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地球科学进展 2019年第11期34卷 1189-1201页

作者：刘羽王军李慧徐奴文张士秀周志祥王书兵蒲生彦吴国灿董紫君国家自然科学基金委员会地球科学部

着重分析了2019年度环境地球科学学科各类项目申请受理、评议和资助情况,并指出了申请和评议过程中暴露的问题;总结了2018年底该学科项目结题完成情况以及主要学科方向取得的研究进展。

关键词：环境地球科学项目评审资助成果

The changes in the geographical patterns of China’s tourism in 1978–2018:Characteristics and underlying factors

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Journal of Geographical Sciences 2020年第3期30卷 487-507页

作者： zhang Chengming WENG shixiu BAO Jigang School of Geography and Planning Sun Yat-sen UniversityGuangzhou 510275China School of Tourism Management Sun Yat-sen UniversityGuangzhou 510275China Center for Tourism Planning&Research Sun Yat-sen UniversityGuangzhou 510275China

Since the reform and opening-up policy launched in 1978,the number of inbound tourists increased from 1.8 million in 1978 to 139.5 million in 2017,and that of domestic tourists increased from 344 million in 1991 to 5 billion in *** article conducts research on how the geographical pattern of China’s tourism has evolved in the last four decades on the national-scale and regional-scale,for rare studies before could focus on such an extended date and utilize inbound and domestic tourism data *** on viable datasets and multiple vibrant data analysis approaches(including the Gini coefficient,primacy index analysis,hot spot analysis and Pearson correlation analysis),this article unpacks triple vital realities.(1)The overall geographical pattern of China’s tourism development can arguably summarize as“high in the eastern and low in the western part,high in the southern and low in the northern part.”Meanwhile,China’s inbound tourism has long shown a pattern of polarized distribution;While,domestic tourism has experienced a shift from the polarized distribution to the equilibrium distribution.(2)According to the features and characteristics,China's tourism development can be divided into four *** are precisely the initial modern tourism stage(1978–1988),the domestic tourism cultivating stage(1989–1996),the rapid development stage(1997–2007)and the new normal stage(2008–present).(3)This article also identified multiple factors underlying the inbound and domestic tourism development in China,including policies,management systems,tourism demand,tourist attractions,economic level,consumption level,industrial development,investment status,traffic conditions,accommodation services,intermediary services and degree of openness.

关键词： reform and opening-up tourism development spatial pattern development stage influencing factor China

典型黑土带玉米农田土壤微生物群落地理分布及驱动因素

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土壤与作物 2024年第1期13卷 1-12页

作者：逄梦璇刘红文韩旭张梦亭刘萍高燕张淼张士秀梁爱珍中国科学院东北地理与农业生态研究所黑土区农业生态重点实验室吉林长春130102 北华大学林学院吉林吉林132013 中国科学院大学北京100049

探究东北黑土带土壤微生物群落的地理分布格局及其驱动因子,对于有效和可持续地管理这一宝贵的土地资源具有重要的学术价值。本文以东北典型黑土带玉米农田的耕层土壤为研究对象,采集了来自黑龙江省、吉林省和辽宁省24个样点72个土壤样... 详细信息

探究东北黑土带土壤微生物群落的地理分布格局及其驱动因子,对于有效和可持续地管理这一宝贵的土地资源具有重要的学术价值。本文以东北典型黑土带玉米农田的耕层土壤为研究对象,采集了来自黑龙江省、吉林省和辽宁省24个样点72个土壤样本。利用磷脂脂肪酸(Phospholipidfattyacid,PLFA)技术定量描述土壤微生物群落结构,运用随机森林和层次分割分析等机械学习算法深入解析环境因子对微生物地理分布的相对贡献。研究发现,典型黑土带玉米农田土壤总微生物和各微生物类群的生物量呈现随纬度自南向北增加的空间分布格局;土壤有机碳(SOC)和土壤全氮(TN)不仅是驱动微生物生物量纬度分布格局的主要因素,也是影响微生物群落组成最重要的因素。SOC和TN还与其他环境因子,特别是与土壤粘粒含量(CC)之间存在耦合关系,共同影响东北黑土带土壤微生物群落组成。

关键词：生物地理典型黑土微生物生物量土壤有机碳层次分割

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海水养殖动物致病性(Pathogenicity)鳗弧菌(Vibro anguillarum)的研究综述

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现代渔业信息 2007年第1期22卷 16-19,22页

作者：张新中张世秀李海平谢珍玉周永灿海南大学海洋学院中国海南省海口市570228

鳗弧菌(Vibrioanguillarum)广泛存在于沿岸海水、沉积物和海洋动物体中,是海水鱼、虾、贝类的一种常见的细菌性病原。随着海水养殖业的日益发展,致病性鳗弧菌对养殖动物的危害也越来越严重。主要对鳗弧菌的生物学性状、检测技术、鳗弧... 详细信息

鳗弧菌(Vibrioanguillarum)广泛存在于沿岸海水、沉积物和海洋动物体中,是海水鱼、虾、贝类的一种常见的细菌性病原。随着海水养殖业的日益发展,致病性鳗弧菌对养殖动物的危害也越来越严重。主要对鳗弧菌的生物学性状、检测技术、鳗弧菌病症状和病理变化及海水养殖动物鳗弧菌病的防治作了简单回顾,并对鳗弧菌病的诊断、防治方法及其研究方向进行了探讨和展望。

关键词：海水养殖鳗弧菌生物学性状症状及病理变化检测防治

Ordered mesoporous carbon spheres assisted Ru nanoclusters/RuO_(2) with redistribution of charge density for efficient CO_(2) methanation in a novel H2/CO_(2)fuel cell

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Journal of Energy Chemistry 2022年第9期31卷 116-124,I0004页

作者： Yan Liu Tao zhang Chao Deng shixiu Cao Xin Dai Shengwu Guo Yuanzhen Chen Qiang Tan Haiyan Zhu Sheng zhang Yongning Liu State Key Laboratory for Mechanical Behavior of Materials Xi’an Jiaotong UniversityXi’an 710049ShaanxiChina Department of Chemical and Biochemical Engineering RutgersThe State University of New JerseyPiscataway 08854New JerseyUnited States Chongqing University Key Laboratory of Micro/Nano Materials Engineering and Technology Chongqing University of Arts and SciencesChongqing 402160China Institute of Modern Physics Northwest UniversityShaanxi Key Laboratory for Theoretical Physics FrontiersXi’an 710069ShaanxiChina Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

Efficiently reducing carbon dioxide(CO_(2))into carbon chemicals and fuels is highly desirable due to the rapid growth of atmospheric CO_(2)*** prior work,we described a unique H/CO_(2)fuel cell driven by low-valued waste heat,which not only CO_(2)nverts CO_(2)to methane(CH_(4))but also outputs electrical energy,yet the CO_(2)reduction rate needs to be urgently ***,a novel Ru-RuOcatalyst with heterostructure was grafted on mesoporous carbon spheres by in situ partially reducing RuOinto ultrasmall Ru clusters(~1 nm),in which heteroatom-doped carbon spheres as a matrix with excellent CO_(2)nductivity and abundant pores can not only easily CO_(2)nfine the formation of Ru nanocluster but also are beneficial to the exposed active sites of Ru CO_(2)mplex and the mass ***_(2)mpared to pure RuOnanoparticles supported on carbon spheres,our CO_(2)mposite catalyst boosts the CO_(2) nversion rate by more than 5-fold,reaching a value of 382.7μmol gcat.h-1at 170℃.Moreover,a decent output power density of 2.92 W mwas obtained from this H2/CO_(2)fuel cell using Ru-RuOembedded carbon spheres as a cathode *** Ru-RuOheterostructure can modify the adsorption energy of CO_(2)and induce the redistribution of charge density,thus boosting CO_(2)reduction *** work not only offers an efficient catalyst for this novel H_(2)/CO_(2)fuel cell but also presents a facile method to prepare Ru nanoclusters.

关键词： CO_(2)methanation Fuel cell Generating electricity Ru nanocluster/RuO_(2)hybrid Ordered mesoporous carbon spheres

A review on marine collagen:sources,extraction methods,colloids properties,and food applications

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Collagen and Leather 2024年第1期6卷 1-27页

作者： Shahzad Farooq Muhammad Ijaz Ahmad Shijie Zheng Usman Ali Yang Li Cui shixiu Hui zhang Jiaxing Institute of Future Food Jiaxing 314050China College of Biosystems Engineering and Food Science Zhejiang Key Laboratory for Agro-Food ProcessingZhejiang UniversityHangzhou 310058China

The growing interest in valorizing industrial by-products has led researchers to focus on exploring different sources and optimizing collagen extraction conditions over the past *** bovine hide,cattle bones,pork,and pig skins remain the most abundant collagen sources,there is a growing trend in the industrial utilization of collagen from non-mammalian *** review explores alternative marine collagen sourcesand summarizes emerging trends in collagen recovery from marine sources,with a particular focus on environmentally friendly ***,this review covers the colloidal structure-forming properties of marine collagens,including foam,film,gel,and emulsion *** also highlights the potential and important applications of marine collagen in various food *** on the currently reported marine sources,collagens extracted from fish,jellyfish,and sea cucumbers were found to have the highest yield and mostly comprised type-l collagen,while crustaceans and mollusks yielded lower percentages of *** extraction techniques isolate collagen based on acetic acid and pepsin treatment,but they come with drawbacks such as being time-consuming,causing sample destruction,and using ***,marine collagen extracted using conventional methods assisted with ultrasonication resulted in higher yields and strengthened the triple-stranded helical ***,an increasing number of new applications have been found in the food industry for marine collagens,such as biodegradable film-forming materials,colloid stabilizers,foaming agents,and micro-encapsulating ***,collagen is a modern foodstuff and is extensively used in the beverage,dairy,and meat industries to increase the stability,consistency,and elasticity of products.

关键词： Marine collagen Jellyfish Sea cucumber Colloid stabilizer Edible film Dairy product

Expression and antimicrobial activity of the recombinant bovine lactoferricin in Pichia pastoris

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Synthetic and Systems Biotechnology 2024年第1期9卷 26-32页

作者： Xueqin Lv Yuting zhang Lingrui Wang shixiu Cui Yanfeng Liu Jianghua Li Guocheng Du Long Liu Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationJiangnan UniversityWuxi 214122China Science Center for Future Foods Jiangnan UniversityWuxi 214122China Yixing Institute of Food Biotechnology Co. LtdYixing 214200China Jiaxing Institute of Future Food Jiaxing314015China Food Laboratory of Zhongyuan Jiangnan UniversityWuxi 214122China

Lactoferricin,a multifunctional peptide located in the N-terminal region of lactoferrin,has a broad-spectrum bacteriostatic *** is a promising candidate as a food additive and immune fortification agent and does not have the risks associated with drug residues and drug ***,we performed promoter and host cell screening to achieve the recombinant expression of lactoferricin in Pichia pastoris,showing an initial titer of 19.5 mg/L in *** X-33 using PAOX1 ***,we constructed a 0030-α hybrid signal peptide by fusing the 0030 signal peptide with the pro-sequence of α-factor secretory signal *** further increased the production of lactoferricin,with a titer of 28.8 mg/L in the fermentation supernatant in the shaking ***,we increased the expression of lactoferricin by fusing it with anionic antioxidant *** neutralization of positive charges yielded a titer of 55.3 mg/L in the shaking flask,and a highest titer of 193.9 mg/L in a 3-L *** antimicrobial activity analysis showed that recombinant-expressed lactoferricin exhibited potent antibacterial activity against Escherichia coli,Bacillus subtilis,and Staphylococcus *** study provides a reference for the construction of microbial cell factories capable of efficiently synthesizing antimicrobial peptides.

关键词： Antimicrobial peptide Lactoferricin Recombinant expression Pichia pastoris

不同生长年份牛大力根淀粉和蔗糖代谢途径转录组分析

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热带作物学报 2025年第1期46卷 21-34页

作者：羊青顾学金王清隆张旻晏小霞汤欢王祝年冯世秀王茂媛中国热带农业科学院热带作物品种资源研究所/农业农村部中药材生物学与栽培重点实验室/农业农村部热带农业野生植物基因资源鉴定评价中心/海南省热带药用植物工程研究中心海南海口571101 云南农业大学热带作物学院云南普洱665001 深圳市中国科学院仙湖植物园/深圳市南亚热带植物多样性重点实验室广东深圳518004

牛大力是一种重要的多年生药食同源植物,其主要成分为淀粉和糖类。为了探究不同生长年份牛大力根淀粉和糖类物质合成的规律及其内在基因调控网络,本研究以3年生(NG3)、7年生(NG7)、10年生(NG10)和15年生(NG15)4个生长年份的牛大力根为... 详细信息

牛大力是一种重要的多年生药食同源植物,其主要成分为淀粉和糖类。为了探究不同生长年份牛大力根淀粉和糖类物质合成的规律及其内在基因调控网络,本研究以3年生(NG3)、7年生(NG7)、10年生(NG10)和15年生(NG15)4个生长年份的牛大力根为研究对象,测定总多糖、淀粉和蔗糖的含量,并完成转录组测序,筛选分析不同年份间牛大力根的差异表达基因(DEGs),重点解析淀粉和蔗糖代谢途径DEGs的功能。糖类和淀粉物质测定结果表明,随着年份的增长,总多糖与淀粉含量均呈现增加趋势,生长至15年时的含量最高,而蔗糖含量在3年时最高,随后呈现下降趋势。转录组测序发现,随着生长年份跨度增加,NG3vs NG7、NG3 vsNG10和NG3 vs NG15对比的DEGs总数呈上升趋势,分别为526、1848和1937个。进一步挖掘淀粉和蔗糖代谢途径中的DEGs,共有62个DEGs在3个比较组中差异表达,其中,蔗糖合成基因的表达量随年份增加而下降,淀粉合成基因随年份的增加较淀粉降解基因占主导,纤维素降解酶随年份增加表达量降低。最终筛选出5个淀粉和蔗糖代谢途径的关键酶基因进行荧光定量PCR验证,其表达变化趋势与转录组测序结果基本一致。研究结果表明,牛大力在3~7年时处于生长活跃期,以蔗糖、纤维素和淀粉的合成为主,促进根部快速膨大发育。生长至7年时,以淀粉的积累、蔗糖的分解和纤维素的降解为特征,进入生长平稳期。本研究对于牛大力根膨大特性的研究、高成薯性牛大力种质创新和科学采收都有积极的指导意义。

关键词：牛大力根生长年份淀粉蔗糖代谢转录组分析

保护性耕作对东北黑土地区土壤团聚体和团聚体胶结物质的影响研究

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科技创新导报 2016年第14期13卷 178-178页

作者：李琪张士秀梁文举中国科学院沈阳应用生态研究所

耕作强烈地影响土壤团聚体的稳定性,在一定空间尺度范围内涉及到许多胶结物质的相互作用机理。为了更好地了解土壤胶结物质促进土壤团聚体团聚过程,在东北黑土地区开展了有关三种不同耕作方式(免耕、垄作、常规耕作)对土壤团聚体和土壤... 详细信息

耕作强烈地影响土壤团聚体的稳定性,在一定空间尺度范围内涉及到许多胶结物质的相互作用机理。为了更好地了解土壤胶结物质促进土壤团聚体团聚过程,在东北黑土地区开展了有关三种不同耕作方式(免耕、垄作、常规耕作)对土壤团聚体和土壤团聚体胶结物质(有机碳、微生物量、土壤球囊霉素相关蛋白)的影响试验研究。研究结果表明,与常规耕作相比,垄作提高了所有粒级团聚体内有机碳含量,而免耕只提高了大团聚体内有机碳含量。在免耕和垄作条件下,全土和各个粒级土壤团聚体中的微生物生物量和土壤球囊霉素相关蛋白含量显著高于常规耕作。在四个粒级土壤团聚体中,大团聚体和微团聚体内土壤有机碳、微生物生物量以及易提取球囊霉素相关蛋白含量较高,而土壤总球囊霉素相关蛋白则均匀地分布在各个粒级团聚体内。应用结构方程模型揭示土壤有机碳、微生物生物量及球囊霉素相关蛋白含量能够解释79%的土壤团聚过程。土壤有机碳是通过微生物量碳和微生物量氮而间接影响土壤团聚体稳定性。在垄作和免耕系统中,土壤微生物生物量和球囊霉素相关蛋白是提高土壤团聚体稳定性的重要驱动因子。该研究结果表明,在东北黑土地区实施保护性耕作(如垄作和免耕)措施有助于改善土壤结构,尤其是能显著增强黑土团聚体团的稳定性。

关键词：保护性耕作土壤团聚体土壤有机碳微生物生物量土壤球囊霉素相关蛋白结构方程模型

1978年改革开放以来中国旅游业发展的地理格局

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地理学报 2019年第10期74卷 1980-2000页

作者：张城铭翁时秀保继刚中山大学地理科学与规划学院广州510275 中山大学旅游学院广州510275 中山大学旅游发展与规划研究中心广州510275

运用基尼系数、首位度分析、热点分析和Pearson相关分析,从全国和地区两种尺度对1978年改革开放40年来中国旅游业发展格局进行了研究。分析发现:①旅游业发展格局的整体特征是东高西低,南高北低;入境旅游长期呈现极化分布的特征,广东是... 详细信息

运用基尼系数、首位度分析、热点分析和Pearson相关分析,从全国和地区两种尺度对1978年改革开放40年来中国旅游业发展格局进行了研究。分析发现:①旅游业发展格局的整体特征是东高西低,南高北低;入境旅游长期呈现极化分布的特征,广东是入境旅游的“极核”,京—沪处于第二梯队,甘—新—青—藏—宁发展滞后;国内旅游经历了极化分布到均衡分布的过程。②旅游业发展格局阶段性特征:现代旅游起始阶段——入境旅游极化严重,广东地位举足轻重;国内旅游培育阶段——入境旅游空间结构未变(广东第一,北京第二,上海第三),国内旅游总体极化分布;旅游大发展阶段——北京入境旅游地位逐渐被上海取代,国内旅游总体均衡分布;旅游新常态阶段——入境旅游空间结构改善,国内旅游第一层级省份明显增加,出境旅游稳步快速增长,北京、上海引领出境旅游人均消费。③影响中国旅游业发展的因素比较多元:政策、管理制度、旅游需求、旅游吸引物、经济水平、消费水平、产业发展、投资状况、交通条件、食宿服务、中介服务和对外开放程度等因素对中国国内旅游和入境旅游均有不同程度的影响。

关键词：改革开放旅游业发展格局旅游发展阶段影响因素中国