluid Miscibility Subduction zone Pegmatite

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Investigation of bubbles escape behavior from low basicity mold flux for high-Mn high-Al steels using 3D X-ray microscope

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 102-110页

作者： Qiang Liu xiang Li Shen Du Ming Gao Yanbin Yin Jiongming zhang State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China

During the continuous casting process of high-Mn high-Al steels,various types of gases such as Ar need to escape through the top of the *** which,the behavior of bubbles traversing the liquid slag serves as a restrictive link,closely associated with viscosity and the thickness of liquid *** contrast to two-dimensional surface observation,three-dimensional(3D)analysis method can offer a more intuitive,accurate,and comprehensive ***,this study employs a 3D X-ray microscope(3D-XRM)to obtained spatial distribution and 3D morphological characteristics of residual bubbles in mold flux under different basicity of liquid slag,different temperatures,and different holding *** results indicate that as basicity of slag increases from 0.52 to 1.03,temperature increases from 1423 to 1573 K,the viscosity of slag decreases,the floating rate of bubbles *** addition,when holding time increases from 10 to 30 s,the bubbles floating distance increases,and the volume fraction and average equivalent sphere diameter of the bubbles solidified in the mold flux gradually *** one word,increasing the basicity,temperature,and holding time leading to an increase in the removal rate of bubbles especially for the *** findings of bubbles escape behavior provide valuable insights into optimizing low basicity mold flux for high-Mn high-Al steels.

关键词： mold flux low basicity bubbles three-dimensional X-ray microscope viscosity

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Observation of acoustic hybrid topological phases induced by the p-d orbital interactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 132-139页

作者： xiao xiang Feng Gao Yu-Gui Peng Peng Wu Zong-Lin Li Xue-Feng Zhu School of Physics and Innovation Institute Huazhong University of Science and Technology

In spinless systems,growing attention has recently been attracted to synthetic gauge fields,which redefine the fundamental crystal symmetries by utilizing the projective algebraic ***,synthetic gauge fields have been commonly investigated in single orbital systems,and explorations on hybrid orbitals remain elusive in ***,we report the experimental observation of hybrid topological phases induced by hybrid synthetic gauge flux,which is formed by the interaction between p and d orbitals embedded in acoustic *** breaking the translation symmetries of Lxand/or Ly,we unambiguously demonstrate the first-order M?bius edge states and higher-order corner *** work establishes a platform for seeking exotic topological phases induced by hybrid-orbitals interactions and initializing the framework of hybrid-orbitalsrelated topological *** applications can be anticipated in scenarios such as high-quality sensing and robust wave trapping due to the stepwise energy distribution of the hybrid topological phase.

关键词： topological insulator synthetic gauge field projective symmetry corner states

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基本医疗保险药品支付标准体系构建探讨

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中国卫生经济 2017年第4期36卷 47-49页

作者：张晓香江苏省中医院计划财务处南京210029

通过梳理我国福建省、福建省三明市和重庆市3个省市基本医疗保险药品支付标准的试点经验,指出我国应基于分类管理的思想来制定医保支付标准,合理划分支付标准组,选择适宜方法制定支付标准,建立动态调整机制,并解决差额归属及负担问题,... 详细信息

通过梳理我国福建省、福建省三明市和重庆市3个省市基本医疗保险药品支付标准的试点经验,指出我国应基于分类管理的思想来制定医保支付标准,合理划分支付标准组,选择适宜方法制定支付标准,建立动态调整机制,并解决差额归属及负担问题,以保障医保控费政策的有效实施。

关键词：基本医疗保险药品支付标准体系构建

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公立医院执行《政府会计制度》的路径探讨

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中国卫生经济 2019年第6期38卷 78-80页

作者：张晓香江苏省中医院计划财务处

通过分析公立医院机构分类及其会计制度的特点,探讨《政府会计制度》在宏观(医院会计将从传统的记账型会计向现代管理型会计转变)、中观(医院会计将从单一权责发生制向权责发生制和收付实现制相结合转变)和微观(医院财务职能将从核算与... 详细信息

通过分析公立医院机构分类及其会计制度的特点,探讨《政府会计制度》在宏观(医院会计将从传统的记账型会计向现代管理型会计转变)、中观(医院会计将从单一权责发生制向权责发生制和收付实现制相结合转变)和微观(医院财务职能将从核算与报账向分析、管理及决策转变)三方面给公立医院带来的新变化,并以此强调医院会计适应《政府会计制度》的重要性,分析公立医院实施政府会计制度存在的问题和困难,进而阐明《政府会计制度》在公立医院的实施路径。

关键词：公立医院会计制度政府会计制度

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小波包子带频域矩和遗传算法的图像隐写分析

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系统工程理论与实践 2010年第10期30卷 1864-1869页

作者：周治平张小祥惠卯卯江南大学通信与控制工程学院无锡214122

针对现有典型的通用隐写分析方法提取特征时多数对图像进行正交小波分解中的不足,提出了一种新的利用小波包分解和遗传算法的高效的隐写检测方案.首先对原灰度图像及其预测误差图像运用小波包分解生成多个子带,提取各子带系数的直方图... 详细信息

针对现有典型的通用隐写分析方法提取特征时多数对图像进行正交小波分解中的不足,提出了一种新的利用小波包分解和遗传算法的高效的隐写检测方案.首先对原灰度图像及其预测误差图像运用小波包分解生成多个子带,提取各子带系数的直方图特征函数多阶矩作为特征,将支持向量机的分类效果作为适应度函数值返回,指导遗传算法搜索最优的特征选择方案.针对Outguess、F5、MB1隐密图像与载体图所组成的混合图像库上的仿真实验结果表明:该方法比现有的隐写分析方法,平均检测率提高了约1%-17.8%.

关键词：通用隐写分析小波包分解预测误差图像遗传算法

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