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Development of Alfalfa (Medicago sativa L.) Regeneration System and Agrobacterium-Mediated Genetic Transformation

Agricultural Sciences in China 2010年第2期9卷 170-178页

作者： zhang He HUANG qi-man SU Jin Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

The regeneration ability of four alfalfa （Medicago sativa L.） cultivars, Xinjiang Daye, Longdong, Gannong 1 and Gannong 3, was studied, and the effects of various cultivars, explant sources and medium recipes on regeneration were compared. The better callus forming frequency obtained from hypocotyls of Xinjiang Daye is 88.5% and regeneration frequency is 9.8% in our initial experiments. To further optimize regeneration system for genetic transformation, we therefore changed concentrations of plant growth regulators and supplemented with glutamine into callus-induction and shoot-regeneration media. Callus forming frequency and shoot differentiation frequency were increased to 100%. The time taken to generate transgenic plants （16 weeks） was shorter than that for previouse procedure （25 weeks） and regeneration frequency was promoted to 15.1%. The results show that addition of glutamine is particularly important for shortening period of regeneration and promoting regeneration frequency. For study of genetic transformation of alfalfa, Agrobacterium tumefaciens-mediated transformation of Xinjiang Daye was developed based on this optimized regeneration system. The plant expression vector carrying two glutamine synthetases （GS 1 and GS2） and △1-pyrroline-5-carboxylate synthetase （P5CS） gene was used for alfalfa in vitro transformation. Six transgenic alfalfa plantlets with resistance to PPT were obtained. The introduction of foreign genes into plants was assessed in the transformants by PCR analysis and Southern hybridizations.

关键词： alfalf glutamine synthetases △1-pyrroline-5-carboxylate synthetase Agrobacterium tumefaciens-mediated transformation transgenic alfalfa

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Monotropein Induced Apoptosis and Suppressed Cell Cycle Progression in Colorectal Cancer Cells

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Chinese Journal of Integrative Medicine 2024年第1期30卷 25-33页

作者： GAO Quan LI Lin zhang qi-man SHENG qin-song zhang Ji-liang JIN Li-jun SHANG Rui-yan School of Pharmacy and Department of Medical Oncology the Affiliated Hospital of Hangzhou Normal UniversityHangzhou Normal UniversityHangzhou(311121)China Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines Engineering Laboratory of Development and Application of Traditional Chinese MedicinesCollaborative Innovation Center of Traditional Chinese Medicines of Zhejiang ProvinceHangzhou Normal UniversityHangzhou(311121)China Department of Colorectal Surgery the First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouChina Beijing Tong Ren Tang Chinese Medicine Co. Ltd.Beijing(100000)China Department of Traditional Chinese Medicine Hangzhou Shangcheng District People's HospitalHangzhou(310006)China Department of Gynecology Hangzhou Women's HospitalHangzhou(310008)China

Objective: To determine whether monotropein has an anticancer effect and explore its potential mechanisms against colorectal cancer(CRC) through network pharmacology and molecular docking combined with experimental verification. Methods: Network pharmacology and molecular docking were used to predict potential targets of monotropein against CRC. Cell counting kit assay, plate monoclonal assay and microscopic observation were used to investigate the antiproliferative effects of monotropein on CRC cells HCT116, HT29 and LoVo. Flow cytometry and scratch assay were used to analyze apoptosis and cell cycle, as well as cell migration, respectively in HCT116, HT29, and LoVo cells. Western blotting was used to detect the expression of proteins related to apoptosis, cell cycle, and cell migration, and the expression of proteins key to the Akt pathway. Results: The Gene Ontology and Reactome enrichment analyses indicated that the anticancer potential of monotropein against CRC might be involved in multiple cancer-related signaling pathways. Among these pathways, RAC-beta serine/threonine-protein kinase(Akt1, Akt2), cyclin-dependent kinase 6(CDK6), matrix metalloproteinase-9(MMP9), epidermal growth factor receptor(EGFR), cell division control protein 42 homolog(CDC42) were shown as the potential anticancer targets of monotropein against CRC. Molecular docking suggested that monotropein may interact with the 6 targets(Akt1, Akt2, CDK6, MMP9, EGFR, CDC42). Subsequently, cell activity of HCT116, HT29 and LoVo cell lines were significantly suppressed by monotropein(P<0.05). Furthermore, our research revealed that monotropein induced cell apoptosis by inhibiting Bcl-2 and increasing Bax, induced G_1–S cycle arrest in colorectal cancer by decreasing the expressions of CyclinD1, CDK4 and CDK6, inhibited cell migration by suppressing the expressions of CDC42 and MMP9(P<0.05), and might play an anticancer role through Akt signaling pathway. Conclusions: Monotropein exerts its antitumor

关键词： monotropein Chinese medicine anticancer activity colorectal cancer cells network pharmacology

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Summary on Professor qiAO Bao-zhang's Experience in Syndrome Differentiation and Treatment for Pancreatic Cancer

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World Journal of Integrated Traditional and Western Medicine 2020年第11期6卷 31-37页

作者： zhang qi-man ZHENG Jin Shaanxi University of Chinese Medicine Xianyang 712000China Tangdu Hospital of Air Force Medical University Xi'an 710038China

Objective:To summarize clinical experience of Professor qiAO Bao-zhang in treatment for pancreatic cancer,and to provide reference for ***:Through learning from Professor qiAO during his clinical practice and listening to his lectures,Professor qiAO Bao-zhang's clinically proved cases of treating pancreatic cancer based on syndrome differentiation were organized and *** aspects like etiology,pathogenesis,treatment principles and treatment methods of pancreatic cancer,Professor qiAO's experience in diagnosis and treatment for this disease was analyzed and explored,and characteristics of medication were *** typical cases were selected as proof,and his treatment ideas and methods were ***:Professor qiAO believes that the pathogenesis is mostly deficiency of vital qi(正气),and stagnation of phlegm,heat and dampness in Zang-Fu(脏腑)and *** intermingled deficiency and excess,syndrome differentiation and treatment were *** to treatment methods such as strengthening vital qi to eliminate pathogenic factor,clearing heat,eliminating dampness and resolving phlegm,as well as removing toxicity and resolving hard mass,progress of malignant tumors was inhibited and recurrence and metastasis of malignant tumors were ***:Professor qiAO's experience in treating pancreatic cancer is worth learning.

关键词： qiAO Bao-zhang Pancreatic cancer Syndrome differentiation and treatment Medical record Famous doctor's experience

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Asymptotic false discovery control of the Benjamini-Hochberg procedure for pairwise comparisons

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Science China Mathematics 2025年第1期 223-252页

作者： Weidong Liu Dennis Leung qi-man Shao School of Mathematical Sciences Shanghai Jiao Tong University School of Mathematics and Statistics University of Melbourne Department of Statistics and Data Science SICMSouthern University of Science and Technology

In a one-way analysis-of-variance(ANOVA) model,the number of pairwise comparisons can become large even with a moderate number of *** by this,we consider a regime with a growing number of groups and prove that,when testing pairwise comparisons,the Benjamini-Hochberg(BH) procedure can asymptotically control false discoveries,despite the fact that the involved t-statistics do not exhibit the wellknown positive dependence structure required for exact false discovery rate(FDR) *** Tukey's perspective that the difference between the means of any two groups cannot be exactly zero,our main result provides control over the directional false discovery rate and directional false discovery proportion.A key technical contribution of our work is demonstrating that the dependence among the t-statistics is sufficiently weak to establish the convergence result typically required for asymptotic FDR *** analysis does not rely on conventional assumptions such as normality,variance homogeneity,or a balanced design,thereby offering a theoretical foundation for applications in more general settings.

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无线传感器网络中蒙特卡洛定位算法的研究

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计算机科学 2018年第12期45卷 77-80,116页

作者：张绮曼张颖上海海事大学信息工程学院上海201306

在无线传感器网络的节点定位领域,常用的以蒙特卡洛为基础的定位算法均存在定位误差大、采样效率低的问题。为了提高无线传感器网络中针对移动节点的采样效率和定位精确度,文中采用马尔科夫链进行抽样,提出了一种基于蒙特卡洛的改进算... 详细信息

在无线传感器网络的节点定位领域,常用的以蒙特卡洛为基础的定位算法均存在定位误差大、采样效率低的问题。为了提高无线传感器网络中针对移动节点的采样效率和定位精确度,文中采用马尔科夫链进行抽样,提出了一种基于蒙特卡洛的改进算法。该算法在蒙特卡洛算法的基础上,结合马尔科夫链采集节点样本,随后对其进行过滤,再通过对得到的节点位置值进行加权计算,得到节点的准确位置。仿真实验结果表明,通过该算法得到的节点定位误差低于其他算法,提高了采样效率以及对移动节点的定位准确率。

关键词：无线传感器网络蒙特卡洛算法马尔科夫链移动节点定位

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返乡抑或留城:农民工劳动力供求结构分析

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华南农业大学学报（社会科学版） 2023年第3期22卷 57-68页

作者：艾慧张曼琦上海大学经济研究中心上海200433

常住与户籍人口城镇化率的差距拉大及农民工回流表明:农民工市民化政策目标与效果存在差距。上述问题与农民工就业结构、空间配置紧密相关:第一,需求层面:存在农民工就业结构性失衡;城市扩张强化对农民工的劳动力需求,但面临学历错位。... 详细信息

常住与户籍人口城镇化率的差距拉大及农民工回流表明:农民工市民化政策目标与效果存在差距。上述问题与农民工就业结构、空间配置紧密相关:第一,需求层面:存在农民工就业结构性失衡;城市扩张强化对农民工的劳动力需求,但面临学历错位。第二,供给层面:从农民工根本利益出发,对定居意愿、用益物权和住房性质进行Probit计量分析发现:拥有土地承包权和宅基地使用权对定居意愿呈负向影响,土地流转、在城市拥有住房或正规性租房均有正向影响。住房性质(购房/自建房与租房)对用益物权的影响具有调节作用。第三,供求综合分析:就业错位和学历错位影响其城市收入,政策错位可能降低户籍吸引力从而影响迁移意愿,弱化定居意愿的因素占主导。应加强农民工就业技能培训,加强城市尤其是县域的住房保障及公共服务供给,尽快扭转三大错位,推进市民化与助力乡村振兴并进。

关键词：农民工劳动力供求定居意愿结构性错位

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Ammonia pyrolysis oxidation excited by nanosecond pulsed discharge:Global/fluid models hybrid solution

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Journal of Energy Chemistry 2025年第1期100卷 385-396页

作者： Mingming zhang Hanyu Wu qi Chen Jiaxin Yang Bonan Liu School of Mechanical Electronic and Control Engineering Beijing Jiaotong University China University of Petroleum

The kinetic characteristics of plasma-assisted oxidative pyrolysis of ammonia are studied by using the global/fluid models hybrid solution ***,the stable products of plasma-assisted oxidative pyrolysis of ammonia are *** results show that the consumption of NH3/O2and the production of N2/H2change linearly with the increase of voltage,which indicates the decoupling of nonequilibrium molecular excitation and oxidative pyrolysis of ammonia at low ***,the detailed reaction kinetics mechanism of ammonia oxidative pyrolysis stimulated by a nanosecond pulse voltage at low pressure and room temperature is *** on the reaction path analysis,the simplified mechanism is *** detailed and simplified mechanism simulation results are compared with experimental data to verify the accuracy of the simplified ***,based on the simplified mechanism,the fluid model of ammonia oxidative pyrolysis stimulated by the nanosecond pulse plasma is established to study the pre-sheath/sheath behavior and the resultant consumption and formation of key *** results show that the generation,development,and propagation of the pre-sheath have a great influence on the formation and consumption of *** consumption of NH3by the cathode pre-sheath is greater than that by the anode pre-sheath,but the opposite is true for OH and O（1S）.However,within the sheath,almost all reactions do not ***,by changing the parameters of nanosecond pulse power supply voltage,it is found that the electron number density,electron current density,and applied peak voltages are not the direct reasons for the structural changes of the sheath and ***,the discharge interval has little effect on the sheath structure and gas mixture *** research results of this paper not only help to understand the kinetic promotion of non-equilibrium excitation in the process of oxidative pyrolysis but also help to explore th

关键词： Nanosecond pulsed discharge Ammonia Oxidative pyrolysis Fluid model Global model

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体验模型指导下的云办公软件社会化分享设计

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包装工程 2017年第2期38卷 146-150页

作者：张漫琪肖狄虎湖南大学长沙410082

目的探究在体验经济时代下,Saa S模式的云办公软件中的社会化分享设计。方法明确云办公软件的发展趋势及技术应用,用体验模型指导云办公软件的社会化分享设计。结论通过体验模型中五个层面的社会化分享设计,使云办公软件中的社会化分享... 详细信息

目的探究在体验经济时代下,Saa S模式的云办公软件中的社会化分享设计。方法明确云办公软件的发展趋势及技术应用,用体验模型指导云办公软件的社会化分享设计。结论通过体验模型中五个层面的社会化分享设计,使云办公软件中的社会化分享服务更高效、更具人性化,更符合市场发展规律。

关键词：体验模型办公软件社会化分享用户体验设计

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Chemical constituents from the stems of Gymnema sylvestre

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Chinese Journal of Natural Medicines 2014年第4期12卷 300-304页

作者： LIU Yue XU Tun-Hai zhang man-qi LI Xue XU Ya-Juan JIANG Hong-Yu LIU Tong-Hua XU Dong-Ming Jilin Academy of Chinese Medicine Sciences Department of Traditional Chinese Medicine Chemistry School of Traditional Chinese Medicine Beijing University of Chinese Medicine School of Pharmacy China Pharmaceutical University School of Pharmacy Jilin University Department of General Internal Medicine The First Hospital of Jilin University Jilin University

AIM: To study the chemical constituents of stems of Gymnema sylvestre(Retz.) Schult. METHODS: Chromatographic techniques using silica gel, C18 reversed phase silica gel, and prep-HPLC were used. The structures were elucidated on the basis of MS and spectroscopic analysis(1D and 2D NMR), as well as chemical methods. RESULTS: Seven compounds were isolated and their structures were elucidated as conduritol A(1), stigmasterol(2), lupeol(3), stigmasterol-3-O-β-D-glucoside(4), the sodium salt of 22α-hydroxy-longispinogenin-3-O-β-D-glucopyranosyl-(1→3)-β-D-glucurono-pyranosyl-28-O-α-L-rhamnopyranoside(5), oleanolic acid-3-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside(6), and the sodium salt of 22α-hydroxy-longispinogenin 3-O-β-D-glucuronopyranosyl-28-O-α-L-rhamnopyranoside(7). The inhibition activities of compounds 1, 5-7 on non-enzymatic glycation of protein in vitro were evaluated. CONCLUSION: Compound 7 is a new triterpenoid saponin. It was shown that compounds 1, 5-7 have weak inhibition activities for non-enzymatic glycation of protein in vitro.

关键词： Gymnema sylvestre Triterpenoid saponin Non-enzymatic glycation-inhibiting activity

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Lipid droplets in the nervous system:involvement in cell metabolic homeostasis

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Neural Regeneration Research 2025年第3期20卷 740-750页

作者： Yuchen zhang Yiqing Chen Cheng Zhuang Jingxuan qi Robert Chunhua Zhao Jiao Wang School of Life Sciences Shanghai UniversityShanghaiChina School of Medicine Shanghai UniversityShanghaiChina Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic MedicinePeking Union Medical CollegeBeijingChina Center of Excellence in Tissue Engineering Chinese Academy of Medical SciencesBeijingChina Beijing Key Laboratory of New Drug Development and Clinical Trial of Stem Cell Therapy BeijingChina

Lipid droplets serve as primary storage organelles for neutral lipids in neurons,glial cells,and other cells in the nervous *** droplet formation begins with the synthesis of neutral lipids in the endoplasmic ***,lipid droplets were recognized for their role in maintaining lipid metabolism and energy homeostasis;however,recent research has shown that lipid droplets are highly adaptive organelles with diverse functions in the nervous *** addition to their role in regulating cell metabolism,lipid droplets play a protective role in various cellular stress ***,lipid droplets exhibit specific functions in neurons and glial *** of lipid droplet formation leads to cellular dysfunction,metabolic abnormalities,and nervous system *** review aims to provide an overview of the role of lipid droplets in the nervous system,covering topics such as biogenesis,cellular specificity,and ***,it will explore the association between lipid droplets and neurodegenerative *** the involvement of lipid droplets in cell metabolic homeostasis related to the nervous system is crucial to determine the underlying causes and in exploring potential therapeutic approaches for these diseases.

关键词： Alzheimer's disease lipid droplet biogenesis lipid droplets lipid metabolism nervous system neurodegenerative disorders oxidative stress Parkinson's disease

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