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Effects of Cu^(2+) on characteristic of SV currents

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Science China Chemistry 2006年第6期49卷 560-564页

作者： zhang liping1,2.& yang pin1 1. key laboratory of chemical biology and molecular engineering of ministry of education, Institute of molecular Science, Shanxi University, Taiyuan 030006, China 2. Institute of Environment Science, Taiyuan University of Science and Technology, Taiyuan 030024, China key laboratory of chemical biology and molecular engineering of ministry of education Institute of Molecular Science Shanxi University Taiyuan China Institute of Environment Science Taiyuan University of Science and Technology Taiyuan China

Applying the patch-clamp technique to vacuoles from Radish we studied the effects of Cu2+ on Slow Vacuolar (SV) current’s characteristic. Our results show that Cu2+ in bath solution at higher concentration inhibits SV currents and the percentage of inhibition increases with increasing concentration and changes with different voltage. When at lower concentration, Cu2+ significantly promotes the SV currents and the promotion ratio decrease with increasing voltage. At the same time, the time constants of activation become lesser after adding Cu2+. These results show that there may be some Cu2+ binding sites on SV channels and binding to which can change SV current’s characteristic.

关键词： the patch-clamp technique, vacuolar, Cu2+, SV channel.

Light-Mediated Spatiotemporally Dynamic Assembly of DNA Nanostructures in Living Cells Regulates Autophagy

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CCS Chemistry 2024年第6期6卷 1557-1570页

作者： Rui zhang pin Hu Yuwei Xu Ziqing Wang Dayong yang Chi Yao Frontiers Science Center for Synthetic biology Key Laboratory of Systems Bioengineering(MOE)School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350 Department of Chemistry State Key Laboratory of Molecular Engineering of PolymersShanghai Key Laboratory of Molecular Catalysis and Innovative MaterialsCollege of Chemistry and MaterialsFudan UniversityShanghai 200438

The assembly of exogenous artificial architectures inside cells can regulate a series of biological events,which heavily relies on the development of spatiotemporally controlled molecular assembly *** herein report a designer deoxyribonucleic acid(DNA)nanostructure that enables light-mediated spatiotemporally dynamic assembly in living cells and consequently achieves efficient regulation of cell *** DNA nanostructure was constructed from i-motif moiety-containing branched DNA,photocleavable bond-containing linker,and tumor cell-targeting *** cellular uptake mediated by aptamers,under the spatiotemporal control of both UV light and late endosomal/lysosomal acidic environments,disassembly/reassembly of DNA nanostructure occurred via two rationally designed routes,generating microsized DNA *** a result,autophagy was significantly enhanced with the increase of DNA assembly *** enhanced autophagy showed an impact on related biological *** system is expected to be a powerful tool for the regulation of intracellular events and cellular behaviors.

关键词： DNA nanotechnology DNA hydrogel self-assembly autophagy i-motif

Structures and Properties of Cytosine-BX3（X=F,Cl） Complexes: An Investigation with DFT and MP2 Methods

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Structures and Properties of Cytosine-BX3（X=F,Cl） Complexe...

Effects of ytterbium on outward K^+ currents in NIH3T3 cell

中国化学会第九届全国量子化学学术会议

作者： Shiguo zhang,pin yang key laboratory of chemical biology and molecular engineering of ministry of education, Institute of molecular Science, Shanxi University, Taiyuan 030006 Department of Computer Science and Technology, Binzhou University, Binzhou 256600

＜正＞For the cytosine-BX3（X=F,Cl） systems, geometries and binding energies were calculated using the B3LYP method of DFT and MP2 method of ab initio with 6-311+G* or 6-311++G* bases sets. Three isomers were found for each system, and then single-point energy evaluations were performed using the larger basis sets (6-311+G（2df,p） with DFT method. In the most stable isomers of cytosine-BF3 or cytosine-BCl3, boron atom of BX3（X=F,Cl） connected to carbonyl oxygen O8 of cytosine with a stabilization energy of -78.71 and -69.62 kJ/mol at B3LYP/6-311+G* level (BSSE

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Chinese Science Bulletin 2010年第3期55卷 246-250页

作者： zhang liping CHEN Ming yang pin key laboratory of chemical biology and molecular engineering of ministry of education Institute of Molecular Science Shanxi University Taiyuan 030006 China Institute of Environment Science Taiyuan University of Science and Technology Taiyuan 030024 China Institute of Life Sciences Taishan Medical College Tai'an 271000 China

Using the whole cell patch-clamp technique, we observed the outward K+ currents and studied for the first time the effects of Yb3+ on the currents and kinetics of activation and inactivation in non-excitable NIH3T3 cell. Our results show that the outward K+ currents were promoted with increasing concentration of Ca2+ in pipette solution and saturated at the concentration of 100 μmol/L Ca2+. Yb3+ in bath solution inhibited the currents in a concentration-dependent manner. At the concentration of 1 μmol/L, Yb3+ significantly changed the Vh of activation curve and shifted the activation curve to more negative potentials, but with no effect on its slope factor k. Meanwhile, it had no effect on the inactivation curve. Potassium currents inhibition can induce a series of physiological and molecular biological functions, presumably because of its ability to depolarize the plasma membrane and enhance cell excitability resulting in increasing Ca2+ influx and cytoplast Ca2+ concentration. This process may be one of the mo- lecular mechanisms to affect cell division and proliferation of Yb3+ on NIH3T3 cells.

关键词： NIH3T3细胞钾电流全细胞膜片钳技术镱饱和浓度浓度依赖性生物学功能胞质钙

Study of the interaction of unaggregated and aggregated amyloid β protein (10-21) with outward potassium channel

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Science China Chemistry 2007年第5期50卷 683-691页

作者： zhang ChaoFeng FAN Li yang pin key laboratory of chemical biology and molecular engineering of ministry of education Institute of Molecular Science Shanxi University Taiyuan 030006 China Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of Molecular Science Shanxi University Taiyuan 030006 China Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of Molecular Science Shanxi University Taiyuan 030006 China

Metal ion-induced aggregation of A beta into insoluble plaques is a centralfactor in Alzheimer's ***^(2+) is the only physiologically available transition metal ionresponsible for aggregating A beta at pH *** make it clear that the neurotoxicity ofZn^(2+)-induced aggregation of A beta on neurons is the key to understand A beta mechanism of *** this paper,we choose A/?(10-21) as the model fragment to research hippocampal CA1pyramidal *** the first time,we adopt the combination of spectral analysis with patch-clamptechnique for the preliminary study of the mutual relations of Zn^(2+),A beta and ion channel fromthe cell *** following expounds upon the effects and mode of action of two forms (unaggregatedand aggregated) of A beta(10-21) on hippocampus outward potassium channel three processes(activation,inactivation and reactivation).It also shows the molecular mechanics of AD from thechannel *** results are significant for the further study of A beta nosogenesis and thedevelopment of new types of target drugs for the treatment of AD.

关键词： potassium channel amyloid beta protein (10-21) Zn^(2+) alzheimer's disease

molecular modeling on the recognition of DNA sequence and conformational repair of sheared DNA by novel chiral metal complex D, L-[Co(phen)_(2)hpip]^(3+)

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Science China Chemistry 2006年第2期49卷 177-185页

作者： WU Yanbo zhang Cuiping yang pin Institute of molecular Science Key Laboratory of Chemical Biology of Molecular Engineering of Ministry of Education Shanxi University Taiyuan 030006 China Institute of Molecular Science Key Laboratory of Chemical Biology of Molecular Engineering of Ministry of Education Shanxi University Taiyuan 030006 China Institute of Molecular Science Key Laboratory of Chemical Biology of Molecular Engineering of Ministry of Education Shanxi University Taiyuan 030006 China

A study on the recognition of DNA sequence and conformational repair of sheared DNA by Novel Chiral Metal complex D,L-[Co(phen)2hpip]3+ (phen=1,10 phenanthroline, hpip=2-[2-hydroxyphenyl] imidazole [4,5-f][1,10] phenanthroline) is carried out with molecular simula-tions. The results reveal that two isomers of the complex could both recognize the normal DNA in the minor groove orientation, while recognize the sheared DNA in the major groove orientation and both isomers could convert the conformation of mismatched bases from sheared form to parallel form. Further analysis shows that the steric details of complex’s intercalation to base stack determine the results of recognition, which is induced by the steric collision among ancillary ligand phen, bases and DNA backbone, and by the steric crowding occurring in the process of structural expansion of bases and DNA backbone. Detailed analysis reveals that the conformational repair of mismatched bases relates not only to the steric interactions, but also to the π-π stack among normal bases, mismatched bases and hpip ligand.

关键词： mismatched DNA, metal complex, molecular modeling, docking.

Effects of lithium chloride on outward potassium currents in acutely isolated hippocam-pal CA1 pyramidal neurons

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Chinese Science Bulletin 2006年第16期51卷 1961-1966页

作者： zhang Chaofeng DU Huizhi yang pin key laboratory of chemical biology and molecular engineering of ministry of education Institute of Molecular Science Shanxi University Taiyuan 030006 China

Although lithium possesses neuropro- tective functions, the molecular mechanism underly- ing its actions has not been fully elucidated. In the present paper, the effects of lithium chloride on volt- age-dependent potassium currents in the CA1 py- ramidal neurons acutely isolated from rat hippocam- pus were studied using the whole-cell patch-clamp technique. Depolarizing test pulses activated two components of outward potassium currents: a rapidly activating and inactivating component, IA and a de- layed component, IK. Results showed that lithium chloride increased the amplitude of IA in a concentra- tion-dependent manner. Half enhancement concen- tration (EC50) was 22.80±5.45 μmol?L?1. Lithium chloride of 25 μmol?L?1 shifted the steady-state acti- vation curve and inactivation curve of IA to more negative potentials, but mainly affected the activation kinetics. The amplitude and the activation processes of IK were not affected by lithium chloride. The effects of lithium chloride on potassium channel appear to possess neuroprotective properties by Ca2+-lowing effects modulate neuronal excitability by activating IA in rat hippocampal neurons.

关键词：氯化锂 CA1神经原钾电流 patch-clamp技术海马

Study of the interaction of Zn^(2+) with Aβ(10-21) by fluorescamine assay

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Chinese chemical Letters 2007年第1期18卷 97-98页

作者： Chao Feng zhang Li Fan pin yang key laboratory of chemical biology and molecular engineering of ministry of education Institute of Molecular Science Shanxi University Taiyuan 030006 China

Zinc may play a role as a co-factor in the pathogenesis of Alzheimer＇s disease （AD） through influencing the conformation and neurotoxicity of amyloid β-protein （Aβ）. Using the fluorescamine assay, we show for the first time that Zn^2＋ induced Aβ（10-21） aggregate in a concentration-dependent manner. These results indicate that Aβ（10-21） can be used as an in vitro model in Zn^2＋ - induced Aβ aggregation and that the region 10-21 to be the minimal fragment of zinc-binding domain of full length Aβ（1-42）.

关键词： Aβ(10-21) Fluorescamine Zn^2+ Aggregation

engineering a customized nanodrug delivery system at the cellular level for targeted cancer therapy

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Science China Chemistry 2018年第4期61卷 497-504页

作者： Jin Li liping Qiu Sitao Xie Jing zhang Liqin zhang Honglin Liu Juan Li Xiaobing zhang Weihong Tan molecular Science and Biomedicine laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of Life SciencesAptamer Engineering Center of Hunan ProvinceHunan University Center for Research at Bio/Nano Interface Department of Chemistry and Department of Physiology and Functional GenomicsHealth Cancer CenterUF Genetics Institute and McKnight Brain InstituteUniversity of Florida key laboratory for Analytical Science of Food Safety and biology (ministry of education) College of ChemistryFuzhou University

Drug administration customized to individual cells could intrinsically address cancer heterogeneity and provide a safe and effective method for delivering personalized treatment. To accomplish this, we developed a smart nanodrug delivery system characterized by cancer cell-targeted drug delivery and intracellular biomarker-responsive drug activation. This system was composed of a long-nicked DNA duplex formed by tandem hybridization of two extended antisense oligonucleotides whose ends were separately blocked with a cancer cell-specific aptamer, AS1411,and a replaceable anti-biomarker probe(ABP). We demonstrated that this DNA nanodrug was directed to cancer cells with the guidance power of AS 1411 and then activated by the presence of a given intracellular biomarker. By using such a belt-and-braces strategy, this DNA nanodrug system could safely and efficiently accelerate apoptosis of target cancer cells. Moreover, since the expression level of biomarkers tends to indicate the specific physiological state of individual cells, biomarker-responsive activation of the nanodrug is expected to enable customized drug administration at the cellular level.

关键词： aptamer drug delivery personalized medicine antisense oligonucleotides