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Zoological Research 2022年第4期43卷 523-527页

作者： Yi-Xin Chen Yang Yu Cheng Li Zhi-Shu Xiao Guo-Wei Zhou Zhong-Jian zhang Xin-Wen Wang Zuo-Fu Xiang jiang Chang Ming Li institute of Evolutionary Ecology and Conservation Biology Central South University of Forestry&TechnologyChangshaHunan 410004China College of Life Science and Technology Central South University of Forestry&TechnologyChangshaHunan 410004China state key Lab of integrated management of pest insects and rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101China Lushui Bureau of Mt.Gaoligong National Nature Reserve LiukuYunnan 673229China College of Forestry Central South University of Forestry&TechnologyChangshaHunan 410004China state key laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China key Lab of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingYunnan 650223China

DEAR EDITOR,We examined the distribution,population,and conservation status of the critically endangered Myanmar or black snub-nosed monkey(Rhinopithecus strykeri)via field surveys over 26 months(2019-2021)in the Pianma region of the china-Myanmar *** to previous reports,we only identified one group in the region,which was a cross-border group occupying a multi-year home range of 51.50-57.02 km^(2).The current group size was much larger(155-160 individuals)than that in 2012-2014(ca 100 individuals),and the group appeared to be ***,confirmed poaching,mining,and transboundary forest fires on the Myanmar side of the border threaten their survival.

关键词： black boundary Myanmar

Exaggerated male pheromones in rats may increase predation cost

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Current zoology 2016年第5期62卷 431-437页

作者： Yao-Hua zhang Hong-Chun LIANG Hong-Ling Guo Jian-Xu zhang state key laboratory of integrated management of pest insects and rodents in agriculture Institute of Zoology Chinese Academy of Sciences Beijing 100101 China College of Horticulture and Landscape Tianjin Agricultura University Tianjin 300384 Chin CCollege of Life sciences University of Chinese Academy of Sciences Beijing 100049 China

Male animals with more conspicuous visual and acoustic signals increase their mating success, but also increase the risk of being attacked by eavesdropping predators. In rodents, males with richer sex pheromones often have higher attractiveness to females, but whether or not the males are also at higher predation risk is poorly known. Here, we used 2 laboratory inbred strains of the rat Rattus norvegicus, Brown Norway （BN） and Lewis （LEW）, and wild-captured rats as odor donors to assess the relationship between the pheromone levels in male rats and attractiveness to domes- tic cats Felis catus. LEW rats had significantly higher levels of male pheromones （e.g., 4-heptanone, 2-heptanone, and 9-hydroxy-2-nonanone） than BN rats. Simultaneously, wild-captured male rats were selectively assigned to 2 groups （HIGH or LOW） based on pheromone content as determined by gas chromatography-mass spectrometry （GC-MS）. Binary choice tests were carried out during the night in the test room. We found that cats spent more time investigating male bedding and urine of LEW rats than the counterpart of BN rats. Likewise, cats also preferred bedding and urine odor of the HIGH wild rats compared with the counterparts of LOW wild rats. Adding synthetic ana- logs of the 3 pheromone ketones into the urine of either BN rats or LOW wild rats significantly increased their attractiveness to cats. Our data suggest that the rats with exaggerated male phero- mones more strongly attracted predators and thus as a consequence may suffer from elevated pre- dation risk.

关键词： olfactory preference pheromone predator risk sexual ornament.

Cuticular protein LmTwdll is involved in molt development of the migratory locust

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Insect Science 2016年第4期23卷 520-530页

作者： Tian-Qi Song Mei-Ling Yang Yan-Li Wang Qing Liu Hui-Min Wang Jie zhang Tao Li Research institute of Applied Biology Shanxi University Taiyuan and State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing China beijing institutes of Life Science Chinese Academy of Sciences Beijing China

The cuticle, an essential structure for insects, is produced from cuticular proteins and chitin via a series of biochemical reactions. Tweedle genes are important members of the cuticular protein family and have four conserved motifs binding to chitin. Tweedle family genes have been found to play a profound effect on cuticle development. Here, we report that the cuticular protein gene LmTwdll ofLocusta migratoria belongs to the Tweedle family. In situ hybridization showed that LmTwdll is localized to epidermal cells of the cuticle. The expression patterns of LmTwdll showed low expression in the cuticle during the early and middle stages of the fifth-instar nymphs; in contrast, its expression rapidly increased in the late stages of fifth-instar nymphs. We performed RNA interference to examine the function of LmTwdll in locusts. Silencing of LmTwdll resulted in high mortality during the molting process before the next stage. Also, the epicuticle of nymphs failed to molt, tended to be thinner and the arrangement of chitin in the procuticle appeared to be disordered compare to the control group. These results demonstrate that LmTwdl! plays a critical role in molting, which contributes to a better understanding of the distinct functions of the Tweedle family in locusts.

关键词： cuticular protein Lm Twdll Loeusta migratoria molting RNA interference

Genomic and transcriptomic analysis unveils population evolution and development of pesticide resistance in fall armyworm Spodoptera frugiperda

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Protein & Cell 2022年第7期13卷 513-531页

作者： Furong Gui Tianming Lan Yue Zhao Wei Guo Yang Dong Dongming Fang Huan Liu Haimeng Li Hongli Wang Ruoshi Hao Xiaofang Cheng Yahong Li Pengcheng Yang Sunil Kumar Sahu yaping Chen Le Cheng Shuqi He Ping Liu Guangyi Fan Haorong Lu Guohai Hu Wei Dong Bin Chen Yuan jiang Yongwei zhang Hanhong Xu Fei Lin Bernard Slippers Alisa Postma Matthew Jackson Birhan Addisie Abate Kassahun Tesfaye Aschalew Lemma Demie Meseret Destaw Bayeleygne Dawit Tesfaye Degefu Feng Chen Paul K.Kuria Zachary M.Kinyua Tong-Xian Liu Huanming Yang Fangneng Huang Xin Liu Jun Sheng Le Kang state key laboratory for Conservation and Utilization of Bioresources in Yunnan Yunnan Agricultural UniversityKunming 650201China state key laboratory of Agricultural Genomics BGI-ShenzhenShenzhen 518083China state key laboratory of integrated management of pest insects and rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101China beijing institutes of Life Science Chinese Academy of SciencesBeijing 100101China MGI BGI-ShenzhenShenzhen 518083China BGI-Qingdao BGI-ShenzhenQingdao 266555China BGI-Yunnan No.389 Haiyuan RoadHigh-tech Development ZoneKunming 650106China china National GeneBank Jinsha RoadDapeng New DistrictShenzhen 518120China Department of Biology University of Copenhagen2100 CopenhagenDenmark Guangdong Provincial key laboratory of Genome Read and Write BGI-ShenzhenShenzhen 518120China Guangdong Provincial Academician Workstation of BGI Synthetic Genomics BGI-ShenzhenShenzhen 518120China Yunnan Plateau Characteristic agriculture Industry Research institute Kunming 650201China College of Plant Health and Medicine Qingdao Agricultural UniversityQingdao 266109China Department of Entomology Louisiana State University Agricultural CenterBaton RougeLA 70803USA CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100101China Yunnan Plant Protection and Quarantine Station Kunming 650034China state key laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural UniversityGuangzhou 510642China BGI-Americas One Broadway14th FloorCambridgeMA 02142USA Departme nt of Biochemistry Gen etics and MicrobiologyForestry and Agricultural Biotech no logy InstituteUn iversity of PretoriaPretoriaSouth Africa Ethiopian Biotechnology institute Addis AbabaEthiopia College of Natural Science Addis Ababa UniversityAddis AbabaEthiopia Melkassa Agricultural Research Center Ethiopian Institute of Agricultural ResearchMelkassaAddis AbabaEthiopia Kenya Agricultural and Livestock Res

The fall armyworm(FAW),Spodoptera frugiperda,is a destructive pest native to America and has recently become an invasive insect pest in *** of its rapid spread and great risks in china,understanding of FAW genetic background and pesticide resistance is urgent and essential to develop effective management ***,we assembled a chromosome-level genome of a male FAW(SFynMstLFR)and compared re-sequencing results of the populations from America,Africa,and *** identification of 163 individuals collected from America,Africa and china showed that both C and R strains were found in the American pop-ulations,while only C strain was found in the chinese and African ***,population geno-mics analysis showed that populations from Africa and china have close relationship with significantly genetic differentiation from American *** toge-ther,FAWs invaded into china were most likely origi-nated from *** genomics analysis displayed that the cytochrome p450 gene family is extremely expanded to 425 members in FAW,of which 283 genes are specific to *** of chinese populations with twenty-three pesticides showed the variant patterns of transcriptome profiles,and several detoxification genes such as AOX,UGT and GST spe-cially responded to the *** findings will be useful in developing effective strategies for manage-ment of FAW in china and other invaded areas.

关键词： Spodoptera frugiperda chromosome-level genome population differentiation cytochrome p450 pesticides

松毛虫赤眼蜂一氧化氮合酶基因的克隆、原核表达及在不同滞育阶段的表达谱分析

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昆虫学报 2020年第6期63卷 679-687页

作者：姜雪冰张雪杜文梅邹振张俊杰吉林农业大学生物防治研究所生物防治技术工程研究中心长春130118 中国科学院动物研究所农业虫害鼠害综合治理研究国家重点实验室北京100101

【目的】本研究旨在克隆松毛虫赤眼蜂Trichogramma dendrolimi一氧化氮合酶(NOS)基因,表达其编码蛋白,并探究其在松毛虫赤眼蜂滞育过程中的表达模式,为松毛虫赤眼蜂滞育研究提供新依据。【方法】通过转录组数据分析获得松毛虫赤眼蜂一... 详细信息

【目的】本研究旨在克隆松毛虫赤眼蜂Trichogramma dendrolimi一氧化氮合酶(NOS)基因,表达其编码蛋白,并探究其在松毛虫赤眼蜂滞育过程中的表达模式,为松毛虫赤眼蜂滞育研究提供新依据。【方法】通过转录组数据分析获得松毛虫赤眼蜂一氧化氮合酶基因TdNOS cDNA序列,使用qPCR方法检测其在松毛虫赤眼蜂不同滞育阶段预蛹和蛹中的表达量。使用RT-PCR方法扩增TdNOS cDNA序列并通过生物信息学方法分析其序列特征。构建TdNOS的原核表达载体,利用大肠杆菌Escherichia coli表达系统表达重组TdNOS蛋白,通过Ni-NTA琼脂糖亲和层析纯化,并用于制备多克隆抗体。利用Western blot进一步检测TdNOS在松毛虫赤眼蜂不同滞育阶段的表达量。通过质谱鉴定纯化的目标蛋白。【结果】qPCR分析表明,TdNOS在松毛虫赤眼蜂滞育解除后蛹期的相对表达量显著高于其他阶段的。克隆获得松毛虫赤眼蜂TdNOS cDNA序列(GenBank登录号:MN650600),开放阅读框(ORF)序列长1014 bp,编码337个氨基酸,无信号肽序列,不含跨膜结构,预测有33个丝氨酸磷酸化位点、7个酪氨酸磷酸化位点和10个苏氨酸磷酸化位点。系统发育分析表明,松毛虫赤眼蜂NOS与短管赤眼蜂*** NOS亲缘关系最近,形成与其他膜翅目昆虫NOS蛋白分离的独立分支。成功表达纯化了重组蛋白TdNOS并制备了抗体;Western blot结果表明,TdNOS在松毛虫赤眼蜂滞育解除后蛹期的蛋白表达量高于其他阶段的。质谱检测结果表明纯化的目的蛋白即为TdNOS。【结论】松毛虫赤眼蜂TdNOS在解除滞育后蛹期具有较高的蛋白及mRNA表达量。本研究为进一步探究松毛虫赤眼蜂一氧化氮合酶在滞育发育过程中的作用机制奠定了基础。

关键词：松毛虫赤眼蜂滞育一氧化氮合酶基因克隆原核表达 qPCR

北京市3个公园白纹伊蚊种群Rdl抗性基因突变分析

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中国热带医学 2022年第6期22卷 535-539页

作者：周小洁张洪江赵显丰邱星辉张勇北京市疾病预防控制中心北京100013 北京市朝阳区疾病预防控制中心北京100021 北京市通州区疾病预防控制中心北京101100 中国科学院动物研究所农业虫害鼠害综合治理研究国家重点实验室北京100086

目的对北京市3个大型森林公园白纹伊蚊(Aedes albopictus)野外种群的Rdl基因抗性突变进行检测,评估其对γ-氨基丁酸(γ-aminobutyric acid,GABA)受体类杀虫剂的抗性风险。方法利用序列比对分析获得白纹伊蚊编码GABA受体的Rdl基因序列... 详细信息

目的对北京市3个大型森林公园白纹伊蚊(Aedes albopictus)野外种群的Rdl基因抗性突变进行检测,评估其对γ-氨基丁酸(γ-aminobutyric acid,GABA)受体类杀虫剂的抗性风险。方法利用序列比对分析获得白纹伊蚊编码GABA受体的Rdl基因序列,通过PCR扩增Rdl基因片段并进行测序,分析与抗性有关位点(第A296和V327位点)的突变情况。结果白纹伊蚊Rdl基因由10个外显子(Exon)和9个内含子(Intron)组成,编码区(CDS)全长1 608 bp,编码535个氨基酸;白纹伊蚊Rdl基因编码氨基酸序列与埃及伊蚊(Aedes aegypti)和淡色库蚊(Culex pipiens pallens)相应序列的相似性分别为99.63%,与黑腹果蝇(Drosophila melanogaster)相似性为91.55%;在3个公园采集野外白纹伊蚊种群中(90只标本)均未检测到上述Rdl靶标抗性突变,均为敏感型(GCA和GTA)。结论北京市3个大型的城市森林公园环境中白纹伊蚊种群对环戊二烯类有机氯杀虫剂和苯基吡唑类杀虫剂氟虫腈的靶标(即GABA受体)Rdl基因与其他昆虫表现出不同程度的同源性和高度保守的基因结构特征,3个受试白纹伊蚊野外种群未检测到Rdl基因抗性相关突变,提示以靶标敏感为导向反向研发杀虫剂。

关键词：白纹伊蚊环戊二烯类杀虫剂 Rdl基因抗性突变