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Hsa-miR-1246, hsa-miR-320a and hsa-miR-196b-5p inhibitors can reduce the cytotoxicity of Ebola virus glycoprotein in vitro

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Science China(Life Sciences) 2014年第10期57卷 959-972页

作者： SHENG MiaoMiao ZHONG Ying CHEN Yang DU JianChao JU XiangWu ZHAO Chen zhang guigen zhang LiFang LIU KangTai YANG Ning XIE Peng LI DangSheng zhang Michael Q. JIANG ChengYu State Key Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Department of Biochemistry and Molecular Biology Peking Union Medical College Tsinghua University MOE Key Laboratory of Bioinformatics and Bioinformatics Division Center for Synthetic and System Biology TNLIST/Department of Automation Tsinghua University Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Department of Molecular and Cell Biology Center for Systems Biology The University of Texas State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy West China Hospital Sichuan University

Ebola virus （EBOV） causes a highly lethal hemorrhagic fever syndrome in humans and has been associated with mortality rates of up to 91% in Zaire, the most lethal strain. Though the viral envelope glycoprotein （GP） mediates widespread inflammation and cellular damage, these changes have mainly focused on alterations at the protein level, the role of microRNAs （miRNAs） in the molecular pathogenesis underlying this lethal disease is not fully understood. Here, we report that the miRNAs hsa-miR-1246, hsa-miR-320a and hsa-miR-196b-5p were induced in human umbilical vein endothelial cells （HUVECs） following expression of EBOV GP. Among the proteins encoded by predicted targets of these miRNAs, the adhesion-related molecules tissue factor pathway inhibitor （TFPI）, dystroglycan! （DAG1） and the caspase 8 and FADD-like apoptosis regulator （CFLAR） were significantly downregulated in EBOV GP-expressing HUVECs. Moreover, inhibition of hsa-miR-1246, hsa-miR-320a and hsa-miR-196b-5p, or overexpression of TFPI, DAG1 and CFLAR rescued the cell viability that was induced by EBOV GP. Our results provide a novel molecular basis for EBOV pathogenesis and may contribute to the development of strategies to protect against future EBOV pandemics.

关键词： Ebola virus glycoprotein microRNAs cytotoxicity

Stereospecific Assembly of Trisubstituted Alkenes via Photoinduced Nitrogen-Centered Radical-Triggered C—C Bond Cleavage/Functionalization of Oxime Esters

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Chinese Journal of Chemistry 2024年第12期42卷 1399-1406页

作者： Yu Bao Zhi-Jie Song Jin-Long Dai Shenghu Yan Yue zhang Jia-Yin Wang guigen Li School of Pharmacy Changzhou UniversityChangzhouJiangsu 213164China Department of Chemistry and Biochemistry Texas Tech University79409USA

Comprehensive Summary A general and convenient photoredox-catalyzed acylation and alkylcyanation of MBH acetates has been established,enabling the assembly of the C(sp2)–C(sp3)bond by a nitrogen-centered radical strategy for the synthesis of trisubstituted alkenes in moderate to excellent chemical yields(48 examples in total).The reaction of MBH acetates with acyl(indanone)oxime esters afforded trisubstituted alkenes containing 1,4-dicarbonyl ***,the use of Eosin Y as a photocatalyst in the catalytic system resulted in the formation of distal cyano group-anchored trisubstituted alkenes via deconstructive functionalization of cycloketone oxime ***,these resulting 1,4-dicarbonyl compounds could be applied to late-stage transformations,providing important methods for the synthesis of dihydropyridazin-3(2H)-one.

关键词： Photocatalysis Radical reactions MBH acetates Oxime esters Stereoselectivity Acylation Alkylation Green chemistry

Aggregation-Induced Catalysis: Asymmetric Catalysis with Chiral Aggregates

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Research 2024年第1期2023卷 525-532页

作者： Yao Tang Yu Wang Qingkai Yuan Sai zhang Jia-Yin Wang Shengzhou Jin Ting Xu Junyi Pan Kazimierz Surowiec guigen Li Department of Chemistry and Biochemistry Texas Tech UniversityLubbockTX 79409-1061USA School of Chemistry and Chemical Engineering Nanjing UniversityNanjing 210093China School of Pharmacy Continuous Flow Engineering Laboratory of National Petroleum and Chemical IndustryChangzhou UniversityChangzhouJiangsu 213164China

So far,there have been 4 methods to control chirality including the use of chiral auxiliaries,reagents,solvents,and catalysts documented in literature and *** them,asymmetric catalysts are normally divided into homogeneous and heterogeneous *** this report,we present a new type of asymmetric control—asymmetric catalysis via chiral aggregates that would not belong to the above *** new strategy is represented by catalytic asymmetric dihydroxylation reaction of olefins in which chiral ligands are aggregated by taking advantage of typical aggregation-induced emission systems containing tetrahydrofuran and H_(2)O *** was proven that the chiral induction can be enhanced from er of 78:22 to 97:3 simply by changing the ratios of these 2 cosolvents.

关键词： chiral asymmetric Chiral

Controlled generation of cell–laden hydrogel microspheres with core–shell scaffold mimicking microenvironment of tumor

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Chinese Physics B 2018年第12期27卷 572-579页

作者： Yuenan Li Miaomiao Hai Yu Zhao Yalei Lv Yi He Guo Chen Liyu Liu Ruchuan Liu Department of Physics Chongqing UniversityChongqing 401331China Department of Biomedical Engineering University of KentuckyLexingtonKY40506USA

Development of an in vitro three-dimensional(3D) model that closely mimics actual environment of tissue has become extraordinarily important for anti-cancer study. In recent years, various 3D cell culture systems have been developed,with multicellular tumor spheroids being the most popular and effective model. In this work, we present a microfluidic device used as a robust platform for generating core–shell hydrogel microspheres with precisely controlled sizes and varied components of hydrogel matrix. To gain a better understanding of the governing mechanism of microsphere formation,computational models based on multiphase flow were developed to numerically model the droplet generation and velocity field evolution process with COMSOL Multiphysics software. Our modeling results show good agreement with experiments in size dependence on flow rate as well as effect of vortex flow on microsphere formation. With real-time tuning of the flow rates of aqueous phase and oil phase, tumor cells were encapsulated into the microspheres with controllable core–shell structure and different volume ratios of core(comprised of alginate, Matrigel, and/or Collagen) and shell(comprised of alginate). Viability of cells in four different hydrogel matrices were evaluated by standard acridine orange(AO) and propidium iodide(PI) staining. The proposed microfluidic system can play an important role in engineering the in vitro micro-environment of tumor spheroids to better mimic the actual in vivo 3D spatial structure of a tumor and perfect the 3D tumor models for more effective clinical therapies.

关键词： microfluidics core–shell scaffold phase field method tumor spheroids

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基于PC平台的数字视频制作与应用

核电子学与探测技术 1999年第4期19卷 302-305页

作者：张桂根周华范颖中国科学院上海原子核研究所上海市嘉定教师进修学院

简述了数字视频的发展状况，并详细介绍了一种基于ＰＣ平台的小型广播级电脑视频制作系统的硬件配置、技术规格以及应用软件。

关键词：数字视频多媒体微机非线性编辑制作

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北斗/GNSS多频率码偏差估计与分析

大地测量与地球动力学 2024年第8期44卷 797-803页

作者：李文溢张启元聂桂根武汉大学卫星导航定位技术研究中心武汉市430079

介绍多系统多频码偏差估计的方法和步骤,并验证基于多系统组合电离层建模估计频间码偏差的效果。利用2019-01~2023-07的MGEX网观测数据逐日解算各类码偏差,并统计和分析各类码偏差精度。结果表明,解算的多系统多频OSB平均周稳定性可达0.... 详细信息

介绍多系统多频码偏差估计的方法和步骤,并验证基于多系统组合电离层建模估计频间码偏差的效果。利用2019-01~2023-07的MGEX网观测数据逐日解算各类码偏差,并统计和分析各类码偏差精度。结果表明,解算的多系统多频OSB平均周稳定性可达0.07~0.26 ns,其中BDS-2和BDS-3的多频OSB平均周稳定性分别为0.17~0.26 ns和0.13~0.21 ns,各类型DCB与DLR、CAS产品的一致性在0.3 ns以内。本文方法不依赖于外部事后产品,能以较快的时效性和较高的精度解算码偏差。

关键词：全球电离层建模码偏差 MGEX GNSS

阑尾口息肉的临床特征及内镜下超级微创治疗效果

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中华医学杂志 2024年第35期104卷 3342-3346页

作者：贺胜铎张尚卿葛超毅张璐陈倩倩滕贵根北京大学第一医院消化内科北京100034 解放军总医院第一医学中心消化学部北京100853

分析阑尾口息肉的临床特征及内镜下超级微创治疗效果。回顾性分析2022年1月1日至2023年12月31日期间在北京大学第一医院以及解放军总医院第一医学中心消化内科切除阑尾口息肉患者临床资料。分析患者的年龄、性别等一般情况,阑尾口息肉分... 详细信息

分析阑尾口息肉的临床特征及内镜下超级微创治疗效果。回顾性分析2022年1月1日至2023年12月31日期间在北京大学第一医院以及解放军总医院第一医学中心消化内科切除阑尾口息肉患者临床资料。分析患者的年龄、性别等一般情况,阑尾口息肉分型,内镜下治疗方式,术后病理以及术后并发症情况。共纳入47例患者,男28例,女19例,年龄35~86(61±12)岁。阑尾口息肉分为4型,0、1、2、3型分别为14、15、12和6例。内镜下形态为颗粒型侧向发育型肿瘤22例。治疗方案中行内镜下黏膜剥离术37例。术后放置阑尾支架1例。息肉病理类型:腺瘤15例,高级别上皮内瘤变10例,黏膜内癌4例,黏膜下癌5例,炎性息肉1例,无蒂锯齿状病变12例。治疗结局中治愈性切除44例。术后无出血、穿孔、急性阑尾炎等并发症。阑尾口息肉病理多为癌前病变,内镜下超级微创切除的方案是安全、有效的。

关键词：息肉阑尾临床特征超级微创

GNSS/INS组合导航中IMU参数设定与优化

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导航定位学报 2024年第3期12卷 154-164页

作者：姚东雨聂桂根樊静张全牛小骥武汉大学卫星导航定位技术研究中心武汉430079

针对惯性测量单元(IMU)参数设置不合理导致全球卫星导航系统(GNSS)/惯性导航系统(INS)组合导航无法充分发挥IMU性能的问题,提出了一套IMU参数设定与优化方案,建立了以GNSS短期中断期间导航漂移误差统计值、理论估计精度一致性以及IMU误... 详细信息

针对惯性测量单元(IMU)参数设置不合理导致全球卫星导航系统(GNSS)/惯性导航系统(INS)组合导航无法充分发挥IMU性能的问题,提出了一套IMU参数设定与优化方案,建立了以GNSS短期中断期间导航漂移误差统计值、理论估计精度一致性以及IMU误差参数估计稳定性为评估对象的考核准则,使优化后的IMU参数可以确保GNSS/INS组合导航解算中状态预测方差阵的准确性,实现预测信息与观测信息权重合理分配,进而保障GNSS/INS组合导航最优性能。实测验证采用ADIS16465和ICM206022款典型车规级和消费级微机电(MEMS)IMU进行性能测试分析,测试结果表明:初始IMU参数与GNSS/INS组合导航算法适配度较低,无法得到最优组合导航结果;优化后,IMU参数在保障组合导航最优性能基础之上,也可实现理论估计精度与实际精度高度一致。

关键词：组合导航惯性测量单元(IMU) 参数优化权重分配全球卫星导航系统(GNSS) 惯性导航系统(INS)

天然丰度氘核磁共振测定一级和二级动力学氘同位素效应(Ⅲ)——对Grignard试剂水解反应的应用

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高等学校化学学报 1989年第6期10卷 664-666页

作者：章本礼李桂根高振衡吴家振南开大学化学系中国科学院生物物理研究所

本文报道了用天然丰度^2HNMR测定一级动力学氘同位素效应的方法。这种方法与现有方法不同，它要求在反应时授氢反应物大大过量于受氢底物，在计算时要考虑由^2HNMR测定的反应物和产物的特定部位同位素比率（D／H）i或化合物的平均同位... 详细信息

本文报道了用天然丰度^2HNMR测定一级动力学氘同位素效应的方法。这种方法与现有方法不同，它要求在反应时授氢反应物大大过量于受氢底物，在计算时要考虑由^2HNMR测定的反应物和产物的特定部位同位素比率（D／H）i或化合物的平均同位素比率（D／H）。我们用这种方法研究了一些Grignard试剂水解的同位素效应。

关键词：同位素效应 Grignard试剂水解反应氘同位素天然丰度氘核磁共振动力学