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Transcriptome-Wide Identification and Functional Analysis of PgSQE08-01 Gene in Ginsenoside Biosynthesis in Panax ginseng ***.

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Phyton-International Journal of Experimental Botany 2024年第2期93卷 313-327页

作者： Lei Zhu Lihe Hou Yu zhang Yang Jiang Yi Wang meiping zhang Mingzhu Zhao Kangyu Wang College of Life Science Jilin Agricultural UniversityChangchun130118China Jilin Engineering Research Center Ginseng Genetic Resources Development and Utilization Changchun130118China Jilin Academy of Vegetable and Flower Sciences Changchun130033China

Panax ginseng *** an important plant species used in traditional Chinese medicine,whose primary active ingredient is a *** biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural ***,the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary *** epoxidase(SQE)is a key enzyme in the mevalonic acid pathway,which affects the biosynthesis of secondary metabolites such as *** ginseng transcriptome,expression,and ginsenoside content databases,this study employed bioinformatic methods to systematically analyze the genes encoding SQE in *** first selected six PgSQE candidates that were closely involved in ginsenoside biosynthesis and then identified PgSQE08-01 to be highly associated with ginsenoside ***,we constructed the overexpression vector pCAMBIA3301-PgSQE08-01 and the RNAi vector pART27-PgSQE08-01 and transformed ginseng adventitious roots using Agrobacterium rhizogenes,to obtain positive hairy-root ***,quantitative reverse transcriptionpolymerase chain reaction and high-performance liquid chromatography were used to determine the expression of relevant genes and ginsenoside content,***,we focused on the function of PgSQE08-01 gene,which was noted to be involved in ginsenoside ***,these findings not only provided a molecular basis for the identification of important functional genes in ginseng but also enriched genetic resources for the biosynthesis of ginsenosides using synthetic biology.

关键词： Panax ginseng pgSQE08-01 gene squalene epoxidase ginsenoside ginseng hairy roots

Response of the Ginseng C_(2)H_(2)-Type Zinc Finger Protein Family PgZFPs Gene to Methyl Jasmonate Regulation

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Phyton-International Journal of Experimental Botany 2024年第11期93卷 3055-3071页

作者： Yue Jiang Lingyu Liu Kangyu Wang Mingzhu Zhao Ping Chen Jun Lei Yanfang Wang meiping zhang Yi Wang Guang Chen College of Life Science Jilin Agricultural UniversityChangchun130118China Research Center for Ginseng Genetic Resources Development and Utilization Jilin ProvinceJilin Agricultural UniversityChangchun130118China College of Chinese Medicinal Materials Jilin Agricultural UniversityChangchun130118China

The main active components of ginseng are ginsenosides,which play signiﬁcant roles in treating cardiovascular diseases,cancer,and providing antioxidant *** are primarily synthesized through the mevalo-nate pathway and the methylerythritol phosphate *** key enzyme genes involved in this biosynthetic process have been cloned and validated,yet the regulatory functions of transcription factors remain *** C_(2)H_(2)-type zincﬁnger protein family,one of the largest families of transcription factors,is crucial in plant growth and development,response to biotic and abiotic stresses,and regulation of secondary *** study,based on the ginseng transcriptome database from Jilin,conducted a correlation analysis between the expression levels of PgZFPs genes in the Jilin ginseng C_(2)H_(2)-type zincﬁnger protein family and ginsenoside content,a gen-ome-wide association study of PgZFPs,and co-expression analysis of PgZFPs with validated key enzyme ***,ﬁve candidate genes involved in ginsenoside biosynthesis were identiﬁ*** involvement of PgZFP27 and PgZFP-59-02 genes from the PgZFPs family in the biosynthesis of ginsenosides was validated through in vitro methyl jasmonate(MeJA)induction *** result provides new genetic resources for the biosynthesis of ginsenosides.

关键词： Ginsenosides C_(2)H_(2)-type zincﬁnger protein family MeJA induction functional study SIT SITIENS FLC FLACCA MVA Mevalonate MEP 2-C-methyl-D-erythritol 4-phosphate GWAS Genome-wide association studies HPLC High-performance liquid chromatography

Plant Diversity of Maoershan National Forest Park

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Journal of Landscape Research 2019年第5期11卷 109-111,115页

作者： zhang meiping CONG Mingyang HAN Wenge GUO Xiaohong Heilongjiang Bayi Agricultural University

There are a wide variety of wild plants in Maoershan National Forest *** to a fiveyear survey,the plants are found to include 557 species belonging to 116 families and 335 *** are 10 dominant families,such as Asteraceae,Ranunculaceae,Rosaceae,Fabaceae,and Chenopodiaceae,including 251 species,accounting for 46.06% of the *** are 9 dominant genera,such as Polygonum,Potentilla ,Viola ,Artemisia ,and Corydalis ,which include 82 species of plants,accounting for 14.72% of the *** study statistically analyzed the diversity and function of plants in the park and classified useful plants to provide a basis for the conservation and development of plant resources in Maoershan National Forest Park.

关键词： Plant resources Species diversity Evaluation Maoershan National Forest Park

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中文图书馆藏建设的纸电融合趋势初探

大学图书馆学报 2021年第1期39卷 44-49页

作者：张美萍北京大学图书馆北京100871

在新媒体及融合出版态势下,中文图书出版数量剧增、单价上涨,读者对图书的多元使用需求以及馆藏空间变化等一系列因素,促使图书馆在中文图书馆藏建设中必须设计转型发展的方案,尤其在此次疫情防控期间,读者在中文图书的使用中所暴露的问... 详细信息

在新媒体及融合出版态势下,中文图书出版数量剧增、单价上涨,读者对图书的多元使用需求以及馆藏空间变化等一系列因素,促使图书馆在中文图书馆藏建设中必须设计转型发展的方案,尤其在此次疫情防控期间,读者在中文图书的使用中所暴露的问题,要求图书馆认清方向,理清思路,以顺势求变、顺势应变,加快构建纸电融合的馆藏建设体系,更好地促使图书馆服务转型。

关键词：中文图书纸电融合馆藏建设采访模式未来趋势

Design and Application of Electronic Atlas of Ornamental Flora in the Daqing Area

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Journal of Landscape Research 2020年第2期12卷 106-110页

作者： zhang meiping GUO Xiaohong HE Lin HAN Wenge JIANG Sujun Heilongjiang Bayi Agricultural University DaqingHeilongjiang 163319China

The electronic atlas of ornamental flora in the Daqing area selects 265 species of plants from 84 *** video studio X5 software is used to edit and process pictures and text materials,and professional templates are used to make a media playback video combining picture materials,text description,background music,and subtitle *** plant is provided with a Latin scientific name,Chinese name,taxonomic status,habitat,application and other morphological characteristics description and identification *** paper expounds on the design idea and the whole production process of the *** atlas is easy to search and browse and can provide a necessary reference for gardeners,botany lovers and students to know ornamental plants correctly,and to research,develop and utilize ornamental plants.

关键词： Ornamental plants Atlas Video studio X5 Daqing

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基于持续关注度衰减的重要论文预测

复杂系统与复杂性科学 2015年第3期12卷 77-84页

作者：张美平尚明生电子科技大学互联网科学中心成都611731

针对现有方法存在预测精度较低或者计算复杂等问题,通过真实数据集的实证分析,发现论文的年均引用次数与论文未来的被引次数有很大的相关性,由此提出持续关注度的概念。进一步,结合论文引用的时间衰减特性,提出一种基于持续关注度衰减... 详细信息

针对现有方法存在预测精度较低或者计算复杂等问题,通过真实数据集的实证分析,发现论文的年均引用次数与论文未来的被引次数有很大的相关性,由此提出持续关注度的概念。进一步,结合论文引用的时间衰减特性,提出一种基于持续关注度衰减的重要论文预测算法。在两个典型数据集上的实验结果表明,该方法不仅计算简单,而且具有较高的预测精度。

关键词：持续关注度时间衰减论文引用预测

北京大学图书馆中文图书馆藏建设的变迁及未来发展探讨

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大学图书馆学报 2016年第3期34卷 85-89页

作者：张美萍北京大学图书馆资源建设中心北京100871

通过对2000-2014年北京大学图书馆中文图书馆藏建设内外环境变化的分析.梳理了近15年来中文图书馆藏建设的方式、内容及共建共享体系等的变迁和发展.指出存在的问题,探讨了数字环境下中文图书馆藏建设的发展方向。

关键词：北京大学图书馆中文图书资源建设历史回顾馆藏发展

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北京大学图书馆图书招标工作的过程及特点

大学图书馆学报 2008年第1期26卷 19-22页

作者：张美萍北京大学图书馆北京 100871

介绍北京大学图书馆是如何开展内地版中外文图书招标工作的,分析了三个工作阶段的各自特点。

关键词：大学图书馆图书采购招标北京大学

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傅兰雅与益智书会的译名统一与标准化

外国语文 2020年第2期36卷 121-127页

作者：张美平浙江树人大学人文与外国语学院浙江杭州310015

中国的发展和进步不仅取决于坚船利炮等有形力量,还取决于知识与技术的获取。翻译是获取知识与技术的基本路径。正确的译名,在实现西学移植、通过西学知识达成科技转移等方面起着重要的作用。益智书会是近代中国重要的新知识、新思想的... 详细信息

中国的发展和进步不仅取决于坚船利炮等有形力量,还取决于知识与技术的获取。翻译是获取知识与技术的基本路径。正确的译名,在实现西学移植、通过西学知识达成科技转移等方面起着重要的作用。益智书会是近代中国重要的新知识、新思想的温床和集散中心。傅兰雅以其为平台,启动并参与了译名统一与标准化工作,取得了不俗成绩。由于社会、语言、文化等方面的原因,此项工作与中国的实际需求仍存在差距,但他所做的基础性和开创性的工作,为后来由国人主导的科技译名的厘定与统一奠定了基础。

关键词：傅兰雅益智书会译名统一与标准化