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补阳还五汤“异病同治”2型糖尿病和阿尔兹海默症作用机制的网络药理学分析

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中药新药与临床药理 2024年第9期35卷 1364-1375页

作者：薛慧徐艳明姜晶孟雪彤刘书萌周倩雷霞张宁黑龙江中医药大学药学院黑龙江哈尔滨150040 黑龙江中医药大学佳木斯学院黑龙江佳木斯154007 南京中医药大学无锡附属医院/江苏省中医退行性骨关节病临床医学创新中心江苏无锡214071

目的基于网络药理学和分子对接技术探讨补阳还五汤对2型糖尿病(T2DM)和阿尔兹海默症(AD)“异病同治”的作用机制。方法借助TCMSP、SymMap等数据库检索和筛选补阳还五汤中7味组方中药的活性成分,并利用PharmMapper服务器对活性成分进行... 详细信息

目的基于网络药理学和分子对接技术探讨补阳还五汤对2型糖尿病(T2DM)和阿尔兹海默症(AD)“异病同治”的作用机制。方法借助TCMSP、SymMap等数据库检索和筛选补阳还五汤中7味组方中药的活性成分,并利用PharmMapper服务器对活性成分进行靶点预测。通过OMIM、DrugBank、GeneCards及Disgenet数据库收集T2DM和AD疾病相关靶点。对疾病相关靶点与活性成分作用靶点取交集,获得补阳还五汤“异病同治”T2DM和AD的潜在作用靶点。使用STRING数据库和Cytoscape软件分别构建潜在作用靶点的蛋白-蛋白互作(PPI)网络和“药物-成分-靶点”网络,通过网络拓扑分析筛选出核心活性成分和核心靶点。并借助Metascape数据库对潜在作用靶点进行GO功能及KEGG通路富集分析。采用AutoDock软件对筛选出的核心活性成分和核心靶点进行分子对接验证。结果94个补阳还五汤活性成分可作用于342个蛋白靶点,与3140个AD疾病相关靶点和1708个T2DM疾病相关靶点比对后,得到100个潜在作用靶点(交集靶点)。GO功能富集分析显示,潜在作用靶点主要参与MAPK级联调控、激素介导的信号通路、细胞对脂质的反应、调节炎症反应等生物过程。KEGG通路富集分析得到MAPK、PI3K/Akt、FoxO、AGE/RAGE、胰岛素抵抗、脂质和动脉粥样硬化、非酒精性脂肪肝等通路。PPI和“药物-成分-靶点”网络拓扑分析筛选出MAPK8、MAPK14、GSK3B、PPARG等10个核心靶点,以及芍药苷、苯甲酰芍药苷、(+)-儿茶素等10个核心活性成分,分子对接结果表明上述成分与靶点均有较强的结合能力。结论补阳还五汤可能通过芍药苷、儿茶素等核心成分,作用于PPARG、GSK3B等关键靶点,参与调控MAPK、PI3K/Akt等信号通路,进而发挥“异病同治”T2DM和AD的作用。

关键词：补阳还五汤 2型糖尿病阿尔兹海默症异病同治网络药理学分子对接

High-dose thalidomide increases the risk of peripheral neuropathy in the treatment of ankylosing spondylitis

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Neural Regeneration Research 2015年第5期10卷 814-818页

作者： hong-xia xue Wen-yi Fu hua-dong cui Li-li Yang ning zhang Li-juan Zhao Department of Rheumatology and Immunology Shengjing Hospital of China Medical University Department of Rheumatology and Immunology the First Affiliated Hospital of China Medical University

Thalidomide is an effective drug for the treatment of ankylosing spondylitis but might induce peripheral neuropathy. This major adverse reaction has attracted much concern. The current study aimed to observe the incidence of thalidomide-induced peripheral neuropathy among an- kylosing spondylitis patients for 1 year after treatment. In this study, 207 ankylosing spondylitis cases received thalidomide treatment, while 116 ankylosing spondylitis cases received other treat- ments. Results showed that the incidence of thalidomide-induced peripheral neuropathy in the thalidomide group was higher than that in the non-thalidomide group. There was no significant difference in the incidence of neuropathy between the 〈 6 months medication and 〉 6 months medication groups. There were no differences in the mean age, gender, or daily dose between the two groups. The incidence of peripheral neuropathy among patients receiving 25, 50, 75, or 100 mg thalidomide per day was 4.6%, 8.5%, 17.1%, 21.7%, respectively. The incidence was significantly different between the groups receiving 25 mg and 100 mg thalidomide. In conclu- sion, thalidomide can induce peripheral neuropathy within 1 year after treatment of ankylosing spondylitis; however, age and gender have no obvious impact on the incidence of peripheral neuropathy. The incidence of peripheral neuropathy is associated with increasing daily doses of thalidomide.

关键词： nerve regeneration peripheral nerve injury thalidomide ankylosing spondflitis adversereactions peripheral neuropathy prospective study treatment dose treatment time age sex neuralregeneration

超高效液相色谱-三重四极杆质谱法同时测定消肿膏中6种乌头型生物碱含量

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中国临床药理学杂志 2024年第9期40卷 1341-1344页

作者：夏天利赵德萍薛傲金美玲朱紫悦李卿张宁雷霞佳木斯大学药学院黑龙江佳木斯154007 黑龙江中医药大学中医药研究所黑龙江哈尔滨150040 无锡市中医医院江苏省中医退行性骨关节病临床医学创新中心江苏无锡214071

目的用超高效液相色谱-三重四极杆质谱串联(UPLC-TQD-MS)法同时测定消肿膏中乌头碱、新乌头碱、次乌头碱、苯甲酰乌头原碱、苯甲酰新乌头原碱、苯甲酰次乌头原碱的含量。方法用ACQUITY UPLC BEH C18柱(50 mm×2.1 mm,1.7μm),流动相为0... 详细信息

目的用超高效液相色谱-三重四极杆质谱串联(UPLC-TQD-MS)法同时测定消肿膏中乌头碱、新乌头碱、次乌头碱、苯甲酰乌头原碱、苯甲酰新乌头原碱、苯甲酰次乌头原碱的含量。方法用ACQUITY UPLC BEH C18柱(50 mm×2.1 mm,1.7μm),流动相为0.1%甲酸水(A)-乙腈(B),梯度洗脱,柱温为40℃,流速为0.3 mL·min^(-1),进样量5μL;用电喷雾离子源正离子模式(ESI+)和多反应监测模式(MRM)进行质谱分析。结果乌头碱、新乌头碱、次乌头碱、苯甲酰乌头原碱、苯甲酰新乌头原碱、苯甲酰次乌头原碱的质量浓度均在1.0~100.0 ng·mL^(-1)呈良好线性关系,平均加样回收率为98.62%~101.24%。3个批样品中,6个成分的质量分数分别为0.18、0.33、0.38、0.43、0.28、0.06μg·g^(-1)。结论该方法可用于检测消肿膏中6种乌头型生物碱含量,为消肿膏的质量评价及临床安全用药提供参考。

关键词：消肿膏乌头型生物碱超高效液相色谱-三重四极杆质谱法含量测定

Preparation of chitosan/N-doped graphene natively grown on hierarchical porous carbon nanocomposite as a sensor platform for determination of tartrazine

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Chinese Chemical Letters 2017年第7期28卷 1492-1498页

作者： Zhen-Zhen An Zhuang Li Yong-Yang Guo xiao-Ling Chen Kang-ning zhang dong-xia zhang Zhong-hua xue Xi-Bin Zhou xiao-Quan Lu Key Laboratory of Bioelectrochemistry&Environmental Analysis of Gansu Province College of Geography and Environment ScienceNorthwest Normal UniversityLanzhou 730070China College of Chemistry and Chemical Engineering Northwest Normal UniversityLanzhou 730070China

In this work,the chitosan and N-doped graphene natively grown on hierarchical porous carbon（N-PC-G/CS） nanocomposite was obtained by ultrasonic method,as a novel sensor platform for determination of tartrazine（TT）.The nanocomposite as prepared had well dispersivity in water and excellent *** N-PC-G/CS nanocomposite was characterized by scanning electron microscopy（SEM）,transmission electron microscopy（TEM）,nitrogen adsorption-desorption,fourier transform infrared（FTIR） and electrochemical impedance spectroscopy（EIS）.The application of N-PC-G/CS for determination of tartrazine（TT） was investigated by chronocoulometry（CC）,cyclic voltammetry（CV） and differential pulse voltammetry（DPV）.Under optimized conditions,the sensor displayed a sensitive response to TT within a wide concentration range of 0.05-15.0 μmol/L,the detection limits is 0.036 μmol/L（S/N = 3）.Furthermore,this nanocomposite could be efficiently applied for determination of TT in soft drink samples.

关键词： Porous carbon Graphene Chitosan Sensor Tartrazine

两种Rh血型系统抗体导致交叉配血不合原因分析与实验室处理策略

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细胞与分子免疫学杂志 2024年第7期40卷 623-628页

作者：崔颖张瑜杨世明许亚莉齐霞邢艳安宁王宝燕西安交通大学第一附属医院输血科陕西西安710061 唐都医院输血科陕西西安710038 空军军医大学第一附属医院输血科陕西西安710032

目的分析RhDC、RhEc抗原阴性患者血清中存在IgG型抗DC及抗Ec联合抗体的血型血清学特点及临床意义。方法回顾性分析12例血清中存在两种Rh系统不规则抗体的病例的临床资料及实验室检查结果,包括年龄、性别、输血史/妊娠史及血型血清学检... 详细信息

目的分析RhDC、RhEc抗原阴性患者血清中存在IgG型抗DC及抗Ec联合抗体的血型血清学特点及临床意义。方法回顾性分析12例血清中存在两种Rh系统不规则抗体的病例的临床资料及实验室检查结果,包括年龄、性别、输血史/妊娠史及血型血清学检测结果(ABO、RhD血型鉴定及Rh分型结果、不规则抗体筛选、抗体特异性鉴定、吸收放散试验、抗体效价测定、交叉配血试验)。结果12例患者中,年龄(51.4±16.9)岁。9例有输血史,8例有妊娠史,5例既有输血史又有妊娠史。在血型血清学检测中抗体筛选阳性、交叉配血不合。抗体特异性鉴定及吸收放散试验结果显示,3例患者血清中同时存在IgG型抗D、抗C抗体,抗D效价16~32、抗C效价8~16;9例患者血清中同时存在IgG型抗E、抗c抗体,抗E、抗c效价均为8~16;抗D、抗C联合抗体的患者选择ABO同型RhD阴性,其他Rh抗原为ccee的悬浮红细胞,抗E、抗c联合抗体的患者选择ABO同型RhD阳性,其他Rh抗原为CCee的悬浮红细胞,交叉配血试验结果显示,在盐水、聚凝胺、抗人球蛋白介质中均无凝集、无溶血。结论输血和(或)妊娠免疫是产生两种Rh系统不规则抗体、导致抗体筛选阳性及交叉配血不合的主要原因,ABO同型基础上常规进行Rh配合性输注,对于临床输血的安全性和预防新生儿溶血病的发生具有重要的价值和意义。

关键词： Rh血型交叉配血不合 Rh同型输注

基于面部表情分析技术的葡萄酒中关键香气与饮用舒适度相关性评价

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食品与发酵工业 2024年第8期50卷 146-153,I0002-I0005页

作者：黄翠武运薛洁皇甫洁孙志伟唐家乐张成学李宁宋涛新疆农业大学食品科学与药学学院新疆乌鲁木齐830052 中国食品发酵工业研究院北京100015 国家酒类品质与安全国际联合研究中心北京100015

为明晰新疆地产葡萄酒中影响饮用舒适度的关键香气物质,以新疆四大产区葡萄酒为试材,采用顶空固相微萃取(headspace solid-phase microextraction,HS-SPME)与GC-MS联用技术,结合香气活性值(odor activity value,OAV)确定11款葡萄酒中的... 详细信息

为明晰新疆地产葡萄酒中影响饮用舒适度的关键香气物质,以新疆四大产区葡萄酒为试材,采用顶空固相微萃取(headspace solid-phase microextraction,HS-SPME)与GC-MS联用技术,结合香气活性值(odor activity value,OAV)确定11款葡萄酒中的关键香气,基于模糊数学传统感官与智能感官面部表情分析对11款酒的感官质量进行综合评价并建立线性回归模型,将关键情绪维度与关键香气物质作相关性分析。研究结果表明,共定性定量了79种香气物质(共有组分43种),酯类数量最多(35种),醇类次之(19种),萜烯类(11种)、醛酮类(7种)、酸类(4种)和其他类(3种)最少。其中吐哈盆地赤霞珠(2号)、焉耆盆地马瑟兰(7号)、焉耆盆地霞多丽(8号)品评得分结果较高;线性回归模型R^(2)=0.831且拟合度良好,说明面部表情分析中其效价及唤醒度能基本反映综合饮用舒适度。通过相关性分析确定与饮用舒适度相关的关键物质为乙酸乙酯、异戊醇、活性戊醇、甲醇、3-羟基-2-丁酮、辛酸等。该研究为提高新疆葡萄酒饮用舒适度奠定了科学理论依据。

关键词：葡萄酒香气面部表情综合评价饮用舒适度

Effects of nitrogen form on growth,CO_2 assimilation,chlorophyll fluorescence,and photosynthetic electron allocation in cucumber and rice plants

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第2期12卷 126-134页

作者： Yan-hong ZHOU Yi-li zhang xue-min WANG Jin-xia cui xiao-jian xia Kai SHI Jing-quan YU Department of Horticulture Zhejiang University Hangzhou 310029 China Key Laboratory of Horticultural Plants Growth Development and Quality Improvement Ministry of Agriculture Hangzhou 310029 China

Cucumber and rice plants with varying ammonium (NH4+) sensitivities were used to examine the effects of different nitrogen (N) sources on gas exchange, chlorophyll (Chl) fluorescence quenching, and photosynthetic electron allocation. Compared to nitrate (NO3-)-grown plants, cucumber plants grown under NH4+-nutrition showed decreased plant growth, net photosynthetic rate, stomatal conductance, intercellular carbon dioxide (CO2) level, transpiration rate, maximum photochemical efficiency of photosystem II, and O2-independent alternative electron flux, and increased O2-dependent alternative electron flux. However, the N source had little effect on gas exchange, Chl a fluorescence parameters, and photosynthetic electron allocation in rice plants, except that NH4+-grown plants had a higher O2-independent alternative electron flux than NO3--grown plants. NO3- reduction activity was rarely detected in leaves of NH4+-grown cucumber plants, but was high in NH4+-grown rice plants. These results demonstrate that significant amounts of photosynthetic electron transport were coupled to NO3- assimilation, an effect more significant in NO3-- grown plants than in NH4+-grown plants. Meanwhile, NH4+-tolerant plants exhibited a higher demand for the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) for NO3- reduction, regardless of the N form supplied, while NH4+-sensitive plants had a high water-water cycle activity when NH4+ was supplied as the sole N source.

关键词： Nitrogen form Photosynthetic electron allocation Alternative electron flux Nitrate reductase

Lattice damage in InGaN induced by swift heavy ion irradiation

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Chinese Physics B 2022年第10期31卷 467-472页

作者： ning Liu Li-Min zhang xue-Ting Liu Shuo zhang Tie-Shan Wang hong-xia Guo State Key Laboratory of Laser Interaction with Matter Northwest Institute of Nuclear TechnologyXi’an 710024China School of Nuclear Science and Technology Lanzhou UniversityLanzhou 730000China

The microstructural responses of In_(0.32)Ga_(0.68)N and In_(0.9)Ga_(0.1)N films to 2.25 GeV Xe ion irradiation have been investigated using x-ray diffraction,Raman scattering,ion channeling and transmission electron *** was found that the In-rich In_(0.9)Ga_(0.1)N is more susceptible to irradiation than the Ga-rich In_(0.32)Ga_(0.68)*** ion irradiation with a fluence of 7×10^(11)ions·cm^(-2)leads to little damage in In_(0.32)Ga_(0.68)N but an obvious lattice expansion in In_(0.9)Ga_(0.1)*** level of lattice disorder in In_(0.9)Ga_(0.1)N increases after irradiation,due to the huge electronic energy deposition of the incident Xe ***,no Xe ion tracks were observed to be formed,which is attributed to the very high velocity of 2.25 Ge V Xe *** defects and/or small defect clusters are probably the dominant defect type in Xe-irradiated In_(0.9)Ga_(0.1)N.

关键词： In GaN swift heavy ions irradiation effects

Properties of polydimethylsiloxane hydrophobic modified duplex microarc oxidation/diamond-like carbon coatings on AZ31B Mg alloy

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Journal of Magnesium and Alloys 2021年第4期9卷 1291-1303页

作者： xue-Jun cui Chuang-Ming ning Guang-An zhang Lun-Lin Shang Li-Ping Zhong Ying-Jun zhang School of Materials Science and Engineering Sichuan University of Science and EngineeringZigong 643000China State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China Key Laboratories of Fine Chemicals and Surfactants in Sichuan Provincial Universities Zigong 643000China

A reliable,high-performance coating procedure was developed using PDMS to modify a duplex MAO/DLC coating on an AZ31B Mg ***,the duplex MAO/DLC coating was fabricated via a combined MAO and unbalanced magnetron sputter ***,a PDMS solution was used to modify the MAO/DLC coating via a conventional dip-coating *** surface characteristics,bond strength,hardness,tribological behaviour,and corrosion resistance of the coated samples were evaluated via SEM,CA,Raman spectroscopy,friction and wear behaviour,polarisation curve,and NSS *** PDMS modification reduced the HIT of MAO/DLC coating from 15.96 to 8.34GPa;this is ascribed to the penetration of PDMS,which has good rheological properties to form a viscoelastic Si-based organic polymer layer on the MAO/DLC ***,the PDMS-modified MAO/DLC coating was denser,hydrophobic,and had higher bond strength compared with MAO-and MAO/DLC-coated ***,the PDMS modification reduced the COF and wear rate of the duplex MAO/DLC *** indicates that the PDMS improved the tribological behaviour owing to the transferred Si oxide that originated from the Si-O network of the PDMS,as well as the low graphitisation of the DLC layer during ***,the corrosion current density of the MAO/DLC-coated sample modified by PDMS for 10min decreased by two order of magnitude compared with that of the MAO/DLC-coated sample but by five orders of magnitude compared with that of the bare *** NSS tests proved that the PDMS layer slowed the corrosion of the Mg alloy under long-term service,enhancing the corrosion protection *** results are attributed to the high bond strength and lubricant MAO/DLC layer,and the dual role of sealing and hydrophobicity of ***,PDMS modification is promising for the fabrication of protective materials for Mg alloys that require corrosion and wear resistance.

关键词： Magnesium alloy Microarc oxidation Diamond-like carbon Polydimethylsiloxane Tribological behaviour Corrosion resistance