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DNA barcoding:species delimitation in tree peonies

Science china(Life sciences) 2009年第6期52卷 568-578页

作者： zhang jinmei1,2.wang jianxiu1,{2.,2.& zhou shiliang1 1state key Laboratory of systematic and evolutionary botany,institute of botany,{12.,beijing 100093,china 2.raduate School of {12.,beijing 100039,china 1. state key Laboratory of {2. and evolutionary botany Institute of Botany the Chinese Academy of Sciences Beijing 100093 China 2. Graduate School of the chinese academy of sciences Beijing 100039 China

Delimitations of species are crucial for correct and precise identification of *** "spe-cies" is more a subjective than an objective concept in taxonomic practice due to difficulties in revealing patterns of infra-or inter-specific *** phylogenetic studies at the population level solve this problem and lay a sound foundation for DNA *** this paper we exemplify the necessity of adopting a phylogenetic concept of species in DNA barcoding for tree peonies(Paeonia ***).We used 40 samples representing all known populations of rare and endangered species and several populations of widely distributed tree *** currently recognized species and major variants have been included in this *** chloroplast gene fragments,***,rps16-trnQ,trnL-F and trnS-G(a total of 5040 characters,96 variable and 69 parsimony-informative characters) and one variable and single-copy nuclear GPAT gene fragment(2.93?2.97 bp,2.9 variable and 148 parsi-mony-informative characters) were used to construct phylogenetic relationships among the *** evolutionary lineages revealed by the nuclear gene and the chloroplast genes are inconsistent with the current circumscriptions of ***,***,***,and *** based on *** inconsistencies come from(1) significant chloroplast gene divergence but little nuclear GPAT gene divergence among population systems of *** + ***,and(2. well-diverged nuclear GPAT gene but little chloroplast gene divergence between *** and *** incongruence of the phylogenies based on the chloroplast genes and the nuclear GPAT gene is probably due to the chloro-plast capture event in evolutionary history,as no reproductive barriers exist to prevent inter-specific *** also evaluated the suitability of these genes for use as DNA barcodes for tree *** variability of chloroplast genes among well-defined species or population systems of a specie

关键词： DNA barcoding evolution Paeonia phylogeny species concept

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Genetic differentiation of Wolffia globosa in china

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Journal of systematics and Evolution 2011年第6期49卷 509-517页

作者： 1,2.un-xia YUAN 1Jin PAN 1Bao-Sheng wang 1Da-Ming {2. 1(state key Laboratory of systematic and evolutionary botany,institute of botany,chinese academy of sciences,{12.,china) 2.Graduate University of chinese academy of sciences,beijing 100049,china) (state key Laboratory of {2. and evolutionary botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (Graduate University of chinese academy of {2. Beijing 100049 China) *Correspondence: Da-Ming zhang *Correspondence: E-mail: zhangdm@*** Tel.: 86-10-62.36492 Fax: 86-10-62.90843.

Wolffia Horkel ex Schleid.(Lemnaceae) includes the world’s smallest ***,their bodies are extremely simplified,making classification difficult and *** reports about the genetic structure of these clonal-dominant aquatic plants have been published until *** this study,of 2.7 samples from 42.populations spanning representative locations in china,two chloroplast haplotypes(glo-cp and un-cp) and 66 amplified fragment length polymorphism genotypes were *** on wide sampling,cpDNA haplotypes,and amplified fragment length polymorphism profiles,we found that there are two genetic lineages(glo-and unlineages) of Wolffia species in *** and genetic diversity of Wolffia species are high compared with other clonal plants(Simpson’s index,D = 0.97;Nei’s diversity,H = 0.1835).Different spatial structure patterns were detected between glo-and *** autocorrelation at short distances(1500 km) were detected within the glo-lineage,but no significant spatial genetic structure was detected beyond 100 km within the ***,spatial genetic analysis of *** revealed significant autocorrelation within short distances,indicating that restricted gene flow might be one of the most important factors in shaping genetic structure.

关键词： AFLP chloroplast DNA genetic differentiation spatial genetic structure Wolffia.

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Achieving structurally stable O3-type layered oxide cathodes through site-specific cation-anion co-substitution for sodium-ion batteries

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Journal of Energy Chemistry 2024年第6期93卷 411-418,I0011页

作者： Yihao Shen Chen Cheng xiao xia Lei {2. Xi zhou Pan Zeng Jianrong Zeng Liang zhang institute of Functional Nano&Soft Materials(FUNSOM) Soochow UniversitySuzhou 215123JiangsuChina institute for Advanced Study School of Mechanical EngineeringChengdu UniversityChengdu 610106SichuanChina Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China Shanghai institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Jiangsu key Laboratory of Advanced Negative Carbon Technologies Soochow UniversitySuzhou 215123JiangsuChina

O3-type layered oxides have garnered great attention as cathode materials for sodium-ion batteries because of their abundant reserves and high theoretical ***,challenges persist in the form of uncontrollable phase transitions and intricate Na^(+)diffusion pathways during cycling,resulting in compromised structural stability and reduced capacity over *** study introduces a special approach employing site-specific Ca/F co-substitution within the layered structure of O_(3)-NaNi_(0.5)Mn_(0.5)O_(2. to effectively address these ***,the strategically site-specific doping of Ca into Na sites and F into O sites not only expands the Na^(+)diffusion pathways but also orchestrates a mild phase transition by suppressing the Na^(+)/vacancy ordering and providing strong metal-oxygen bonding strength,*** as-synthesized Na_(0.95)Ca_(0.05)Ni_(0.5)Mn_(0.5)O_(1.95)F_(0.05)(NNMO-CaF)exhibits a mild O3→O3+O'3→P3 phase transition with minimized interlayer distance variation,leading to enhanced structural integrity and stability over extended *** a result,NNMO-CaF delivers a high specific capacity of 119.5 mA h g^(-1)at a current density of 12. mA g^(-1)with a capacity retention of 87.1%after 100 *** study presents a promising strategy to mitigate the challenges posed by multiple phase transitions and augment Na^(+)diffusion kinetics,thus paving the way for high-performance layered cathode materials in sodium-ion batteries.

关键词： Sodium-ion batteries O3-type layered oxides Site-specific co-doping Phase transition

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Hyphae-mediated bioassembly of carbon fibers derivatives for advanced battery energy storage

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Carbon Energy 2024年第6期6卷 140-150页

作者： Lei Huang Zhong Qiu Ping Liu Xinhui xia Feng Cao Xinping He Chen wang wangjun Wan Yongqi zhang Yang xia Wenkui zhang Minghua Chen Jiancang zhou Department of Critical Care Medicine Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhouChina state key Laboratory of Silicon Materials Key Laboratory of Advanced Materials and Applications for Batteries of Zhejiang ProvinceSchool of Materials Science&EngineeringZhejiang UniversityHangzhouChina College of Materials Science&Engineering Zhejiang University of TechnologyHangzhouChina state key Laboratory of Photocatalysis on Energy and Environment Fuzhou UniversityFuzhouChina Department of Engineering Technology Huzhou CollegeHuzhouChina Zhejiang academy of Science and Technology for Inspection&Quarantine HangzhouZhejiangChina institute of Fundamental and Frontier Science University of Electronic Science and Technology of ChinaChengduChina key Laboratory of Engineering Dielectric and Applications(Ministry of Education) School of Electrical and Electronic EngineeringHarbin University of Science and TechnologyHarbinChina

Ingenious design and fabrication of advanced carbon-based sulfur cathodes are extremely important to the development of high-energy lithium-sulfur batteries,which hold promise as the next-generation power ***,for the first time,we report a novel versatile hyphae-mediated biological assembly technology to achieve scale production of hyphae carbon fibers(HCFs)derivatives,in which different components including carbon,metal compounds,and semiconductors can be homogeneously assembled with HCFs to form composite *** mechanism of biological adsorption assembly is also *** a representative,reduced graphene oxides(rGOs)decorated with hollow carbon spheres(HCSs)successfully co-assemble with HCFs to form HCSs@rGOs/HCFs hosts for sulfur *** this unique architecture,not only large accommodation space for sulfur but also restrained volume expansion and fast charge transport paths are ***,multiscale physical barriers plus chemisorption sites are simultaneously established to anchor soluble lithium ***,the designed HCSs@rGOs/HCFs-S cathodes deliver a high capacity(1189 mA h g^(-1)at 0.1 C)and good high-rate capability(686 mA h g^(-1)at 5 C).Our work provides a new approach for the preparation of high-performance carbon-based electrodes for energy storage devices.

关键词： bioassembly carbon fibers energy storage graphene lithium-sulfur batteries

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Piperazine-Assisted Construction of 2./3D Wide-Bandgap Perovskite for Realizing High-Efficiency Perovskite/Organic Tandem Solar Cells

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chinese Journal of Chemistry 2024年第16期42卷 1819-1827页

作者： Ziyue wang Shuaiqing Kang xia {2. Haiyang Chen Xingxing Jiang Zhichao zhang Jialei Zheng Ruopeng zhang Weijie Chen Jiandong zhang Yaowen Li Laboratory of Advanced Optoelectronic Materials Suzhou Key Laboratory of Novel Semiconductor-optoelectronics Materials and DevicesCollege of ChemistryChemical Engineering and Materials ScienceSoochow UniversitySuzhouJiangsu 215123China Jiangsu key Laboratory of Advanced Negative Carbon Technologies Soochow UniversitySuzhouJiangsu 215123China state and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials Jiangsu Key Laboratory of Advanced Functional Polymer Design and ApplicationCollege of ChemistryChemical Engineering and Materials ScienceSoochow UniversitySuzhouJiangsu 215123China College of Physics and Electronics Engineering Hengyang Normal UniversityHengyangHunan 421002China

Monolithic perovskite/organic tandem solar cells(TsCs)have gained significant attention due to their easy device integration and the potential to surpass the Shockley-Queisser limit of single-junction solar ***,the surfaces of wide-bandgap perovskite films are densely populated with defects,leading to severe non-radiative recombination and energy *** a consequence,the power conversion efficiency(PCE)of perovskite/organic TSCs lags behind that of other TSC *** address these issues,we designed a functional ammonium salt,4-(2.hydroxyethyl)piperazin-1-ium iodide(Pzol),comprising a piperazine iodide and a terminated hydroxyl group,which was applied for post-treating the perovskite *** findings reveal that Pzol reacts with and consumes residual PbX_(2.(X:I or Br)to form a 2. perovskite component,thereby eliminating Pb^(0)defects,while the terminated hydroxyl group in PZOI can also passivate uncoordinated Pb^(2.).Consequently,the shallow/deep-level defect densities of the 2./3D perovskite film were significantly reduced,leading to an enhanced PCE of single-junction 2./3D wide-bandgap perovskite solar cells to 18.18% with a reduced energy loss of 40 ***,the corresponding perovskite/organic TSCs achieved a remarkable PCE of 2..05% with enhanced operational stability(T_(90)~500h).

关键词： Perovskite/organic tandem solar cell Defect states Piperazine ion salt Energy conversion Low-dimensional materials Perovskitesolar cells 2./3D perovskites Efficiency

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Investigation of the differences between the “COLD” and “HOT” nature of Coptis chinensis Franch and its processed materials based on animal’s temperature tropism

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Science china(Life sciences) 2009年第11期52卷 1073-1080页

作者： zhou CanPing1,2. wang JiaBo1, {2. XueRu1,2. ZHAO YanLing1, xia XinHua2. ZHAO HaiPing1, REN YongShen1 & xiaO xiaoHe1 1 china Military institute of chinese Meteria Medica, 302.Military Hospital of china, beijing 100039, china 2.College of Pharmaceutical {12., Hunan University of chinese Medicine, Changsha 4102.8, china china Military institute of {2. Meteria Medica 302 Military Hospital of China Beijing 100039 China College of Pharmaceutical sciences Hunan University of Chinese Medicine Changsha 410208 China

The description and differentiation of the so-called "Cold" and "Hot" natures, the primary "Drug Naure" of chinese medicine, is the focus of theoretical research. In this study, the divergency between the "Cold" and the "Hot" natures was investigated through examining the temperature tropism of mice affected by Coptis chinensis Franch and its processed materials by using a cold/hot plate differentiating technology. After exposure to C. chinensis Franch, the macroscopic behavioral index of the remaining rate (RR) on a warm pad (40℃ ) significantly increased (Pchinese medicine may possibly reflect in an ethological way for the changes of animal’s temperature tropism which might be internally regulated by the body’s energy metabolism.

关键词： "Cold" and "Hot" natures of chinese medicine evaluation temperature tropism ethology drug process Coptis chinensis Franch

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Impact of frailty on short-term postoperative outcomes in patients undergoing colorectal cancer surgery:A systematic review and meta-analysis

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World Journal of Gastrointestinal Surgery 2024年第3期16卷 893-906页

作者： Yao zhou xiao-Lei {2. Hong-xia Ni Tian-Jing Shao Ping wang Department of Operating Room Affiliated Tumor Hospital of Nantong University&Nantong Tumor HospitalNantong 226361Jiangsu ProvinceChina Department of Gastrointestinal Surgery The Affiliated Tumor Hospital of Nantong University&Nantong Tumor HospitalNantong 226361Jiangsu ProvinceChina

BACKGROUND Colorectal cancer is a major global health challenge that predominantly affects older *** management,despite advancements,requires careful consideration of preoperative patient status for optimal *** To summarize existing evidence on the association of frailty with short-term postoperative outcomes in patients undergoing colorectal cancer *** A literature search was conducted using PubMed,EMBASE and Scopus databases for observational studies in adult patients aged≥18 years undergoing planned or elective colorectal surgery for primary carcinoma and/or secondary *** studies that conducted frailty assessment using recognized frailty assess-ment tools and had a comparator group,comprising nonfrail patients,were *** effect sizes were reported as weighted mean difference or relative risk(RR)with 95%confidence intervals(CIs).RESULTS A total of 2. studies were *** with nonfrail patients,frailty was associated with an increased risk of mortality at 30 d(RR:1.99,95%CI:1.47-2.69),at 90 d(RR:4.76,95%CI:1.56-14.6)and at 1 year(RR:5.73,95%CI:2.74-12.0)of follow *** patients had an increased risk of any complications(RR:1.81,95%CI:1.57-2.10)as well as major complications(Clavien-Dindo classification grade≥III)(RR:2.87,95%CI:1.65-4.99)compared with the control *** risk of reoperation(RR:1.18,95%CI:1.07-1.31),readmission(RR:1.70,95%CI:1.36-2.12.,need for blood transfusion(RR:1.67,95%CI:1.52.1.85),wound complications(RR:1.49,95%CI:1.11-1.99),delirium(RR:4.60,95%CI:2.31-9.16),risk of prolonged hospitalization(RR:2.09,95%CI:1.2.-3.60)and discharge to a skilled nursing facility or rehabilitation center(RR:3.19,95%CI:2.0-5.08)was all higher in frail *** Frailty in colorectal cancer surgery patients was associated with more complications,longer hospital stays,higher reoperation risk,and increased *** frailty assessment appears crucial for tailored surgical managem

关键词： Frailty Frail adults Colorectal surgery Colorectal cancer Complications Mortality Survival Slinical outcomes Meta-analysis

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Selective sintering of magnesia-calcia materials by utilizing hot spots during induction sintering process

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Journal of Iron and Steel Research International 2024年第8期31卷 1914-1922页

作者： Yun-jie Dong zhou-fu wang Hao Liu Yan Ma Xi-tang wang Nian Yi Zhong-feng xia Yu-xuan Zhu Cheng-ji Deng Ling zhang The state key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan 430081HubeiChina School of Materials and Metallurgy University of Science and Technology LiaoningAnshan 110325LiaoningChina

Magnesia-calcia refractories are widely used in the production process of clean steel due to their excellent high-tem-perature stability,slag resistance and ability to purify molten ***,there are still problems such as difficult sintering and easy ***-calcia materials with various calcium oxide contents were prepared by using induction sintering,and the sintering property combined with the hydration resistance of the materials was *** experimental results showed that the magnesia-calcia materials prepared under induction field had higher density,microhardness and hydration *** particular,the relative density of induction sintered magnesia-calcia materials with 50 mo1%CaO was greater than 98%,and the average grain size of CaO was 4.56μm,which was much larger than that of traditional sintered *** order to clarify the densification and microstructure evolution mechanism of the magnesia-calcia materials,the changes in temperature and magnetic field throughout the sintering process were analyzed by using finite element *** results showed that the larger heating rate and higher sintering temperature under the induction sintering mode were beneficial to the rapid *** addition,the hot spots generated within the material due to the difference in high-temperature electric conductivity between MgO and CaO were the critical factor to realize selective sintering in MgO-CaO system,which provides a novel pathway to solve the problem of difficult sintering and control the microstructure of high-temperature composite material used in the field of high-purity steel smelting.

关键词： Magnesia-calcia material Induction sintering Hot spot Selective sintering Density Microstructure control Hydration resistance

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Heat exposure and hospitalizations for chronic kidney disease in china: a nationwide time series study in 2.1 major chinese cities

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Military Medical Research 2024年第4期11卷 469-478页

作者： Fu-Lin wang Wan-zhou wang Fei-Fei zhang Su-Yuan Peng Huai-Yu wang Rui Chen Jin-Wei wang Peng-Fei Li Yang wang Ming-Hui Zhao Chao Yang Lu-xia zhang institute of Medical Technology Peking University Health Science CenterBeijing 100191China National institute of Health Data Science at Peking University Beijing 100191China Department of Occupational and Environmental Health sciences School of Public HealthPeking UniversityBeijing 100191China Renal Division Department of MedicinePeking University First HospitalPeking University Institute of NephrologyBeijing 100034China Research Units of Diagnosis and Treatment of Immune-Mediated Kidney Diseases Chinese Academy of Medical SciencesBeijing 100034China Advanced institute of Information Technology Peking UniversityHangzhou 311215China National Climate Center China Meteorological AdministrationBeijing 100081China Peking-Tsinghua Center for Life sciences Beijing 100034China

Background:Climate change profoundly shapes the population health at the global ***,there was still insufficient and inconsistent evidence for the association between heat exposure and chronic kidney disease(CKD).Methods:In the present study,we studied the association of heat exposure with hospitalizations for cause-specific CKD using a national inpatient database in china during the study period of hot season from 2.15 to *** time-series regression models and random-effects Meta-analysis were developed to estimate the city-specific and national averaged associations at a 7 lag-day span,***:A total of 768,12. hospitalizations for CKD was recorded during the study *** results showed that higher temperature was associated with elevated risk of hospitalizations for CKD,especially in sub-tropical *** a 1℃ increase in daily mean temperature,the cumulative relative risks(RR)over lag 0-7 d were 1.008[95% confidence interval(CI)1.003-1.012.for *** attributable fraction of CKD hospitalizations due to high temperatures was 5.50%.Stronger associations were observed among younger patients and those with obstructive *** study also found that exposure to heatwaves was associated with added risk of hospitalizations for CKD compared to non-heatwave days(RR=1.116,95%CI 1.069-1.166)above the effect of daily mean ***:Short-term heat exposure may increase the risk of hospitalization for *** findings provide insights into the health effects of climate change and suggest the necessity of guided protection strategies against the adverse effects of high temperatures.

关键词： Chronic kidney disease Hospitalization Climate change Temperature Time-series study

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Mannanase improves the growth performance of broilers by alleviating inflammation of the intestinal epithelium and improving intestinal microbiota

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动物营养学报 2024年第1期16卷 376-394页

作者： xiaodan zhang Huiping Xu Lu Gong Jiao {2. Jianyang Fu Zengpeng Lv Liangjuan zhou Xuejun Li Qiong Liu Pingyu xia Yuming Guo state key Laboratory of Animal Nutrition and Feeding College of Animal Science and Technology China Agricultural University Beijing 100193 China beijing Strowin Biotechnology Co. Ltd. Beijing 100094 China

This experiment aimed to discuss and reveal the effect and mechanism of mannanase on intestinal inflammation in broilers triggered by a soybean meal diet. In this experiment, 384 Arbor Acres broilers at 1 d old were randomly divided into 3 treatment groups. The broilers were fed a corn-soybean meal basal diet, a low-energy diet (metabolizable energy reduced by 50 kcal/kg), and a low-energy diet supplemented with 100 mg/kg mannanase for 42.d. The low-energy diet increased feed conversion ratio from 0 to 42.d, reduced ileal villus height and villus height-to-crypt depth ratio and upregulated the expression of nuclear factor kappa B ( NF-κB ) in the ileum ( P P P CLDN1 ) and zonula occludens-1 ( ZO-1 ) in the jejunum at 2. d, downregulated the expression of ilealNF-κB , and increased the content of isobutyric acid in the cecum of broilers ( P Lactobacillus reuteri in the ileum of broilers. The addition of mannanase increased the relative abundance ofLactobacillus-KC45b andLactobacillus johnsonii in broilers. Furthermore, a low-energy diet reduced the relative abundance ofButyricicoccus in the intestine of broilers and inhibited oxidative phosphorylation and phosphoinositol metabolism. Mannanase increased the relative abundance ofOdoribacter , promoted energy metabolism and N-glycan biosynthesis, and increased the activities of GH3 and GH18. It is concluded that mannanase could improve the growth performance of broilers by reducing the expression ofNF-κB in the ileum, increasing the production of SCFA in the cecum, suppressing intestinal inflammation, balancing the intestinal microbiota, reducing damage to the intestinal barrier, and improving the efficiency of nutrient utilization to alleviate the adverse effects caused by the decrease in dietary energy *** The PDF

关键词： Broiler Low-energy diet Mannanase Intestinal inflammatory response Intestinal microbiota Metagenome

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