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Decreased soil pH weakens the positive rhizosphere effect on denitrification capacity

Pedosphere 2024年第5期34卷 905-915页

作者： Jun WANG Zongxiang zhang Fei LIANG Zhao CHE Yongkang WEN manyu zhang Wenjun JIN Zhaorong DONG He SONG College of Agronomy Anhui Agricultural University Key Laboratory of Arable Land Quality Monitoring and Evaluation Ministry of Agriculture and Rural Affairs Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences (CAAS) College of Resources and Environmental Sciences China Agricultural University

The stimulating effect of rhizosphere on denitrification is considered to be an unavoidable loss of soil nitrogen（N） and detrimental to crop N use efficiency,which is regulated by crop growth and soil properties. Soil acidification, occurring rapidly in many intensive farming lands, affects both crop growth and soil properties, thereby altering rhizosphere effect on denitrification. However, the mechanism by which soil acidification regulates rhizosphere denitrification still remains unclear. Here, we determined the denitrification capacity（DC） and associated community compositions of nirK-and nirS-type denitrifiers in maize rhizosphere and bulk soils at four acidity gradients（pH = 6.8, 6.1, 5.2, and 4.2）. Results showed that the stimulating effect of rhizosphere on DC strongly depended on soil pH. Compared to bulk soil, rhizosphere soil had significantly higher DC at pH 5.2, but not at pH of 4.2. With increasing soil acidity, the stimulation of rhizosphere on DC（calculated as the difference in DC between rhizosphere and bulk soils） decreased from 8.01 to 0.01 mg N ***, soil acidification significantly reduced the differences in dissolved organic carbon（DOC） and abundance of key nirK-type denitrifier taxa between rhizosphere and bulk soils, both of which were positively related to the stimulation of rhizosphere on DC. These findings demonstrated that soil acidification could weaken the positive rhizosphere effect on denitrification via regulated C availability and associated nirK-type denitrifier community, potentially reducing N loss risk in rhizosphere soil. The independent role of soil p H should be fully considered when modelling N behaviour in plant-soil systems.

关键词： bacterial abundance denitrifier community dissolved organic carbon operational taxonomic unit soil acidification

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Phosphorylation of PB2 at serine 181 restricts viral replication and virulence of the highly pathogenic H5N1 avian influenza virus in mice

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Virologica Sinica 2024年第1期39卷 97-112页

作者： Jiao Hu Zixiong Zeng Xia Chen manyu zhang Zenglei Hu Min Gu Xiaoquan Wang Ruyi Gao Shunlin Hu Yu Chen Xiaowen Liu Daxin Peng Xiufan Liu Animal Infectious Disease Laboratory School of Veterinary MedicineYangzhou UniversityYangzhou225009China Joint International Research Laboratory of Agriculture and Agri-Product Safety The Ministry of Education of ChinaYangzhou University225009China Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis Yangzhou UniversityYangzhou225009China Key Laboratory of Prevention and Control of Biological Hazard Factors(Animal Origin)for Agri-food Safety and Quality Ministry of Agriculture of China(26116120)Yangzhou UniversityYangzhou225009China

Influenza A virus(IAV)continues to pose a pandemic threat to public health,resulting a high mortality rate annually and during pandemic *** modification of viral protein plays a substantial role in regulating IAV ***,based on immunoprecipitation(IP)-based mass spectrometry(MS)and purified virus-coupled MS,a total of 89 phosphorylation sites distributed among 10 encoded viral proteins of IAV were identified,including 60 novel phosphorylation ***,for the first time,we provide evidence that PB2 can also be acetylated at site ***,the PB2 S181 phosphorylation site was consistently identified in both IP-based MS and purified virus-based *** S181 and K187 are exposed on the surface of the PB2 protein and are highly conserved in various IAV strains,suggesting their fundamental importance in the IAV life *** analysis results demonstrated that S181E/A and K187Q/R mimic mutations do not significantly alter the PB2 protein *** continuous phosphorylation mimicked by the PB2 S181E mutation substantially decreases viral fitness in mice,PB2 K187Q mimetic acetylation slightly enhances viral virulence in ***,PB2 S181E substantially impairs viral polymerase activity and viral replication,remarkably dampens protein stability and nuclear accumulation of PB2,and significantly weakens IAV-induced inflammatory ***,our study further enriches the database of phosphorylation and acetylation sites of influenza viral proteins,laying a foundation for subsequent mechanistic ***,the unraveled antiviral effect of PB2 S181E mimetic phosphorylation may provide a new target for the subsequent study of antiviral drugs.

关键词： H5N1 influenza virus PB2 Phosphorylation Acetylation Viral fitness Mice

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Bird Species Diversity and Spatio-temporal Variation in the Sihong Hongze Lake Wetland National Nature Reserve in Eastern China

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Journal of Resources and Ecology 2024年第4期15卷 826-837页

作者： HU Huali XIAO Lihui zhang manyu WANG Silu CHEN Taiyu LU Changhu College of Life Science Nanjing Forestry UniversityNanjing 210037China Eastern Hongze Lake Wetland Natural Reserve HuaianJiangsu 223100China Institute of Wildlife Conservation Jiangxi Academy of ForestryNanchang 330037China

As an important hub on the East Asian-Australian Flyway(EAAF),Hongze Lake is an important migratory stopover and wintering site for hundreds of thousands of ***,research on bird community diversity in this area is still *** conducted a bird survey from July 2020 to June 2021 using the line transect method on the terrestrial habitat,as well as the fixed-point method in the lake wetland at the Sihong Hongze Lake Wetland National Nature Reserve located in northwestern Hongze Lake,and analyzed the temporal-spatial variation in the bird *** results showed that a total of 170432 detections of 215 bird species belonging to 18 orders and 55 families were *** terms of species composition,the proportion of terrestrial birds was relatively high,followed by waterfowl richness,with high numbers of Anatidae species and *** bird species abundance,waterfowl had the highest abundance of common coot(Fulica atra),belonging to the Rallidae family,followed by Anatidae and Ardeidae *** abundance of shorebirds was extremely low,and that of non-waterfowl was also *** terms of temporal patterns,the number of bird species and richness index were higher in spring and autumn but lower in winter and *** bird abundance was the highest in winter,and the remaining three seasons were similar in terms of bird *** diversity index and evenness index were higher in spring,summer and autumn,and lowest in *** the spatial pattern,the open water in the western part of the reserve included the most densely distributed areas for birds,and the number of bird species and their abundance were both the highest in that *** were significant differences in the bird community structures among the four *** species number and richness index of birds in the reed habitat were the highest,and the bird abundance was also high,but the diversity index and evenness index were *** the bird abundance in the lake habitat wa

关键词： bird diversity seasonal variation spatial distribution Sihong Hongze Lake Wetland National Nature Reserve

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自我控制对大学新生学校适应的影响:应对方式的中介作用

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心理学通讯 2024年第1期7卷 79-87页

作者：张曼玉封慰泰州学院心理健康发展中心泰州225300 江苏食品药品职业技术学院心理发展服务中心淮安223023

目的:探讨自我控制对大学新生学校适应的影响及应对方式的中介作用。方法:采用中国大学生适应量表(Chinese College Student Adjustment Scale,CCSAS)、自我控制量表(Self-Control Scale,SCS)和简易应对方式问卷(Simplified Coping Style Questionnaire,SCSQ)调查了江苏省1675名大学新生。结果:(1)自我控制、积极应对、学校适应两两之间呈正相关(自我控制与积极应对:r=0.475,p<0.001;自我控制与学校适应:r=0.708,p<0.001;积极应对与学校适应:r=0.632,p<0.001);消极应对与自我控制、学校适应呈负相关(自我控制与消极应对:r=-0.325,p<0.001;消极应对与学校适应:r=-0.218,p<0.001)。(2)自我控制不仅能直接影响大学新生的学校适应,还能通过积极应对和消极应对方式对学校适应产生间接影响,间接效应占总效应的28.5%。结论:自我控制和应对方式是大学新生学校适应的重要影响因素。积极应对和消极应对在自我控制与大学新生学校适应之间起中介作用。

关键词：学校适应自我控制积极应对消极应对大学新生

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基于随机森林模型的安徽省三级甲等医院临床护士循证护理能力现状及影响因素分析

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中国实用护理杂志 2024年第18期40卷 1395-1402页

作者：许栋王茜徐贵霞赵龙张曼玉王一新蚌埠医科大学护理学院蚌埠233030 蚌埠医科大学第一附属医院护理部蚌埠233004

目的了解安徽省三级甲等医院临床护士循证护理能力现状,基于随机森林模型分析其影响因素,为制订临床护士循证护理能力干预策略提供参考。方法采用便利抽样法,于2022年10—12月,对安徽省4所三级甲等医院550名临床护士采用一般资料调查表... 详细信息

目的了解安徽省三级甲等医院临床护士循证护理能力现状,基于随机森林模型分析其影响因素,为制订临床护士循证护理能力干预策略提供参考。方法采用便利抽样法,于2022年10—12月,对安徽省4所三级甲等医院550名临床护士采用一般资料调查表、循证护理能力评定量表、信息素养量表、临床护理人员创新能力评价量表进行横断面调查,利用随机森林模型对变量进行重要性评价,应用套索回归分析完成变量筛选,通过逐步多元线性回归分析探究临床护士循证护理能力的影响因素。结果回收有效问卷543份。543名临床护士中男55名,女488名,年龄(32.34±6.93)岁,循证护理能力总分为(45.49±21.18)分,信息素养总分为(73.50±10.47)分,创新能力总分为(126.78±21.99)分。随机森林模型和套索回归分析显示,变量数为8时模型拟合最佳,重要性排序位于前8位的变量依次为信息素养(25.78%)、创新能力(22.37%)、每月夜班数(9.91%)、学历(9.19%)、英语水平(8.44%)、职称(6.71%)、有无科研创新经历(5.17%)、职业态度(4.50%)。逐步多元线性回归分析显示,信息素养、创新能力、英语水平是临床护士循证护理能力的主要影响因素(t=9.17、7.31、2.52,均P<0.05)。结论安徽省三级甲等医院临床护士循证护理能力水平有待提升。护理管理者可从提升临床护士信息素养层面出发,加大信息检索能力与英语能力的培训,增强其英文文献阅读技巧,并重视临床护士创新能力的培养,激发临床护士的创新意识与思维,制订并实施针对性干预措施,以逐渐提高临床护士循证护理能力水平。

关键词：护士循证护理能力随机森林模型影响因素

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Behaviors of Leucine and Isoleucine in Ion Mobility- Quadrupole Time of Flight Mass Spectrometry

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Chinese Journal of Chemistry 2015年第12期33卷 1359-1364页

作者： Su Guo Fang zhang Haoyang Wang manyu zhang Zhixu zhang Xiang zhang Yinlong Guo National Center for Organic Mass Spectrometry in Shanghai Shanghai Institute of Organic Chemistry Chinese Academy of Sciences 345 Lingling Road Shanghai 200032 China Agilent Technologies (China) CO. Ltd. 1350 North Sichuan Road Shanghai 200080 China Department of Chemistry Fudan University 220 Handan Road Shanghai 200433 China School of Food Science and Biotechnology Zhejiang Gongshang University Hangzhou Zhejiang 310018 China

In this study, ion mobility separation coupled with tandem mass spectrometry （IM-tandem MS） was utilized to investigate the ionization behaviors of two amino acids including leucine and isoleucine. Under the electrospray ionization （ESI） mode, two protonation sites in each molecular sturcture caused two forms of protomer. One arose from the amino being protonated （amino-protomer） and the other from the carboxyl being protonated （car- boxyl-protomer）. In the two-dimensional （drift time, m/z） spectrum, the protomers had the same mass, but the dis- tinguishable drift times and fragmentation patterns. For the characterization purpose, the theoretical collision cross section （CCS） values of the protomers were calculated and proven to be consistent with the experimental. Moreover, the quantified relationship between the amino acids and their protomers was evaluated. It showed that the abun- dance of the carboxyl-protomer was proportional to the concentration of the amino acid, whereas that of the amino- protomer did not have the same trend. Under the atmospheric pressure chemical ionization （APCI） mode, only the carboxyl-protomer was observed. In addition, the amino-protomer and the cluster ions observed under ESI were absent completely. The results demonstrate that the ionization mode impacts heavily on the ionization behaviors of leucine and isoleucine not only on the form of therir protomers but also On the quantified relationship.

关键词： leucine isoleucine IM-tandem MS ESI APCI

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Song variation of a native songbird in a modified habitat by invasive plant

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Integrative Zoology 2022年第1期17卷 93-104页

作者： Pan CHEN Taiyu CHEN Bin LIU manyu zhang Changhu LU College of Biology and the Environment Nanjing Forestry UniversityNanjingChina School of Ecology and Environment Anhui Normal UniversityChina

Habitat structure has been considered as an important factor affecting the acoustic evolution of birds,and bird songs are increasingly affected by artificial environmental *** plants sometimes can dramatically alter native habitats,but the song variation of native songbirds migrating into invaded habitats has received little *** invasion of smooth cordgrass Spartina alterniflora in the coastal wetlands of eastern China has drastically altered the vegetation structure and some small passerines have begun to use invaded habitats to *** this study,we compared the song type prevalence and the song characteristics of male plain prinia Prinia inornata to identify differences in vocal behavior between native and invaded *** also tested for differences in vocal behavior in relation to singing perch and wind speed variation between different *** results indicated that males of plain prinia in invaded habitats sang shorter songs than those in native habitats and had a lower song *** homogeneous vegetation structure and higher wind speed in invaded habitats likely leads to males changing the traditional perched singing *** song variation may be related to the founder effect,the alteration of vegetation structure and microclimate in invaded *** finding highlights the need for better understanding the behavioral evolution of native species in the process of adapting to the invaded *** the future,experimental manipulation is needed to ascertain how the invasive plant drove these vocal behavior changes of native songbirds.

关键词： behavioral evolution biological invasion plain prinia smooth cordgrass song diversity

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质谱仪离子源和质谱仪质量分析器的生产制造工艺

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模具制造 2024年第2期24卷 232-234页

作者：刘传兴张曼玉杲立博牛天渭青岛惠安康生物工程有限公司山东青岛266114

质谱仪离子源和质谱仪质量分析器能够将化学样品分析为其组成部分的离子,并根据其质量-电荷比进行分离和检测,是许多关键领域不可或缺的分析工具。剖析质谱仪离子源和质谱仪质量分析器的生产制造工艺,通过解决当前面临的技术难题、改进... 详细信息

质谱仪离子源和质谱仪质量分析器能够将化学样品分析为其组成部分的离子,并根据其质量-电荷比进行分离和检测,是许多关键领域不可或缺的分析工具。剖析质谱仪离子源和质谱仪质量分析器的生产制造工艺,通过解决当前面临的技术难题、改进工艺流程以及推动新技术的创新发展,以有效提高质谱仪离子源和质谱仪质量分析器的制造质量和性能,为相关领域的科学研究和应用提供更加可靠和先进的分析工具。

关键词：质谱仪离子源质谱仪质量分析器生产制造工艺

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Vinous-throated parrotbills breed in invasive smooth cordgrass habitat:Can native birds avoid the potential ecological trap?

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Avian Research 2023年第3期14卷 369-376页

作者： Pan Chen Yanhong Chen Huimin Chen Taiyu Chen Bin Liu manyu zhang Silu Wang Changhu Lu Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources College of Life SciencesAnhui Normal UniversityWuhu241000China College of Biology and Environment Nanjing Forestry UniversityNanjing210037China

Native animals are facing long-term coexistence with invasive plants worldwide,the impacts of which on animal behavior remain poorly *** ecological traps could threaten native birds breeding in invasive plant habitats,but behavioral strategies for birds to avoid such risks are few *** invasion of Smooth Cordgrass(Spartina alterniflra) has seriously varied the vegetation landscape in the coastal wetlands of eastern China,and affected the habitat selection of native ***,we investigated the nesting and breeding characteristics of a common native passerine,the Vinous-throated Parrotbill(Sinosuthora webbiana),in habitats dominated by native Common Reed(Phragmites australis) and exotic *** found that parrotbills could complete their breeding cycle in cordgrass *** nest failure was attributed to predation in native habitat but tide inundation in cordgrass ***,the nest success rate and daily survival rate(DSR) in cordgrass habitat were ***,nest height was markedly higher in cordgrass habitat and was the most important influencing variable(positively correlated with the DSR).These results suggest that cordgrass habitat is a potential ecological trap due to the tide inundation,but some parrotbills seem to effectively avoid this risk by increasing nest *** study reveals that a native passerine changes its nesting behavior to accommodate invasive plant habitat and highlights that habitat changes caused by invasive plants may drive the adaptive evolution of native animal *** limitation of these results must be acknowledged for the small sample size,and there is a need for a larger sample and long-term data for further verification.

关键词： Adaptive evolution Behavioral plasticity Nesting behavior Sinosuthora webbiana Spartina alterniflora

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江西省爬行动物新记录——古氏草蜥

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南方林业科学 2024年第2期52卷 77-78页

作者：张曼玉张壹萱吴怀远韩卫杰黄晓凤江西省林业科学院江西南昌330013 新余市林业局江西新余338099

2022年7月,在江西省新余市大岗山进行爬行动物调查时拍摄到一种蜥蜴,经形态鉴定确认为古氏草蜥(Takydromus kuehnei),是江西省爬行动物新记录。

关键词：江西省爬行动物新记录古氏草蜥

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