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Microclimatic parameters affect Cladosporium rot development and berry quality in table grapes

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Horticultural Plant Journal 2022年第2期8卷 171-183页

作者： Dhanasekaran Solairaj Ngolong Ngea Guillaume Legrand Qiya Yang Jizhan Liu hongyin zhang School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina Key Laboratory of Modern Agricultural Equipment and Technology Ministry of EducationJiangsu UniversityZhenjiang 212013JiangsuChina

Cladosporium cladosporioides is an emerging pathogenic fungus that causes Cladosporium rot in postharvest table grapes(Vitis vinifera). However,studies investigating the infection process of C. cladosporioides are lacking. Therefore, this study aimed to elucidate the infection process by investigating the influence of microclimatic parameters(temperature, wetness and fungal age) in C. cladosporioides pathogenesis, activities of grape defense-related enzymes and grape quality during the infection. C. cladosporioides effectively infects grapes by developing distinct colonies on the artificial wounds and berry surfaces, completing its life cycle within 48 h. The C. cladosporioides disease incidence optimally occurred at 20 ℃ and 25 ℃. Wetness played an influential role in the infectivity of C. cladosporioides and 7-day-old C. cladosporioides resulted in the most serious disease incidence of table grapes. As a result of infection, the quality of grapes was affected, including berry weight, p H, titratable acidity(TA), total soluble solids(TSS), and ascorbic acid level. This infection also induced defense-related enzymes, including polyphenoloxidase(PPO), peroxidase(POD), phenylalanine ammonialyase(PAL), and catalase(CAT), at certain times. The findings of this study demonstrated that Cladosporium rot development depended on the microclimatic parameters of grapes, significantly affected the grape quality and induced grape's defense-related enzymes.

关键词： Cladosporium cladosporioides Table grape Pathogenesis Infection

A prospective study (SCOPE) comparing the cardiometabolic and respiratory effects of air pollution exposure on healthy and pre-diabetic individuals

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Science China(Life Sciences) 2018年第10期61卷 46-56页

作者： Yanwen Wang Yiqun Han Tong Zhu Weiju Li hongyin zhang BIC-ESAT and SKL-ESPC College of Environmental Sciences and Engineering Peking University Peking University Hospital Peking University

Air pollution is known to be a major risk factor for cardiopulmonary disease, but this is unclear for cardiometabolic disease (***). This is of considerable public health importance, given the nationwide epidemic of diabetes, accompanied by severe air pollution, in China. The evidence so far remained inadequate to answer questions of whether individuals with cardiometabolic dysfunctions are susceptible to air pollution and whether air pollution exacerbates diabetes development via certain biological pathways. In this manuscript, we summarize the results and limitations of studies exploring these two topics and elaborate our design of a prospective panel study (SCOPE) as a solution. We assessed and compared the health effect of air pollution among pre-diabetic individuals and matched healthy controls through four repeated clinical visits over 1 year. Comprehensive evaluation was made to both health endpoints and exposure. The primary biomarkers were assessed to reveal the impact on multiple biological pathways, including glycolipid metabolism and insulin resistance, endothelial function, and inflammation. Detailed chemical and size fractional components of particulate matter were measured in this study, along with the application of personal *** work should increase our understanding of how air pollution affects individuals with cardiometabolic dysfunction and the underlying mechanisms.

关键词： panel study air pollution diabetes susceptibility cardiometabolic effect

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南瓜豆奶饮料的研制

食品工业科技 2000年第1期21卷 44-46页

作者：张红印郑州牧业工程高等专科学校食品工程系郑州450008

以南瓜汁、豆奶为主要原料，配以甜味剂、稳定剂、豆奶香精等，经调配、均质、杀菌等处理，制成一种色泽橙黄、气味芳香、酸甜适口、营养丰富的南瓜豆奶复合饮料。采用“正交试验”分析法，以感官评分为标准，确定了产品的配方；以稳定... 详细信息

以南瓜汁、豆奶为主要原料，配以甜味剂、稳定剂、豆奶香精等，经调配、均质、杀菌等处理，制成一种色泽橙黄、气味芳香、酸甜适口、营养丰富的南瓜豆奶复合饮料。采用“正交试验”分析法，以感官评分为标准，确定了产品的配方；以稳定性为标准，确定了乳化剂、稳定剂的配比。

关键词：南瓜汁豆奶配方稳定性研制保健饮料

Transcriptome analysis of postharvest pear（Pyrus pyrifolia Nakai）in response to Penicillium expansum infection

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Transcriptome analysis of postharvest pear（Pyrus pyrifolia ...

中国植物病理学会2021年学术年会

作者： XU Meiqiu zhang Xiaoyun ZHAO Lina YANG Qiya WANG Kaili zhang hongyin School of Food and Biological Engineering Jiangsu University

In pears,blue mold decay caused by Penicillium expansum,is a destructive postharvest disease around the world,which leads to stasis of the pear industry and creates huge economic *** previous research work proved that *** mainly secretes cell wall degrading enzymes（CWDEs） to infect *** further understand the molecular basis of *** infection and disease progression,in the present study we have tried to investigate the transcriptome of pears infected with *** differentially expressed genes（DEGs） identified from the RNA-seq results were clustered into 2 upward trends and 6 downward trends according to their gene expression *** of Gene ontology（GO） terms in downward and upward trend profiles evidenced that the enriched GO terms（included metabolic processes for L-phenylalanine,aromatic amino acid family and ethylene,etc.）,found in upward trend profiles were more relevant to pear ***,the Kyoto Encyclopedia of Genes and Genomes（KEGG） results revealed that pears can produce a complex defense response against *** ***,*** produced CWDEs to establish infection,which triggers relevant transduction pathways in *** pathways led to further activation of the secondary regulatory ***,some metabolites were synthesised to defense against *** general,the information obtained in this study will be supportive to develop new strategies to control fungal diseases.

关键词： Pear Penicillium expansum RNA-seq Defense mechanism Transcription

基于临界模态的DCM-LCC谐振变换器的归一化分析与设计

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电工技术学报 2019年第1期34卷 103-115页

作者：张洪寅童朝南王泽庭北京科技大学自动化学院北京100083 北方工业大学电力电子与电气传动北京市工程研究中心北京100144

为了研究电感电流断续模态(DCM)下LCC串并联谐振变换器的增益特性,通过谐振回路增益和串并联谐振电容比两个参数,建立了不同工作模态下LCC谐振回路的归一化模型。通过引入能量守恒原理及临界电流模态(CRM),推导了一种基于临界模态的归... 详细信息

为了研究电感电流断续模态(DCM)下LCC串并联谐振变换器的增益特性,通过谐振回路增益和串并联谐振电容比两个参数,建立了不同工作模态下LCC谐振回路的归一化模型。通过引入能量守恒原理及临界电流模态(CRM),推导了一种基于临界模态的归一化谐振元件参数设计模型。该模型准确简单,简化了谐振参数的设计。根据推导结果得到不同工作模态下的LCC谐振回路的频率-增益特性曲线,便于对谐振回路的输出特性进行预判。最后以一台额定输出6 kW/6 kV的高压电源样机为例,详细给出了参数设计过程并进行实验验证,实验结果验证了所建立的谐振元件的归一化设计模型的准确性。谐振回路的增益特性曲线的计算、仿真及实验结果对应良好,证明了所述的谐振回路的频率-增益特性曲线的有效性。

关键词： LCC串并联谐振临界模态电流断续模态软开关归一化分析

Postharvest biological control of Fusarium rot and the mechanisms involved in induced disease resistance of asparagus by Yarrowia lipolytica

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Postharvest biological control of Fusarium rot and the mecha...

中国植物病理学会2021年学术年会

作者： Esa Abiso Godana zhang Xiaoyun HU Wanying ZHAO Lina GU Xiangyu zhang hongyin School of Food and Biological Engineering Jiangsu University School of Grain Science and Technology Jiangsu University of Science and Technology

The biocontrol efficacy of Yarrowia lipolytica against natural decay caused by Fusarium rot of asparagus,and the mechanism of *** inducing resistance of asparagus were studied by the activities of defense-related enzymes and transcriptome *** indicated that *** had significant biocontrol efficacy against Fusarium rot of asparagus by elevating activities of defense-related enzymes including polyphenol oxidase(PPO),peroxidase(POD),phenylalanine ammonia lyase(PAL),catalase(CAT),glutathione reductase(GR) and superoxide dismutase(SOD).Meanwhile,the transcriptome analysis revealed that signal transduction pathways of jasmonate(JA) and salicylic acid(SA) were triggered in asparagus by *** signaling was activated(CML19),and reactive oxygen burst(RBOHE、RBOHF) was *** changes further mediated the expression of downstream defense-related genes including PR1 genes,PGIP2,PLP2 and ***,GST and genes related to antioxidation(PER genes,PNC1,Sb03 g046810) and secondary metabolites(CCR,CAD,DIR21,PAL,PER genes,CYP75 B2,CYP73 A13,UGT92 A1,UGT73 C6,F3 H-1,CCoAMT5,ALDH2 C4 and BGLU12) were induced,leading to the accumulation of flavonoids and ***,genes involved in EMP-TCA pathway(FRK2,PFK3 and MSTRG.32630) and related to cell wall and membrane(SBHl,SBH2,LRX and PERK) were *** conclusion,the present study contributes to the understanding of mechanisms behind the induced resistance of asparagus by antagonistic yeast,and would help to develop new disease control strategiesfor post-harvest fruits.

关键词： asparagus Fusarium rot disease resistance

热水处理对草莓采后病害的抑制作用及对贮藏品质的影响

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农业工程学报 2007年第8期23卷 270-273页

作者：张红印王雷姜松董英张华江苏大学食品与生物工程学院镇江212013 鲁东大学应用技术学院烟台264025

研究热水处理对草莓采后病害的抑制作用及对贮藏品质的影响。在预试验的基础上,分别采用38℃、15min,44℃、10min,48℃、5min对新鲜草莓进行热处理,处理后的草莓置于常温下((20±1)℃),每隔12h观察草莓的腐烂情况,并对草莓品质指标进行... 详细信息

研究热水处理对草莓采后病害的抑制作用及对贮藏品质的影响。在预试验的基础上,分别采用38℃、15min,44℃、10min,48℃、5min对新鲜草莓进行热处理,处理后的草莓置于常温下((20±1)℃),每隔12h观察草莓的腐烂情况,并对草莓品质指标进行检测。结果表明:热水处理能够有效地起到抑制草莓采后病害的作用,热水处理对草莓的失重率、硬度、可滴定酸度等水果品质没有显著的不利影响,热水处理后草莓的细胞膜透性与对照相比没有升高。

关键词：热水处理草莓采后病害抑制品质细胞膜透性

Effect of Aureobasidium pullulans S-2 on the postharvest microbiome of tomato during storage

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Effect of Aureobasidium pullulans S-2 on the postharvest mic...

中国植物病理学会2021年学术年会

作者： SHI Yu YANG Qiya ZHAO Qianhua zhang Xiaoyun WANG Kaili ZHAO Lina zhang hongyin School of Food and Biological Engineering Jiangsu University

Biological control of fruit postharvest diseases by antagonistic microorganisms has been considered an effective alternative to chemical *** influence of microbial antagonists on fruitassociated microbiome will provide a new perspective for in-depth study of the antagonistic *** this study,the biocontrol efficacy of *** S-2 against postharvest diseases of tomatoes was ***,the fungal and bacterial microbiota on tomato surfaces were examined by highthroughput *** S-2 can significantly inhibit the decay rate,maintain fruit firmness and reduce weight loss of *** addition,the treatment group can maintain higher titratable acid,ascorbic acid and lycopene than the control *** using *** S-2,more dramatic changes were observed in fungal diversity than bacterial in the *** was significantly enriched in the treatment group,while Cladosporium,Mycosphaerella,Alternaria and Penicillium were deficient compared with the control ***,Brevibacterium,Brachybacterium,Serratia,Glutamicibacter and Pseudomonas also had significant differences between the two *** study demonstrated that the application of *** S-2 resulted in alterations in the bacterial and fungal community and that could inhibit pathogens and decrease fruit disease *** provides new insights into the dynamics of the tomato’s surface microbiome after microbial antagonist treatment.

关键词： Aureobasidium pullulans Tomatoes Biocontrol Fungal community Bacterial community

The Glycoside Hydrolase(eglB) Gene Contributes to Penicillium expansum Pathogenicity During Postharvest Pear Infection

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The Glycoside Hydrolase(eglB) Gene Contributes to Penicilliu...

中国植物病理学会2023年学术年会

作者： XU Meiqiu YA Huiyuan LI Jingyu zhang Xiaoyun ZHAO Lina WANG Duoduo zhang hongyin College of Food and Drug Luoyang Normal University School of Food and Biological Engineering Jiangsu University

The blue mold caused by Penicillium expansum is the main cause of postharvest loss of *** for disease control greatly depend on understanding the mechanisms of pathogen-fruit *** previous research found that the glyc;oside hydrolase family genes in pear showed a significant up-regulated expression trend with prolongation of pathogen infection,A member from glycoside hydrolase gene family Beta-glucosidase lb(eglB) had the highest up-regulated fold *** hydrolases are enzymes with roles including the degradation of plant cell wall polymers including cellulose and *** gene eglB shows high homology with glycoside hydrolase superfamily in *** analysis of eglB was performed by gene knockout and *** showed that deletion of eglB had no effect on colony morphology and microscopic morphology of ***,loss of function of eglB resulted in a reduction in the production of fungal hyphae,thus inhibiting sporulation of ***,the deletion of eglB(AeglB) reduced the pathogenicity of *** in ***(complementation of eglB(ΔeglB-C) restored the growth,conidia production,and virulence of ΔeglB mutant strain to the level of wild-type *** findings indicate that eglB contributes to growth and pathogenicity in ***.

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