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The putative NAD(P)H Nitroreductase,Rv3131,is the Probable Activating Enzyme for Metronidazole in Mycobacterium Tuberculosis

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Biomedical and Environmental Sciences 2022年第7期35卷 652-656页

作者： DONG Wen Zhu SHI Jin CHU Ping LIU Rong Mei WEN Shu An zhang ting ting PANG Yu LU Jie Department of Infectious Diseases The First People's Hospital of ZiyangZiyang 641300SichuanChina Department of Bacteriology and Immunology Beijing Chest HospitalCapital Medical UniversityBeijing Tuberculosis and Thoracic Tumor InstituteBeijing 101149China Department of Tuberculosis Beijing Chest HospitalCapital Medical UniversityBeijing 101149China Beijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck SurgeryMOE Key Laboratory of Major Diseases in ChildrenBeijing Pediatric Research InstituteBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 101149China

Tuberculosis(TB),which is caused by the intracellular pathogen,Mycobacterium tuberculosis(Mtb),remains one of the deadliest infectious diseases worldwide,accounting for 1.45 million deaths in 2018[1].Thus,there is an increasing need to develop new antibiotics or to repurpose old drugs that have the potential to shorten the current duration of therapy.Metronidazole(MTZ)is a 5-nitroimidazole antibiotic that is mainly used in the treatment of anaerobic and protozoal infections[2].The action of mechanism for metronidazole has not been fully established.As a prodrug,MTZ is inactive until taken up and reduced by anaerobic bacteria and protozoa.It is possible that the reduction of Mtz leads to the production of toxic metabolites,which bind to deoxyribonucleic acid and electron-transport proteins of microbial pathogens,thus blocking nucleic acid synthesis[3].The impressive potency of MTZ against anaerobic bacteria indicates the potential for killing Mtb under anaerobic conditions.A previous in vitro study demonstrated that MTZ has potent activity against tubercle bacilli under anaerobic conditions[4].In a non-human primate model,MTZ has been shown to prevent reactivation of latent Mtb infection,indicating the in vivo role in targeting Mtb within an anaerobic environment[5].Although there is strong evidence for the role of MTZ in affecting anaerobic bacilli,the mechanism of action has not been elucidated in Mtb considering that MTZ must be catalyzed to a nitro-free radical form with endogenous nitroreductase.To provide insight=into how MTZ is activated in anaerobic Mtb bacilli,we first characterized the nitroreductase gene,Rv3131,and identified the key residue conferring the activation of MTZ in Mtb.

关键词： Tuberculosis action latent

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The nitrate-responsive transcription factor Md NLP7 regulates callus formation by modulating auxin response

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农业科学学报:英文版 2023年第10期22卷 3022-3033页

作者： LI Tong FENG Zi-quan zhang ting-ting YOU Chun-xiang ZHOU Chao WANG Xiao-fei Apple Technology Innovation Center of Shandong Province/Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient Production/National Key Laboratory of Wheat Improvement/College of Horticulture Science and Engineering Shandong Agricultural UniversityTai’an 271018P.R.China National Key Laboratory of Wheat Improvement/College of Life Sciences Shandong Agricultural UniversityTai’an 271018P.R.China

Under appropriate culture conditions,plant cells can regenerate new organs or even whole plants.De novo organ regeneration is an excellent biological system,which usually requires additional growth regulators,including auxin and cytokinin.Nitrate is an essential nutrient element for plant vegetative and reproductive development.It has been reported that nitrate is involved in auxin biosynthesis and transport throughout the growth and development of plants.In this study,we demonstrated that the ectopic expression of the MdNLP7 transcription factor in Arabidopsis could regulate the regeneration of root explants.MdNLP7 mainly participated in the regulation of callus formation,starting with pericycle cell division,and mainly affected auxin distribution and accumulation in the regulation process.Moreover,MdNLP7 upregulated the expression of genes related to auxin biosynthesis and transport in the callus formation stage.The results demonstrated that MdNLP7 may play a role in the nitrate-modulated regeneration of root explants.Moreover,the results revealed that nitrate–auxin crosstalk is required for de novo callus initiation and clarified the mechanisms of organogenesis.

关键词： MdNLP7 callus initiation auxin nitrate pericycle shoot regeneration

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Assessing the impact of manufacturing agglomeration on environmental pollution in Beijing-Tianjin-Hebei region using spatial panel data model

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Ecological Economy 2023年第4期19卷 374-380页

作者： zhang ting-ting International Commercial Institute Tianjin Foreign Studies UniversityTianjin 300011CHINA

The Beijing-Tianjin-Hebei region is the vanguard of economic development in northern China.Its manufacturing industry is more and more developed,but environmental pollution is also more serious.Based on the data of 13 cities in Beijing-Tianjin-Hebei region from 2017 to 2021,the paper verifies the impact of manufacturing agglomeration on environmental pollution.Both manufacturing agglomeration and environmental pollution are dependent on spatial distribution.Therefore,the paper selects spatial econometric model to study.First,the spatial lag model and spatial error model are constructed,and then the spatial lag model is selected through the results of OLS regression,LM Test and Hausman test,and the empirical process is carried out.Finally,the empirical results are analyzed and the conclusion is drawn.

关键词： manufacturing agglomeration environmental pollution impact,Beijing-Tianjin-Hebei region

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“儒士”身份与正声“律曲”——元代“以曲取士”争论再认识

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艺术百家 2024年第1期40卷 85-93页

作者：张婷婷温家天上海大学上海电影学院上海200072

元代是否存在“以曲取士”,学界至今尚无定论,但仍有可诠释的空间。文章试从“戊戌选试”““曲”“词”称谓”和“以儒任吏”三个方面展开阐述,提出以曲取“士”是为了确定儒人的户籍身份,并非科举意义上的步入“仕途”。在元代以“律... 详细信息

元代是否存在“以曲取士”,学界至今尚无定论,但仍有可诠释的空间。文章试从“戊戌选试”““曲”“词”称谓”和“以儒任吏”三个方面展开阐述,提出以曲取“士”是为了确定儒人的户籍身份,并非科举意义上的步入“仕途”。在元代以“律赋”为重的风气下,遵守格律的文人“曲”被认为是与词一体的“乐府”,以词赋取士,即包括以“律曲”取士。“以曲取士”概念的再界定,或许可以为戏曲史研究提供一个新的思考视角。

关键词：元代科举元曲词赋以儒任吏

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城市人工智能产业创新链与人才链融合的多元路径研究

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软科学 2024年第6期38卷 1-6,12页

作者：张婷婷张所地胡琳娜山西财经大学管理科学与工程学院太原030006 山西财经大学工商管理学院太原030006

基于2020年中国290个地级及以上城市的截面数据,聚焦人工智能产业,首先运用改进的TO0E理论,探析了影响人工智能产业创新链与人才链融合的机理;其次构建指标体系,借助耦合协调度模型测度了创新链与人才链的融合度;最后采用模糊集定性比... 详细信息

基于2020年中国290个地级及以上城市的截面数据,聚焦人工智能产业,首先运用改进的TO0E理论,探析了影响人工智能产业创新链与人才链融合的机理;其次构建指标体系,借助耦合协调度模型测度了创新链与人才链的融合度;最后采用模糊集定性比较分析法探究了人工智能产业创新链与人才链融合的多元路径。结果发现,人工智能产业存在四条高创新链与人才链融合的路径:(1)数字技术创新-协同创新-人才综合保障政策-经济环境型;(2)数字技术创新-数字基建-协同创新型;(3)数字基建-人才综合保障政策型;(4)数字基建-财政科技支持政策型。

关键词：人工智能产业创新链人才链融合多元路径

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Neuroprotective effects of Yiqihuoxue calm wind capsule on ischemic stroke in rats

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Chinese Journal of Natural Medicines 2017年第10期15卷 758-765页

作者： zhang Jun-Xia GUO Jiao-Mei LIN Hong-Jun zhang ting-ting LI Zhen-Guo ZHOU Ji-Chun zhang Zhen-Zhong School of Pharmaceutical Science Zhengzhou University Zhengzhou 450001 China Department of Pharmacology Henan Provincial Institute of Food and Drug Control Zhengzhou 450003 China Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases of Henan Province Zhengzhou 450001 China

Stroke remains the third leading cause of death and of adult disability worldwide.Vascular occlusion,followed by ischemic cascade,leads to irreversible tissue injury.Recombinant tissue plasminogen activator is the only FDA approved drug for the current treatment of acute ischemic stroke.However,traditional Chinese medicine has a long history and rich clinical experience in the treatment and rehabilitation of ischemic stroke.Using a classical middle cerebral artery occlusion(MCAO)stroke model,we tested the effectiveness of Yiqihuoxue calm wind(YCW)capsule on neurological function,gross pathology and oxidative stress status in MCAO rats.YCW capsule(3.36 and 6.72 g·kg^(-1) of crude drug)could significantly lower Longa's score and superoxide dismutase(SOD)level,together with less necrotic cells and infarcted area.In addition to elevated MDA and downregulated i NOS expression,YCW capsule exhibited its neuroprotective effects via free radical scavenging and NO inhibition.

关键词： Yiqihuoxue calm wind capsule Ischemic stroke Middle cerebral artery occlusion Oxidative stress Nitric oxide Platelet

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氯胺酮、去甲氯胺酮、氟胺酮3个苯环利定类物质的毛发质控样品制备研究

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药物分析杂志 2024年第1期44卷 158-167页

作者：吴健美张婷婷李静陈捷毒品监测管控和禁毒关键技术公安部重点实验室公安部禁毒情报技术中心北京100193

目的:探究氯胺酮、去甲氯胺酮、氟胺酮3个苯环利定类物质毛发质控样品的制备方法和规律,分析不同制备条件和贮存条件对毛发质控样品的性能影响。方法:通过比较空白毛发(不含氯胺酮、去甲氯胺酮和氟胺酮的毛发)浸泡在不同种类的浸泡溶剂... 详细信息

目的:探究氯胺酮、去甲氯胺酮、氟胺酮3个苯环利定类物质毛发质控样品的制备方法和规律,分析不同制备条件和贮存条件对毛发质控样品的性能影响。方法:通过比较空白毛发(不含氯胺酮、去甲氯胺酮和氟胺酮的毛发)浸泡在不同种类的浸泡溶剂、不同目标物(氯胺酮、去甲氯胺酮、氟胺酮)浓度、不同酸度的浸泡试液和不同空白毛发浸泡量等条件下制备的毛发质控样品中目标物含量,系统分析了不同制备条件对毛发质控样品性能的影响,并按照研究规律采用乙腈-DMSO(1∶1,含0.02 mol·L-1盐酸)为浸泡溶液,将空白毛发浸泡不同时间段制备含量覆盖面较广的毛发质控样品;采用高效液相色谱-质谱联用仪,应用BEH C18(100 mm×2.1 mm,1.7μm)色谱柱,在柱温40℃,以0.1%(v/v)甲酸水溶液(A)-0.1%(v/v)甲酸乙腈溶液(B)为流动相,梯度洗脱(0~9 min,5%B→100%B;9~11 min,100%B;11~11.1 min,100%B→5%B;11.1~13 min,5%B),流速0.4 mL·min-1,进样量1μL的色谱条件下测定毛发质控样品中目标物含量。同时考察了1 cm段和粉末(冷冻研磨)2种状态毛发质控样品的均匀性、不同贮存条件下(温度、湿度、光照等条件)短期和长期稳定性。结果:确定了不同制备条件对氯胺酮等3种苯环利定类物质毛发质控样品的影响规律,发现浸泡溶液种类、浓度、酸度对毛发质控样品性能有较明显的影响,浸泡量因素对空白毛发几乎没有影响。制备的高、中、低含量的氯胺酮等3种物质的毛发质控样品,在1 cm段状态和粉末状态下均有较好的均匀性,按照F检验法计算各样品的F值均

关键词：毛发质控样品氯胺酮去甲氯胺酮氟胺酮均匀性稳定性制备条件贮存条件

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回收微塑料制备改性沥青混凝土及其性能研究

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塑料科技 2024年第1期52卷 28-32页

作者：张婷婷茹沛泽信阳农林学院河南信阳464000 河南水投申城四水同治工程管理有限公司河南信阳464000

为了提高沥青混凝土的综合性能,利用废旧聚丙烯(PP)微塑料改性基质沥青,制备AC-13沥青混凝土,研究沥青及沥青混凝土的性能。结果表明:加入微塑料提高了改性沥青的抗变形能力和耐高温稳定性,也提高了沥青混凝土的力学性能、高温稳定性、... 详细信息

为了提高沥青混凝土的综合性能,利用废旧聚丙烯(PP)微塑料改性基质沥青,制备AC-13沥青混凝土,研究沥青及沥青混凝土的性能。结果表明:加入微塑料提高了改性沥青的抗变形能力和耐高温稳定性,也提高了沥青混凝土的力学性能、高温稳定性、低温稳定性和水稳定性。微塑料掺量为4%时,与基质沥青相比,改性沥青的25℃针入度降低20%,软化点提高12℃。微塑料掺量为4%时,与基质沥青混凝土相比,改性沥青混凝土的浸水马歇尔残留度(MS0)和冻融劈裂抗拉强度比(TSR)分别提高11.6%和12.8%,动稳定度提高86%,车辙深度降低67.0%,空隙率(VV)降低14.6%,稳定度(MS)和饱和度(VFA)分别提高21.1%和7.8%,低温弯曲破坏应变提高35.6%。

关键词：聚丙烯微塑料改性沥青混凝土力学性能高温稳定性低温稳定性

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T29C genotype polymorphism of estrogen receptor alpha is associated with initial response to interferon-alpha therapy in chronic hepatitis B patients

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Hepatobiliary & Pancreatic Diseases International 2010年第3期9卷 275-279页

作者： zhang, ting-ting zhang, Zhen-Hua Gao, Yu-Feng zhang, Ya-Fei Yang, Dong-Liang Li, Xu Anhui Med Coll Affiliated Hosp 1 Dept Infect Dis Hefei 230022 Peoples R China Huazhong Univ Sci & Technol Tongji Med Coll Tongji Hosp Div Clin Immunol Wuhan 430030 Peoples R China

BACKGROUND: Virological clearance, delayed progression to cirrhosis or liver cancer, and increased survival are the long-term goals of antiviral therapy in chronic hepatitis B patients. Identification of host factors correlated with therapeutic response may contribute greatly to individual treatment. This study aimed at investigating whether T29C genotype polymorphism of estrogen receptor alpha (ESR1) is associated with the initial response to interferon-alpha (IFN-alpha) therapy in chronic hepatitis B patients. METHODS: The initial responses of 100 patients to IFN-alpha therapy were evaluated and compared by classifying them into three groups according to T29C genotype polymorphism of ESR1: T/T, TIC, and C/C genotype groups. Polymerase chain reaction-restriction fragment length polymorphism was used to analyze the genotype polymorphism in T29C. RESULTS: The frequency of initially combined response was markedly higher in both the T/T and TIC groups than in the C/C group (Z=10.326, P=0.006 and Z=26.247, P=0.000, respectively). In addition, the initial virological response was higher in the T/T and T/C groups than the C/C group (chi(2)=5.674, P=0.017 and chi(2)=4.980, P=0.026, respectively). In 78 initially HBeAg-positive patients, however, the frequency of initial e-antigen disappearance or seroconversion among the T/T, T/C, and C/C genotype groups was 34.15%, 27.78% and 15.79%, respectively, which were not significantly different. CONCLUSION. The T29C genotype polymorphism of ESR1 is associated with the initial response to IFN-alpha in patients with chronic hepatitis B, and might be a significant marker for predicting the initial response to IFN-alpha, at least in this study population. (Hepatobiliary Pancreat Dis Int 2010; 9: 275-279)

关键词： estrogen receptor polymorphism chronic hepatitis B initial response interferon-alpha

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非国有股东委派董事会影响国有企业分红水平吗?

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经济问题 2024年第6期 68-75页

作者：徐高彦王雅静张婷婷河海大学商学院江苏南京211100

现金分红是回报投资者的重要方式,对于构建高效有序的资本市场至关重要。混合所有制改革后,非国有股东进驻国有企业并取得董事会权力有助于改善治理行为,这能否提升国有企业的现金分红水平呢?基于上述问题,将非国有股东董事会权力与国... 详细信息

现金分红是回报投资者的重要方式,对于构建高效有序的资本市场至关重要。混合所有制改革后,非国有股东进驻国有企业并取得董事会权力有助于改善治理行为,这能否提升国有企业的现金分红水平呢?基于上述问题,将非国有股东董事会权力与国有企业分红纳入同一解释模型,并进行实证检验。研究发现,非国有股东董事会权力的取得能够显著提升分红水平;当派出超额董事时,分红水平将进一步提升;机制分析表明,这一作用主要是通过减轻国有企业政策负担和降低代理成本得以实现;异质性分析发现,该结果在地方国有企业和实际控制人权力较大的条件下更为显著。

关键词：国有企业混合所有制改革非国有股东分红水平

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