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Generation of seed lipoxygenase-free soybean using CRISPR-Cas9

The Crop Journal 2020年第3期8卷 432-439页

作者： Jie Wang Huaqin Kuang Zhihui zhang Yongqing Yang Long Yan mengchen zhang Shikui Song Yuefeng Guan College of Resources and Environment Fujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina FAFU-UCR Joint Center for Horticultural Plant Biology and Metabolomics Haixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Root Biology Center College of Resources and EnvironmentFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina The Key Laboratory of Crop Genetics and Breeding of Hebei Institute of Cereal and Oil CropsHebei Academy of Agricultural and Forestry SciencesShijiazhuang 050035HebeiChina

Beany flavor induced by three lipoxygenases(LOXs, including LOX1, LOX2, and LOX3)restricts human consumption of soybean. It is desirable to generate lipoxygenase-free new mutant lines to improve the eating quality of soybean oil and protein products. In this study, a pooled clustered regularly interspaced short palindromic repeats(CRISPR)-CRISPRassociated protein 9(Cas9) strategy targeting three GmLox genes(GmLox1, GmLox2, and GmLox3) was applied and 60 T_0 positive transgenic plants were generated, carrying combinations of sg RNAs and mutations. Among them, GmLox-28 and GmLox-60 were gmlox1 gmlox2 gmlox3 triple mutants and GmLox-40 was a gmlox1 gmlox2 double *** of T_1 mutant plants derived from GmLox-28, GmLox-60, and GmLox-40 showed that mutation in the GmLox gene was inherited by the next generation. Colorimetric assay revealed that plants carrying different combinations of mutations lost the corresponding lipoxygenase activities. Transgene-free mutants were obtained by screening the T_2 generation of lipoxygenase-free mutant lines(GmLox-28 and GmLox-60). These transgeneand lipoxygenase-free mutants could be used for soybean beany flavor reduction without restriction by regulatory frameworks governing transgenic organisms.

关键词： LOX Generation of seed lipoxygenase-free soybean using CRISPR-Cas9 CRISPR

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Oil-soluble polymer brushes-functionalized nanoMOFs for highly efficient friction and wear reduction

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Friction 2024年第7期12卷 1499-1511页

作者： Jianxi LIU Yong QIAN Dongsheng LI Wei WU mengchen zhang Jie YAN Bin LI Feng ZHOU State Key Laboratory of Solidification Processing Center of Advanced Lubrication and Seal MaterialsSchool of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi'an 710072China Shaanxi Engineering Research Center of Special Sealing Technology Xi'an Aerospace Propulsion InstituteXi'an 710100China State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China

Nanomaterials as lubricating oil additives have attracted significant attention because of their designable composition and structure,suitable mechanical property,and tunable surface ***,the poor compatibility between nanomaterials and base oil limits their further *** this work,we demonstrated oil-soluble poly(lauryl methacrylate)(PLMA)brushes-grafted metal-organic frameworks nanoparticles(nanoMOFs)as lubricating oil additives that can achieve efficient friction reduction and anti-wear *** were synthesized by free-radical polymerization,which was coordinatively grafted onto the surface of the UiO-67 ***,PLMA brushes were grown on the macroinitiator-modified UiO-67 by surface-initiated atom transfer radical polymerization,which greatly improved the lipophilic property of the UiO-67 nanoparticles and significantly enhanced the colloidal stability and long-term dispersity in both non-polar solvent and base *** adding UiO-67@PLMA nanoparticles into the 500 SN base oil,coefficient of friction and wear volume reductions of 45.3%and 75.5%were achieved due to their excellent mechanical properties and oil ***,the load-carrying capacity of 500 SN was greatly increased from 100 to 500 N by the UiO-67@PLMA additives,and their excellent tribological performance was demonstrated even at a high friction frequency of 65 Hz and high temperature of 120℃.Our work highlights oil-soluble polymer brushes-functionalized nanoMOFs for highly efficient lubricating additives.

关键词： MOFs polymer brushes surface modification tribological properties friction reduction anti-wear

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Classification and Identification of indica P/TGMS Lines in China

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Rice science 2019年第3期26卷 195-198页

作者： zhang mengchen WANG Shan YAN Jianfang SUN Shuiyong XU Xin XU Qun YUAN Xiaoping WEI Xinghua YANG Yaolong State Key Laboratory of Rice Biology China National Rice Research Institute Seeds Administration Center of Zhejiang Province

Despite extensive studies on cultivated rice, the population structure and genetic diversity of the indica photoperiod-and thermo-sensitive genic male sterility(P/TGMS) lines in China remains unclear. Using 48 simple sequence repeat(SSR) markers, we genotyped a panel of 208 indica P/TGMS lines and confirmed three subgroups, named indica-I, indica-II and indica-III, in indica P/TGMS lines. Further diversity analysis indicated indica-II had the highest genetic diversity. The genetic differentiation between indica-II and indica-III was demonstrated as the largest among the three subgroups. Moreover, indica/japonica component identification was detected that five P/TGMS lines possess indica components less than 0.900. These results improve our knowledge on the genetic background for P/TGMS lines in China and will be beneficial for hybrid rice breeding programs.

关键词： Classification Identification Despite

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Molecular Evolution of Rice Blast Resistance Gene bsr-d1

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Rice science 2024年第6期31卷 700-711页

作者： LI Wei zhang mengchen YANG Yaolong WENG Lin HU Peisong WEI Xinghua Research Institute of Life Sciences Computing Zhejiang LabHangzhou 310012China State key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024China

Rice blast,caused by the fungus Magnaporthe oryzae,reduces rice yields by 10%to 35%.Incorporating blast resistance genes into breeding programs is an effective strategy to combat this *** the genetic variants that confer resistance is crucial to this *** gene Bsr-d1 encodes a C2H2-like transcription factor,and its recessive allele confers broad-spectrum resistance against infections by various strains of *** this study,we investigated the molecular evolution of the rice blast resistance gene bsr-d1 in a representative population consisting of 827 cultivated and wild rice *** results revealed that wild rice exhibited significantly higher nucleotide diversity,with polymorphic regions primarily concentrated in the promoter region,in contrast to indica and japonica rice *** Bsr-d1 gene displayed significant differentiation between indica and japonica rice varieties,with the bsr-d1 resistance allele being unique to indica *** network and phylogenetic analyses suggested that the bsr-d1 resistance allele most likely originated from Oryza nivara in the region adjacent to the Indian Peninsula and the Indochina ***,we explored the utilization of bsr-d1 resistance alleles in China and designed a pair of DNA primers based on the polymorphic sites for the detection of the bsr-d1 resistance *** summary,our study uncovering the origin and evolution of bsr-d1 will enhance our understanding of resistance gene variation and expedite the resistance breeding process.

关键词： broad-spectrum resistance bsr-d1 gene evolution Magnaporthe oryzae resistance breeding

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Identification of the BTA8 gene reveals the contribution of natural variation to tiller angle in rice

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Journal of Integrative Agriculture 2024年第8期23卷 2868-2871页

作者： Junrong Liu Xingyu Wang Jing Wang Junhua Ye mengchen zhang Qun Xu Yaolong Yang Xinghua Wei Baoyan Jia Yue Feng State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China College of Agronomy Shenyang Agricultural UniversityShenyang 110866China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024China

Plant architecture is a collection of major agronomic traits that determines rice grain production,and it is mainly influenced by tillering,tiller angle,plant height and panicle morphology(Wang and Li 2006).Tiller angle is one of the critical components that determines rice plant architecture,which in turn influences grain yield mainly due to its large impact on plant density(Wang et al.2022).

关键词： angle panicle agronomic

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Genetic contribution of foreign germplasm to elite Chinese soybean (Glycine max) cultivars revealed by SSR markers

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Chinese Science Bulletin 2006年第9期51卷 1078-1084页

作者： QIN Jun CHEN Weiyuan GUAN Rongxia JIANG Chengxi LI Yinghui FU Yashu LIU zhangxiong zhang mengchen CHANG Ruzhen QIU Lijuan National Key Facility of Crop Gene Resources and Genetic Improvement Key Lab of Crop Gerrnplasm Resources & Biotechology (MOA) Institute of Crop Science Chinese Agricultural Academy of Sciences Beijing 100081 China Hebei Academy of Agricultural and Forestry Science Shijiazhuang 050031 China Heilongjiang Academy of Agricultural Science Suihua 152052 China

Simple sequence repeats (SSR) marker analysis, combined with pedigree analysis, was car- ried out to trace the genetic constitutes of Suinong 14 and Hefeng 25 in assessing the contribution of for- eign germplasm to those elite cultivars. The overall goal is to accumulate information for further effective utilization of foreign germplasm in Chinese soybean breeding programs. SSR clustering results indicated that the genetic base of elite Chinese soybean culti- vars, including Suinong 14 and Hefeng 25, were broadened and enhanced via introduction of Amsoy from the U.S. and Shishengchangye from Japan. The pedigree analysis showed a very high coefficient of parentage between Shishengchangye and Hefeng 25, between Shishengchangye and Suinong 14, and between Amsoy and Suinong 14. The genetic simi- larity between Suinong 14 and Hefeng 25 was 60.58%. Among 20 linkage groups, more genomic regions were transferred from Hefeng 25 to Suinong 14 in linkage group (LG) I, L and C2 than other LGs. Among the unique alleles of foreign parents, five specific loci in Amsoy were transferred to Suinong 14, and three loci from Shishengchangye to Suinong 14. Some SSR loci were proved to be correlated with phenotypes: two loci for seed size introgressed from Shishengchangye to Suinong 14, and one locus forprotein content from Amsoy to Suinong 14. These results indicate that the two foreign parents might have important contributions in the development of Suinong 14 and Hefeng 25.

关键词：大豆谱系分析 SSR 序列分析种质资源

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MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean

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Science China(Life Sciences) 2021年第9期64卷 1533-1545页

作者： Xiaolong Fang Xiaoyuan Sun Xiangdong Yang Qing Li Chunjing Lin Jie Xu Wenjun Gong Yifan Wang Lu Liu Limei Zhao Baohui Liu Jun Qin mengchen zhang Chunbao zhang Fanjiang Kong Meina Li Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006China Soybean Research Institute National Engineering Research Center for SoybeanJilin Academy of Agricultural SciencesChangchun 130033China State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou 311401China Core Facility and Technical Service Center for SLSB School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Cereal&Oil Crop Institute Hebei Academy of Agricultural and Forestry SciencesShijiazhuang 050031China

Male sterility is an essential trait in hybrid seed production,especially for monoclinous and autogamous food *** male-sterile ms1 mutant has been known for more than 50 years and could be instrumental in making hybrid ***,the gene responsible for the male-sterile phenotype has remained ***,we report the map-based cloning and characterization of the MS1 gene in ***1 encodes a kinesin protein and localizes to the nucleus,where it is required for the male meiotic cytokinesis after telophase Ⅱ.We further substantiated that MS1 colocalizes with microtubules and is essential for cell plate formation in soybean male gametogenesis through *** ms1 and CRISPR/Cas9 knockout mutants show complete male sterility but are otherwise phenotypically normal,making them perfect tools for producing hybrid *** identification of MS1 has the practical potential for assembling the sterility system and speeding up hybrid soybean breeding.

关键词： soybean male sterility ms1 kinesin cytokinesis hybrid breeding

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Brittle culm 25, which encodes an UDP-xylose synthase, affects cell wall properties in rice

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The Crop Journal 2023年第3期11卷 733-743页

作者： Siliang Xu mengchen zhang Junhua Ye Dongxiu Hu Yuanyuan zhang Zhen Li Junrong Liu Yanfei Sun Shan Wang Xiaoping Yuan Yue Feng Qun Xu Xinghua Wei Dali Zeng Yaolong Yang China National Center for Rice Improvement/State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006ZhejiangChina The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province Zhejiang A&F UniversityHangzhou 311300ZhejiangChina

Because plant mechanical strength influences plant growth and development,the regulatory mechanisms underlying cell-wall synthesis deserve *** mutants are useful for such *** have identified a novel brittle culm 25(bc25)mutant with reduced growth and partial ***25 encodes an UDP-glucuronic acid decarboxylase involved in cellulose synthesis and belongs to the UXS family.A single-nucleotide mutation in BC25 accounts for its altered cell morphology and cellwall *** electron microscopy analysis showed that the thickness of the secondary cell wall was reduced in *** analysis revealed significant increases in content of rhamnose and arabinose but not of other monosaccharides,indicating that BC25 was involved in xylose synthesis with some level of functional *** assays suggested that BC25 functions with high activity to interconvert UDP-glucuronic acid(UDP-Glc A)and *** staining showed that BC25 was ubiquitously expressed with higher expression in culm,root and sheath,in agreement with that shown by quantitative real-time(q RT)-***-seq further suggested that BC25 is involved in sugar *** conclude that BC25 strongly influences rice cell wall formation.

关键词： Brittle culm Mechanical strength UDP-xylose Cell wall Rice

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UNSTEADY NATURAL CONVECTIVE BOUNDARY LAYER FLOW AND HEAT TRANSFER OF FRACTIONAL SECOND-GRADE NANOFLUIDS WITH DIFFERENT PARTICLE SHAPES

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Annals of Applied Mathematics 2020年第3期36卷 257-269页

作者： Ming Shen Yuhang Wu mengchen zhang College of Mathematics and Computer Science Fuzhou UniversityFuzhou 350108FujianPR China

The present study is concerned with unsteady natural convective boundary layer flow and heat transfer of fractional second-grade nanofuids for different particle *** boundary layer governing equations are formulated with time fractional derivatives in the momentum *** governing boundary layer equations of continuity,momentum and energy are reduced by dimensionless *** solutions of the momentum and energy equations are obtained by the finite difference method combined with *** quantites of physical interest are graphically presented and discussed in *** is found that particle shape,fractional derivative parameter and the Grashof number have profound influences on the the flow and heat transfer.

关键词： second-grade nanofluid heat transfer Caputo derivative particle shape

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BTA2 regulates tiller angle and the shoot gravity response through controlling auxin content and distribution in rice

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Journal of Integrative Plant Biology 2024年第9期66卷 1966-1982页

作者： Zhen Li Junhua Ye Qiaoling Yuan mengchen zhang Xingyu Wang Jing Wang Tianyi Wang Hongge Qian Xinghua Wei Yaolong Yang Lianguang Shang Yue Feng China National Center for Rice Improvement State Key Laboratory of Rice Biology and BreedingChina National Rice Research InstituteHangzhou 310006China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518124China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024China

Tiller angle is a key agricultural trait that establishes plant architecture,which in turn strongly affects grain yield by influencing planting density in *** shoot gravity response plays a crucial role in the regulation of tiller angle in rice,but the underlying molecular mechanism is largely ***,we report the identification of the BIG TILLER ANGLE2(BTA2),which regulates tiller angle by controlling the shoot gravity response in ***-of-function mutation of BTA2 dramatically reduced auxin content and affected auxin distribution in rice shoot base,leading to impaired gravitropism and therefore a big tiller ***2 interacted with AUXIN RESPONSE FACTOR7(ARF7)to modulate rice tiller angle through the gravity signaling *** BTA2 protein was highly conserved during *** variation in the BTA2 promoter of indica cultivars harboring a less expressed BTA2 allele caused lower BTA2 expression in shoot base and thus wide tiller angle during rice *** of BTA2 significantly increased grain yield in the elite rice cultivar Huanghuazhan under appropriate dense planting *** findings thus uncovered the BTA2-ARF7 module that regulates tiller angle by mediating the shoot gravity *** work offers a target for genetic manipulation of plant architecture and valuable information for crop improvement by producing the ideal plant type.

关键词： auxin domestication gravity response rice tiller angle

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